Abstract：
BACKGROUND:Therapies targeting ERBB2 have shown success in the clinic. However, response is not determined solely by expression of ERBB2. Levels of ERBB3, its preferred heterodimerisation partner and ERBB ligands may also have a role. METHODS:We measured NRG1 expression by real-time quantitative RT-PCR and ERBB receptors by western blotting and immunohistochemistry in bladder tumours and cell lines. RESULTS:NRG1α and NRG1β showed significant coordinate expression. NRG1β was upregulated in 78% of cell lines. In tumours, there was a greater range of expression with a trend towards increased NRG1α with higher stage and grade. Increased expression of ERBB proteins was detected in 15% (EGFR), 20% (ERBB2), 41% (ERBB3) and 0% (ERBB4) of cell lines. High EGFR expression was detected in 28% of tumours, associated with grade and stage (P=0.05; P=0.04). Moderate or high expression of ERBB2 was detected in 22% and was associated with stage (P=0.025). Cytoplasmic ERBB3 was associated with high tumour grade (P=0.01) and with ERBB2 positivity. In cell lines, NRG1β expression was significantly inversely related to ERBB3, but this was not confirmed in tumours. CONCLUSION:There is a wide spectrum of NRG1 and ERBB receptor expression in bladder cancer. In advanced tumours, EGFR, ERBB2 and ERBB3 upregulation is common and there is a relationship between expression of ERBB2 and ERBB3 but not the NRG1 ligand.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Forster JA,Paul AB,Harnden P,Knowles MAdoi
10.1038/bjc.2011.39subject
Has Abstractpub_date
2011-03-29 00:00:00pages
1135-43issue
7eissn
0007-0920issn
1532-1827pii
bjc201139journal_volume
104pub_type
杂志文章abstract：:6-Mercaptopurine (MP)-sensitive and -resistant cell culture lines were used to further characterize the apparent ability of MP nucleotide derivatives to overcome resistance to the parent drug. 6-Mercaptopurine-9-beta-D-ribofuranoside 5'-monophosphate [MPRP], bis(6-mercaptopurine-9-beta-D-ribofuranoside)-5', 5"'-monoph...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1985.73
更新日期：1985-04-01 00:00:00
abstract：:Survival and proliferation of cell cultures from human anaplastic astrocytomas were shown to be enhanced by glucocorticoids with an optimal concentration of approximately 2.5 x 10(-5)M (10 micrograms/ml). The stimulation of proliferation was only observed in a clonal growth assay and was reversed as the size of indivi...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1980.161
更新日期：1980-06-01 00:00:00
abstract：:This study was undertaken to determine the prevalence of anti-cardiolipin antibodies (ACLAs) in patients with malignancy and to investigate a possible association of ACLAs with thromboembolic events in such patients. The study included 216 patients with solid and non-solid malignancies and an age-matched control group...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1995.353
更新日期：1995-08-01 00:00:00
abstract：BACKGROUND:Diet modulates inflammation and inflammatory markers have been associated with cancer outcomes. In the Women's Health Initiative, we investigated associations between a dietary inflammatory index (DII) and invasive breast cancer incidence and death. METHODS:The DII was calculated from a baseline food freque...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2016.98
更新日期：2016-05-24 00:00:00
abstract：BACKGROUND:8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is the most abundant marker of DNA damage and it reflects oxidative stress. Human 8-oxoguanine glycosylase (hOGG1) is a DNA-repair enzyme that participates in 8-oxodG removal. METHODS:hOGG1 protein expression was immunohistochemically studied in 96 patients with...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2011.518
更新日期：2012-01-17 00:00:00
abstract：:Cytochrome P450 3A is a drug-metabolising enzyme activity due to CYP3A4 and CYP3A5 gene products, that is involved in the inactivation of anticancer drugs. This study analyses the potential of cytochrome P450 3A enzyme in human colorectal cancer to impact anticancer therapy with drugs that are cytochrome P450 3A subst...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6600494
更新日期：2002-09-09 00:00:00
abstract：:A crude membrane fraction isolated from mouse tumour cells was treated with various chemicals. The effects on the immunogenicity of the membrane sample were tested in syngeneic mice for tumour protection, using a challenge dose of 10(5) viable tumour cells. Best protection was obtained after immunization of mice with ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1978.236
更新日期：1978-10-01 00:00:00
