Suppression of the progression phenotype in somatic cell hybrids occurs in the absence of altered adenovirus type 5 gene expression.

abstract：:The general transcription factor TFIID is a multisubunit complex of TATA-binding protein (TBP) and 14 distinct TBP-associated factors (TAFs). Although TFIID constituents are required for transcription initiation of most mRNA encoding genes, the mechanism of TFIID action remains unclear. To gain insight into TFIID func...

journal_title：Molecular and cellular biology

authors： Sanders SL,Jennings J,Canutescu A,Link AJ,Weil PA

更新日期：2002-07-01 00:00:00

abstract：:Insulin-like growth factor II (IGF-II) is a major fetal growth factor. The IGF-II gene generates multiple mRNAs with different 5' untranslated regions (5' UTRs) that are translated in a differential manner during development. We have identified a human family of three IGF-II mRNA-binding proteins (IMPs) that exhibit m...

journal_title：Molecular and cellular biology

authors： Nielsen J,Christiansen J,Lykke-Andersen J,Johnsen AH,Wewer UM,Nielsen FC

更新日期：1999-02-01 00:00:00

abstract：:Sp1-like proteins are defined by three highly homologous C(2)H(2) zinc finger motifs that bind GC-rich sequences found in the promoters of a large number of genes essential for mammalian cell homeostasis. Here we report that TIEG2, a transforming growth factor beta-inducible Sp1-like protein with antiproliferative fun...

journal_title：Molecular and cellular biology

authors： Zhang JS,Moncrieffe MC,Kaczynski J,Ellenrieder V,Prendergast FG,Urrutia R

更新日期：2001-08-01 00:00:00

abstract：:Miz1 is a member of the POZ domain/zinc finger transcription factor family. In vivo, Miz1 forms a complex with the Myc oncoprotein and recruits Myc to core promoter elements. Myc represses transcription through Miz1 binding sites. We now show that the Miz1 gene is ubiquitously expressed during mouse embryogenesis. In ...

journal_title：Molecular and cellular biology

authors： Adhikary S,Peukert K,Karsunky H,Beuger V,Lutz W,Elsässer HP,Möröy T,Eilers M

更新日期：2003-11-01 00:00:00

abstract：:Type I interferons (IFNs) stimulate transcription through a latent heterotrimeric transcription factor composed of tyrosine-phosphorylated STAT1 and STAT2 and the DNA binding partner IRF9, with STAT2 contributing a critical transactivation domain. Human RVB1 and RVB2, which are highly conserved AAA(+) ATP binding prot...

journal_title：Molecular and cellular biology

authors： Gnatovskiy L,Mita P,Levy DE

更新日期：2013-10-01 00:00:00

abstract：:The regulatory subunits of cyclic AMP (cAMP)-dependent protein kinase from a dibutyryl cAMP-resistant S49 mouse lymphoma cell mutant, clone U200/65.1, and its revertants were visualized by two-dimensional polyacrylamide gel electrophoresis. Clone U200/65.1 co-expressed electrophoretically distinguishable mutant and wi...

journal_title：Molecular and cellular biology

authors： van Daalen Wetters T,Coffino P

更新日期：1983-02-01 00:00:00

abstract：:The Saccharomyces cerevisiae SWI4 gene encodes an essential transcription factor which controls gene expression at the G1/S transition of the cell cycle. SWI4 transcription itself is cell cycle regulated, and this periodicity is crucial for the normal cell cycle regulation of HO and at least two of the G1 cyclins. Sin...

journal_title：Molecular and cellular biology

authors： Foster R,Mikesell GE,Breeden L

更新日期：1993-06-01 00:00:00

abstract：:In the budding yeast Saccharomyces cerevisiae, mutations in the essential gene CDC1 cause defects in Golgi inheritance and actin polarization. However, the biochemical function of Cdc1p is unknown. Previous work showed that cdc1 mutants accumulate intracellular Ca(2+) and display enhanced sensitivity to the extracellu...

journal_title：Molecular and cellular biology

authors： Losev E,Papanikou E,Rossanese OW,Glick BS

更新日期：2008-05-01 00:00:00

abstract：:Mammalian cells were observed to die under conditions in which nutrients were depleted and, simultaneously, macroautophagy was inhibited either genetically (by a small interfering RNA targeting Atg5, Atg6/Beclin 1-1, Atg10, or Atg12) or pharmacologically (by 3-methyladenine, hydroxychloroquine, bafilomycin A1, or mone...

journal_title：Molecular and cellular biology

authors： Boya P,González-Polo RA,Casares N,Perfettini JL,Dessen P,Larochette N,Métivier D,Meley D,Souquere S,Yoshimori T,Pierron G,Codogno P,Kroemer G

更新日期：2005-02-01 00:00:00

abstract：:RNA editing in higher-plant chloroplasts involves C-to-U conversions at specific sites. Although in vivo analyses have been performed, little is known about the biochemical aspects of chloroplast editing reactions. Here we improved our original in vitro system and devised a procedure for preparing active chloroplast e...

