The anterior chamber of the eye as a clinical transplantation site for the treatment of diabetes: a study in a baboon model of diabetes.


AIMS/HYPOTHESIS:The aim of this study was to provide evidence that the anterior chamber of the eye serves as a novel clinical islet implantation site. METHODS:In a preclinical model, allogeneic pancreatic islets were transplanted into the anterior chamber of the eye of a baboon model for diabetes, and metabolic and ophthalmological outcomes were assessed. RESULTS:Islets readily engrafted on the iris and there was a decrease in exogenous insulin requirements due to insulin secretion from the intraocular grafts. No major adverse effects on eye structure and function could be observed during the transplantation period. CONCLUSIONS/INTERPRETATION:Our study demonstrates the long-term survival and function of allogeneic islets after transplantation into the anterior chamber of the eye. The safety and simplicity of this procedure provides support for further studies aimed at translating this technology into the clinic.






Perez VL,Caicedo A,Berman DM,Arrieta E,Abdulreda MH,Rodriguez-Diaz R,Pileggi A,Hernandez E,Dubovy SR,Parel JM,Ricordi C,Kenyon NM,Kenyon NS,Berggren PO




Has Abstract


2011-05-01 00:00:00












  • Long-term effects of exposure of pancreatic islets to nicotinamide in vitro on DNA synthesis, metabolism and B-cell function.

    abstract::Using 3H-thymidine labeling techniques, we found that rates of DNA synthesis in islet cells doubled when mouse pancreatic islets were cultured for 1 week with 10 mmol/l nicotinamide, a potent poly(ADP-ribose) synthetase inhibitor. Culture with nicotinamide partially inhibited glucose-stimulated insulin release, wherea...


    pub_type: 杂志文章


    authors: Sandler S,Andersson A

    更新日期:1986-03-01 00:00:00

  • A missense mutation in the CD38 gene, a novel factor for insulin secretion: association with Type II diabetes mellitus in Japanese subjects and evidence of abnormal function when expressed in vitro.

    abstract::Cyclic adenosine 5'diphosphate-ribose (cADPR) is thought to have a second messenger role in insulin secretion through mobilisation of Ca2+. As human lymphocyte antigen CD38 has both ADP-ribosyl cyclase and cADPR hydrolase activity, it may be important in glucose-induced insulin secretion in islets. Thirty one randomly...


    pub_type: 杂志文章


    authors: Yagui K,Shimada F,Mimura M,Hashimoto N,Suzuki Y,Tokuyama Y,Nata K,Tohgo A,Ikehata F,Takasawa S,Okamoto H,Makino H,Saito Y,Kanatsuka A

    更新日期:1998-09-01 00:00:00

  • Long-term suppression of postprandial hyperglycaemia with acarbose retards the development of neuropathies in the BB/W-rat.

    abstract::The effect of the alpha-glucosidase inhibitor acarbose on postprandial hyperglycaemia was explored in the spontaneously diabetic BB/W-rat. Acarbose-treatment (5 body of diabetic BB/W-rats maintained on small doses of insulin, was associated with a 40% reduction in the 24-h glucose area compared t...


    pub_type: 杂志文章


    authors: Sima AA,Chakrabarti S

    更新日期:1992-04-01 00:00:00

  • In vitro kinetics of insulin release by microencapsulated rat islets: effect of the size of the microcapsules.

    abstract::Microencapsulation has been proposed to protect islets of Langerhans against immune rejection in xenogenic transplantation. However, to achieve glucose homeostasis in human diabetic patients, insulin release by microencapsulated islets must increase in response to a glucose load. We microencapsulated isolated rat isle...


    pub_type: 杂志文章


    authors: Chicheportiche D,Reach G

    更新日期:1988-01-01 00:00:00

  • Asymmetric dimethylarginine (ADMA): a potential link between endothelial dysfunction and cardiovascular diseases in insulin resistance syndrome?

    abstract::Endothelium-derived nitric oxide plays a major role in the regulation of vascular tone and in the maintenance of vascular homeostasis. Endothelial dysfunction with impaired nitric oxide biosynthesis and decreased bioavailability has been implicated in insulin resistance syndrome and Type II (non-insulin-dependent) dia...