abstract：BACKGROUND:Little is known about the factors that drive metastasis formation in colorectal cancer (CRC). Here, we set out to identify genes and proteins in patients with colorectal liver metastases that correlate with early disease recurrence. Such factors may predict a propensity for metastasis in earlier stages of CR...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.489
更新日期：2013-09-17 00:00:00
abstract：:Carzelesin is a cyclopropylpyrroloindole analogue which acts as a DNA-sequence-specific alkylating agent. In this phase I study, Carzelesin was given as a 4-weekly 10 min i.v. infusion to 51 patients with advanced solid tumours. Patients received a median of two courses (range 1-5) at one of nine dose levels: 24, 48, ...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/sj.bjc.6690232
更新日期：1999-03-01 00:00:00
abstract：:In this population-based Norwegian cohort study (2.1 million children), the impact of birth and parental characteristics on the risk of neuroblastoma (178 cases) was evaluated. In children below the age of 18 months, there was an increased neuroblastoma risk among those with congenital malformations and suggestion of ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604646
更新日期：2008-10-07 00:00:00
abstract：:Our purpose was to determine the response rate and median and overall survival of gemcitabine as monotherapy versus gemcitabine plus irinotecan in advanced or metastatic pancreatic cancer. Patients with histologically or cytologically confirmed adenocarcinoma who were chemotherapy and radiotherapy naive were enrolled....
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1038/sj.bjc.6603301
更新日期：2006-09-04 00:00:00
abstract：:As part of an NHS Executive Trent regional initiative we considered the role and cost-effectiveness of high dose chemotherapy in the treatment of relapsed Hodgkin's disease and non-Hodgkin's lymphoma. The key trials and case series show an additional patient benefit of 0.8-1.1 life years over standard chemotherapy. We...
journal_title：British journal of cancer
pub_type： 杂志文章,meta分析
doi：10.1054/bjoc.1999.0880
更新日期：2000-01-01 00:00:00
abstract：:Sublines of a human myeloid leukaemia cell line, KBM-3, with increasing degrees of resistance to the antileukaemic agent 4'-(9-acridinlylamino) methanesulphon-m-anisidide (m-AMSA) were evaluated for their response to this drug using a clonogenic assay to measure cell survival and alkaline elution to assess m-AMSA indu...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1990.11
更新日期：1990-01-01 00:00:00
abstract：:5'-nucleotidase activity, arachidonate metabolism and adenosine uptake were measured in P388 murine leukaemia cells and in a subline resistant to doxorubicin. These membranes related activities were found to be increased in the doxorubicin resistant cell line, compared to the drug sensitive cells. It is suggested that...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1984.71
更新日期：1984-04-01 00:00:00
abstract：:Exposure to the fibrous mineral erionite is known to induce mesothelioma in man and laboratory animals. Previous studies demonstrated the presence of a trypsin-like protease associated with tumour cells. This protease could be demonstrated by the use of fluorescent probes which located cells possessing this enzyme. We...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1988.269
更新日期：1988-11-01 00:00:00
abstract：:Fifteen patients with acute myelogenous leukaemia were studied to determine if their remission blood leucocytes could be stimulated into taking up [3H] thymidine after in vitro culture with their own cryo-preserved irradiated AML leukaemia cells. In 6/15 patients it was possible to show autologous recognition and equa...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1977.39
更新日期：1977-03-01 00:00:00
abstract：:During the period from 1950 to 1952, three patients were studied by electrophoresis according to Tiselius on account of anticomplementary activity at WR; the presence of an M-component was demonstrated. On several later occasions it was observed that this component at first seemed to remain unchanged, later it was sli...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1971.53
更新日期：1971-09-01 00:00:00
abstract：:Hypomethylation has been reported to be responsible for the activation of several oncogenes. The possibility that hypomethylation is involved in the regulation of MET transcription was investigated through the analysis of the methylation status of one CpG island containing 43 CpGs in six cases of papillary carcinoma, ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6601988
更新日期：2004-08-16 00:00:00
abstract：BACKGROUND:Eukaryotic translation elongation factor 1A2 (eEF1A2) is a known proto-oncogene. We proposed that stimulation of the eEF1A2 expression in cancer tissues is caused by the loss of miRNA-mediated control. METHODS:Impact of miRNAs on eEF1A2 at the mRNA and protein levels was examined by qPCR and western blot, r...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.243
