Abstract:
:Oestradiol and the selective oestrogen receptor modulator (SERM) raloxifene have been shown to ameliorate collagen-induced arthritis (CIA) in rats and in mice. One aim was to investigate if raloxifene exerts its anti-arthritic and anti-osteoporotic effects during the induction or effector phase of arthritis. A second aim was to analyse if raloxifene activates the oestrogen response element (ERE) to produce its immune-modulator effects. CIA or collagen-antibody-induced arthritis (CAIA) was induced in ovariectomized DBA/1-mice. CIA was used for evaluation of treatment during the induction, and CAIA for the effector phase of arthritis and osteoporosis development. Raloxifene, oestradiol or vehicle was administered 5 days/week. The clinical disease was evaluated continuously. Bone marrow density (BMD) was analysed with peripheral quantitative computer tomography, paws were collected for histological examination, and sera were analysed for markers of bone and cartilage turnover and proinflammatory cytokines. Transgenic luciferase (Luc)-ERE mice were immunized with collagen (CII), and after 10 days injected once with raloxifene, oestradiol or vehicle before termination. Spleens were analysed for luciferase activity to measure ERE activation. Treatment with oestradiol or raloxifene during the induction phase of CIA failed to affect arthritis. Raloxifene did not hamper disease activity in CAIA, whereas oestradiol delayed the onset and ameliorated the severity. Both raloxifene and oestradiol preserved BMD in CAIA. CII-immunization increased the oestradiol-induced ERE activation in spleen, and raloxifene activated the ERE at about 25% the intensity of oestradiol. Further experiments are needed to elucidate the exact mechanisms behind this finding.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Jochems C,Islander U,Erlandsson M,Engdahl C,Lagerquist M,Ohlsson C,Nandakumar KS,Holmdahl R,Carlsten Hdoi
10.1111/j.1365-2249.2011.04397.xsubject
Has Abstractpub_date
2011-07-01 00:00:00pages
121-9issue
1eissn
0009-9104issn
1365-2249journal_volume
165pub_type
杂志文章abstract::The relation between the duration of bacterial infection and circulating immune complexes (CIC) level was evaluated using the C1q binding assay in a group of patients with well defined clinical sepsis. Fifty-four patients with endocarditis and 35 with post-open heart surgery mediastinitis were prospectively studied ov...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-03-01 00:00:00
abstract::B-lymphocytes from healthy children and young adults who were seronegative for autoantibodies and B lymphocytes from umbilical cord blood of newborns were induced to secrete a variety of autoantibodies upon infection with Epstein-Barr virus. Such autoantibody-secreting clones were obtained from different lymphoid tiss...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1985-10-01 00:00:00
abstract::The aim of the study was to test whether normal rat epiphyseal chondrocytes react with monoclonal antibodies (MoAbs) detecting different markers of lymphoid cells. For this purpose, isolated chondrocyte and, for comparison, splenocyte cytosmears were exposed to a battery of different MoAbs followed by indirect immunop...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1989-03-01 00:00:00
abstract::Lymphocytotoxic activity has been found by previous investigators in multiple sclerosis (MS) sera. We confirmed the presence of this activity in MS sera using techniques designed to eliminate possible sources of erroneous conclusion not considered in previous studies. We further characterized this activity and found i...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1983-07-01 00:00:00
abstract::Characterization of cold reactive lymphocytotoxic antibodies present in sera from Thai adults with malaria revealed that the antibodies are predominantly 19S (IgM), directed against both autologous and allogeneic mononuclear cells, complement-dependent, present in titres ranging from 1:2 to 1:16, and exhibit greater l...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1983-02-01 00:00:00
abstract::Dipeptidyl peptidase IV (DPIV), a T cell enzyme, has been implicated in the regulation of lymphocyte proliferation in response to lectins and allogeneic cells. A sensitive fluorimetric assay has been established for the enzyme and used to investigate DPIV activity, kinetics and the subcellular localization in lymphocy...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1988-11-01 00:00:00
abstract::In this paper, we report the presence on Epstein-Barr virus-transformed lymphoblastoid cell lines on platelets and on fibroblasts of an HLA-B27-associated cell surface complex (antigenically related to some antigens of Klebsiella K43 and K21) which is identical to or cross-reactive with the determinant present on the ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1981-07-01 00:00:00
