Treatment of TBI with collagen scaffolds and human marrow stromal cells increases the expression of tissue plasminogen activator.


:This study examines the effects of combination therapy of collagen scaffolds and human marrow stromal cells (hMSCs) on the expression of tissue plasminogen activator (tPA) after traumatic brain injury (TBI) in rats. Adult male Wistar rats (n=48) were injured with controlled cortical impact and treated either with scaffolds suffused with hMSCs (3×10(6)) or hMSCs (3×10(6)) alone transplanted into the lesion cavity 1 week after TBI. A control group was treated with saline. Neurological function was assessed using the Morris Water Maze test (MWM) and modified Neurological Severity Scores (mNSS). The rats were sacrificed 14 days after TBI and brain samples were processed for immunohistochemical analysis and quantitative Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) studies. Enhanced functional improvement was observed on both the mNSS and MWM tests in the scaffold+hMSC-treated group compared to the other two groups. Immunostaining with anti-human mitochondrial antibody (E5204) showed more hMSCs in the injury zone of the scaffold+hMSC group compared to the hMSC-alone group. Triple staining showed that more neurons were tPA-positive in the scaffold+hMSC group compared to the other two groups (p<0.05). Western blot analysis and qRT-PCR showed that scaffold+hMSC and hMSC-alone treatment enhanced the expression of tPA compared to controls (p<0.05), but tPA expression was significantly greater in the scaffold+hMSC group. The induction of tPA by hMSCs after TBI may be one of the mechanisms involved in promoting functional improvement after TBI.


J Neurotrauma


Journal of neurotrauma


Mahmood A,Qu C,Ning R,Wu H,Goussev A,Xiong Y,Irtenkauf S,Li Y,Chopp M




Has Abstract


2011-07-01 00:00:00












  • Subacute Pain after Traumatic Brain Injury Is Associated with Lower Insular N-Acetylaspartate Concentrations.

    abstract::Persistent pain is experienced by more than 50% of persons who sustain a traumatic brain injury (TBI), and more than 30% experience significant pain as early as 6 weeks after injury. Although neuropathic pain is a common consequence after CNS injuries, little attention has been given to neuropathic pain symptoms after...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Widerström-Noga E,Govind V,Adcock JP,Levin BE,Maudsley AA

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  • Mode changes of cerebrovascular pressure transmission induced by cerebral vasodilation.

    abstract::Changes in the modes of cerebrovascular pressure transmission during cerebral vasodilation induced by hypercapnic challenge were examined as a means for developing the basis for a bedside method to evaluate regulation of cerebral blood flow. Recordings of arterial blood pressure (ABP) and intracranial pressure (ICP) o...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Daley ML,Pourcyrous M,Timmons SD,Leffler CW

    更新日期:2007-03-01 00:00:00

  • Comparing model performance for survival prediction using total Glasgow Coma Scale and its components in traumatic brain injury.

    abstract::The Glasgow Coma Scale (GCS) score is used in clinical practice for patient assessment and communication among clinicians and also in outcome prediction models such as the Trauma and Injury Severity Score (TRIS). The objective of this study is to determine which GCS subscore is best associated with outcome, taking tim...

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    pub_type: 杂志文章


    authors: Lesko MM,Jenks T,O'Brien SJ,Childs C,Bouamra O,Woodford M,Lecky F

    更新日期:2013-01-01 00:00:00

  • Effect of posttraumatic hyperglycemia on contusion volume and neutrophil accumulation after moderate fluid-percussion brain injury in rats.

    abstract::The purpose of this study was to evaluate the effects of posttraumatic hyperglycemia on contusion volume and neutrophil accumulation following moderate traumatic brain injury (TBI) in rats. A parasagittal fluid-percussion (F-P) brain injury (1.8-2.1 atm) was induced in male Sprague-Dawley rats. Rats were then randomiz...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Kinoshita K,Kraydieh S,Alonso O,Hayashi N,Dietrich WD

    更新日期:2002-06-01 00:00:00

  • Temporal effect of severe controlled cortical impact injury in the rat on the myogenic response of the middle cerebral artery.

    abstract::The present study examined the effect of severe traumatic brain injury (TBI) on the myogenic response in the rat middle cerebral artery (MCA). Rats were subjected to severe controlled cortical impact (CCI; 5 m/sec, 130-msec duration, 3-mm deformation) injury over the right parietal cortex. At 2, 24, and 120 h postinju...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Golding EM,Contant CF Jr,Robertson CS,Bryan RM Jr

