Abstract:
:Insulin secretion was stimulated and cyclic adenosine 3', 5'-monophosphate (cAMP) levels were elevated in isolated rat islets by 27.5 mmol/l glucose. Alloxan caused a dose-dependent decrease in both variables with complete obliteration of insulin release at a concentration of 1.25 mmol/l. D-glucose, in the presence or absence of extracellular calcium, or 3-0-methyl-D-glucose (both at 27.5 mmol/l) protected completely against the effects of alloxan on both glucose-induced insulin release and cAMP Levels. 3-0-Methylglucose did not stimulate insulin secretion or elevate cAMP and did not interfere with glucose-stimulated secretion or elevation of cAMP. When glucose-stimulated insulin release was abolished by alloxan, the metabolism of glucose, determined by the rate of 3H2O formation from [5-3H] glucose, was depressed by 20%. It is concluded that alloxan altered the adenylate cyclase system such that it could no longer be stimulated by glucose. Glucose-stimulated insulin secretion or elevation of cAMP did not appear essential for glucose to protect against alloxan. Protection by 3-0-methylglucose did not appear to be mediated through an alteration of cAMP metabolism. Alloxan did not inhibit glucose-induced insulin secretion by grossly altering glycolysis.
journal_name
Diabetologiajournal_title
Diabetologiaauthors
Zawalich WS,Karl RC,Matschinsky FMdoi
10.1007/BF01225460subject
Has Abstractpub_date
1979-02-01 00:00:00pages
115-20issue
2eissn
0012-186Xissn
1432-0428journal_volume
16pub_type
杂志文章相关文献
DIABETOLOGIA文献大全abstract::The authors have been made aware that the following sentence is incorrect: 'Like IIK7, both ramelteon and tasimelteon have a greater affinity for the MT2 receptor [162].' ...
journal_title:Diabetologia
pub_type: 杂志文章,已发布勘误
doi:10.1007/s00125-018-4577-3
更新日期:2018-05-01 00:00:00
abstract:AIMS/HYPOTHESIS:In the light of a report suggesting that insulin glargine may increase cancer occurrence, the EASD asked us to perform this study. METHODS:We followed 114,841 individuals who had a prescription dispensed for insulin between 1 July and 31 December 2005. From 1 January 2006 to 31 December 2007, we noted ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-009-1444-2
更新日期:2009-09-01 00:00:00
abstract::D600 (2 to 20 muM; alpha-isopropyl-alpha [(N-methyl-N-homoveratril)-gamma-aminopropyl]-3,4,5-trimethoxyphenyl-acetonitril) caused a dose-related, rapid and reversible inhibition of glucose-induced insulin release. It also suppressed the insulinotropic action of a sulphonylurea but failed to affect the enhancing action...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF01221969
更新日期:1976-03-01 00:00:00
abstract::Type 1 diabetes is an immune-mediated disease leading to almost total beta cell destruction and permanent exogenous insulin dependency. The appearance of clinical symptoms is preceded by an asymptomatic preclinical period, the duration of which is highly individual. The emergence of diabetes-associated autoantibodies ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-016-4147-5
更新日期:2017-01-01 00:00:00
abstract::The spontaneous hypertensive rat is an animal model characterized by a syndrome of hypertension, insulin resistance and hyperinsulinaemia. To elucidate whether in analogy to other insulin resistant animal models an inactivity of the insulin receptor kinase or an alteration of the glucose transporter (GLUT 4) level in ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00429089
更新日期:1992-08-01 00:00:00
abstract:AIMS/HYPOTHESIS:Carbohydrate-free diet prevents hyperglycaemia and beta cell destruction in the New Zealand Obese (NZO) mouse model. Here we have used a sequential dietary regimen to dissociate the effects of obesity and hyperglycaemia on beta cell function and integrity, and to study glucose-induced alterations of key...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-010-1973-8
更新日期:2011-03-01 00:00:00
abstract::The eyes, kidneys and pancreas of mice (SJL/J) infected with encephalomyocarditis virus were examined by light and electron microscopy. Diabetic mice with the longest duration (6 months) of diabetes showed marked renal and ocular alterations. Fasting blood glucose levels were 17.8-21.9 mmol/l and glycosuria was presen...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00282717
更新日期:1983-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:We sought to assess the role of coronary artery calcification (CAC) and its progression in predicting incident coronary artery disease (CAD) in individuals with type 1 diabetes using data from the Pittsburgh Epidemiology of Diabetes Complications (EDC) Study. METHODS:The present study examined 292 part...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-018-4764-2
