Expansion of human trophoblastic spheroids is promoted by decidualized endometrial stromal cells and enhanced by heparin-binding epidermal growth factor-like growth factor and interleukin-1 β.


:Successful pregnancy in humans depends on deep invasion of the maternal decidua by extravillous trophoblast cells (EVTs), a process regulated by autocrine and paracrine signals in the decidual-trophoblast microenvironment. Here we examined whether trophoblast invasion is affected by decidual differentiation of endometrial stromal cells (ESC) and modulated locally by cytokines and growth factors. Trophoblast spheroids were generated from the EVT-derived cell line AC-1M88 and placed onto monolayers of either undifferentiated or decidualized ESC, or directly onto tissue culture surface. Co-cultures were treated with epidermal growth factor (EGF), hepatocyte growth factor, heparin-binding EGF-like growth factor (HB-EGF), interleukin-1β (IL-1β) and leukaemia inhibitory factor (LIF). Expansion of spheroids over 2-3 days was significantly enhanced by a monolayer of undifferentiated ESC compared with tissue culture surface and further increased if ESC had been decidualized. HB-EGF and IL-1β, alone or in combination with LIF, stimulated spheroid expansion but only on undifferentiated ESC. CEACAM1, an adhesion molecule implicated in trophoblast invasion, was up-regulated in AC-1M88 cells by conditioned medium from decidualized ESC, and by HB-EGF, IL-1β and LIF in combination. Treatment of ESC with HB-EGF or IL-1β increased the level of the tetraspanin CD82, a metastasis suppressor found in decidual cells at the implantation site. We suggest that decidualized ESC support trophoblast invasion by paracrine signals that may include HB-EGF, IL-1β and LIF.


Mol Hum Reprod


Gonzalez M,Neufeld J,Reimann K,Wittmann S,Samalecos A,Wolf A,Bamberger AM,Gellersen B




Has Abstract


2011-07-01 00:00:00














  • Gene expression profiling of human placentas from preeclamptic and normotensive pregnancies.

    abstract::The aim of this study was to investigate patterns of gene expression in placental samples from patients with preeclampsia (PE), persistent bilateral uterine artery notching (without PE), and normal controls. This study included placental tissue from nine women with PE, seven with uncomplicated pregnancies and five wit...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Hansson SR,Chen Y,Brodszki J,Chen M,Hernandez-Andrade E,Inman JM,Kozhich OA,Larsson I,Marsál K,Medstrand P,Xiang CC,Brownstein MJ

    更新日期:2006-03-01 00:00:00

  • Full-length SRY protein is essential for DNA binding.

    abstract::SRY directs testicular development. It has been suggested that the only high-mobility group (HMG) box of the SRY is important for the function of this protein; however, other studies have suggested that the N- and C-terminal regions are also involved in this process. Herein, we analysed and compared in vitro the DNA-b...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Sánchez-Moreno I,Coral-Vázquez R,Méndez JP,Canto P

    更新日期:2008-06-01 00:00:00

  • Autophagy regulates abnormal placentation induced by folate deficiency in mice.

    abstract::Folate deficiency has been linked to a wide range of pregnancy disorders. Most research about folate-deficiency has focused on the embryo itself, little attention has been paid to possible effects on the placenta. According to our results, the morphology of the placenta, endocrine function, and the expression of genes...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Yin X,Gao R,Geng Y,Chen X,Liu X,Mu X,Ding Y,Wang Y,He J

    更新日期:2019-06-06 00:00:00

  • Characterization and isolation of SOB2, a human sperm protein with a potential role in oocyte membrane binding.

    abstract::G12 monoclonal antibody (mAb), one of a library of constructed mAb directed against human sperm proteins, was found by immunoperoxidase staining to label the post-acrosomal and neck regions of fixed human cauda epididymal and ejaculated spermatozoa. Epithelium and fluid of caput epididymis were strongly labelled while...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Lefèvre A,Martin Ruiz C,Chokomian S,Duquenne C,Finaz C

