Inhibition of keratin 17 expression with antisense and RNAi strategies: exploring novel therapy for psoriasis.


:Psoriasis is now considered to be a chronic, immune-mediated and inflammatory skin disease. As the precise cause of psoriasis remains unknown, its treatment is challenging for dermatologists. Keratin 17 (K17), an intermediate filament protein, is highly expressed in psoriatic lesions, while not normally expressed in healthy epidermis. Studies have suggested that K17 plays a role in the pathogenesis of psoriasis. However, no study has been performed to determine the potential application of K17 down-regulation as a treatment option for psoriatic lesions. We hypothesized that anti-K17 interference may suppress the development and progression of psoriasis and potentially serve as a novel strategy for the treatment of psoriasis. Therefore, we down-regulated and silenced K17 gene expression in keratinocytes (KCs) using antisense and RNA interference (RNAi) techniques. We found that K17-specific antisense oligonucleotides (ASODN) or siRNAs inhibited proliferation and induced apoptosis in KCs as well as down-regulated K17 expression at both mRNA and protein levels. For our in vivo study, we constructed the SCID-hu xenogeneic transplantation psoriasis mouse model by grafting psoriatic lesions onto SCID mice and topically applied K17-specific ASODN and liposome-encapsulated siRNA to the grafts. We observed morphological and histological improvement in the treated psoriatic grafts. As a result, K17 mRNA and protein expression was significantly decreased in the grafts of the mouse model. Taken together, we conclude that anti-K17 therapy is an effective treatment option for psoriasis, and the K17 molecule, as a new target, may hold tremendous potential for the treatment of psoriasis in the future.


Exp Dermatol


Experimental dermatology


Chang T,Sun L,Wang Y,Wang D,Li W,Li C,Gao T,Liu Y,Wang G




Has Abstract


2011-07-01 00:00:00












  • The efficacy of electrical stimulation in lower extremity cutaneous wound healing: A systematic review.

    abstract::Current gold standard lower extremity cutaneous wound management is not always effective. Cutaneous wounds generate a "current of injury" which is directly involved in wound healing processes. Application of exogenous electrical stimulation has been hypothesised to imitate the natural electric current that occurs in c...

    journal_title:Experimental dermatology

    pub_type: 杂志文章,评审


    authors: Ashrafi M,Alonso-Rasgado T,Baguneid M,Bayat A

    更新日期:2017-02-01 00:00:00

  • The dermal sheath: An emerging component of the hair follicle stem cell niche.

    abstract::Hair follicles cyclically regenerate throughout adult mammalian life, owing to a resident population of epithelial hair follicle stem cells. Stem cell (SC) activity drives bouts of follicle growth, which are periodically interrupted by follicle regression and rest. These phases and the transitions between them are tig...

    journal_title:Experimental dermatology

    pub_type: 杂志文章,评审


    authors: Martino PA,Heitman N,Rendl M

    更新日期:2020-10-02 00:00:00

  • Neuroendocrine regulation of sebocytes -- a pathogenetic link between stress and acne.

    abstract::A causative link between emotional stress and acne has long been postulated. There is mounting evidence that the molecular mechanism underlying this observation is related to the expression of receptors for several neuroendocrine mediators by the sebaceous gland. Recent and ongoing studies have indicated that human se...

    journal_title:Experimental dermatology

    pub_type: 杂志文章,评审


    authors: Zouboulis CC,Böhm M

    更新日期:2004-01-01 00:00:00

  • Non-steroidal and steroidal anti-inflammatory drugs vary in their modulation of dendritic cell function in the elicitation phase of allergic contact dermatitis.

    abstract::The role of dendritic cells (DCs) in allergic contact dermatitis has been clearly demonstrated for the induction phase. However, the situation during the elicitation phase is very complex within a distinct inflammatory response. This study was performed to exploit DC migration in the elicitation phase in a mouse model...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Bäumer W,Krekeler S,DeVries VC,Niedorf F,Tschernig T,Kietzmann M