journal_title：Molecular and cellular biology

authors： Miyamoto T,Obokata J,Sugiura M

更新日期：2002-10-01 00:00:00

abstract：:The E2F1, -2, and -3 transcription factors are key downstream targets of the retinoblastoma protein (pRB) tumor suppressor that drive expression of proliferation-associated genes. Here we use mutant mouse strains to investigate E2F3's role in vivo. We show that E2F3 is essential for embryonic viability in the pure 129...

journal_title：Molecular and cellular biology

authors： Cloud JE,Rogers C,Reza TL,Ziebold U,Stone JR,Picard MH,Caron AM,Bronson RT,Lees JA

更新日期：2002-04-01 00:00:00

abstract：:Numerous nonribosomal trans-acting factors involved in pre-rRNA processing have been characterized, but few of them are specifically required for the last cytoplasmic steps of 18S rRNA maturation. We have recently demonstrated that Rrp10p/Rio1p is such a factor. By BLAST analysis, we identified the product of a previo...

journal_title：Molecular and cellular biology

authors： Vanrobays E,Gelugne JP,Gleizes PE,Caizergues-Ferrer M

更新日期：2003-03-01 00:00:00

abstract：:Signaling downstream of mechanistic target of rapamycin complexes 1 and 2 (mTORC1 and mTORC2) controls specific and distinct aspects of insulin action and nutrient homeostasis in an interconnected and as yet unclear way. Mice lacking the mTORC1 substrate S6 kinase 1 (S6K1) maintain proper glycemic control with a high-...

journal_title：Molecular and cellular biology

authors： Treins C,Alliouachene S,Hassouna R,Xie Y,Birnbaum MJ,Pende M

更新日期：2012-10-01 00:00:00

abstract：:The simian virus 40 72-base-pair repeats substituted for the polyomavirus enhancer, allowing replication and transcription in mouse 3T6 but not monkey CV-1 cells. A polyomavirus genome containing the entire simian virus 40 control region replicated at low levels in 3T6 and CV-1 cells; however, transcripts were detecte...

journal_title：Molecular and cellular biology

authors： Campbell BA,Villarreal LP

更新日期：1985-06-01 00:00:00

abstract：:Fusion proteins known to activate transcription in vivo were tested for the ability to stimulate transcription in vitro in a recently developed Saccharomyces cerevisiae RNA polymerase II transcription system. One fusion protein, whose activation domain was derived from the herpesvirus transcriptional activator VP16, g...

journal_title：Molecular and cellular biology

authors： Chasman DI,Leatherwood J,Carey M,Ptashne M,Kornberg RD

更新日期：1989-11-01 00:00:00

abstract：:The signal-transducing adaptor protein 2 (STAP-2) is a recently identified adaptor protein that contains a pleckstrin homology (PH) and Src homology 2 (SH2)-like domains, as well as a proline-rich domain in its C-terminal region. In previous studies, we demonstrated that STAP-2 binds to MyD88 and IKK-alpha or IKK-beta...

journal_title：Molecular and cellular biology

authors： Ikeda O,Sekine Y,Yasui T,Oritani K,Sugiyma K,Muromoto R,Ohbayashi N,Yoshimura A,Matsuda T

更新日期：2008-08-01 00:00:00

abstract：:The products of c-fos and c-jun (Fos and Jun) function in gene regulation by interacting with the AP-1 binding site. Here we have examined the contribution of Fos and Jun toward transcriptional activity by using Fos and Jun polypeptides purified from Escherichia coli. Fos contained a transcriptional activation domain ...

journal_title：Molecular and cellular biology

authors： Abate C,Luk D,Gagne E,Roeder RG,Curran T

更新日期：1990-10-01 00:00:00

abstract：:Chicken ovalbumin upstream promoter transcription factors (COUP-TFs) strongly inhibit transcriptional activation mediated by nuclear hormone receptors, including hepatocyte nuclear factor 4 (HNF-4). COUP-TFs repress HNF-4-dependent gene expression by competition with HNF-4 for common binding sites found in several reg...

journal_title：Molecular and cellular biology

authors： Ktistaki E,Talianidis I

更新日期：1997-05-01 00:00:00

abstract：:Heteroduplexes were prepared from two plasmids, pRH4-14/TK and pRH5-8/TK, containing different amber mutations in the neomycin resistance gene (Neor). The Neor gene was engineered to be expressed in both bacterial and mammalian cells. A functional Neor gene conferred kanamycin resistance to bacteria and resistance to ...

journal_title：Molecular and cellular biology

authors： Folger KR,Thomas K,Capecchi MR

更新日期：1985-01-01 00:00:00

abstract：:Eight strains of Chinese hamster ovary (CHO) cells having an assembly-defective beta-tubulin were found among revertants of strain Cmd 4, a mutant with a conditional lethal mutation in a beta-tubulin gene (F. Cabral, M. E. Sobel, and M. M. Gottesman, Cell 20:29-36, 1980). The altered beta-tubulins in these strains hav...