abstract::A proportion of human monocytes form long needles of fibrin on their surfaces when they are briefly exposed to human plasma. A number of coagulation factor deficient plasmas (II, V, VII, VIII, von Willebrand, IX, X, XI and XII) were tested for their ability to promote formation of this fibrin. Monocytes failed to make...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1983-09-01 00:00:00
abstract::The aim of this study was to investigate the role of IL-12 in patients with RA. IL-12 (p70) and its associated cytokines were measured in sera and synovial fluid (SF) using an enzyme-linked immunosorbent method. Seven American College of Rheumatology (ACR) core set measures as well as IL-12 levels were sequentially mo...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2000.01095.x
更新日期:2000-01-01 00:00:00
abstract::Dendritic cells (DC) are specialized in the presentation of antigens and the initiation of specific immune responses. They have been involved recently in supporting innate immunity by interacting with various innate lymphocytes, such as natural killer (NK), NK T or T cell receptor (TCR)-gammadelta cells. The functiona...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
doi:10.1111/j.1365-2249.2008.03624.x
更新日期:2008-05-01 00:00:00
abstract::The kinetics of efflux of calcium mobilized from intracellular stores following activation of human neutrophils with the synthetic chemotactic tripeptide, fMLP (1 microM), as well as that of the subsequent store-operated influx of this cation, has been measured by radiometric procedures using 45Ca. These procedures en...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1997.5051403.x
更新日期:1997-10-01 00:00:00
abstract::A number of studies done in the last 10 years demonstrate the importance of iron in regulating the expression of T lymphoid cell surface markers, in influencing the expansion of different T cell subsets and in affecting different immune cell functions in vitro. It has been argued that some of the results obtained coul...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
doi:
更新日期:1989-01-01 00:00:00
abstract::We report here the characterization and fine specificity of a human monoclonal IgM lambda reacting with double stranded DNA. The purified IgM and its Fab fragment bind to DNA in the Farr or Crithidia lucilae assays as well as in an immunoenzymatic test. In the latter case, binding was inhibited by native DNA, by DNA a...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1985-02-01 00:00:00
abstract::For 'the total population of patients with sepsis, sepsis syndrome or SIRS', the question of whether intravenous immune globulin (IVIG) reduces mortality is neither proved nor disproved. For the sepsis subgroups 'postoperative sepsis with a sepsis score more than 19' and 'endotoxaemic, early septic shock', a significa...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
doi:
更新日期:1996-05-01 00:00:00
abstract::Cell transfer experiments were carried out with phytohaemagglutinin-induced normal human T lymphocyte colonies after 2--10 days of primary colony growth. The cells gave a cloning efficiency of 15% after 2 days of incubation and this decreased to 0.2% with the progressive growth of the colonies. The primary colonies ha...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
abstract::The diagnosis of neonatal sepsis is difficult, resulting in unnecessary treatment to minimize morbidity and mortality. We hypothesized that exposure to antenatal risk factors for sepsis alters the perinatal neutrophil phenotype. The study setting was a tertiary referral university-affiliated maternity and neonatal hos...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2007.03540.x
更新日期:2008-02-01 00:00:00
abstract::Human red cells (RBC) coated with IgG anti-D are cleared from the circulation to the spleen by macrophages which express IgG receptors (Fcgamma R). Polymorphisms of Fcgamma RIIa and Fcgamma RIIIa affect IgG binding in vitro, and may alter the efficiency of clearance of immune complexes in vivo. In a RBC clearance stud...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2003.02119.x
更新日期:2003-04-01 00:00:00
abstract::Hypersensitivity to Chironomidae (non-biting midges) has been a problem in Northern Sudan since about 1927 and is probably due to the working of dams which have produced lake-like conditions on parts of the Blue and main Niles where breeding has evidently increased. Studies were undertaken to determine whether this hy...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1978-10-01 00:00:00
