Abstract:
:Oral squamous cell carcinoma (OSCC) is characterized by high mortality rates. High incidence of local recurrences in the normal-appearing surgical margins of OSCC patients indicates the existence molecular alterations, including DNA methylation, which could not be detectable by histopathologic analysis. The objective of our study was to determine correlation of tumor-related genes hypermethylation detected in histopathologically negative surgical margins with clinical and prognostic parameters. The genes selected for methylation analysis covered a wide range cellular processes including cell cycle control (p16), apoptosis (DAPK and RASSF1A), Wnt signaling (APC, WIF1 and RUNX3), cell-cell adhesion (E-cad), and DNA repair (MGMT and hMLH1). All of 47 patients had histologically confirmed negative surgical margins. For each of patient, samples from primary malignant tissue and the two consecutive surgical margins were taken at the time of surgery. DNA methylation was determined by multiplex nested methylation-specific PCR. Twenty-seven patients were margin-positive for promoter hypermethylation of at least one gene under study. The presence of DAPK promoter hypermethylation detected in surgical margins was associated with the decreased overall survival (p=0.004, log rank test). Multivariate analysis revealed that DAPK promoter hypermethylation in surgical margins is an independent prognostic factor for overall survival, HR=4.105 (1.458-11.555, 95% CI, p=0.007). Hypermethylation of other tumor-related genes under study did not have prognostic significance. These results demonstrate that DNA hypermethylation in histologically negative surgical margins is a frequent event. Promoter hypermethylation of DAPK gene detected in surgical margins may be a useful molecular marker for the poor survival in OSCC patients. Further investigation into the therapeutic potential of these findings in OSCC is warranted.
journal_name
Oral Oncoljournal_title
Oral oncologyauthors
Supic G,Kozomara R,Jovic N,Zeljic K,Magic Zdoi
10.1016/j.oraloncology.2011.05.014subject
Has Abstractpub_date
2011-08-01 00:00:00pages
702-8issue
8eissn
1368-8375issn
1879-0593pii
S1368-8375(11)00191-6journal_volume
47pub_type
杂志文章相关文献
ORAL ONCOLOGY文献大全abstract::Chemotherapeutic regimens incorporating taxanes significantly improve outcomes for patients with squamous cell carcinomas of the head and neck (SCCHN). However, treatment with taxanes is limited by toxicities, including bone marrow suppression and peripheral neuropathies. We proposed that conjugating taxanes to target...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2011.07.029
更新日期:2011-11-01 00:00:00
abstract::The p53 pathway is commonly altered during oral and skin carcinogenesis. The lip is a transition tissue between skin and oral mucosa, which in response to UVB exposure also exhibits alterations in the expression of p53 and p53-related genes that could lead to malignant transformation. To assess if the p53-regulated pr...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2007.11.008
更新日期:2008-09-01 00:00:00
abstract::We evaluate whether p53-reactivating (p53RA) small molecules induce p53-dependent apoptosis in head and neck squamous cell carcinoma (HNSCC), a question that has not been previously addressed in head and neck cancer. PRIMA-1, CP-31398, RITA, and nutlin-3 were tested in four human HNSCC cell lines differing in TP53 sta...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2010.10.011
更新日期:2011-01-01 00:00:00
abstract::Facial Paralysis (FP) profoundly impairs the life of individuals, both functionally and psychosocially. Surgical approaches to treat this condition are myriad, but the ultimate goal is to restore symmetry and movement. Ablative surgery for tumors of the head and neck region are amongst the most common etiologies causi...
journal_title:Oral oncology
pub_type: 杂志文章,评审
doi:10.1016/j.oraloncology.2017.08.013
更新日期:2017-11-01 00:00:00
abstract::Oral cancer models have attempted to demonstrate inhibition of oral carcinogenesis. These models used synthetic carcinogens, lacked a specific mechanism of activity or used non-physiologic doses for carcinogen or inhibitor. To correct these problems the tobacco and environmental carcinogen, dibenzo[a,l]pyrene (DB[a,l]...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2003.12.012
更新日期:2004-07-01 00:00:00
abstract:OBJECTIVES/HYPOTHESIS:We review the use of topical chemoprevention agents in patients with oral potentially malignant disorders (PMD). METHODS:A systematic review of studies on topical chemoprevention agents for oral PMD from 1946 to November 2016 was conducted using the MEDLINE database, Embase, and Cochrane Library....
