Treatment of TBI with collagen scaffolds and human marrow stromal cells increases the expression of tissue plasminogen activator.


:This study examines the effects of combination therapy of collagen scaffolds and human marrow stromal cells (hMSCs) on the expression of tissue plasminogen activator (tPA) after traumatic brain injury (TBI) in rats. Adult male Wistar rats (n=48) were injured with controlled cortical impact and treated either with scaffolds suffused with hMSCs (3×10(6)) or hMSCs (3×10(6)) alone transplanted into the lesion cavity 1 week after TBI. A control group was treated with saline. Neurological function was assessed using the Morris Water Maze test (MWM) and modified Neurological Severity Scores (mNSS). The rats were sacrificed 14 days after TBI and brain samples were processed for immunohistochemical analysis and quantitative Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) studies. Enhanced functional improvement was observed on both the mNSS and MWM tests in the scaffold+hMSC-treated group compared to the other two groups. Immunostaining with anti-human mitochondrial antibody (E5204) showed more hMSCs in the injury zone of the scaffold+hMSC group compared to the hMSC-alone group. Triple staining showed that more neurons were tPA-positive in the scaffold+hMSC group compared to the other two groups (p<0.05). Western blot analysis and qRT-PCR showed that scaffold+hMSC and hMSC-alone treatment enhanced the expression of tPA compared to controls (p<0.05), but tPA expression was significantly greater in the scaffold+hMSC group. The induction of tPA by hMSCs after TBI may be one of the mechanisms involved in promoting functional improvement after TBI.


J Neurotrauma


Journal of neurotrauma


Mahmood A,Qu C,Ning R,Wu H,Goussev A,Xiong Y,Irtenkauf S,Li Y,Chopp M




Has Abstract


2011-07-01 00:00:00












  • Health-related quality of life in traumatic brain injury: is a proxy report necessary?

    abstract::Despite its importance to care, clinicians and researchers often discount patient-reported outcomes in favor of proxy reports, in persons with traumatic brain injury (TBI). The rationale relates to concerns about lack of awareness of patients regarding their functioning. However, although lack of awareness occurs in s...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Machamer J,Temkin N,Dikmen S

    更新日期:2013-11-15 00:00:00

  • Early enteral nutrition and clinical outcomes of severe traumatic brain injury patients in acute stage: a multi-center cohort study.

    abstract::Guidelines for patients with severe traumatic brain injury (sTBI) published in 2007 recommend providing early nutrition after trauma. Early enteral nutrition (EN) started within 48 h post-injury reduces clinical malnutrition, prevents bacterial translocation from the gastrointestinal tract, and improves outcome in sTB...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章,多中心研究


    authors: Chiang YH,Chao DP,Chu SF,Lin HW,Huang SY,Yeh YS,Lui TN,Binns CW,Chiu WT

    更新日期:2012-01-01 00:00:00

  • Mechanical Design and Analysis of a Unilateral Cervical Spinal Cord Contusion Injury Model in Non-Human Primates.

    abstract::Non-human primate (NHP) models of spinal cord injury better reflect human injury and provide a better foundation to evaluate potential treatments and functional outcomes. We combined finite element (FE) and surrogate models with impact data derived from in vivo experiments to define the impact mechanics needed to gene...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Sparrey CJ,Salegio EA,Camisa W,Tam H,Beattie MS,Bresnahan JC

    更新日期:2016-06-15 00:00:00

  • Noninvasive Quantification of Axonal Loss in the Presence of Tissue Swelling in Traumatic Spinal Cord Injury Mice.

    abstract::Neuroimaging plays an important role in assessing axonal pathology after traumatic spinal cord injury. However, coexisting inflammation confounds imaging assessment of the severity of axonal injury. Herein, we applied diffusion basis spectrum imaging (DBSI) to quantitatively differentiate and quantify underlying patho...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Lin TH,Sun P,Hallman M,Hwang FC,Wallendorf M,Ray WZ,Spees WM,Song SK

    更新日期:2019-08-01 00:00:00

  • Motoneuron loss associated with chronic locomotion impairments after spinal cord contusion in the rat.

    abstract::Information on the nature of deficits and adaptive mechanisms occurring after spinal cord injury is essential to the design of strategies for promoting functional recovery. Motor impairments and compensations were quantified by three-dimensional kinematic analysis in freely walking rats, 6 months after mild cervical (...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Collazos-Castro JE,Soto VM,Gutiérrez-Dávila M,Nieto-Sampedro M

