Abstract：
BACKGROUND:The complexity of surgical procedure in mouse heterotopic heart transplantation (HHT) has prevented its widespread use. The present study reported a modified technique - splint tubing technique (STT) based on cuff technique (CT). MATERIALS AND METHODS:C57BL/10 and BALB/c mice were performed in syngeneic and allogeneic HHT using STT and CT. The main improvement is that the recipient external jugular vein and common carotid artery were independently opened a mouth and inserted a cannula to avoid the difficult operations of sleeved and everted tube. Graft function was assessed by pulse palpation, echocardiography and histopathologic examination. RESULTS:Ten syngeneic and thirty allogeneic HHT using STT were performed with six graft losses. Ten allogeneic HHT using CT were carried out with two graft losses. Technically successful syngeneic grafts have survived to the pre-specified 30days endpoint with strong contraction. STT significantly shortened operation time compared with CT (32.33±4.21min vs 45.15±4.89min, P<0.05). No significant difference was observed in survival time between two methods. CONCLUSION:STT is easily learned. It reduces the operation difficulty and makes the operation possible for the beginner to master this skill within 1-2weeks. Shorter operation time leads higher operative success rate.
journal_name
Transpl Immunoljournal_title
Transplant immunologyauthors
Li C,Luo L,Lu J,Feng L,Shan J,Long D,Guo Y,Wu W,Li S,Li Ydoi
10.1016/j.trim.2011.03.005subject
Has Abstractpub_date
2011-07-01 00:00:00pages
82-7issue
1eissn
0966-3274issn
1878-5492pii
S0966-3274(11)00027-Xjournal_volume
25pub_type
杂志文章abstract：:TIRC7 delivers essential signals during immune activation as antibodies targeting TIRC7 inhibit lymphocyte proliferation and Th1 cytokine expression in vitro and prolonged kidney and heart allograft survival in vivo. Immunohistochemical analysis of biopsy specimens from human renal allografts undergoing rejection desp...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2006.09.027
更新日期：2006-11-01 00:00:00
abstract：:Induction of donor-specific hyporesponsiveness would minimize the need for intensive immunosuppression in the clinical setting of graft rejection and dendritic cells (DCs) might be useful tools for this purpose. Besides their ability to induce immunogenic T-cell responses, these antigen presenting cells can lead to T-...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2006.03.001
更新日期：2006-06-01 00:00:00
abstract：BACKGROUND:Molecules of the innate immune response are increasingly recognized as important mediators in allograft injury during and after kidney transplantation. We therefore aimed to establish the relationship between the expression of these genes at implantation, during an acute rejection (AR) and on graft outcome. ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2018.02.009
更新日期：2018-06-01 00:00:00
abstract：:Keratinocytes are activated to express MHC class II and ICAM-1 molecules during cutaneous inflammatory reactions. It is controversial how the interaction between these 'nonprofessional' antigen presenting cells (APC) and infiltrating T cells affects the local inflammatory response. To address this issue we analyzed wh...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(93)90059-h
更新日期：1993-01-01 00:00:00
abstract：:In this review, we summarize the work published over the last few years relative to cellular immunological hurdles encountered specifically in pig-to-primate xenotransplantation models. The works summarized here cover both the innate and adaptative cellular immune response as well as strategies to overcome them and co...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/j.trim.2008.10.006
更新日期：2009-06-01 00:00:00
abstract：:We suggest that surveillance for squamous cell cancer might be particularly useful for transplant patients receiving statin therapy. We recommend that development of non melanoma skin cancer should be specified as a secondary endpoint in future large endpoint statin trials, and suggest that in transplant patients the ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2010.06.001
更新日期：2010-08-01 00:00:00
abstract：OBJECTIVE:To investigate the expression and significance of Sirt1 in renal allografts at the early stage of chronic renal allograft dysfunction (CRAD). METHODS:CRAD rat models were established using classical orthotopic F344-Lewis kidney transplantation. F344 and Lewis uninephrectomized rats were used as controls. Twe...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2018.02.006
更新日期：2018-06-01 00:00:00
abstract：:Diphtheria toxin (DT)-based anti-CD3 immunotoxins have clinical relevance in numerous applications including autoimmune disease therapies and organ transplantation tolerance protocols. Pre-existing anti-DT antibodies acquired either by vaccination against diphtheria toxin or infections with C. diphtheriae may interfer...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2012.05.003
