Expansion of human trophoblastic spheroids is promoted by decidualized endometrial stromal cells and enhanced by heparin-binding epidermal growth factor-like growth factor and interleukin-1 β.

abstract：:The aim of this study was to investigate patterns of gene expression in placental samples from patients with preeclampsia (PE), persistent bilateral uterine artery notching (without PE), and normal controls. This study included placental tissue from nine women with PE, seven with uncomplicated pregnancies and five wit...

journal_title：Molecular human reproduction

authors： Hansson SR,Chen Y,Brodszki J,Chen M,Hernandez-Andrade E,Inman JM,Kozhich OA,Larsson I,Marsál K,Medstrand P,Xiang CC,Brownstein MJ

更新日期：2006-03-01 00:00:00

abstract：:SRY directs testicular development. It has been suggested that the only high-mobility group (HMG) box of the SRY is important for the function of this protein; however, other studies have suggested that the N- and C-terminal regions are also involved in this process. Herein, we analysed and compared in vitro the DNA-b...

journal_title：Molecular human reproduction

authors： Sánchez-Moreno I,Coral-Vázquez R,Méndez JP,Canto P

更新日期：2008-06-01 00:00:00

abstract：:Folate deficiency has been linked to a wide range of pregnancy disorders. Most research about folate-deficiency has focused on the embryo itself, little attention has been paid to possible effects on the placenta. According to our results, the morphology of the placenta, endocrine function, and the expression of genes...

journal_title：Molecular human reproduction

authors： Yin X,Gao R,Geng Y,Chen X,Liu X,Mu X,Ding Y,Wang Y,He J

更新日期：2019-06-06 00:00:00

abstract：:G12 monoclonal antibody (mAb), one of a library of constructed mAb directed against human sperm proteins, was found by immunoperoxidase staining to label the post-acrosomal and neck regions of fixed human cauda epididymal and ejaculated spermatozoa. Epithelium and fluid of caput epididymis were strongly labelled while...

journal_title：Molecular human reproduction

authors： Lefèvre A,Martin Ruiz C,Chokomian S,Duquenne C,Finaz C

更新日期：1997-06-01 00:00:00

abstract：:Retrograde flow of menstrual blood cells during menstruation is considered as the dominant theory for the development of endometriosis. Moreover, current evidence suggests that endometrial-derived stem cells are key players in the pathogenesis of endometriosis. In particular, endometrial stromal stem cells have been s...

journal_title：Molecular human reproduction

authors： Nikoo S,Ebtekar M,Jeddi-Tehrani M,Shervin A,Bozorgmehr M,Vafaei S,Kazemnejad S,Zarnani AH

更新日期：2014-09-01 00:00:00

abstract：:Ever since work on pluripotency induction was originally published, reporting the reprogramming of somatic cells to induced pluripotent stem cells (iPS cells) by the ectopic expression of the four transcription factors Oct4, Sox2, Klf4 and c-Myc, high expectations regarding their potential use for regenerative medicin...

journal_title：Molecular human reproduction

authors： Masip M,Veiga A,Izpisúa Belmonte JC,Simón C

更新日期：2010-11-01 00:00:00

abstract：:The process of sperm chromatin decondensation occurs when a spermatozoon enters an ovum. Protamine disulphide bonds are reduced to SH and the polycationic protamines combine with the polyanionic egg protein, nucleoplasmin, thus being stripped from DNA which then combines with histones. Defective chromatin decondensati...

journal_title：Molecular human reproduction

authors： Samocha-Bone D,Lewin LM,Weissenberg R,Madgar Y,Soffer Y,Shochat L,Golan R

更新日期：1998-02-01 00:00:00

abstract：:Epidemiological and experimental animal studies show that suboptimal environments in fetal and neonatal life exert a profound influence on physiological function and risk of diseases in adult life. The concepts of the 'developmental programming' and Developmental Origins of Health and Diseases (DOHaD) have become well...

journal_title：Molecular human reproduction

authors： Chen T,Liu HX,Yan HY,Wu DM,Ping J

更新日期：2016-08-01 00:00:00

abstract：Study Question:Can endometrial mesenchymal stromal cells (E-MSCs) differentiate into endothelial cells in an in vitro co-culture system with human umbilical vein endothelial cells (HUVECs)? Summary Answer:E-MSCs can acquire endothelial markers and function in a direct co-culture system with HUVECs. What is Known Alre...

journal_title：Molecular human reproduction

authors： Canosa S,Moggio A,Brossa A,Pittatore G,Marchino GL,Leoncini S,Benedetto C,Revelli A,Bussolati B

更新日期：2017-03-01 00:00:00

abstract：:Villous tissues from 30 spontaneous abortions and the same number of artificial abortions were obtained and analysed for the frequency of polyploid cells. Single cell suspensions were made from these tissues without culture and the ploidy of > 100 cells was analysed. Trisomies of chromosomes 17 and 4 have rarely been ...