    更新日期:2005-05-01 00:00:00

  • Severity of spinal cord injury in adult and infant rats after vertebral dislocation depends upon displacement but not speed.

    abstract::Spinal cord injury (SCI) is less common in children than in adults, but in children it is generally more severe. Spinal loading conditions (speed and displacement) are also thought to affect SCI severity, but the relationship between these parameters is not well understood. This study aimed to investigate the effects ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Lau NS,Gorrie CA,Chia JY,Bilston LE,Clarke EC

    更新日期:2013-08-01 00:00:00

  • Mode changes of cerebrovascular pressure transmission induced by cerebral vasodilation.

    abstract::Changes in the modes of cerebrovascular pressure transmission during cerebral vasodilation induced by hypercapnic challenge were examined as a means for developing the basis for a bedside method to evaluate regulation of cerebral blood flow. Recordings of arterial blood pressure (ABP) and intracranial pressure (ICP) o...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Daley ML,Pourcyrous M,Timmons SD,Leffler CW

    更新日期:2007-03-01 00:00:00

  • Robotic Assessment of Motor, Sensory, and Cognitive Function in Acute Sport-Related Concussion and Recovery.

    abstract::There is a need for better tools to objectively, reliably, and precisely assess neurological function after sport-related concussion (SRC). The aim of this study was to use a robotic device (Kinesiological Instrument for Normal and Altered Reaching Movements; KINARM) to quantify neurological impairments in athletes ac...

    journal_title:Journal of neurotrauma

    pub_type: 临床试验,杂志文章


    authors: Mang CS,Whitten TA,Cosh MS,Scott SH,Wiley JP,Debert CT,Dukelow SP,Benson BW

    更新日期:2019-01-15 00:00:00

  • Neurosurgical Treatment Variation of Traumatic Brain Injury: Evaluation of Acute Subdural Hematoma Management in Belgium and The Netherlands.

    abstract::Several recent global traumatic brain injury (TBI) initiatives rely on practice variation in diagnostic and treatment methods to answer effectiveness questions. One of these scientific dilemmas, the surgical management of the traumatic acute subdural hematoma (ASDH) might be variable among countries, among centers wit...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: van Essen TA,de Ruiter GC,Kho KH,Peul WC

    更新日期:2017-02-15 00:00:00

  • Cortical excitability changes in patients with sleep-wake disturbances after traumatic brain injury.

    abstract::Although chronic sleepiness is common after head trauma, the cause remains unclear. Transcranial magnetic stimulation (TMS) represents a useful complementary approach in the study of sleep pathophysiology. We aimed to determine in this study whether post-traumatic sleep-wake disturbances (SWD) are associated with chan...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Nardone R,Bergmann J,Kunz A,Caleri F,Seidl M,Tezzon F,Gerstenbrand F,Trinka E,Golaszewski S

    更新日期:2011-07-01 00:00:00

  • Effect of traumatic brain injury on mouse spatial and nonspatial learning in the Barnes circular maze.

    abstract::Controlled cortical impact (CCI) is a relatively new model of traumatic brain injury in the mouse, which, in combination with behavioral and histological methods, has potential for elucidating underlying mechanisms of neurodegeneration using genetically altered animals. Previously, we have demonstrated impaired spatia...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Fox GB,Fan L,LeVasseur RA,Faden AI

    更新日期:1998-12-01 00:00:00

  • Traumatic brain injury and long-term quality of life: findings from an Australian study.

    abstract::Recent calls have been made for the inclusion of health-related quality of life (HRQoL) in traumatic brain injury studies. This study reports the impact of TBI on traditional measures (general health, depression, social isolation, labor force participation), self-assessed health function status using the SF-36 version...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Hawthorne G,Gruen RL,Kaye AH

    更新日期:2009-10-01 00:00:00

  • Pharmacologic strategies in the treatment of experimental spinal cord injury.

    abstract::Remarkable advances have been made in pharmacologic treatments of acute and chronic spinal cord injury. The recent National Acute Spinal Cord Injury Study (NASCIS) showed that very high dose methylprednisolone given within 8 hr after injury improves neurologic recovery. The mechanism is believed to be inhibition of li...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章,评审


    authors: Nockels R,Young W

    更新日期:1992-03-01 00:00:00

  • Inhibition of Transient Receptor Potential Vanilloid 1 Attenuates Blood-Brain Barrier Disruption after Traumatic Brain Injury in Mice.