    更新日期:1998-11-01 00:00:00

  • tPA-S481A prevents neurotoxicity of endogenous tPA in traumatic brain injury.

    abstract::Traumatic brain injury (TBI) is associated with loss of autoregulation due to impaired responsiveness to cerebrovascular dilator stimuli, which leads to cerebral hypoperfusion and neuronal impairment or death. Upregulation of tissue plasminogen activator (tPA) post-TBI exacerbates loss of cerebral autoregulation and N...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Armstead WM,Riley J,Yarovoi S,Cines DB,Smith DH,Higazi AA

    更新日期:2012-06-10 00:00:00

  • Serum biomarker concentrations and outcome after pediatric traumatic brain injury.

    abstract::Predicting outcome after pediatric traumatic brain injury (TBI) is important for providing information to families and prescribing rehabilitation services. The study objective was to assess whether biomarkers concentrations obtained at the time of injury are associated with outcome. Serial serum concentrations of neur...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Berger RP,Beers SR,Richichi R,Wiesman D,Adelson PD

    更新日期:2007-12-01 00:00:00

  • The effects of repeat traumatic brain injury on the pituitary in adolescent rats.

    abstract::Adolescents are one of the highest groups at risk for sustaining both traumatic brain injury (TBI) and repeat TBI (RTBI). Consequences of endocrine dysfunction following TBI have been routinely explored in adults, but studies in adolescents are limited, and show an incidence rate of endocrine dysfunction in 16-61% in ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Greco T,Hovda D,Prins M

    更新日期:2013-12-01 00:00:00

  • Restraints and peripheral nerve injuries in adult victims of motor vehicle crashes.

    abstract::The pattern of injuries in restrained victims of motor vehicle crashes (MVCs) remains an issue of debate. We investigated the association of peripheral nerve injuries with the use of protective devices (seat belt and air bag) during MVCs. We performed a retrospective cohort study of 384,539 adult MVC victims who were ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Bekelis K,Missios S,Spinner RJ

    更新日期:2014-06-15 00:00:00

  • Experimental traumatic brain injury induces a pervasive hyperanxious phenotype in rats.

    abstract::Mood disturbances, including depression and anxiety disorders, are common and disabling long-term sequelae of traumatic brain injury (TBI). These psychiatric conditions have generally been considered psychosocial consequences of the trauma, but neurobiological alterations and causes have also been implicated. Using a ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Jones NC,Cardamone L,Williams JP,Salzberg MR,Myers D,O'Brien TJ

    更新日期:2008-11-01 00:00:00

  • Beneficial Effects of Kaempferol after Developmental Traumatic Brain Injury Is through Protection of Mitochondrial Function, Oxidative Metabolism, and Neural Viability.

    abstract::Oxidative energy metabolism is depressed after mild/moderate traumatic brain injury (TBI) during early development, accompanied by behavioral debilitation and secondary neuronal death. A TBI metabolome analysis revealed broad effects with a striking impact on energy metabolism. Our studies on mitochondrial modulators ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Chitturi J,Santhakumar V,Kannurpatti SS

    更新日期:2019-04-15 00:00:00

  • Multiple Mild Traumatic Brain Injuries Lead to Visual Dysfunction in a Mouse Model.

    abstract::Visual dysfunction is a common occurrence after traumatic brain injury (TBI). We investigated in this study effects of single or multiple mild TBI on visual function in mice using a closed head injury model that permits unconstrained head movement after impact. Adult mice were briefly anesthetized with isoflurane and ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Desai A,Chen H,Kim HY

    更新日期:2020-01-15 00:00:00

  • Risk of Depression after Traumatic Brain Injury in a Large National Sample.

    abstract::Depression is associated with poorer recovery after traumatic brain injury (TBI), yet awareness of depression risk post-TBI among providers and patients is low. The aim of this study was to estimate risk of depression post-TBI among adults 18 years of age and older and to identify risk factors associated with developi...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Albrecht JS,Barbour L,Abariga SA,Rao V,Perfetto EM

    更新日期:2019-01-15 00:00:00

  • Frontal cortex neuropathology in dementia pugilistica.

    abstract::Dementia pugilistica (DP) is associated with chronic traumatic brain injury (CTBI), and leads to a "punch drunk" syndrome characterized by impairments in memory and executive function, behavioral changes, and motor signs. Microscopic features include the accumulation of neurofibrillary tangles (NFTs), beta-amyloid (Aβ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Saing T,Dick M,Nelson PT,Kim RC,Cribbs DH,Head E