journal_title：Molecular and cellular biology

authors： Boggs B,Cabral F

更新日期：1987-08-01 00:00:00

abstract：:We find that during embryogenesis the expression of HMGN1, a nuclear protein that binds to nucleosomes and reduces the compaction of the chromatin fiber, is progressively down-regulated throughout the entire embryo, except in committed but continuously renewing cell types, such as the basal layer of the epithelium. In...

journal_title：Molecular and cellular biology

authors： Furusawa T,Lim JH,Catez F,Birger Y,Mackem S,Bustin M

更新日期：2006-01-01 00:00:00

abstract：:The NF-kappa B/Rel family of transcription factors participates in the control of a wide array of genes, including genes involved in embryonic development and regulation of immune, inflammation, and stress responses. In most cells, inhibitory I kappa B proteins sequester NF-kappa B/Rel in the cytoplasm. Cellular stimu...

journal_title：Molecular and cellular biology

authors： Antonsson A,Hughes K,Edin S,Grundström T

更新日期：2003-02-01 00:00:00

abstract：:RNA interference with one of the eight Caenorhabditis elegans linker histone genes triggers desilencing of a repetitive transgene and developmental defects in the hermaphrodite germ line. These characteristics are similar to the phenotype of the C. elegans Polycomb group genes mes-2, mes-3, mes-4, and mes-6 (M. A. Jed...

journal_title：Molecular and cellular biology

authors： Jedrusik MA,Schulze E

更新日期：2007-03-01 00:00:00

abstract：:We have identified multiple distinct splicing enhancer elements within protein-coding sequences of the constitutively spliced human beta-globin pre-mRNA. Each of these highly conserved sequences is sufficient to activate the splicing of a heterologous enhancer-dependent pre-mRNA. One of these enhancers is activated by...

journal_title：Molecular and cellular biology

authors： Schaal TD,Maniatis T

更新日期：1999-01-01 00:00:00

abstract：:Genomic DNA in higher eucaryotic cells is organized into a series of loops, each of which may be affixed at its base to the nuclear matrix via a specific matrix attachment region (MAR). In this report, we describe the distribution of MARs within the amplified dihydrofolate reductase (DHFR) domain (amplicon) in the met...

journal_title：Molecular and cellular biology

authors： Dijkwel PA,Hamlin JL

更新日期：1988-12-01 00:00:00

abstract：:Temperature-sensitive mutants defective in 60S ribosomal subunit protein L16 of Saccharomyces cerevisiae were isolated through hydroxylamine mutagenesis of the RPL16B gene and plasmid shuffling. Two heat-sensitive and two cold-sensitive isolates were characterized. The growth of the four mutants is inhibited at their ...

journal_title：Molecular and cellular biology

authors： Moritz M,Pulaski BA,Woolford JL Jr

更新日期：1991-11-01 00:00:00

abstract：:The exons, their boundaries, and approximately half of the intronic deoxyribonucleic acid of the rat serum albumin gene were sequenced. In addition to the 14 exons identified earlier by R-loop analysis, a small exon was detected between the "leader" exon (Z) and exon B. The leader exon encoded the 5'-untranslated port...

journal_title：Molecular and cellular biology

authors： Sargent TD,Jagodzinski LL,Yang M,Bonner J

更新日期：1981-10-01 00:00:00

abstract：:The LINE-1, or L1, family of interspersed repeated DNA constitutes roughly 10% of the mammalian genome. Its abundance is due to duplicative transposition via an RNA intermediate, L1-encoded proteins, and reverse transcription. Although, in principle, transposition may occur in any cell type, expression and transpositi...

journal_title：Molecular and cellular biology

authors： Branciforte D,Martin SL

更新日期：1994-04-01 00:00:00

abstract：:A novel pp90rsk Ser/Thr kinase (referred to as RSK3) was cloned from a human cDNA library. The RSK3 cDNA encodes a predicted 733-amino-acid protein with a unique N-terminal region containing a putative nuclear localization signal. RSK3 mRNA was widely expressed (but was predominant in lung and skeletal muscle). By usi...

journal_title：Molecular and cellular biology

authors： Zhao Y,Bjørbaek C,Weremowicz S,Morton CC,Moller DE

更新日期：1995-08-01 00:00:00

abstract：:We have analyzed the fine structure of DNA repair in Chinese hamster ovary (CHO) cells within the G1 and G2 phases of the cell cycle. Repair of inactive regions of the genome has been suggested to increase in the G2 phase of the cell cycle compared with other phases. However, detailed studies of DNA repair in the G2 p...

journal_title：Molecular and cellular biology

authors： Petersen LN,Orren DK,Bohr VA

更新日期：1995-07-01 00:00:00