Abstract:
AIM:The purpose of this paper is to review the techniques and implications of laser capture microdissection (LCM) to isolate tissue and DNA of interest using breast biopsy tissue as an example. BACKGROUND:Tissues are a heterogeneous mix of different cell types, and molecular alterations are often specific to a single cell type. An accurate correlation of molecular and morphologic pathologies requires the ability to procure pure populations of morphologically similar cells for molecular analysis. LCM is a technique for isolating highly pure cell populations of morphologically similar cells from a heterogeneous tissue section. METHOD:Nine invasive, paraffin-embedded breast biopsy specimens were obtained and analyzed. Depending on the size of the lesion, 500-1,000 shots using the 7.5- or 15-µm infrared laser beam were utilized to obtain an average of 2,000 cells. DNA was isolated from normal tissue and carcinomas and polymerase chain reaction (PCR) amplification was examined by agarose gel electrophoresis. The HER2/neu gene was amplified by standard PCR. A second round of PCR using nested primers to re-amplify the HER2/neu fragment was performed. RESULTS:Amplification of the HER2/neu gene with DNA isolated from pure cell populations by LCM was performed. The results indicated that 22% of the cases studied were positive for HER2/neu amplifications, which corresponds to the literature regarding HER2/neu amplification/overexpression. HER2/neu amplification could be detected as early as the ductal carcinoma in situ (DCIS) stage. CONCLUSION:LCM is an accurate and reliable method to acquire nucleic acid and protein profiles from a specific cell population in heterogeneous tissue.
journal_name
Biol Res Nursjournal_title
Biological research for nursingauthors
Zanni KL,Chan GKdoi
10.1177/1099800411402054subject
Has Abstractpub_date
2011-07-01 00:00:00pages
297-305issue
3eissn
1099-8004issn
1552-4175pii
1099800411402054journal_volume
13pub_type
杂志文章abstract::Central nervous system (CNS)-directed treatment for acute lymphoblastic leukemia, used to prevent disease recurrence in the brain, is essential for survival. Systemic and intrathecal methotrexate, commonly used for CNS-directed treatment, have been associated with cognitive problems during and after treatment. The cor...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800416644780
更新日期:2016-10-01 00:00:00
abstract:BACKGROUND:Insulin resistance plays a determinant role in the development of metabolic syndrome in adolescents. The objective of the present study was to determine the influence of factors commonly associated with insulin resistance in a sample of adolescents. METHODS:This cross-sectional study included 976 adolescent...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800416648207
更新日期:2016-10-01 00:00:00
abstract::Food intake and, subsequently, body weight are influenced by endogenous opioids acting in the central nervous system. Agonists for the opioid receptor increase food intake, whereas antagonists reduce food intake. Body weight, however, is the result of food consumed and energy expended. Although much has been reported ...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800406298774
更新日期:2007-04-01 00:00:00
abstract:BACKGROUND:Application of a thermal stimulus is a common procedure used to promote venodilation for peripheral intravenous cannulation (PIVC); however, the effects of thermal stimulation on the duration of venodilation and skin temperature of the forearm are unclear. AIM:To investigate the duration of venodilation ind...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800416651145
更新日期:2017-03-01 00:00:00
abstract:PURPOSE:In recent years, researchers have noted an "obesity paradox," where individuals with obesity survive sepsis at higher rates than their nonobese counterparts. This systematic review summarizes the literature on studies examining the association between obesity and 1-year mortality among patients admitted with se...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800419876070
更新日期:2020-01-01 00:00:00
abstract::Sleep disturbance is a common and disturbing symptom in military personnel, with many individuals progressing to the development of insomnia, which is characterized by increased arousals, wakefulness after sleep onset, and distorted sleep architecture. The molecular mechanisms underlying insomnia remain elusive, limit...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800415575343
更新日期:2015-07-01 00:00:00
abstract::Bidirectional communication between the immune system and the brain and the implications of this communication are emerging concepts in pain research. Although representing a small portion of the disc degeneration syndromes, lumbar herniated discs can cause significant symptoms that may persist even after surgical int...