    pub_type: 杂志文章,评审


    authors: Chan NN,Chan JC

    更新日期:2002-12-01 00:00:00

  • VAV2, a guanine nucleotide exchange factor for Rac1, regulates glucose-stimulated insulin secretion in pancreatic beta cells.

    abstract:AIMS/HYPOTHESIS:Rho GTPases (Ras-related C3 botulinum toxin substrate 1 [Rac1] and cell division cycle 42 [Cdc42]) have been shown to regulate glucose-stimulated insulin secretion (GSIS) via cytoskeletal remodelling, trafficking and fusion of insulin-secretory granules with the plasma membrane. GTP loading of these G p...


    pub_type: 杂志文章


    authors: Veluthakal R,Tunduguru R,Arora DK,Sidarala V,Syeda K,Vlaar CP,Thurmond DC,Kowluru A

    更新日期:2015-11-01 00:00:00

  • The Pro12Ala variant at the peroxisome proliferator-activated receptor gamma gene and change in obesity-related traits in the Diabetes Prevention Program.

    abstract:AIMS/HYPOTHESIS:Peroxisome proliferator-activated receptor gamma (PPARgamma), encoded by the PPARG gene, regulates insulin sensitivity and adipogenesis, and may bind polyunsaturated fatty acids (PUFA) and thiazolidinediones in a ligand-dependent manner. The PPARG proline for alanine substitution at position 12 (Pro12Al...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Franks PW,Jablonski KA,Delahanty L,Hanson RL,Kahn SE,Altshuler D,Knowler WC,Florez JC,Diabetes Prevention Program Research Group.

    更新日期:2007-12-01 00:00:00

  • Role of insulin resistance in the pathogenesis of NIDDM.

    abstract::The above discussion illustrating the multitude of variables which influence insulin sensitivity in normal subjects challenges the prevailing view that insulin sensitivity is genetically determined in patients with NIDDM. The lack of accurate quantitation of all determinants of insulin sensitivity in the cross-section...


    pub_type: 杂志文章,评审


    authors: Yki-Järvinen H

    更新日期:1995-12-01 00:00:00

  • B-type natriuretic peptide (BNP) affects the initial response to intravenous glucose: a randomised placebo-controlled cross-over study in healthy men.

    abstract:AIMS/HYPOTHESIS:B-type natriuretic peptide (BNP) is a hormone released from cardiomyocytes in response to cell stretching and elevated in heart failure. Recent observations indicate a distinct connection between chronic heart failure and diabetes mellitus. This study investigated the role of BNP on glucose metabolism. ...


    pub_type: 杂志文章,随机对照试验


    authors: Heinisch BB,Vila G,Resl M,Riedl M,Dieplinger B,Mueller T,Luger A,Pacini G,Clodi M

    更新日期:2012-05-01 00:00:00

  • Proteomic characterisation reveals active Wnt-signalling by human multipotent stromal cells as a key regulator of beta cell survival and proliferation.

    abstract:AIMS/HYPOTHESIS:Novel strategies to stimulate the expansion of beta cell mass in situ are warranted for diabetes therapy. The aim of this study was to elucidate the secretome of human bone marrow (BM)-derived multipotent stromal cells (MSCs) with documented islet regenerative paracrine function. We hypothesised that re...


    pub_type: 杂志文章


    authors: Kuljanin M,Bell GI,Sherman SE,Lajoie GA,Hess DA

    更新日期:2017-10-01 00:00:00

  • Diabetes mellitus and the incidence of hip fracture: results from the Nord-Trøndelag Health Survey.

    abstract:AIMS/HYPOTHESIS:To study if people with Type I (insulin-dependent) or Type II (non-insulin-dependent) diabetes mellitus have increased risk of hip fracture. METHODS:The study population consisted of 35,444 people 50 years of age and older, attending a health screening in a Norwegian county. They were followed up with ...