    更新日期:1997-06-01 00:00:00

  • Menstrual blood-derived stromal stem cells from women with and without endometriosis reveal different phenotypic and functional characteristics.

    abstract::Retrograde flow of menstrual blood cells during menstruation is considered as the dominant theory for the development of endometriosis. Moreover, current evidence suggests that endometrial-derived stem cells are key players in the pathogenesis of endometriosis. In particular, endometrial stromal stem cells have been s...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Nikoo S,Ebtekar M,Jeddi-Tehrani M,Shervin A,Bozorgmehr M,Vafaei S,Kazemnejad S,Zarnani AH

    更新日期:2014-09-01 00:00:00

  • Reprogramming with defined factors: from induced pluripotency to induced transdifferentiation.

    abstract::Ever since work on pluripotency induction was originally published, reporting the reprogramming of somatic cells to induced pluripotent stem cells (iPS cells) by the ectopic expression of the four transcription factors Oct4, Sox2, Klf4 and c-Myc, high expectations regarding their potential use for regenerative medicin...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章,评审


    authors: Masip M,Veiga A,Izpisúa Belmonte JC,Simón C

    更新日期:2010-11-01 00:00:00

  • In-vitro human spermatozoa nuclear decondensation assessed by flow cytometry.

    abstract::The process of sperm chromatin decondensation occurs when a spermatozoon enters an ovum. Protamine disulphide bonds are reduced to SH and the polycationic protamines combine with the polyanionic egg protein, nucleoplasmin, thus being stripped from DNA which then combines with histones. Defective chromatin decondensati...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Samocha-Bone D,Lewin LM,Weissenberg R,Madgar Y,Soffer Y,Shochat L,Golan R

    更新日期:1998-02-01 00:00:00

  • Developmental origins of inflammatory and immune diseases.

    abstract::Epidemiological and experimental animal studies show that suboptimal environments in fetal and neonatal life exert a profound influence on physiological function and risk of diseases in adult life. The concepts of the 'developmental programming' and Developmental Origins of Health and Diseases (DOHaD) have become well...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章,评审


    authors: Chen T,Liu HX,Yan HY,Wu DM,Ping J

    更新日期:2016-08-01 00:00:00

  • Angiogenic properties of endometrial mesenchymal stromal cells in endothelial co-culture: an in vitro model of endometriosis.

    abstract:Study Question:Can endometrial mesenchymal stromal cells (E-MSCs) differentiate into endothelial cells in an in vitro co-culture system with human umbilical vein endothelial cells (HUVECs)? Summary Answer:E-MSCs can acquire endothelial markers and function in a direct co-culture system with HUVECs. What is Known Alre...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Canosa S,Moggio A,Brossa A,Pittatore G,Marchino GL,Leoncini S,Benedetto C,Revelli A,Bussolati B

    更新日期:2017-03-01 00:00:00

  • Direct assessment of triploid cells in mosaic human fetuses by fluorescence in-situ hybridization.

    abstract::Villous tissues from 30 spontaneous abortions and the same number of artificial abortions were obtained and analysed for the frequency of polyploid cells. Single cell suspensions were made from these tissues without culture and the ploidy of > 100 cells was analysed. Trisomies of chromosomes 17 and 4 have rarely been ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Horiuchi I,Hashimoto T,Tsuji Y,Shimada H,Furuyama J,Koyama K

    更新日期:1997-05-01 00:00:00

  • Inhibition of IDO1 suppresses cyclooxygenase-2 and matrix metalloproteinase-9 expression and decreases proliferation, adhesion and invasion of endometrial stromal cells.