更新日期：2012-08-01 00:00:00
abstract：:Alloreactivity probably involves T cell recognition of peptides bound to allogeneic HLA molecules. In order to study whether T cells recognizing common viral antigens presented by self HLA molecules also recognize some allogeneic HLA molecules we generated T lymphocyte clones (TLC) specific for herpes simplex virus (H...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(93)90005-s
更新日期：1993-01-01 00:00:00
abstract：:Complex polygenic and multifactorial diseases remain a challenge for human geneticists. Here we aim to remind basic definitions of multifactorial diseases and the genetic related concepts underlying classical methods. Knowledge on pathophysiological process and the genetic information available conditions the design o...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/j.trim.2005.03.015
更新日期：2005-08-01 00:00:00
abstract：:Clinical ophthalmologists are highly successful in grafting allogeneic corneas onto healthy eye graft beds--only a small percentage are rejected. By contrast, a high percentage of allografts are rejected when grafted into vascularized or '"high-risk' recipient eyes. The following experiments test the hypothesis that d...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(96)80034-7
更新日期：1996-03-01 00:00:00
abstract：:Despite improvements in the prevention and treatment of graft-versus-host disease (GvHD) this allogeneic immune response is still one of major complications following allogeneic stem cell transplantation (SCT). Identification of patients at risk for the development of acute and chronic GvHD would facilitate early inte...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2012.07.003
更新日期：2012-12-01 00:00:00
abstract：BACKGROUND:The transcription factor Forkhead Box P3 (FoxP3) is a marker of regulatory T cells (Tregs) - a subset of T cells known to suppress a wide range of immune responses. These cells are considered to be pivotal for the induction of tolerance to donor antigens in human allografts. We aimed to correlate the number ...
journal_title：Transplant immunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.trim.2013.08.002
更新日期：2013-12-01 00:00:00
abstract：:Persistent rejection in the face of treatment and multiple episodes of rejection are associated with the development of chronic rejection and graft loss in solid organ transplantation. The factors that create an environment for rejection that persists in the face of treatment are as yet not understood. The objective o...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2004.11.001
更新日期：2005-03-01 00:00:00
abstract：:Allospecific memory T cells are a recognized threat to the maintenance of solid-organ transplants. Limited information exists regarding the development of alloreactive memory T cells when post-transplant immunosuppression is present. The clinical practice of delaying calcineurin inhibitor (CNI) initiation post-transpl...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2014.10.001
更新日期：2015-01-01 00:00:00
abstract：:Using specific cell surface receptors lymphocytes continuously sample their environment. Maturation of the immune system and initiation of a specific immune response rely on an array of extracellular cues that elicit complex intracellular biochemical signals. Essential molecules involved in signal transduction from im...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/s0966-3274(02)00009-6
更新日期：2002-05-01 00:00:00
abstract：BACKGROUND:While acute neurotoxic side effects of calcineurin inhibitors (CNI) are well-known, data upon long-term effects on brain structure and function are sparse. We hypothesize that long-term CNI therapy affects the neuroimmune system, thereby, increasing the risk of neurodegeneration. Here, we measured the impact...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2019.101248
更新日期：2020-02-01 00:00:00
abstract：:B(io) B(reedding)/O(ttawa) K(alsburg) rats spontaneously develop insulin-dependent type 1 diabetes. Days before BB/OK rats become diabetic, their body seems to be flabby which may be attributed to loss of subcutaneous fat. However, the rats are normoglycemic and manifest 3-4 days later. This observation prompted us to...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2011.01.003
更新日期：2011-05-01 00:00:00
abstract：:Upon activation, T cells express CD40L, a member of the TNF cytokine superfamily, which serves as a ligand for CD40 on antigen presenting cells, including dendritic cells, B cells, and macrophages. While initial studies on the function of CD40 focused its role in the regulation of B cell activation, more recent studie...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(00)00026-5
