Novel picornavirus in Turkey poults with hepatitis, California, USA.

Abstract:

:To identify a candidate etiologic agent for turkey viral hepatitis, we analyzed samples from diseased turkey poults from 8 commercial flocks in California, USA, that were collected during 2008-2010. High-throughput pyrosequencing of RNA from livers of poults with turkey viral hepatitis (TVH) revealed picornavirus sequences. Subsequent cloning of the ≈9-kb genome showed an organization similar to that of picornaviruses with conservation of motifs within the P1, P2, and P3 genome regions, but also unique features, including a 1.2-kb sequence of unknown function at the junction of P1 and P2 regions. Real-time PCR confirmed viral RNA in liver, bile, intestine, serum, and cloacal swab specimens from diseased poults. Analysis of liver by in situ hybridization with viral probes and immunohistochemical testing of serum demonstrated viral nucleic acid and protein in livers of diseased poults. Molecular, anatomic, and immunologic evidence suggests that TVH is caused by a novel picornavirus, tentatively named turkey hepatitis virus.

journal_name

Emerg Infect Dis

authors

Honkavuori KS,Shivaprasad HL,Briese T,Street C,Hirschberg DL,Hutchison SK,Lipkin WI

doi

10.3201/eid1703.101410

subject

Has Abstract

pub_date

2011-03-01 00:00:00

pages

480-7

issue

3

eissn

1080-6040

issn

1080-6059

journal_volume

17

pub_type

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