    更新日期:2006-04-01 00:00:00

  • Placental extracts regulate melanin synthesis in normal human melanocytes with alterations of mitochondrial respiration.

    abstract::Placental extracts have been widely used as skin lightening agents in the Japanese cosmetic market. Here, we show that placental extracts contain factors that can decrease or increase melanin synthesis by normal human melanocytes in vitro in possible association with mitochondrial respiration. When normal human melano...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Yoshimoto S,Ohagi Y,Yoshida M,Yanagi H,Hibino S,Ichihashi M,Ando H

    更新日期:2019-02-01 00:00:00

  • Tropisetron via α7 nicotinic acetylcholine receptor suppresses tumor necrosis factor-α-mediated cell responses of human keratinocytes.

    abstract::Tropisetron is a serotonin receptor (5-HT-R)-modulating agent and approved as an antiemetic for patients undergoing chemotherapy. In the gut, it acts via specific serotonin receptors, 5-HT3 -R, to elicit its beneficial effects against nausea. We investigated whether tropisetron can affect inflammatory cell responses o...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Stegemann A,Böhm M

    更新日期:2019-03-01 00:00:00

  • Nilotinib induction of melanogenesis via reactive oxygen species-dependent JNK activation in B16F0 mouse melanoma cells.

    abstract::Nilotinib (AMN), a second-generation tyrosine kinase inhibitor, induces apoptosis in various cancer cells, and our recent study showed that AMN effectively reduced the viability of human ovarian cancer cells via mitochondrion-dependent apoptosis. The effect of AMN in the melanogenesis of melanoma cells is still unclea...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Chang SP,Huang HM,Shen SC,Lee WR,Chen YC

    更新日期:2018-12-01 00:00:00

  • Alternative approaches to antifungal therapies.

    abstract::The expansive use of immunosuppressive medications in fields such as transplantational medicine and oncology, the higher frequency of invasive procedures in an ageing population and the HIV/AIDS pandemic have increased the frequency of systemic fungal infections. At the same time, increased resistance of pathogenic fu...

    journal_title:Experimental dermatology

    pub_type: 杂志文章,评审


    authors: Mehra T,Köberle M,Braunsdorf C,Mailänder-Sanchez D,Borelli C,Schaller M

    更新日期:2012-10-01 00:00:00

  • Serum level of IL-4 predicts response to topical immunotherapy with diphenylcyclopropenone in alopecia areata.

    abstract:BACKGROUND:This study investigated predictors of response to topical diphenylyclopropenone (DPCP) immunotherapy in patients with alopecia areata (AA). OBJECTIVE:To identify predictors of response, or resistance, to treatment for AA through clinical observations and serum tests. METHODS:Eighty four AA patients were tr...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Gong Y,Zhao Y,Zhang X,Qi S,Li S,Ye Y,Yang J,Caulloo S,McElwee KJ,Zhang X

    更新日期:2020-03-01 00:00:00

  • Expression of the Abca-subfamily of genes in Abcc6-/- mice--upregulation of Abca4.

    abstract::Pseudoxanthoma elasticum (PXE), a heritable multi-system disorder, is caused by mutations in the ABCC6 gene primarily expressed in the liver. Recent analysis of cultured fibroblasts from patients with PXE has suggested compensatory alterations in the expression of the ABCA-subfamily of genes. We have now determined by...

    journal_title:Experimental dermatology

    pub_type: 信件


    authors: Li Q,Uitto J

    更新日期:2011-05-01 00:00:00

  • SIRT2 as a new player in epigenetic programming of keratinocyte differentiation and a candidate tumor suppressor.

    abstract::Epidermal keratinocytes undergo a continuous process of terminal differentiation, which is accompanied by a dramatic change in the expression and composition of keratins. This complex and carefully orchestrated process is regulated by a large number of signal transduction events and transcriptional factors as well as ...