journal_title:Biological research for nursing
pub_type: 杂志文章,评审
doi:10.1177/1099800404272221
更新日期:2005-01-01 00:00:00
abstract::The theoretical constructs of allostasis and allostatic load (AL) have contributed to our understanding of how constantly changing social and environmental factors impact physiological functioning and shape health and aging disparities, particularly along socioeconomic, gendered, racial, and ethnic lines. AL represent...
journal_title:Biological research for nursing
pub_type: 杂志文章,评审
doi:10.1177/1099800412455688
更新日期:2012-10-01 00:00:00
abstract:OBJECTIVE:The Finnmarksløpet dogsled race lasts up to 7 days. Helpers, who keep time, coach, and transport equipment, have to be alert though they have little and fragmented sleep. This study investigated disruptions of the rest-activity rhythm among helpers. METHODS:10 helpers were monitored by actigraph a week befor...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800412475077
更新日期:2014-04-01 00:00:00
abstract::The purposes of this quasi-experimental pilot study were to determine adherence to a 12-week group-based moderate-intensity walking program for sedentary adult outpatients with serious and persistent mental illness and to examine change from baseline to after the walking program in health status (mental and physical h...
journal_title:Biological research for nursing
pub_type: 临床试验,杂志文章
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更新日期:2005-10-01 00:00:00
abstract::Postpartum depression (PPD) is a serious mood disorder that may carry life-long consequences for a woman and her family. Multiple risk factors for PPD have been identified, including psychosocial, situational, and biological stimuli, several of which are experienced by most, if not all, postpartum women. Given the com...
journal_title:Biological research for nursing
pub_type: 杂志文章,评审
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更新日期:2010-07-01 00:00:00
abstract:BACKGROUND:Our program of research focuses on thermal and circulatory stability in extremely premature infants. In prior studies, we found that infants have long periods of time in which foot temperature (FT) is higher than central temperature. We thus wanted to determine whether blood flow in the foot is increased whe...
journal_title:Biological research for nursing
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更新日期:2017-01-01 00:00:00
abstract::Scientists and clinicians frequently use immunological methods (IMs) to investigate complex biological phenomena. Commonly used IMs include immunocytochemistry (IC), enzyme-linked immunosorbent assays (ELISA) and flow cytometry. Each of these methodologies exploits a common principle in IMs -the binding of an antibody...
journal_title:Biological research for nursing
pub_type: 杂志文章,评审
doi:10.1177/1099800411402494
更新日期:2011-07-01 00:00:00
abstract::To examine the effects of exercise and vitamin E supplementation on oxidative stress in older adults, 59 participants, age 76.3 +/- 4.2 years, were randomly assigned to 1 of 4 groups: an exercise group taking placebos (EGP) or vitamin E (EGE) or a sedentary group taking placebos (SGP) or vitamin E (SGE). Measures incl...
journal_title:Biological research for nursing
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2003-07-01 00:00:00
abstract::The purpose of this study was to examine whether the hepatitis B seroconversion rates in a small sample of subjects reflected that of the published rates, regardless of HIV status, after a hepatitis B vaccination series was completed while following strict vaccine protocols. The study, which had a nonexperimental, cor...