    pub_type: 杂志文章


    authors: Forsén L,Meyer HE,Midthjell K,Edna TH

    更新日期:1999-08-01 00:00:00

  • Engagement of the insulin-sensitive pathway in the stimulation of glucose transport by alpha-lipoic acid in 3T3-L1 adipocytes.

    abstract:AIMS/HYPOTHESIS:A natural cofactor of mitochondrial dehydrogenase complexes and a potent antioxidant, alpha-lipoic acid improves glucose metabolism in people with Type II (non-insulin-dependent) diabetes mellitus and in animal models of diabetes. In this study we investigated the cellular mechanism of action of alpha-l...


    pub_type: 杂志文章


    authors: Yaworsky K,Somwar R,Ramlal T,Tritschler HJ,Klip A

    更新日期:2000-03-01 00:00:00

  • A high prevalence of glucokinase mutations in gestational diabetic subjects selected by clinical criteria.

    abstract:AIMS/HYPOTHESIS:Patients with glucokinase mutations are characterised by mild, persistent fasting hyperglycaemia, a small increment in glucose in response to an oral load and a dominant family history. These patients frequently present with gestational diabetes and often require insulin treatment during pregnancy. We a...


    pub_type: 杂志文章


    authors: Ellard S,Beards F,Allen LI,Shepherd M,Ballantyne E,Harvey R,Hattersley AT

    更新日期:2000-02-01 00:00:00

  • Increased osteoclastic activity in acute Charcot's osteoarthropathy: the role of receptor activator of nuclear factor-kappaB ligand.

    abstract:AIMS/HYPOTHESIS:Our aims were to compare osteoclastic activity between patients with acute Charcot's osteoarthropathy and diabetic and healthy controls, and to determine the effect of the receptor activator of nuclear factor-kappaB ligand (RANKL) and its decoy receptor osteoprotegerin (OPG). METHODS:Peripheral blood m...


    pub_type: 杂志文章


    authors: Mabilleau G,Petrova NL,Edmonds ME,Sabokbar A

    更新日期:2008-06-01 00:00:00

  • A genetic marker at the glucokinase gene locus for type 2 (non-insulin-dependent) diabetes mellitus in Mauritian Creoles.

    abstract::The prevalence of Type 2 (non-insulin-dependent) diabetes mellitus is high in Mauritius, a multiethnic island nation in the southwestern Indian Ocean. Evaluation of candidate genes in the different ethnic groups represents a means of assessing the genetic component. As glucokinase is known to be a key regulator of glu...


    pub_type: 杂志文章


    authors: Chiu KC,Province MA,Dowse GK,Zimmet PZ,Wagner G,Serjeantson S,Permutt MA

    更新日期:1992-07-01 00:00:00

  • Troglitazone reduces plasminogen activator inhibitor-1 expression and secretion in cultured human adipocytes.

    abstract:AIMS/HYPOTHESIS:Increased plasma plasminogen activator inhibitor-1 (PAI-1) concentrations are characteristic for subjects with insulin resistance and could contribute to the increased cardiovascular risk in this state. In this study, we investigated the effect of troglitazone, a ligand of the nuclear receptor peroxisom...


    pub_type: 杂志文章


    authors: Gottschling-Zeller H,Röhrig K,Hauner H

    更新日期:2000-03-01 00:00:00

  • Dysregulation of glucose transport in hearts of genetically obese (fa/fa) rats.

    abstract::Overall D-glucose metabolism and 3-0-methylglucose transport were measured in the perfused heart preparation of lean and genetically obese (fa/fa) rats. Absolute values of basal and insulin-stimulated glucose metabolism were decreased in hearts of 15-week-old obese rats when compared to lean age-matched controls. Basa...


    pub_type: 杂志文章


    authors: Zaninetti D,Crettaz M,Jeanrenaud B

    更新日期:1983-12-01 00:00:00

  • Beta-cell proliferation in normal and streptozotocin-treated newborn rats: site, dynamics and capacity.

    abstract::Regeneration of neonatal beta cells after streptozotocin (STZ)-induced destruction may be due to either replication from pre-existing intra-islet beta cells or extra-islet precursor cells. To further investigate this issue, beta-cell growth was analysed in normal and streptozotocin-treated newborn rats (100 micrograms...