    journal_title:Experimental dermatology

    pub_type: 评论,杂志文章


    authors: Wang M,Yue Z,Paus R,Ramot Y

    更新日期:2014-09-01 00:00:00

  • R164C mutation in FOXQ1 H3 domain affects formation of the hair medulla.

    abstract::A number of single gene mutations in laboratory mice produce hair follicle defects resulting in deformed hair shafts. The radiation-induced (SB/LeJ-Foxq1(sa)) satin mutant mice have a satin-like sheen to their hair and dilute colouration. This sheen is due to failure of the hair shafts to develop normal medullas, whil...

    journal_title:Experimental dermatology

    pub_type: 信件


    authors: Wu B,Herbert Pratt C,Potter CS,Silva KA,Kennedy V,Sundberg JP

    更新日期:2013-03-01 00:00:00

  • Administration of acetylcholine and vasoactive intestinal polypeptide to atopic eczema patients.

    abstract::Responses to acetylcholine (ACh) and vasoactive intestinal polypeptide (VIP) were investigated in atopic eczema (AE) patients. To elucidate the involvement of histamine to ACh-provoked vasoreactions and sensations, we applied a selective H1-antagonist (cetirizine) 3 h prior to the ACh-administration. Solutions of acet...

    journal_title:Experimental dermatology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Rukwied R,Heyer G

    更新日期:1999-02-01 00:00:00

  • Serum amyloid A1 secreted from UV-irradiated keratinocytes induces matrix metalloproteinase-1 in fibroblasts through toll-like receptor 4.

    abstract::Ultraviolet (UV) irradiation on skin triggers photoageing-related phenotypes such as formation of wrinkles. UV ray upregulates matrix metalloproteinase-1 (MMP-1), which in turn degrades extracellular matrix proteins, mostly collagens. Serum amyloid A1 (SAA1) is an acute-phase protein of which plasma concentration incr...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Han S,Jin SP,Oh JH,Seo EY,Park CH,Yoon HS,Lee DH,Chung JH

    更新日期:2016-07-01 00:00:00

  • hMLH1 and hMSH2 gene mutations are present in radial growth-phase cutaneous malignant melanoma cell lines and can be induced further by ultraviolet-B irradiation.

    abstract::Microsatellite instability and reduced expression of mismatch repair proteins were reported in melanomas. However, little is known about mutational changes of the mismatch repair genes in radial growth-phase melanoma especially following UVB irradiation. To investigate these changes, an in vitro system consisting of r...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Hussein MR,Wood GS

    更新日期:2003-12-01 00:00:00

  • Photoprotection against UVAR: effective triterpenoids require a lipid raft stabilizing chemical structure.

    abstract::UVA(Ultraviolet A)-induced gene expression is supposed to be a hallmark for inflammation, for immunosuppression and in long-term cancer formation. In previous studies, we have shown for keratinocytes that physiological doses of UVA radiation result in the upregulation of gene expression mediated by ceramide formation ...

    journal_title:Experimental dermatology

    pub_type: 信件


    authors: Bayer M,Proksch P,Felsner I,Brenden H,Kohne Z,Walli R,Duong TN,Götz C,Krutmann J,Grether-Beck S

    更新日期:2011-11-01 00:00:00

  • Radiofrequency irradiation attenuates angiogenesis and inflammation in UVB-induced rosacea in mouse skin.

    abstract::Rosacea is a skin inflammatory condition accompanied by cutaneous signs such as oedema, flushing, erythema, telangiectasia and pustules. Generally, rosacea is triggered by ultraviolet B (UVB) exposure. When exposed to UVB, skin epidermis thickens and produces elevated levels of pro-inflammatory cytokines, especially k...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Son M,Park J,Oh S,Choi J,Shim M,Kang D,Byun K

    更新日期:2020-05-20 00:00:00

  • The 'psoriatic march': a concept of how severe psoriasis may drive cardiovascular comorbidity.