abstract::Oral immunization with 25 mg/day of ovalbumin resulted in a number of changes in non-specific immune responsiveness, including increases in antibody produced to T-dependent but not T-independent antigens and the abrogation of tolerance induced by both oral and intravenous routes. The effect was transient, sex- and str...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1983-05-01 00:00:00
abstract::We report a study on immunoregulation of in vitro antibody to hepatitis B surface antigen (anti-HBs) synthesis induced by pokeweed mitogen (PWM) from peripheral blood mononuclear cells (PBMC) in chronic hepatitis B surface antigen (HBsAg) carriers and in 'high responders', (anti-HBs RIA ratio greater than or equal to ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1985-05-01 00:00:00
abstract::Fluorescent probes can monitor events in lymphocytes stimulated by mitogens and antigens. Early activation is associated with conformational changes in membrane macromolecules, and has been studied by measurement of fluorescence intensity or polarization of the membrane-localizing probes ANS, NPN, DPH and TMRITC. Subs...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
doi:
更新日期:1980-01-01 00:00:00
abstract::Mixed cryoglobulinaemia (MC) is a disorder characterized by the presence of large amounts of cryoprecipitating IgM-IgG complexes. An immune complex glomerulonephritis develops in one third of all patients, but its occurrence does not seem related to the amount of cryoglobulins in the sera, nor to their complement-fixi...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1994.tb06560.x
更新日期:1994-05-01 00:00:00
abstract::It has been suggested that the immune system may be responsible for the destruction of insulin secreting cells in some types of diabetes. In order to test this hypothesis, we studied the consequences of immune-mediated reactions on the function of pancreatic islet cells in vitro. A model was set up in vitro where mous...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1984-03-01 00:00:00
abstract::We have studied immunological, serological and clinical abnormalities in 264 HIV-positive and HIV-negative drug abusers. Ninety percent of the 264 drug addicts (mean age 26 +/- 0.8 years) were found to have HIV antibodies and there was a significant increase (P less than 0.01) in the frequency of HIV antibody positivi...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1988-05-01 00:00:00
abstract::Rheumatoid arthritis (RA), seronegative spondyloarthropathy (SA) and osteoarthritis (OA) patients receiving no steroid or disease-modifying therapy have been monitored, along with healthy controls, for their prevailing level of Epstein-Barr virus (EBV) infection using four independent indices of the EBV-host balance, ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1986-05-01 00:00:00
abstract::Environmental factors such as diet are known to play important roles in inflammatory bowel disease (IBD). Epidemiological studies have indicated that a high-fat diet is a risk factor for IBD. In addition, the balance between effector T cells (T(eff)) and regulatory T cells (T(reg)) contributes to the pathogenesis of m...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2007.03530.x
更新日期:2008-01-01 00:00:00
abstract::A solid-phase radioimmunoassay was developed to detect antibodies to liver membrane antigens in sera of patients with HBsAg-negative and -positive liver diseases and primary non-hepatic autoimmune diseases. Ten of fourteen patients with HBsAg-negative CAH had autoantibodies detected by RIA; negative results were obtai...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1980-11-01 00:00:00
abstract::We have shown previously that IgG2a anti-Thy-1 MoAb (ER4G) induces apoptosis of rat mesangial cells (GMC) in vitro. Since the classical complement pathway plays an essential role in Thy-1 nephritis, we analysed whether C1q, a subunit of the first component of complement, enhances the ER4G-mediated apoptosis of rat GMC...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1997.4741372.x
更新日期:1997-09-01 00:00:00
abstract::In susceptible mice, the heavy metal ion mercury is able to induce a strong immune activation, which resembles a T helper 2 (Th2) type of immune response and is characterized by a polyclonal B cell activation, formation of high levels of IgG1 and IgE antibodies, production of autoantibodies of different specificities ...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2001.01580.x
更新日期:2001-08-01 00:00:00
abstract::Maintenance of lung homeostasis involves a complex interaction between T lymphocytes and alveolar macrophages (AM), in which AM suppress pulmonary T cell proliferation to antigenic stimuli. To assess whether AM-mediated suppression is attenuated in healthy young infants, AM and peripheral blood mononuclear cells (PBMC...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2002.01848.x
更新日期:2002-05-01 00:00:00