journal_title:Oral oncology
pub_type: 杂志文章,评审
doi:10.1016/j.oraloncology.2017.02.014
更新日期:2017-04-01 00:00:00
abstract:OBJECTIVES:Diffusion weighted imaging (DWI) is a frequently performed MRI sequence in cancer patients. While previous studies have shown the clinical value of the apparent diffusion coefficient (ADC) for response prediction and response monitoring, less is known about the biological background of ADC. In the tumor micr...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2017.12.001
更新日期:2018-02-01 00:00:00
abstract::In recent years, studies on the malignant potential of oral lichen planus (OLP) provided clinical evidence that patients affected by OLP have an increased risk to develop oral squamous cell carcinoma (OSCC); nevertheless, controversies still exist as to whether OLP has inherent predisposition to become malignant, or n...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/s1368-8375(00)00096-8
更新日期:2001-04-01 00:00:00
abstract:BACKGROUND:The chemokine receptor CCR7 is expressed on lymphocytes and dendritic cells and is responsible for trafficking of these cells in and out of secondary lymphoid organs. It has recently been shown that CCR7 expression is elevated in a number of cancers, including head and neck cancers, and that its expression c...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2018.03.014
更新日期:2018-05-01 00:00:00
abstract::The semaphorins belong to a family of proteins originally shown to be important in axon migration and proper central nervous system development. Recent evidence has demonstrated that semaphorins and their receptors are expressed in tissues outside of the nervous system and represent a widespread signaling system that ...
journal_title:Oral oncology
pub_type: 杂志文章,评审
doi:10.1016/j.oraloncology.2007.10.005
更新日期:2008-06-01 00:00:00
abstract:BACKGROUND:Reconstruction of the tongue and floor of mouth after total/subtotal glossectomy poses a major challenge for reconstructive surgeons. Speech and deglutition after total glossectomy are usually significantly impaired, affecting the quality of life of these patients. Maintaining an adequate volume of the recon...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2020.104660
更新日期:2020-06-01 00:00:00
abstract::The high frequency of second or third primary tumors was first explained by Slaughter et al. with the concept of field cancerisation. Another theory postulates micrometastatic lesions as a reason for this phenomenon. The micronuclei (MN)-assay was evaluated to provide evidence for the concept of field cancerisation an...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/s1368-8375(00)00051-8
更新日期:2000-11-01 00:00:00
abstract:PURPOSE:To assess cause-specific mortality in a large population-based cohort of 14,393 patients treated for squamous cell carcinoma of the oral cavity (OC) or oropharynx (OP) in The Netherlands between 1989 and 2006. PATIENTS AND METHODS:Causes of death were obtained for 94.7% of 9620 patients who had died up to Janu...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2015.10.013
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVES:For over a century, a heated debate existed over the possibility of malignant transformation of oral lichen planus (OLP). We performed this meta-analysis to evaluate the malignant potential of OLP and oral lichenoid lesions (OLL) and investigate the possible risk factors for OLP malignant transformation into...
journal_title:Oral oncology
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.oraloncology.2017.03.012
更新日期:2017-05-01 00:00:00
abstract:BACKGROUND:The increasing occurrence of radiation-induced second primary squamous cell carcinoma of the oral cavity (RISCCO) after radiotherapy for nasopharyngeal carcinoma (NPC) has become a noteworthy complication that can influence long-term survival. This study aimed to analyze the associations of clinicopathologic...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2020.104863
更新日期:2020-06-27 00:00:00
abstract::The recent success of immunotherapies has demonstrated the potency of tumor-specific immune cells in mediating tumor rejection and generating durable tumor immunity. Our understanding of the scientific basis of these responses results from the confluence of a better comprehension of the cancer immunoediting process an...
journal_title:Oral oncology
pub_type: 杂志文章,评审
doi:10.1016/j.oraloncology.2016.09.010
更新日期:2017-08-01 00:00:00
abstract:OBJECTIVE:To identify functional outcome and orbital complication rate of the preserved orbit and to identify predictors of orbital impairment in periorbital free flap reconstruction. MATERIALS AND METHODS:A retrospective review was conducted on patients undergoing orbit-sparing surgery with periorbital free flap reco...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2019.09.023
更新日期:2019-11-01 00:00:00
abstract::The etiology of oral squamous cell carcinoma has been linked to environmental carcinogens, such as activated aromatic heterocyclic radicals and epoxides. Our previous work on implantable and 7,12-dimethylbenz[a]anthracene (DMBA)-induced breast cancer showed that oral glutamine (GLN) inhibited tumor growth possibly thr...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2008.04.008
更新日期:2009-02-01 00:00:00
abstract::Ameloblastoma is a rare human disease of benign neoplasm odontogenic tumor with a lower prevalence but higher recurrence rate. Etiology of ameloblastoma is not fully understood thus lacks implementation of curative treatments. One of the proposed models of evolution of ameloblastoma is related to alteration in DNA dam...