    abstract::There is increasing awareness that psoriasis is more than 'skin deep'. Several recent reviews focussed on biomarkers indicating the systemic dimension of psoriasis and the aspect of comorbidity psoriasis shares with other chronic inflammatory diseases, such as Crohn's disease and rheumatoid arthritis. Of emerging sign...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Boehncke WH,Boehncke S,Tobin AM,Kirby B

    更新日期:2011-04-01 00:00:00

  • Epidermal growth factor receptor gene numerical aberrations are frequent events in actinic keratoses and invasive cutaneous squamous cell carcinomas.

    abstract::Epidermal growth factor receptor (EGFR) gene amplification and protein overexpression are common in several cancers. EGFR status has seldom been studied in cutaneous squamous carcinomas (SCCs), or their precursors, actinic keratoses (AKs). We evaluated the presence of EGFR genomic aberrations and EGFR protein overexpr...

    journal_title:Experimental dermatology

    pub_type: 信件


    authors: Toll A,Salgado R,Yébenes M,Martín-Ezquerra G,Gilaberte M,Baró T,Solé F,Alameda F,Espinet B,Pujol RM

    更新日期:2010-02-01 00:00:00

  • CD44 variant isoform v10 is expressed on tumor-infiltrating lymphocytes and mediates hyaluronan-independent heterotypic cell-cell adhesion to melanoma cells.

    abstract::CD44 is a family of cell-surface receptors on human lymphocytes that act as co-stimulatory molecules leading to the induction of effector functions in T cells. We have analyzed primary cutaneous malignant melanomas with clinical and histologic signs of tumor regression using immunohistochemistry and observed the predo...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Weimann TK,Wagner C,Goos M,Wagner SN

    更新日期:2003-04-01 00:00:00

  • On the relationship between VDR, RORα and RORγ receptors expression and HIF1-α levels in human melanomas.

    abstract::We analysed the correlation between the expression of HIF-1α (hypoxia-inducible factor 1 alpha), the nuclear receptors: VDR (vitamin D receptor), RORα (retinoic acid receptor-related orphan receptor alpha), and RORγ and CYP24A1 (cytochrome P450 family 24 subfamily A member 1) and CYP27B1 (cytochrome P450 family 27 sub...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Brożyna AA,Jóźwicki W,Jetten AM,Slominski AT

    更新日期:2019-09-01 00:00:00

  • New mechanism for amino acid influx into human epidermal Langerhans cells: L-dopa/proton counter-transport system.

    abstract::We have characterized a stereospecific transport mechanism for L-dopa into human epidermal Langerhans cells (LCs). It is different from any other amino acid transport system. It is highly concentrative, largely pH-independent, and independent of exogenous Na+, glucose and oxygen, and fuelled by a renewable intracellul...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Falck B,Bendsoe N,Ronquist G

    更新日期:2003-10-01 00:00:00

  • Aggressive primary cutaneous B-cell lymphomas show increased Angiopoietin-2-induced angiogenesis.

    abstract::Primary cutaneous large B-cell lymphomas, leg type (PCLBCL/LT) are primary cutaneous B-cell lymphoma (PCBCL) with an intermediate prognosis. Therefore, antracycline-based polychemotherapy combined with rituximab has been recommended as first-line treatment. Yet, despite this regimen, the 5-year survival rate remains 5...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Teichert M,Stumpf C,Booken N,Wobser M,Nashan D,Hallermann C,Mogler C,Müller CS,Becker JC,Moritz RK,Andrulis M,Nicolay JP,Goerdt S,Thomas M,Klemke CD,Augustin HG,Felcht M

    更新日期:2015-06-01 00:00:00

  • Prognostic tools for hypertrophic scar formation based on fundamental differences in systemic immunity.

    abstract::Unpredictable hypertrophic scarring (HS) occurs after approximately 35% of all surgical procedures and causes significant physical and psychological complaints. Parallel to the need to understanding the mechanisms underlying HS formation, a prognostic tool is needed. The objective was to determine whether (systemic) i...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: de Bakker E,van der Putten MAM,Heymans MW,Spiekstra SW,Waaijman T,Butzelaar L,Negenborn VL,Beekman VK,Akpinar EO,Rustemeyer T,Niessen FB,Gibbs S