    更新日期:2012-04-10 00:00:00

  • The Autonomic System Functional State Predicts Responsiveness in Disorder of Consciousness.

    abstract::Diagnosis and early prognosis of the vegetative state/unresponsive wakefulness syndrome (VS/UWS) and its differentiation from the minimally-conscious state still rest on the clinical observation of responsiveness. The incidence of established clinical indicators of responsiveness also has proven variable in the single...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Riganello F,Cortese MD,Dolce G,Lucca LF,Sannita WG

    更新日期:2015-07-15 00:00:00

  • Brain Networks Subserving Emotion Regulation and Adaptation after Mild Traumatic Brain Injury.

    abstract::The majority of patients with traumatic brain injury (TBI) sustain a mild injury (mTBI). One out of 4 patients experiences persistent complaints, despite their often normal neuropsychological test results and the absence of structural brain damage on conventional neuroimaging. Susceptibility to develop persistent comp...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章,评审


    authors: van der Horn HJ,Liemburg EJ,Aleman A,Spikman JM,van der Naalt J

    更新日期:2016-01-01 00:00:00

  • Relative risk of mortality after traumatic brain injury: a population-based study of the role of age and injury severity.

    abstract::To test if observed vs. expected mortality differs by age among traumatic brain injury (TBI) cases, a population-based, historical cohort study was conducted in Olmsted County, Minnesota. From all residents with any diagnosis suggestive of TBI 1985-1999, we randomly sampled 7,800 and reviewed their medical records to ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Flaada JT,Leibson CL,Mandrekar JN,Diehl N,Perkins PK,Brown AW,Malec JF

    更新日期:2007-03-01 00:00:00

  • Transcriptional Changes in the Mouse Retina after Ocular Blast Injury: A Role for the Immune System.

    abstract::Ocular blast injury is a major medical concern for soldiers and explosion victims due to poor visual outcomes. To define the changes in gene expression following a blast injury to the eye, we examined retinal ribonucleic acid (RNA) expression in 54 mouse strains 5 days after a single 50-psi overpressure air wave blast...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Struebing FL,King R,Li Y,Chrenek MA,Lyuboslavsky PN,Sidhu CS,Iuvone PM,Geisert EE

    更新日期:2018-01-01 00:00:00

  • A follow-up study of neurometabolic alterations in female concussed athletes.

    abstract::Athletes who sustain a concussion demonstrate a variety of symptoms and neuropsychological alterations that could be brought on by neurometabolic abnormalities. However, no study has yet investigated these aspects in female athletes using magnetic resonance spectroscopy. The present study investigated the neurometabol...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Chamard E,Henry L,Boulanger Y,Lassonde M,Théoret H

    更新日期:2014-02-15 00:00:00

  • Validation of a Novel Clinical Score: The Rostock Functional and Cosmetic Cranioplasty Score.

    abstract::With a rising number of cranioplasty (CP) procedures and an increasing percentage of patients with a good clinical outcome and prolonged survival after CP, looking at the functional and aesthetic outcome of these patients becomes more and more important. The aim of our study was to evaluate a novel score, combining fu...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Henker C,Hoppmann MC,Sherman MUS,Glass A,Piek J

    更新日期:2018-04-15 00:00:00

  • Development of a database for translational spinal cord injury research.

    abstract::Efforts to understand spinal cord injury (SCI) and other complex neurotrauma disorders at the pre-clinical level have shown progress in recent years. However, successful translation of basic research into clinical practice has been slow, partly because of the large, heterogeneous data sets involved. In this sense, tra...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Nielson JL,Guandique CF,Liu AW,Burke DA,Lash AT,Moseanko R,Hawbecker S,Strand SC,Zdunowski S,Irvine KA,Brock JH,Nout-Lomas YS,Gensel JC,Anderson KD,Segal MR,Rosenzweig ES,Magnuson DS,Whittemore SR,McTigue DM,Popovic

    更新日期:2014-11-01 00:00:00

  • Glial cell-mediated deterioration and repair of the nervous system after traumatic brain injury in a rat model as assessed by positron emission tomography.

    abstract::Traumatic brain injury (TBI) is one of the most acute degenerative pathologies in the central nervous system, and in vivo indices enabling an assessment of TBI on a mechanistic basis have yet to be established. The aim of this work was to pursue neuroinflammatory changes and their link to functional disruptions of tra...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Yu I,Inaji M,Maeda J,Okauchi T,Nariai T,Ohno K,Higuchi M,Suhara T