journal_title：Molecular human reproduction

authors： Horiuchi I,Hashimoto T,Tsuji Y,Shimada H,Furuyama J,Koyama K

更新日期：1997-05-01 00:00:00

abstract：:Indoleamine 2,3-dioxygenase-1 (IDO1) is an intracellular enzyme that catalyses essential amino acid tryptophan along the kynurenine pathway. The aim of this study was to determine the impact of IDO1 expression on the biological characteristic of the endometrial stromal cells (ESCs). IDO1, cyclooxygenase-2 (COX-2) and ...

journal_title：Molecular human reproduction

authors： Mei J,Jin LP,Ding D,Li MQ,Li DJ,Zhu XY

更新日期：2012-10-01 00:00:00

abstract：:Tumour necrosis factor-alpha (TNF-alpha is a pleiotropic cytokine synthesized throughout the female reproductive tract. Even though evidence has accumulated that supports its role in autocrine and paracrine processes, its expression and function in the human endometrium are still not completely understood. To gain a b...

journal_title：Molecular human reproduction

authors： von Wolff M,Classen-Linke I,Heid D,Krusche CA,Beier-Hellwig K,Karl C,Beier HM

更新日期：1999-02-01 00:00:00

abstract：STUDY QUESTION:Could a more detailed evaluation of marmoset spermatogonial morphology, kinetics and niches using high-resolution light microscopy (HRLM) lead to new findings? SUMMARY ANSWER:Three subtypes of marmoset undifferentiated spermatogonia, which were not evenly distributed in terms of number and position alon...

journal_title：Molecular human reproduction

authors： Caldeira-Brant AL,Eras-Garcia L,Alves-Freitas D,Almeida FRCL,Chiarini-Garcia H

更新日期：2018-06-01 00:00:00

abstract：:Binder of SPerm (BSP) proteins are a family of proteins expressed exclusively in the male reproductive tract (seminal vesicles or epididymis) of several mammalian species. They are known to promote capacitation, a sperm maturation step essential for fertilization. Our recent studies have shown that in human, the Binde...

journal_title：Molecular human reproduction

authors： Plante G,Thérien I,Lachance C,Leclerc P,Fan J,Manjunath P

更新日期：2014-05-01 00:00:00

abstract：:Prostaglandins (PG) E2 and F2 alpha are produced by the human corpus luteum in significant amounts and they are known to exert local luteotrophic and luteolytic effects respectively. The present study determined how PGE2 and PGF2 alpha are involved in the regulation of inhibin A, a granulosa cell-derived glycoprotein ...

journal_title：Molecular human reproduction

authors： Erämaa M,Ritvos O

更新日期：1996-11-01 00:00:00

abstract：:Glycoforms of recombinant human follicle stimulating hormone (rhFSH) (Org 32489, Puregon) were characterized using concanavalin A lectin affinity chromatography to reveal information about the internal carbohydrate complexity (extent of carbohydrate side-chain branching) of the preparations. The rhFSH glycoforms were ...

journal_title：Molecular human reproduction

authors： Harris SD,Anobile CJ,McLoughlin JD,Mitchell R,Lambert A,Robertson WR

更新日期：1996-10-01 00:00:00

abstract：:Mitotic centromere-associated kinesin (MCAK) is an ATP-dependent microtubule (MT) depolymerase regulated by Aurora kinase (AURK) phosphorylation and implicated in resolution of improper MT attachments in mitosis. Distribution of MCAK was studied in oocyte maturation by anti-MCAK antibody, anti-tubulin antibody, anti-A...

journal_title：Molecular human reproduction

authors： Vogt E,Sanhaji M,Klein W,Seidel T,Wordeman L,Eichenlaub-Ritter U

更新日期：2010-09-01 00:00:00

abstract：:No phenotypic effect is observed in most inversion heterozygotes. However, reproductive risks may occur in the form of infertility, spontaneous abortions or chromosomally unbalanced children as a consequence of meiotic recombination between inverted and non-inverted chromosomes. An odd number of crossovers within the ...

journal_title：Molecular human reproduction

authors： Chantot-Bastaraud S,Ravel C,Berthaut I,McElreavey K,Bouchard P,Mandelbaum J,Siffroi JP

更新日期：2007-01-01 00:00:00

abstract：:The clinical application of quantitative fluorescent polymerase chain reaction (QF-PCR) for rapid prenatal detection of chromosome aneuploidies has been limited in most studies to the detection of autosomal trisomies. Recently it has been shown that a newly identified highly polymorphic marker, termed X22, which maps ...

journal_title：Molecular human reproduction

authors： Cirigliano V,Ejarque M,Cañadas MP,Lloveras E,Plaja A,Perez MM,Fuster C,Egozcue J