更新日期：2013-06-11 00:00:00
abstract：BACKGROUND:The Mediterranean diet has been shown to have a beneficial role on various neoplasms, but data are scanty on pancreatic cancer. METHODS:We analysed data from two case-control studies conducted in Italy between 1983 and 2008, including 362 and 326 pancreatic cancer cases and 1552 and 652 hospital-controls, r...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2013.345
更新日期：2013-09-03 00:00:00
abstract：:Radiographically occult bronchogenic squamous cell carcinomas are early lung cancers that localize mainly in the bronchial wall, and are thought to be a good model for investigating genetic alterations through lung cancer progression. In order to elucidate sequential genetic changes in lung cancers, we analysed the in...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1998.549
更新日期：1998-09-01 00:00:00
abstract：:The aim of this phase I study was to determine the maximum tolerated dose of a 3-h infusion of paclitaxel, combined with carboplatin at a fixed AUC of 7 mg ml-1 min every 4 weeks for up to six cycles and to evaluate any possible pharmacokinetic interaction. Twelve chemonaive patients with ovarian cancer were treated w...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章
doi：10.1038/bjc.1997.47
更新日期：1997-01-01 00:00:00
abstract：:This study evaluates the efficacy of capecitabine using data from a large, well-characterised population of patients with metastatic colorectal cancer (mCRC) treated in two identically designed phase III studies. A total of 1207 patients with previously untreated mCRC were randomised to either oral capecitabine (1250 ...
journal_title：British journal of cancer
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1038/sj.bjc.6601676
更新日期：2004-03-22 00:00:00
abstract：:RNA tumour virus genes are contained in the chromosomal DNA of most vertebrates, and may be transmitted vertically from parent to progeny along with other cellular genes, as well as horizontally as infectious particles. Activation of these viral genes may be part of the means by which RNA tumour viruses produce cancer...
journal_title：British journal of cancer
pub_type： 杂志文章,评审
doi：10.1038/bjc.1978.23
更新日期：1978-02-01 00:00:00
abstract：BACKGROUND:New therapeutic options for metastatic pancreatic cancer are urgently needed. In pancreatic cancer, overexpression of the epidermal growth factor receptor 2 (HER2) has been reported in up to 45%. This multicentre phase II study investigated the efficacy and toxicity of the HER2 antibody trastuzumab combined ...
journal_title：British journal of cancer
pub_type： 杂志文章,多中心研究
doi：10.1038/bjc.2012.18
更新日期：2012-03-13 00:00:00
abstract：BACKGROUND:We assessed the sensitivity to adjuvant chemotherapy in cell cycle checkpoint kinase 2 (CHEK2) vs non-CHEK2 breast cancer patients by comparing the contralateral breast cancer incidence and distant disease-free and breast cancer-specific survival between both groups, stratified for adjuvant chemotherapy. ME...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.2014.306
更新日期：2014-08-26 00:00:00
abstract：:In a clinical investigation of observed postoperative survival, 563 patients have been registered for primary surgical treatment of colorectal cancer since 1974. The potential prognostic factors examined within the first days of hospitalization for primary resection included age of the patients, operability, location ...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1981.250
更新日期：1981-11-01 00:00:00
abstract：:The cellular pharmacology of a series of C8-linked pyrrolobenzodiazepine dimers with polymethylene linkers of n = 3-6 (compounds 1-4) has been studied in a range of human tumour cell lines. The four compounds showed the same pattern of relative activity in five ovarian carcinoma cell lines and one cervical carcinoma c...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/bjc.1994.248
更新日期：1994-07-01 00:00:00
abstract：:Laminin-332 is major component of epithelial basement membrane, and has an important role in cell migration and tumour invasion. Recently, the phosphatidylinositol 3-kinase (PI3K) activation induced by laminin-332 during carcinogenesis or tumour invasion has been highlighted in skin squamous cell carcinoma. The expres...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6604252
更新日期：2008-03-11 00:00:00
abstract：:Angiotensin II, a main effector peptide in the renin-angiotensin system, acts as a growth-promoting and angiogenic factor via type 1 angiotensin II receptors (AT(1)R). We have recently demonstrated that angiotensin II enhanced tumour cell invasion and vascular endothelial growth factor (VEGF) secretion via AT1R in ova...
journal_title：British journal of cancer
pub_type： 杂志文章
doi：10.1038/sj.bjc.6602961
更新日期：2006-02-27 00:00:00