更新日期：2000-11-01 00:00:00
abstract：:The expression of CD30 is restricted to cells of the immune system and strictly regulated under physiological conditions. However, active immune cells express CD30 and soluble CD30 (sCD30) is released. Several investigators reported the relevance for sCD30 as a predictive marker for allograft rejection following organ...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2010.07.001
更新日期：2010-08-01 00:00:00
abstract：BACKGROUND:So far, the ImmuKnow Immune Cell Function Assay (Cylex, Inc., Columbia, MD, USA) has been used to assess risks of infection and rejection in transplant patients. We hypothesized that the ImmuKnow assay might be used for mortality screening in transplant patients overall. METHODS:In the period of February 20...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2010.12.005
更新日期：2011-05-01 00:00:00
abstract：BACKGROUND:The importance of apoptosis contra necrosis for ischemia/reperfusion (RP) and acute rejection in concordant rodent xenotransplantation is largely unknown. We explored this question by comparing rodent allo and concordant xenotransplants with different morphological methods to detect apoptosis and biochemical...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/S0966-3274(03)00018-2
更新日期：2003-10-01 00:00:00
abstract：:Transplant rejection is a multifactorial process involving complex interactions between components of the innate and the acquired immune system. In view of the shortage of donor organs available for transplantation, xenotransplantation of pig organs into man has been considered as a potential solution. However, in com...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/s0966-3274(02)00042-4
更新日期：2002-05-01 00:00:00
abstract：BACKGROUND:Brain death impairs donor organ quality and accelerates immune responses after transplantation. Detailed aspects of immune activation following brain death remain unclear. We have established a mouse model and investigated the immediate consequences of brain death and anesthesia on immune responses. METHODS...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2012.04.002
更新日期：2012-08-01 00:00:00
abstract：BACKGROUND:Peripheral blood CD4+ T cell adenosine triphosphate (ATP) release has been reported to be an adjunct tool to evaluate global cellular immune response in solid-organ transplant recipients. However, the correlation between the ATP level and rejection was controversial. The aim of this prospective clinical stud...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2013.10.008
更新日期：2014-01-01 00:00:00
abstract：:Naturally occurring antibodies to the galactose alpha1,3 galactose (alpha gal) epitope expressed on xenogeneic grafts are a major barrier to organ transplantation in humans. Porcine small intestinal submucosa (SIS) expresses the alpha gal epitope and is currently being used as a bioscaffold for tissue remodeling. To e...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/s0966-3274(01)00044-2
更新日期：2002-06-01 00:00:00
abstract：:HMG-CoA reductase inhibitors, or statins, are effective lipid lowering agents, extensively used in medical practice. Statins have never been shown to be involved in the immune response, although few clinical reports have suggested a better outcome of cardiac transplantation in patients under pravastatin therapy. Major...
journal_title：Transplant immunology
pub_type： 杂志文章,评审
doi：10.1016/s0966-3274(02)00030-8
更新日期：2002-05-01 00:00:00
abstract：:Human anti-pig antibodies were obtained by perfusing pig hearts (n = 4) and kidneys (n = 8) with human AB or O plasma followed by elution with 3 M NaSCN. The antibodies were screened against a panel of 132 synthetic carbohydrates conjugated to bovine serum albumin using an enzyme-linked immunoassay. An anti-immunoglob...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(93)90047-c
更新日期：1993-01-01 00:00:00
abstract：:A rapid and robust limiting dilution assay was developed to measure the frequency of donor-reactive, IL-2 (interleukin 2) producing, helper T lymphocytes in the peripheral T cell population of organ allograft recipients. The IL-2 bioassay was performed using two methodologies to assess the response of CTLL-2 indicator...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/0966-3274(94)90006-x
更新日期：1994-12-01 00:00:00
abstract：:Adoptive cell therapies involving cell manipulation to achieve tolerance are increasingly being studied in animal models and in human trials. We have demonstrated that the specific removal of allo-stimulated dividing cells (or "pruning") promotes long-term allograft survival across a major MHC mismatch in transplant m...
journal_title：Transplant immunology
pub_type： 杂志文章
doi：10.1016/j.trim.2014.03.002
更新日期：2014-05-01 00:00:00
