Abstract:
:ZCVI₄-2 is a newly developed furoxan-based nitric oxide-releasing derivative of oleanolic acid. It exhibited strong cytotoxicity against human hepatocellular carcinoma (HCC) in vitro and significantly inhibited the growth of HCC tumors in vivo. However, its low aqueous solubility and toxicity due to the fast release of nitric oxide (NO) in blood challenged its formulation. In the present investigation, the interaction characteristics of ZCVI₄-2 with bovine serum albumin (BSA) were studied by fluorescence spectrometry, synchronous fluorescence spectra and Fourier transform-infrared (FT-IR). It was found that ZCVI₄-2 concentration, temperature and pH had significant effect on the interactions. ZCVI₄-2 was able to bind BSA with high affinity, low temperature and neutral pH favor the binding. The interaction exhibited to be a spontaneous and exothermic process. ZCVI₄-2 was buried in the hydrophobic pocket in subdomain IIB of BSA and the exact binding site was around 3.83 nm in average from Trp²¹². The NO releasing characteristics of nanocomplexes were compared with ZCVI₄-2 solution by Griess Reagent Method. It was found that the release of NO from ZCVI₄-2/BSA nanocomplexes was retarded significantly, thus making ZCVI₄-2 into a BSA-bound nanocomplexes had the great potential to lower the toxicity due to the absence of organic solvents and surfactants and meanwhile the sustained release of NO.
journal_name
Chem Pharm Bull (Tokyo)journal_title
Chemical & pharmaceutical bulletinauthors
Zhang J,Gao Y,Su F,Gong Z,Zhang Ydoi
10.1248/cpb.59.734subject
Has Abstractpub_date
2011-01-01 00:00:00pages
734-41issue
6eissn
0009-2363issn
1347-5223pii
JST.JSTAGE/cpb/59.734journal_volume
59pub_type
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