    abstract::Indoleamine 2,3-dioxygenase-1 (IDO1) is an intracellular enzyme that catalyses essential amino acid tryptophan along the kynurenine pathway. The aim of this study was to determine the impact of IDO1 expression on the biological characteristic of the endometrial stromal cells (ESCs). IDO1, cyclooxygenase-2 (COX-2) and ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Mei J,Jin LP,Ding D,Li MQ,Li DJ,Zhu XY

    更新日期:2012-10-01 00:00:00

  • Tumour necrosis factor-alpha (TNF-alpha) in human endometrium and uterine secretion: an evaluation by immunohistochemistry, ELISA and semiquantitative RT-PCR.

    abstract::Tumour necrosis factor-alpha (TNF-alpha is a pleiotropic cytokine synthesized throughout the female reproductive tract. Even though evidence has accumulated that supports its role in autocrine and paracrine processes, its expression and function in the human endometrium are still not completely understood. To gain a b...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: von Wolff M,Classen-Linke I,Heid D,Krusche CA,Beier-Hellwig K,Karl C,Beier HM

    更新日期:1999-02-01 00:00:00

  • Spermatogonial behavior in marmoset: a new generation, their kinetics and niche.

    abstract:STUDY QUESTION:Could a more detailed evaluation of marmoset spermatogonial morphology, kinetics and niches using high-resolution light microscopy (HRLM) lead to new findings? SUMMARY ANSWER:Three subtypes of marmoset undifferentiated spermatogonia, which were not evenly distributed in terms of number and position alon...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Caldeira-Brant AL,Eras-Garcia L,Alves-Freitas D,Almeida FRCL,Chiarini-Garcia H

    更新日期:2018-06-01 00:00:00

  • Implication of the human Binder of SPerm Homolog 1 (BSPH1) protein in capacitation.

    abstract::Binder of SPerm (BSP) proteins are a family of proteins expressed exclusively in the male reproductive tract (seminal vesicles or epididymis) of several mammalian species. They are known to promote capacitation, a sperm maturation step essential for fertilization. Our recent studies have shown that in human, the Binde...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Plante G,Thérien I,Lachance C,Leclerc P,Fan J,Manjunath P

    更新日期:2014-05-01 00:00:00

  • Prostaglandin E2 induces inhibin alpha- and beta A-subunit mRNA and secretion of dimeric inhibin A in cultured human granulosa-luteal cells.

    abstract::Prostaglandins (PG) E2 and F2 alpha are produced by the human corpus luteum in significant amounts and they are known to exert local luteotrophic and luteolytic effects respectively. The present study determined how PGE2 and PGF2 alpha are involved in the regulation of inhibin A, a granulosa cell-derived glycoprotein ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Erämaa M,Ritvos O

    更新日期:1996-11-01 00:00:00

  • Internal carbohydrate complexity of the oligosaccharide chains of recombinant human follicle stimulating hormone (Puregon, Org 32489): a comparison with Metrodin and Metrodin-HP.

    abstract::Glycoforms of recombinant human follicle stimulating hormone (rhFSH) (Org 32489, Puregon) were characterized using concanavalin A lectin affinity chromatography to reveal information about the internal carbohydrate complexity (extent of carbohydrate side-chain branching) of the preparations. The rhFSH glycoforms were ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Harris SD,Anobile CJ,McLoughlin JD,Mitchell R,Lambert A,Robertson WR

    更新日期:1996-10-01 00:00:00

  • MCAK is present at centromeres, midspindle and chiasmata and involved in silencing of the spindle assembly checkpoint in mammalian oocytes.

    abstract::Mitotic centromere-associated kinesin (MCAK) is an ATP-dependent microtubule (MT) depolymerase regulated by Aurora kinase (AURK) phosphorylation and implicated in resolution of improper MT attachments in mitosis. Distribution of MCAK was studied in oocyte maturation by anti-MCAK antibody, anti-tubulin antibody, anti-A...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Vogt E,Sanhaji M,Klein W,Seidel T,Wordeman L,Eichenlaub-Ritter U