更新日期:2019-02-01 00:00:00
abstract::The effect of interference with diacylglycerol metabolism was investigated in pancreatic mouse islets. In the presence of the diacylglycerol lipase inhibitor RHC 80,267, glucose-induced insulin secretion was reduced 50-60%; whereas carbacholin-induced insulin secretion was unaffected. Addition of the diacylglycerol ki...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00505183
更新日期:1989-02-01 00:00:00
abstract::To test the hypothesis that interaction between genetic, immunological, clinical and metabolic risk factors influences the outcome of Type II (non-insulin-dependent) diabetes mellitus, we examined which of the above factors present at baseline were associated with mortality in 134 Type II diabetic patients followed fo...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051062
更新日期:1998-11-01 00:00:00
abstract:AIMS/HYPOTHESIS:We aimed to describe the prevalence of retinopathy in an aged cohort of Icelanders with and without diabetes mellitus. METHODS:The study population consisted of 4,994 persons aged ≥ 67 years, who participated in the Age, Gene/Environment Susceptibility-Reykjavik Study (AGES-R). Type 2 diabetes mellitus...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-011-2395-y
更新日期:2012-03-01 00:00:00
abstract::Glutathione-insulin transhydrogenase catalyzed the inactivation of insulin by splitting the hormone into A and B chains. We have localized this enzyme immunocytochemically by light microscopy in the pancreas, kidney and liver of both lean and obese (ob/ob) mice and similarly in normal and streptozotocin-diabetic rats....
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00257787
更新日期:1981-11-01 00:00:00
abstract::To determine whether increased oxidative stress in diabetes mellitus is due to an impaired free-radical scavenger function in endothelial cells, GSH-dependent H2O2 degradation in human umbilical vein endothelial cells was studied. The GSH-dependent, NaN3-uninhibitable H2O2-degradation in endothelial cells was reduced ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00398053
更新日期:1994-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:Type 1 diabetes is an autoimmune disorder characterised by loss of insulin-producing beta cells of the pancreas. Progress in understanding the cellular and molecular mechanisms underlying the human disease has been hampered by a dearth of appropriate human experimental models. We previously reported the...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-019-04988-6
更新日期:2019-12-01 00:00:00
abstract:AIMS/HYPOTHESIS:Abnormal rheological properties of erythrocytes, leucocytes and plasma may have a role in the development of diabetic microangiopathy. We hypothesized that changed haemorrheological variables may already be found in children with onset diabetes. METHODS:Erythrocyte deformation (rheoscope), neutrophil d...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051239
更新日期:1999-07-01 00:00:00
abstract:AIMS/HYPOTHESIS:The process of islet isolation can cause chemical and mechanical injury to beta cells. In addition, hyperglycaemia after islet transplantation can compromise beta cell function. The aim of this experiment was to evaluate changes in gene expression in endogenous islets using laser-capture microdissection...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0536-5
更新日期:2007-02-01 00:00:00
abstract::The content of phosphoenolpyruvate (PEP) has been measured in isolated rat islets of Langerhans incubated in vitro. Islet PEP was higher in islets incubated with 16.7 mmol/1 glucose than in islets incubated with zero or 2.8 mmol/1 glucose. Islet PEP content was also increased in islet incubated with 5 mmol/1 D-glycera...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF01234500
更新日期:1977-09-01 00:00:00
abstract:AIMS/HYPOTHESIS:Microvascular complications in the heart and kidney are strongly associated with an overall rise in inflammation. Annexin A1 (ANXA1) is an endogenous anti-inflammatory molecule that limits and resolves inflammation. In this study, we have used a bedside to bench approach to investigate: (1) ANXA1 levels...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-017-4469-y
更新日期:2018-02-01 00:00:00
abstract::Urinary albumin excretion has been assessed in 585 newly-presenting Type 2 (non-insulin-dependent) diabetic patients (aged 53 (8) years, 67% male) at diagnosis with fasting plasma glucose 10.3 (3.2) mmol/l and over 3 years of dietary treatment. Urinary albumin at diagnosis, geometric mean (1 SD interval) corrected for...