    pub_type: 杂志文章


    authors: Wang RN,Bouwens L,Klöppel G

    更新日期:1994-11-01 00:00:00

  • Alloxan-induced alteration of insulin release, rubidium efflux and glucose metabolism in rat islets stimulated by various secretagogues.

    abstract::Insulin release and 86Rb efflux were studied in perifused rat islets exposed in vitro to alloxan (2 mmol/l) for 5 min. At a low glucose concentration, alloxan transiently increased 86Rb efflux. Alloxan immediately and completely abolished the secretory response to glucose (15 mmol/l) and markedly delayed the reduction...


    pub_type: 杂志文章


    authors: Henquin JC,Malvaux P,Lambert AE

    更新日期:1979-04-01 00:00:00

  • Elevated GLUT 1 level in crude muscle membranes from diabetic Zucker rats despite a normal GLUT 1 level in perineurial sheaths.

    abstract::Recently, we demonstrated that approximately 60% of GLUT 1 in a crude membrane fraction of rat skeletal muscle originates from perineurial sheaths. To study the in vivo regulation of GLUT 1 expression in different tissues in muscles, we measured the level of GLUT 1 in crude muscle membranes and in perineurial sheaths ...


    pub_type: 杂志文章


    authors: Handberg A,Kayser L,Høyer PE,Micheelsen J,Vinten J

    更新日期:1994-05-01 00:00:00

  • Glucose-induced changes of multiple mouse islet proteins analysed by two-dimensional gel electrophoresis and mass spectrometry.

    abstract:AIMS/HYPOTHESIS:The aim of this study was to investigate molecular mechanisms of glucose-induced changes in islets of Langerhans by analysing global changes in protein patterns of islets exposed to elevated glucose concentrations. METHODS:Islets were isolated from C57BL/6J mice and used either directly or after exposu...


    pub_type: 杂志文章


    authors: Ahmed M,Bergsten P

    更新日期:2005-03-01 00:00:00

  • Birthweight and early-onset type 2 diabetes in American Indians: differential effects in adolescents and young adults and additive effects of genotype, BMI and maternal diabetes.

    abstract:AIMS/HYPOTHESIS:The aim of this work was to estimate the impact of birthweight on early-onset (age <40 years) type 2 diabetes. METHODS:A longitudinal study of American Indians, aged ≥5 years, was conducted from 1965 to 2007. Participants who had a recorded birthweight were followed until they developed diabetes or the...


    pub_type: 杂志文章


    authors: Olaiya MT,Wedekind LE,Hanson RL,Sinha M,Kobes S,Nelson RG,Baier LJ,Knowler WC

    更新日期:2019-09-01 00:00:00

  • Orosomucoid in urine predicts cardiovascular and over-all mortality in patients with Type II diabetes.

    abstract:AIMS/HYPOTHESIS:Urinary orosomucoid excretion rate is increased in a substantial proportion of patients with Type II (non-insulin-dependent) diabetes mellitus and normal urinary albumin excretion rate. The aim of this study was to determine whether increased urinary orosomucoid excretion rate is predictive of increased...


    pub_type: 杂志文章


    authors: Christiansen MS,Hommel E,Magid E,Feldt-Rasmussen B

    更新日期:2002-01-01 00:00:00

  • A novel role for vitamin D: modulation of expression and function of the local renin-angiotensin system in mouse pancreatic islets.

    abstract:AIMS/HYPOTHESIS:The aim of this study was to demonstrate that hormonal vitamin D (calcitriol) modulates the local pancreatic islet renin-angiotensin system (RAS) whilst improving islet beta cell secretory function. METHODS:Isolated islets cultured ex vivo under high- or low-glucose conditions and treated with or witho...


    pub_type: 杂志文章


    authors: Cheng Q,Li YC,Boucher BJ,Leung PS

    更新日期:2011-08-01 00:00:00

  • Engineered cell lines for insulin replacement in diabetes: current status and future prospects.