journal_title:Biological research for nursing
pub_type: 杂志文章
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更新日期:2015-01-01 00:00:00
abstract:OBJECTIVES:The objectives of this study were to determine the change in gene expression between two time points following stroke and to identify biomarkers of stroke recovery through gene expression profiling and pathway analysis. METHODS:Peripheral blood was collected from 34 ischemic stroke patients (confirmed by ma...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800414546492
更新日期:2015-05-01 00:00:00
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journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800403260693
更新日期:2004-01-01 00:00:00
abstract:OBJECTIVE:To investigate associations between components of cumulative risk (CR) and physiological stress responses in African American adolescents and evaluate emotion regulation as a mediator and sex as a moderator of these associations. METHODS:Cortisol and salivary alpha amylase (sAA) were collected in adolescents...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800417702742
更新日期:2017-07-01 00:00:00
abstract:PURPOSE:Activity restriction (AR), one of the most common interventions used in high-risk pregnancies, may exacerbate loss of bone mass. The purpose of this study was to determine changes over time in bone resorption in hospitalized AR women during late pregnancy. METHODS:This was a short-term prospective study conduc...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800412463120
更新日期:2014-01-01 00:00:00
abstract::Interpretation of changes in health and health care utilization patterns across the life span depends on an understanding of the effects of age, period, and cohort. The purpose of this article is to illustrate differences among three generations of women in demographic factors, health risk factors, and health status i...
journal_title:Biological research for nursing
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journal_title:Biological research for nursing
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更新日期:2018-01-01 00:00:00
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journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800405275202
更新日期:2005-07-01 00:00:00
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journal_title:Biological research for nursing
pub_type: 杂志文章
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更新日期:2018-03-01 00:00:00
abstract::The integration of molecular genetics approaches into the study of complex health phenomena is an increasingly important and available strategy for researchers across the health science disciplines. Pain sensation and response to painful stimuli are examples of complex health phenomena that are particularly amenable t...
journal_title:Biological research for nursing
pub_type: 杂志文章,评审
doi:10.1177/1099800406289909
更新日期:2006-07-01 00:00:00
abstract::The psychophysiological phenomenon of pain is of tremendous concern to nurses because of its potential to adversely affect the mental, emotional, and physical health of patients. Increasingly appreciated is the ability of pain to influence immune variables including enumerative and functional measures of leukocyte sub...
journal_title:Biological research for nursing
pub_type: 杂志文章,评审
doi:10.1177/1099800405286071
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND:There are documented associations between elevated maternal corticotropin-releasing hormone (CRH) levels and adverse pregnancy outcomes. However, reports of these findings often lack sufficient detail and rationale regarding the bioassay methodology. This shortcoming can be problematic for researchers who do...
journal_title:Biological research for nursing
pub_type: 杂志文章,评审
doi:10.1177/1099800408320970
更新日期:2008-07-01 00:00:00
abstract:OBJECTIVE:This study examined adherence to a personalized, community-based exercise intervention by sedentary adolescents with type 1 or type 2 diabetes or those with obesity. RESEARCH DESIGN AND METHODS:We conducted a pretest-posttest investigation to explore the application of an individualized exercise prescription...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800413500064
更新日期:2014-01-01 00:00:00
abstract::Listening to soothing music has been used as a complementary therapy to improve sleep quality. However, there is no empirical evidence for the effects of music videos (MVs) on sleep quality in adults with insomnia as assessed by polysomnography (PSG). In this randomized crossover controlled trial, we compared the effe...
journal_title:Biological research for nursing
pub_type: 杂志文章,随机对照试验
doi:10.1177/1099800414549237
更新日期:2015-05-01 00:00:00
abstract::The primary purpose of this exploratory study was to compare percentages of natural killer (NK) cells and activated NK and T cells, and both cytotoxic and in vitro cytokine production activity in women with and without symptomatic irritable bowel syndrome (IBS). A secondary purpose was to examine the relationships of ...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800402004001005
更新日期:2002-07-01 00:00:00
abstract::Cytokines such as angiogenin (ANG) and interleukin (IL-8) have been shown to be related to depressive symptoms and inflammatory diseases like coronary heart disease. They may thus be used as stress biomarkers to identify and prevent health problems. To investigate the relationship between cytokines and nurses' job-rel...
journal_title:Biological research for nursing
pub_type: 杂志文章
doi:10.1177/1099800408323219
更新日期:2008-10-01 00:00:00