journal_title:Diabetologia
pub_type: 杂志文章
doi:
更新日期:1993-10-01 00:00:00
abstract::The antiprotozoal drug pentamidine can be toxic to islet cells in vivo and in vitro. Rat islets were exposed to pentamidine (mesylate and isethionate salts) and six other structurally related diamidines. The beta-cell response to arginine + theophylline was suppressed by pentamidine (10(-2) mmol/l) while the glucagon ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00283144
更新日期:1985-06-01 00:00:00
abstract::Insulin release and 86Rb efflux were studied in perifused rat islets exposed in vitro to alloxan (2 mmol/l) for 5 min. At a low glucose concentration, alloxan transiently increased 86Rb efflux. Alloxan immediately and completely abolished the secretory response to glucose (15 mmol/l) and markedly delayed the reduction...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF01221952
更新日期:1979-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:Non-alcoholic fatty liver disease (NAFLD) has been associated with the metabolic syndrome. However, it is not clear whether insulin resistance is an independent feature of NAFLD, and it remains to be determined which of the in vivo actions of insulin are impaired in this condition. METHODS:We performed...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-005-1682-x
更新日期:2005-04-01 00:00:00
abstract::Non-obese diabetic mice were treated daily with a mixture of gangliosides from day 30 until day 250 of life or until the mice became diabetic. Ganglioside treatment reduced diabetes incidence from 80-90% to 47% and from 20-30% to zero in female and male mice respectively. Gangliosides did not affect the frequency of p...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00277490
更新日期:1988-11-01 00:00:00
abstract:AIMS/HYPOTHESIS:Mitochondrial oxidative phosphorylation (OXPHOS) plays an important role in the pathophysiology of type 2 diabetes. Genes involved in OXPHOS have been reported to be down-regulated in skeletal muscle from patients with type 2 diabetes; however, hepatic regulation is unknown. MATERIALS AND METHODS:We an...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-006-0489-8
更新日期:2007-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:The benefits of moderate alcohol consumption for type 2 diabetes have been postulated to involve a mechanism of improved insulin sensitivity. Fetuin-A, which is known to inhibit insulin signalling, has emerged as a biomarker for diabetes risk. Alcohol consumption may influence circulating fetuin-A conce...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-013-3077-8
更新日期:2014-01-01 00:00:00
abstract:AIMS/HYPOTHESIS:Several publications suggest an association between certain types of insulin and cancer, but with conflicting results. We investigated whether insulin glargine (A21Gly,B31Arg,B32Arg human insulin) is associated with an increased risk of cancer in a large population-based cohort study. METHODS:Data for ...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-011-2312-4
更新日期:2012-01-01 00:00:00
abstract::Clinical and biochemical studies were carried out in 33 patients with diabetes secondary to chronic calcific, non-alcoholic pancreatitis (tropical pancreatic diabetes) and in 35 Type 2 (non-insulin-dependent) diabetic patients and 35 non-diabetic subjects. Despite lower body mass indices, only 25% of patients with tro...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/BF00282238
更新日期:1985-04-01 00:00:00
abstract:AIMS/HYPOTHESIS:We tested the hypothesis that silent coeliac disease is more frequent than expected in both patients with Type I (insulin-dependent) diabetes mellitus and their first-degree relatives. We evaluated how the presence of other autoimmune disorders in diabetic patients and their first-degree relatives is re...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s001250051593
更新日期:2001-02-01 00:00:00
abstract:AIMS/HYPOTHESIS:Glucose-dependent insulinotropic polypeptide (GIP) is a peptide hormone released from gut K cells. While the predominant form is GIP(1-42), a shorter form, GIP(1-30), is produced by pancreatic alpha cells and promotes insulin secretion in a paracrine manner. Here, we elucidated whether GIP(1-30) express...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-015-3842-y
更新日期:2016-03-01 00:00:00
abstract:AIMS/HYPOTHESIS:The aim of this work was to examine the associations of average weight and weight velocity in three growth periods from birth through adolescence with type 2 diabetes incidence. METHODS:Child participants were selected from a 43 year longitudinal study of American Indians to represent three growth peri...
journal_title:Diabetologia
pub_type: 杂志文章
doi:10.1007/s00125-020-05165-w
更新日期:2020-09-01 00:00:00