更新日期：2001-10-01 00:00:00

abstract：STUDY QUESTION:Can a pre-in vitro maturation (pre-IVM) medium containing signaling molecules rather than chemical/pharmaceutical agents, sustain meiotic arrest and improve developmental competence of in vitro matured oocytes in CF1 outbred mice? SUMMARY ANSWER:A short 2 h period of pre-IVM prevents spontaneous meiotic...

journal_title：Molecular human reproduction

authors： Santiquet NW,Greene AF,Becker J,Barfield JP,Schoolcraft WB,Krisher RL

更新日期：2017-09-01 00:00:00

abstract：:Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is the most common defect in fatty acid oxidation. The disease is inherited in an autosomal recessive fashion (carrier frequency around 1 in 70) and probably affects as many as 1 in 10000 new-borns. Affected children usually present within the two first years of li...

journal_title：Molecular human reproduction

authors： Sermon K,Henderix P,Lissens W,De Vos A,Vandervorst M,Vanderfaeillie A,Vamos E,Van Steirteghem A,Liebaers I

更新日期：2000-12-01 00:00:00

abstract：:Mouse cauda epididymis were in-vivo transfected using the lipid FuGENE 6 as gene vector. Two gene constructions were employed: the p-GeneGRIP which codifies for the Green Fluorescent Protein (GFP) and the pSEAP-control that expresses an alkaline phosphatase as a secretion. Transfection was detected by fluorescence and...

journal_title：Molecular human reproduction

authors： Esponda P,Carballada R

更新日期：2009-06-01 00:00:00

abstract：:Follicular prostaglandin concentrations increase following the gonadotrophin surge in domestic animals and rodents approximately 10 h before follicle rupture, suggesting a unifying role for prostaglandins in the timing of ovulation. However, little is known about prostaglandin production by the primate ovulatory folli...

journal_title：Molecular human reproduction

authors： Duffy DM,Stouffer RL

更新日期：2001-08-01 00:00:00

abstract：:Preeclampsia (PE) is a severe pregnancy-induced disorder characterized by hypertension and proteinuria and a leading cause of perinatal maternal-fetal mortality and morbidity in developing countries. Dysregulated human leukocyte antigen (HLA)-G was found in placentas as well as in maternal sera from PE patients; howev...

journal_title：Molecular human reproduction

authors： Tang Y,Liu H,Li H,Peng T,Gu W,Li X

更新日期：2015-09-01 00:00:00

abstract：:Endometriosis, the presence of ectopic endometrial tissue outside the uterine cavity, is a common disease affecting women during their reproductive years. The aim of this study was to identify the molecular mechanism of transcriptional regulation of inflammatory cyclooxygenase-2 (COX-2) gene during endometriosis by hy...

journal_title：Molecular human reproduction

authors： Kim KH,Kim HY,Kim HH,Lee KS,Cheong J

更新日期：2011-11-01 00:00:00

abstract：:Controlling the sex of offspring by the separation of X and Y chromosome-bearing spermatozoa using flow cytometry has been reported as a clinical technique aiding prevention of X-linked diseases. Although this technique has resulted in several hundred normal births in animals and at least one human birth, there is sti...

journal_title：Molecular human reproduction

authors： Catt SL,Sakkas D,Bizzaro D,Bianchi PG,Maxwell WM,Evans G

更新日期：1997-09-01 00:00:00

abstract：STUDY QUESTION:Does A20 regulate mediators involved in the terminal processes of human labour in primary myometrial and amnion cells? SUMMARY ANSWER:A20 is a nuclear factor-kappa B (NF-κB) responsive gene that acts as a negative regulator of NF-κB-induced expression of pro-labour mediators. WHAT IS KNOWN ALREADY:Infl...

journal_title：Molecular human reproduction

authors： Lappas M

更新日期：2017-09-01 00:00:00

abstract：:Cystic fibrosis (CF) is usually considered a rare disease in the Indian population. Two studies have reported on the frequency of cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations in Indian males with congenital absence of the vas deferens (CAVD), however, data on the spectrum of CFTR gene muta...

journal_title：Molecular human reproduction

authors： Sharma H,Mavuduru RS,Singh SK,Prasad R

更新日期：2014-09-01 00:00:00

abstract：:The maternal aorta undergoes substantial functional and structural adaptation in pregnancy. Both aortic diameter and compliance are increased and studies of animal and human gestation indicate that these changes are initiated in early pregnancy and maintained until delivery. The mechanisms underlying aortic adaptation...

journal_title：Molecular human reproduction

authors： Kelly BA,Bond BC,Poston L

更新日期：2004-05-01 00:00:00

abstract：:Gonadotrophins exert a major effect on ovarian development and on the control of fertilization. By stimulating cells with forskolin (FK), it is possible to study which genes are activated by gonadotrophins via the cAMP cascade, and which by alternative pathways. Using RNA isolated from stimulated cells, we found that ...

journal_title：Molecular human reproduction

authors： Sasson R,Rimon E,Dantes A,Cohen T,Shinder V,Land-Bracha A,Amsterdam A

更新日期：2004-05-01 00:00:00