Immunological methods for nursing research: from cells to systems.

Abstract:

:Scientists and clinicians frequently use immunological methods (IMs) to investigate complex biological phenomena. Commonly used IMs include immunocytochemistry (IC), enzyme-linked immunosorbent assays (ELISA) and flow cytometry. Each of these methodologies exploits a common principle in IMs -the binding of an antibody to its antigen. Scientists continue to develop new methodologies, such as high-throughput immunohistochemistry (IHC) and in vivo imaging techniques, which exploit antibody-antigen binding, to more accurately answer complex research questions involving single cells up to whole organ systems. The purpose of this paper is to discuss established and evolving IMs and to illustrate the application of these methods to nursing research.

journal_name

Biol Res Nurs

authors

Morrison HW,Downs CA

doi

10.1177/1099800411402494

subject

Has Abstract

pub_date

2011-07-01 00:00:00

pages

227-34

issue

3

eissn

1099-8004

issn

1552-4175

pii

1099800411402494

journal_volume

13

pub_type

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