    abstract::The recently completed diabetes complications and control trial has highlighted the need for improvement of insulin delivery systems for treatment of insulin-dependent diabetes mellitus. Despite steady improvement in methods for islet and whole pancreas transplantation over the past three decades, the broad-scale appl...


    pub_type: 杂志文章,评审


    authors: Newgard CB,Clark S,BeltrandelRio H,Hohmeier HE,Quaade C,Normington K

    更新日期:1997-07-01 00:00:00

  • Saturated non-esterified fatty acids stimulate de novo diacylglycerol synthesis and protein kinase c activity in cultured aortic smooth muscle cells.

    abstract:AIMS/HYPOTHESIS:Insulin resistance is linked with a cluster of multiple risk factors and excessive acceleration of atherosclerosis. The underlying mechanism is not, however, fully understood. METHODS:To determine the link between insulin resistance and altered vascular function, we focused on the effect of various non...


    pub_type: 杂志文章


    authors: Yu HY,Inoguchi T,Kakimoto M,Nakashima N,Imamura M,Hashimoto T,Umeda F,Nawata H

    更新日期:2001-05-01 00:00:00

  • Genetic variation of the platelet- surface integrin GPIIb-IIIa (PIA1/A2-SNP) shows a high association with Type 2 diabetes mellitus.

    abstract:AIMS/HYPOTHESIS:The gene encoding the beta(3)-subunit (GPIIIa) of the platelet alpha(2)beta(3)-integrin (fibrinogen receptor) shows a polymorphism PlA1/A2 with the A2 allele putatively associated with an increased risk of acute ischaemic events. This study investigated whether Type 2 diabetes as a particular macrovascu...


    pub_type: 杂志文章


    authors: Tschoepe D,Menart B,Ferber P,Altmann C,Haude M,Haastert B,Roesen P

    更新日期:2003-07-01 00:00:00

  • Epidemiology of gestational diabetes mellitus according to IADPSG/WHO 2013 criteria among obese pregnant women in Europe.

    abstract:AIMS/HYPOTHESIS:Accurate prevalence estimates for gestational diabetes mellitus (GDM) among pregnant women in Europe are lacking owing to the use of a multitude of diagnostic criteria and screening strategies in both high-risk women and the general pregnant population. Our aims were to report important risk factors for...


    pub_type: 杂志文章


    authors: Egan AM,Vellinga A,Harreiter J,Simmons D,Desoye G,Corcoy R,Adelantado JM,Devlieger R,Van Assche A,Galjaard S,Damm P,Mathiesen ER,Jensen DM,Andersen L,Lapolla A,Dalfrà MG,Bertolotto A,Mantaj U,Wender-Ozegowska E,Zawi

    更新日期:2017-10-01 00:00:00

  • Evidence for a placento-insular axis in the rat fetus.

    abstract::Rat pups delivered by caesarian section one day before term, either remained attached to (experimental group) or were separated from their placenta (control group). Both groups were transferred into an incubator and their metabolic parameters studied. In the control group, plasma amino acid concentration fell from 11....


    pub_type: 杂志文章


    authors: Sodoyez-Goffaux F,Sodoyez JC,De Vos CJ

    更新日期:1981-05-01 00:00:00

  • Beta cell compensation for insulin resistance in Zucker fatty rats: increased lipolysis and fatty acid signalling.

    abstract:AIMS/HYPOTHESIS:The aim of this study was to determine the role of fatty acid signalling in islet beta cell compensation for insulin resistance in the Zucker fatty fa/fa (ZF) rat, a genetic model of severe obesity, hyperlipidaemia and insulin resistance that does not develop diabetes. MATERIALS AND METHODS:NEFA augmen...


    pub_type: 杂志文章


    authors: Nolan CJ,Leahy JL,Delghingaro-Augusto V,Moibi J,Soni K,Peyot ML,Fortier M,Guay C,Lamontagne J,Barbeau A,Przybytkowski E,Joly E,Masiello P,Wang S,Mitchell GA,Prentki M

    更新日期:2006-09-01 00:00:00