A bio-telemetric device for measurement of left ventricular pressure-volume loops using the admittance technique in conscious, ambulatory rats.

Abstract:

:This paper presents the design, construction and testing of a device to measure pressure-volume loops in the left ventricle of conscious, ambulatory rats. Pressure is measured with a standard sensor, but volume is derived from data collected from a tetrapolar electrode catheter using a novel admittance technique. There are two main advantages of the admittance technique to measure volume. First, the contribution from the adjacent muscle can be instantaneously removed. Second, the admittance technique incorporates the nonlinear relationship between the electric field generated by the catheter and the blood volume. A low power instrument weighing 27 g was designed, which takes pressure-volume loops every 2 min and runs for 24 h. Pressure-volume data are transmitted wirelessly to a base station. The device was first validated on 13 rats with an acute preparation with 2D echocardiography used to measure true volume. From an accuracy standpoint, the admittance technique is superior to both the conductance technique calibrated with hypertonic saline injections, and calibrated with cuvettes. The device was then tested on six rats with 24 h chronic preparation. Stability of animal preparation and careful calibration are important factors affecting the success of the device.

journal_name

Physiol Meas

authors

Raghavan K,Feldman MD,Porterfield JE,Larson ER,Jenkins JT,Escobedo D,Pearce JA,Valvano JW

doi

10.1088/0967-3334/32/6/007

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

701-15

issue

6

eissn

0967-3334

issn

1361-6579

pii

S0967-3334(11)63598-3

journal_volume

32

pub_type

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