    abstract::Transient receptor potential vanilloid 1 (TRPV1) is expressed widely in the central nervous system and is activated by various stimuli. Inhibiting TRPV1 has neuroprotective effects in cerebral ischemia. The role of inhibiting TRPV1 to maintain blood-brain barrier (BBB) integrity after traumatic brain injury (TBI) rema...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Yang DX,Jing Y,Liu YL,Xu ZM,Yuan F,Wang ML,Geng Z,Tian HL

    更新日期:2019-04-15 00:00:00

  • Alterations in Cholinergic Pathways and Therapeutic Strategies Targeting Cholinergic System after Traumatic Brain Injury.

    abstract::Traumatic brain injury (TBI) results in varying degrees of disability in a significant number of persons annually. The mechanisms of cognitive dysfunction after TBI have been explored in both animal models and human clinical studies for decades. Dopaminergic, serotonergic, and noradrenergic dysfunction has been descri...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章,评审


    authors: Shin SS,Dixon CE

    更新日期:2015-10-01 00:00:00

  • Neuron-specific enolase serum levels after controlled cortical impact injury in the rat.

    abstract::The aim of this study was to investigate the time course and the correlation of neuron-specific enolase (NSE) serum levels to the severity of traumatic brain injury in rats. Sixty-five male Wistar rats were subjected to severe cortical impact injury (100 PSI, 2 mm deformation). Blood samples were drawn directly after ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Woertgen C,Rothoerl RD,Brawanski A

    更新日期:2001-05-01 00:00:00

  • Risk of Depression after Traumatic Brain Injury in a Large National Sample.

    abstract::Depression is associated with poorer recovery after traumatic brain injury (TBI), yet awareness of depression risk post-TBI among providers and patients is low. The aim of this study was to estimate risk of depression post-TBI among adults 18 years of age and older and to identify risk factors associated with developi...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Albrecht JS,Barbour L,Abariga SA,Rao V,Perfetto EM

    更新日期:2019-01-15 00:00:00

  • Acupressure as a non-pharmacological intervention for traumatic brain injury (TBI).

    abstract::Acupressure is a complementary and alternative medicine (CAM) treatment using fingertips to stimulate acupoints on the skin. Although suggested to improve cognitive function, acupressure has not been previously investigated with a controlled design in traumatic brain injury (TBI) survivors, who could particularly bene...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章,随机对照试验


    authors: McFadden KL,Healy KM,Dettmann ML,Kaye JT,Ito TA,Hernández TD

    更新日期:2011-01-01 00:00:00

  • Age Moderates the Effects of Traumatic Brain Injury on Beta-Amyloid Plaque Load in APP/PS1 Mice.

    abstract::Traumatic brain injury (TBI) has been identified as a risk factor for Alzheimer's disease (AD). However, how such neural damage contributes to AD pathology remains unclear; specifically, the relationship between the timing of a TBI relative to aging and the onset of AD pathology is not known. In this study, we have ex...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Collins JM,King AE,Woodhouse A,Kirkcaldie MTK,Vickers JC

    更新日期:2019-06-01 00:00:00

  • Apoptotic versus necrotic characteristics of retinal ganglion cell death after partial optic nerve injury.

    abstract::We have investigated time course and characteristics of retinal ganglion cell (RGC) death after partial optic nerve injury. In situ end labeling of DNA fragments with the terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine (dUTP)-biotin nick end labeling (TUNEL) method revealed the presence of apoptotic ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Bien A,Seidenbecher CI,Böckers TM,Sabel BA,Kreutz MR

    更新日期:1999-02-01 00:00:00

  • Neuroprotective anti-apoptosis effect of estrogens in traumatic brain injury.

    abstract::Traumatic brain injury (TBI) is a leading cause of death and functional disability in western countries, affecting mostly young patients. Despite intense and sustained efforts deployed for the development of new therapeutic strategies, no clinical benefit has been shown by any of the investigated compounds. Increasing...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Soustiel JF,Palzur E,Nevo O,Thaler I,Vlodavsky E

    更新日期:2005-03-01 00:00:00

  • Cerebrospinal fluid inflammatory cytokines and biomarkers of injury severity in acute human spinal cord injury.

    abstract::There is an urgent need for both the scientific development and clinical validation of novel therapies for acute spinal cord injury (SCI). The scientific development of novel therapies would be facilitated by a better understanding of the acute pathophysiology of human SCI. Clinical validation of such therapies would ...

    journal_title:Journal of neurotrauma

    pub_type: 临床试验,杂志文章


    authors: Kwon BK,Stammers AM,Belanger LM,Bernardo A,Chan D,Bishop CM,Slobogean GP,Zhang H,Umedaly H,Giffin M,Street J,Boyd MC,Paquette SJ,Fisher CG,Dvorak MF