    更新日期:2010-09-01 00:00:00

  • Sperm-FISH analysis in a pericentric chromosome 1 inversion, 46,XY,inv(1)(p22q42), associated with infertility.

    abstract::No phenotypic effect is observed in most inversion heterozygotes. However, reproductive risks may occur in the form of infertility, spontaneous abortions or chromosomally unbalanced children as a consequence of meiotic recombination between inverted and non-inverted chromosomes. An odd number of crossovers within the ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Chantot-Bastaraud S,Ravel C,Berthaut I,McElreavey K,Bouchard P,Mandelbaum J,Siffroi JP

    更新日期:2007-01-01 00:00:00

  • Clinical application of multiplex quantitative fluorescent polymerase chain reaction (QF-PCR) for the rapid prenatal detection of common chromosome aneuploidies.

    abstract::The clinical application of quantitative fluorescent polymerase chain reaction (QF-PCR) for rapid prenatal detection of chromosome aneuploidies has been limited in most studies to the detection of autosomal trisomies. Recently it has been shown that a newly identified highly polymorphic marker, termed X22, which maps ...

    journal_title:Molecular human reproduction

    pub_type: 临床试验,杂志文章


    authors: Cirigliano V,Ejarque M,Cañadas MP,Lloveras E,Plaja A,Perez MM,Fuster C,Egozcue J

    更新日期:2001-10-01 00:00:00

  • A pre-in vitro maturation medium containing cumulus oocyte complex ligand-receptor signaling molecules maintains meiotic arrest, supports the cumulus oocyte complex and improves oocyte developmental competence.

    abstract:STUDY QUESTION:Can a pre-in vitro maturation (pre-IVM) medium containing signaling molecules rather than chemical/pharmaceutical agents, sustain meiotic arrest and improve developmental competence of in vitro matured oocytes in CF1 outbred mice? SUMMARY ANSWER:A short 2 h period of pre-IVM prevents spontaneous meiotic...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Santiquet NW,Greene AF,Becker J,Barfield JP,Schoolcraft WB,Krisher RL

    更新日期:2017-09-01 00:00:00

  • Preimplantation genetic diagnosis for medium-chain acyl-CoA dehydrogenase (MCAD) deficiency.

    abstract::Medium-chain acyl-CoA dehydrogenase (MCAD) deficiency is the most common defect in fatty acid oxidation. The disease is inherited in an autosomal recessive fashion (carrier frequency around 1 in 70) and probably affects as many as 1 in 10000 new-borns. Affected children usually present within the two first years of li...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Sermon K,Henderix P,Lissens W,De Vos A,Vandervorst M,Vanderfaeillie A,Vamos E,Van Steirteghem A,Liebaers I

    更新日期:2000-12-01 00:00:00

  • In-vivo gene transfer induces transgene expression in cells and secretions of the mouse cauda epididymis.

    abstract::Mouse cauda epididymis were in-vivo transfected using the lipid FuGENE 6 as gene vector. Two gene constructions were employed: the p-GeneGRIP which codifies for the Green Fluorescent Protein (GFP) and the pSEAP-control that expresses an alkaline phosphatase as a secretion. Transfection was detected by fluorescence and...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Esponda P,Carballada R

    更新日期:2009-06-01 00:00:00

  • The ovulatory gonadotrophin surge stimulates cyclooxygenase expression and prostaglandin production by the monkey follicle.

    abstract::Follicular prostaglandin concentrations increase following the gonadotrophin surge in domestic animals and rodents approximately 10 h before follicle rupture, suggesting a unifying role for prostaglandins in the timing of ovulation. However, little is known about prostaglandin production by the primate ovulatory folli...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Duffy DM,Stouffer RL

    更新日期:2001-08-01 00:00:00

  • Hypermethylation of the HLA-G promoter is associated with preeclampsia.