journal_title:Oral oncology
pub_type: 杂志文章,评审
doi:10.1016/j.oraloncology.2020.104804
更新日期:2020-09-01 00:00:00
abstract::Nasopharyngeal carcinoma is well known to be a viral driven malignancy. Despite high loco-regional control rate with modern radiotherapy, distant metastasis remains as a major threat to the patients highlighting the importance of effective systemic therapy. Recent advances in the understanding of tumor biology, host c...
journal_title:Oral oncology
pub_type: 杂志文章,评审
doi:10.1016/j.oraloncology.2013.11.005
更新日期:2014-05-01 00:00:00
abstract:OBJECTIVE:Elective neck dissection (END) for salvage laryngectomy remains controversial due to variability in reported occult nodal metastasis rates and postoperative complications. We performed a meta-analysis to examine the role of END for treatment of the clinically N0 (cN0) neck in the salvage setting. METHODS:A P...
journal_title:Oral oncology
pub_type: 杂志文章,meta分析
doi:10.1016/j.oraloncology.2019.07.008
更新日期:2019-09-01 00:00:00
abstract::An intravenous pseudotumour at a traumatised site of the buccal mucosa of a 68-year-old woman is described. The lesion was composed of reactive spindle cell proliferation, merging of haphazard bizarre cells and inflammatory elements. Cellular morphology and immunohistochemical profile of proliferating cells reflected ...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/s1368-8375(97)00080-8
更新日期:1998-05-01 00:00:00
abstract::Hematogenous metastasis is one of the most important factors determining the outcome of the patients with salivary adenoid cystic carcinoma (SACC). In the present study, we examined expression profile of genes in SACC cell lines to look for molecules responsible for its unique metastatic trait. A transcriptomic microa...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2008.04.009
更新日期:2009-02-01 00:00:00
abstract::Oral lichen planus (OLP) is a chronic inflammatory disease and although classified by WHO as a premalignant condition, the risk for transformation into squamous cell carcinoma of the head and neck (SCCHN) is a matter of great controversy. The p63 gene encodes six different proteins which are required for development o...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2007.08.001
更新日期:2008-07-01 00:00:00
abstract::Tubulopapillary hidradenoma-like tumor of the mandible is extremely rare, with only 3 cases published in the English-language literature. The clinicopathological characteristics and histogenesis of this tumor are unclear. Few pathologists and perhaps fewer clinicians are familiar with this entity, and it is likely und...
journal_title:Oral oncology
pub_type: 信件
doi:10.1016/j.oraloncology.2019.104448
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVES:Head and neck radiotherapy can cause several toxicities, and its management has important treatment implications. Proper information about treatment is crucial to assist patients by preparing them and enhancing their ability to manage their illness. Thus, this study aimed to verify the impact of an education...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2020.104712
更新日期:2020-07-01 00:00:00
abstract::We investigated the knowledge and behaviors of a random sample of 500 primary care physicians (PCPs) regarding oral cancer in Calabria (Italy). 87.6% and 64% indicated tobacco and alcohol use as risk factors, only 31.5% and 2.8% having a prior oral cancer lesion and older age. 60.9% knew that squamous-cell carcinoma i...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2003.10.001
更新日期:2004-05-01 00:00:00
abstract:OBJECTIVES:Human papillomavirus (HPV)-positive tumor status is associated with improved prognosis after disease recurrence in oropharyngeal squamous cell carcinoma (OPSCC). In this study the potential role of survival bias in the relationship between HPV tumor status and the timing of recurrence was evaluated, given co...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2015.10.016
更新日期:2016-01-01 00:00:00
abstract:OBJECTIVES:Induction chemotherapy (IC) followed by chemoradiation (CRT) for locally advanced squamous cell head and neck cancer (SCCHN) remains controversial in the absence of clear evidence to define its role. As part of a prospective, randomised, multicentre study of CRT for stage III/IV laryngeal/hypopharyngeal canc...
journal_title:Oral oncology
pub_type: 杂志文章,多中心研究
doi:10.1016/j.oraloncology.2013.10.012
更新日期:2014-02-01 00:00:00
abstract:OBJECTIVES:Cancer-associated fibroblasts (CAFs) play an important role in tumor progression and are associated with a poor prognosis. Tumor necrosis factor α (TNFα) has been involved in growth and metastasis associated with epithelial-mesenchymal transition (EMT) in many types of cancers. However, the relationship amon...
journal_title:Oral oncology
pub_type: 杂志文章
doi:10.1016/j.oraloncology.2015.08.017
更新日期:2015-12-01 00:00:00