    更新日期:2021-01-01 00:00:00

  • Egr-1 is a key regulator of IL-17A-induced psoriasin upregulation in psoriasis.

    abstract::The early growth response (Egr)-1 is a transcriptional factor which plays an important role in the regulation of cell growth, differentiation, cell survival and immune responses. Emerging evidences including our data demonstrate that the Egr-1 expression is up-regulated in the psoriatic skin lesions. The purpose of th...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Jeong SH,Kim HJ,Jang Y,Ryu WI,Lee H,Kim JH,Bae HC,Choi JE,Kye YC,Son SW

    更新日期:2014-12-01 00:00:00

  • Inhibition of intercellular adhesio nmolecule-1 (ICAM-1) expression in ultraviolet B-irradiated human antigen-presenting cells is restored after repair of cyclobutane pyrimidine dimers.

    abstract::The present study assessed the molecular mechanism underlying ultraviolet (UV) B radiation-induced inhibition of the expression of the adhesion molecule ICAM-1 in human antigen-presenting cells (APC). UVB radiation-induced inhibition of ICAM-I expression in human peripheral blood monocytes was associated with the gene...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Grewe M,Stege H,Vink A,Klammer M,Ruzicka T,Roza L,Krutmann J

    更新日期:2000-12-01 00:00:00

  • The MEK/ERK signalling cascade is required for sonic hedgehog signalling pathway-mediated enhancement of proliferation and inhibition of apoptosis in normal keratinocytes.

    abstract::Keratinocytes (KCs) play a critical role in maintaining the cutaneous structure and are involved in various physiological and pathologic processes of the skin. Many inflammatory skin diseases and skin cancers result from excessive proliferation and insufficient apoptosis of KCs. Recent data suggested that the sonic he...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Liu H,Jian Q,Xue K,Ma C,Xie F,Wang R,Liao W,Liu Y,Chi S,Li C

    更新日期:2014-12-01 00:00:00

  • Immune privilege of skin stem cells: What do we know and what can we learn?

    abstract::The skin forms a barrier that prevents dehydration and keeps us safe from pathogens. To ensure proper function, the skin possesses a myriad of stem cell populations that are essential for maintenance and repair upon damage. In order to protect, the skin is also an active immunological site, with abundant resident immu...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Agudo J

    更新日期:2020-10-26 00:00:00

  • Decorin-expressing adenovirus decreases collagen synthesis and upregulates MMP expression in keloid fibroblasts and keloid spheroids.

    abstract::Decorin is a natural transforming growth factor-β1 (TGF-β1) antagonist. Reduced decorin synthesis is associated with dermal scarring, and increased decorin expression appears to reduce scar tissue formation. To investigate the therapeutic potential of decorin for keloids, human dermal fibroblasts (HDFs) and keloid-der...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Lee WJ,Ahn HM,Roh H,Na Y,Choi IK,Lee JH,Kim YO,Lew DH,Yun CO

    更新日期:2015-08-01 00:00:00

  • Transcriptomic and lipidomic profiling of eicosanoid/docosanoid signalling in affected and non-affected skin of human atopic dermatitis patients.

    abstract::Lipoxygenases (LOX) and cyclooxygenase (COX) are the main enzymes for PUFA metabolism to highly bio-active prostaglandins, leukotrienes, thromboxanes, lipoxins, resolvins and protectins. LOX and COX pathways are important for the regulation of pro-inflammatory or pro-resolving metabolite synthesis and metabolism for v...

    journal_title:Experimental dermatology

    pub_type: 杂志文章


    authors: Töröcsik D,Weise C,Gericke J,Szegedi A,Lucas R,Mihaly J,Worm M,Rühl R

    更新日期:2019-02-01 00:00:00