    abstract::Preeclampsia (PE) is a severe pregnancy-induced disorder characterized by hypertension and proteinuria and a leading cause of perinatal maternal-fetal mortality and morbidity in developing countries. Dysregulated human leukocyte antigen (HLA)-G was found in placentas as well as in maternal sera from PE patients; howev...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Tang Y,Liu H,Li H,Peng T,Gu W,Li X

    更新日期:2015-09-01 00:00:00

  • Hypoxia induces expression of COX-2 through the homeodomain transcription factor CDX1 and orphan nuclear receptor SHP in human endometrial cells.

    abstract::Endometriosis, the presence of ectopic endometrial tissue outside the uterine cavity, is a common disease affecting women during their reproductive years. The aim of this study was to identify the molecular mechanism of transcriptional regulation of inflammatory cyclooxygenase-2 (COX-2) gene during endometriosis by hy...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Kim KH,Kim HY,Kim HH,Lee KS,Cheong J

    更新日期:2011-11-01 00:00:00

  • Hoechst staining and exposure to UV laser during flow cytometric sorting does not affect the frequency of detected endogenous DNA nicks in abnormal and normal human spermatozoa.

    abstract::Controlling the sex of offspring by the separation of X and Y chromosome-bearing spermatozoa using flow cytometry has been reported as a clinical technique aiding prevention of X-linked diseases. Although this technique has resulted in several hundred normal births in animals and at least one human birth, there is sti...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Catt SL,Sakkas D,Bizzaro D,Bianchi PG,Maxwell WM,Evans G

    更新日期:1997-09-01 00:00:00

  • A20, an essential component of the ubiquitin-editing protein complex, is a negative regulator of inflammation in human myometrium and foetal membranes.

    abstract:STUDY QUESTION:Does A20 regulate mediators involved in the terminal processes of human labour in primary myometrial and amnion cells? SUMMARY ANSWER:A20 is a nuclear factor-kappa B (NF-κB) responsive gene that acts as a negative regulator of NF-κB-induced expression of pro-labour mediators. WHAT IS KNOWN ALREADY:Infl...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Lappas M

    更新日期:2017-09-01 00:00:00

  • Heterogeneous spectrum of mutations in CFTR gene from Indian patients with congenital absence of the vas deferens and their association with cystic fibrosis genetic modifiers.

    abstract::Cystic fibrosis (CF) is usually considered a rare disease in the Indian population. Two studies have reported on the frequency of cystic fibrosis transmembrane conductance regulator (CFTR) gene mutations in Indian males with congenital absence of the vas deferens (CAVD), however, data on the spectrum of CFTR gene muta...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Sharma H,Mavuduru RS,Singh SK,Prasad R

    更新日期:2014-09-01 00:00:00

  • Aortic adaptation to pregnancy: elevated expression of matrix metalloproteinases-2 and -3 in rat gestation.

    abstract::The maternal aorta undergoes substantial functional and structural adaptation in pregnancy. Both aortic diameter and compliance are increased and studies of animal and human gestation indicate that these changes are initiated in early pregnancy and maintained until delivery. The mechanisms underlying aortic adaptation...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Kelly BA,Bond BC,Poston L

    更新日期:2004-05-01 00:00:00

  • Gonadotrophin-induced gene regulation in human granulosa cells obtained from IVF patients. Modulation of steroidogenic genes, cytoskeletal genes and genes coding for apoptotic signalling and protein kinases.

    abstract::Gonadotrophins exert a major effect on ovarian development and on the control of fertilization. By stimulating cells with forskolin (FK), it is possible to study which genes are activated by gonadotrophins via the cAMP cascade, and which by alternative pathways. Using RNA isolated from stimulated cells, we found that ...

    journal_title:Molecular human reproduction

    pub_type: 杂志文章


    authors: Sasson R,Rimon E,Dantes A,Cohen T,Shinder V,Land-Bracha A,Amsterdam A

    更新日期:2004-05-01 00:00:00