Research on PEGylation of porcine prothrombin for improving biostability and reducing animal immunogenicity.

Abstract:

:Prothrombin is a vitamin K-dependent serine protease and plays pivotal roles in both procoagulant and anticoagulant pathway of hemostasis. In this study, prothrombin purified from porcine plasma was modified through PEGylation at N-terminal residue using 40 kDa PEG-phenyl-isothiocyanate (PIT-PEG40K). The monoPEGylated prothrombin enhanced biostability and remarkably prolonged circulating half-life in mice as compared with that of the nonmodified prothrombin. Moreover, the immunogenicity of PEGylated prothrombin in mice is significantly decreased compared to nonmodified prothrombin. These studies demonstrated the feasibility of PEGylating prothrombin as a promising way for the development of new prothrombin drugs.

journal_name

Bioorg Med Chem Lett

authors

Zhou JG,Chen YM

doi

10.1016/j.bmcl.2011.04.037

subject

Has Abstract

pub_date

2011-06-01 00:00:00

pages

3268-72

issue

11

eissn

0960-894X

issn

1464-3405

pii

S0960-894X(11)00493-8

journal_volume

21

pub_type

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