    更新日期:2010-04-01 00:00:00

  • Post-acute brain injury urinary signature: a new resource for molecular diagnostics.

    abstract::Heterogeneity within brain injury presents a challenge to the development of informative molecular diagnostics. Recent studies show progress, particularly in cerebrospinal fluid, with biomarker assays targeting one or a few structural proteins. Protein-based assays in peripheral fluids, however, have been more challen...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Ottens AK,Stafflinger JE,Griffin HE,Kunz RD,Cifu DX,Niemeier JP

    更新日期:2014-04-15 00:00:00

  • Survival Analysis-Based Human Head Injury Risk Curves: Focus on Skull Fracture.

    abstract::Head contact-induced loads can result in skull fractures and/or brain injuries. While skull fractures have been produced from post-mortem human cadaver surrogates (PMHS), injury probability curves describing their structural responses have not been developed. The objectives of this study were to develop skull fracture...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Yoganandan N,Banerjee A

    更新日期:2018-06-01 00:00:00

  • Cortical Thickness in Mild Traumatic Brain Injury.

    abstract::Magnetic resonance imaging data were acquired at ∼24 h and ∼3 months post-injury on mild traumatic brain injury (mTBI; n = 75) and orthopedic injury (n = 60) cohorts. The mTBI subjects were randomly assigned to a treatment group with atorvastatin or a non-treatment mTBI group. The treatment group was further divided i...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章,随机对照试验


    authors: Govindarajan KA,Narayana PA,Hasan KM,Wilde EA,Levin HS,Hunter JV,Miller ER,Patel VK,Robertson CS,McCarthy JJ

    更新日期:2016-10-15 00:00:00

  • Efficacy of progesterone following a moderate unilateral cortical contusion injury.

    abstract::Traumatic brain injury (TBI) results in an accumulation of edema and loss of brain tissue. Progesterone (PROG) has been reported to reduce edema and cortical tissue loss in a bilateral prefrontal cortex injury. This study tests the hypothesis that PROG is neuroprotective following a unilateral parietal cortical contus...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Gilmer LK,Roberts KN,Scheff SW

    更新日期:2008-06-01 00:00:00

  • Spinal Cord Injury Caused by Stab Wounds: Incidence, Natural History, and Relevance for Future Research.

    abstract::Spinal cord injury caused by stab wounds (SCISW) results from a partial or complete transection of the cord, and presents opportunities for interventional research. It is recognized that there is low incidence, but little is known about the natural history or the patient's suitability for long-term clinical outcome st...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: McCaughey EJ,Purcell M,Barnett SC,Allan DB

    更新日期:2016-08-01 00:00:00

  • The novel compound LOE 908 attenuates acute neuromotor dysfunction but not cognitive impairment or cortical tissue loss following traumatic brain injury in rats.

    abstract::Experimental traumatic brain injury (TBI) initiates massive disturbances in Ca2+ concentrations in the brain that may contribute to neuronal damage. Intracellular Ca2+ may be elevated via influx through voltage-operated cation channels, ligand-gated ionotropic channels, and store-operated cation channels (SOCs). In th...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Cheney JA,Brown AL,Bareyre FM,Russ AB,Weisser JD,Ensinger HA,Leusch A,Raghupathi R,Saatman KE

    更新日期:2000-01-01 00:00:00

  • Variation in PPP3CC Genotype Is Associated with Long-Term Recovery after Severe Brain Injury.

    abstract::After experimental traumatic brain injury (TBI), calcineurin is upregulated; blocking calcineurin is associated with improved outcomes. In humans, variation in the calcineurin A-gamma gene (PPP3CC) has been associated with neuropsychiatric disorders, though any role in TBI recovery remains unknown. This study examines...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Osier ND,Bales JW,Pugh B,Shin S,Wyrobek J,Puccio AM,Okonkwo DO,Ren D,Alexander S,Conley YP,Dixon CE

    更新日期:2017-01-01 00:00:00

  • Hypothermia reduces cytotoxic edema and metabolic alterations during the acute phase of massive SAH: a diffusion-weighted imaging and spectroscopy study in rats.

    abstract::Acute changes in cerebral perfusion and metabolism after subarachnoid hemorrhage (SAH) have been shown to contribute significantly to acute brain injury. The purpose of this study was to examine the effects of moderate hypothermia on the acute changes after massive experimental SAH as evaluated by diffusion-weighted i...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Schubert GA,Poli S,Schilling L,Heiland S,Thomé C

    更新日期:2008-07-01 00:00:00