    更新日期:2010-08-01 00:00:00

  • Neuronal and glial cell number in the hippocampus after experimental traumatic brain injury: analysis by stereological estimation.

    abstract::Fluid percussion (FP) brain injury causes spatial memory dysfunction in rats regardless of injury location (midline vs. lateral). Standard histological analysis of the injured brains shows hippocampal neuronal loss after lateral, but not midline FP injury. We have used the optical volume fractionator (OVF) stereologic...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Grady MS,Charleston JS,Maris D,Witgen BM,Lifshitz J

    更新日期:2003-10-01 00:00:00

  • Resting-State Magnetoencephalography Source Imaging Pilot Study in Children with Mild Traumatic Brain Injury.

    abstract::Mild traumatic brain injury (mTBI) accounts for the vast majority of all pediatric TBI. An important minority of children who have suffered an mTBI have enduring cognitive and emotional symptoms. However, the mechanisms of chronic symptoms in children with pediatric mTBI are not fully understood. This is in part due t...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Huang MX,Robb Swan A,Angeles Quinto A,Huang JW,De-la-Garza BG,Huang CW,Hesselink JR,Bigler ED,Wilde EA,Max JE

    更新日期:2020-04-01 00:00:00

  • Resuscitation with Lyophilized Plasma Is Safe and Improves Neurological Recovery in a Long-Term Survival Model of Swine Subjected to Traumatic Brain Injury, Hemorrhagic Shock, and Polytrauma.

    abstract::We have shown previously that fresh frozen plasma (FFP) and lyophilized plasma (LP) decrease brain lesion size and improve neurological recovery in a swine model of traumatic brain injury (TBI) and hemorrhagic shock (HS). In this study, we examine whether these findings can be validated in a clinically relevant model ...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Georgoff PE,Nikolian VC,Halaweish I,Chtraklin K,Bruhn PJ,Eidy H,Rasmussen M,Li Y,Srinivasan A,Alam HB

    更新日期:2017-07-01 00:00:00

  • Intracerebroventricular transplantation of ex vivo expanded endothelial colony-forming cells restores blood-brain barrier integrity and promotes angiogenesis of mice with traumatic brain injury.

    abstract::Endothelial progenitor cells (EPCs) play a key role in tissue repair and regeneration. Previous studies have shown a positive correlation between the number of circulating EPCs and clinical outcomes of patients with traumatic brain injury (TBI). A recent study has further shown that intravenous infusion of human umbil...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Huang XT,Zhang YQ,Li SJ,Li SH,Tang Q,Wang ZT,Dong JF,Zhang JN

    更新日期:2013-12-15 00:00:00

  • Electrophysiological and synaptic characterization of transplanted neurons in adult rat motor cortex.

    abstract::Lesions in specific areas of the rat motor cortex generate deficits related to fine movement performance affecting the forelimb. We have previously shown that transplants of embryonic frontal cortex ameliorate these motor deficits. Amelioration has been associated with a functional integration of the transplant due to...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Santos-Torres J,Heredia M,Riolobos AS,Jiménez-Díaz L,Gómez-Bautista V,de la Fuente A,Criado JM,Navarro-López J,Yajeya J

    更新日期:2009-09-01 00:00:00

  • Mechanical stretch to neurons results in a strain rate and magnitude-dependent increase in plasma membrane permeability.

    abstract::The mechanism by which mechanical impact to brain tissue is transduced to neuronal impairment remains poorly understood. Using an in vitro model of neuronal stretch, we found that mechanical stretch of neurons resulted in a transient plasma membrane permeability increase. Primary cortical neurons, seeded on silicone s...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Geddes DM,Cargill RS 2nd,LaPlaca MC

    更新日期:2003-10-01 00:00:00

  • Cardiometabolic risk factors in experimental spinal cord injury.

    abstract::Cardiometabolic risk factors are sorely underreported after spinal cord injury (SCI), despite the high prevalence of metabolic disorders and cardiovascular mortality in this population. Body-composition analysis and serum-lipid profiling are two assessments that are beginning to be more widely used to document metabol...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Inskip J,Plunet W,Ramer L,Ramsey JB,Yung A,Kozlowski P,Ramer M,Krassioukov A

    更新日期:2010-01-01 00:00:00

  • Platelet dysfunction in patients with severe traumatic brain injury.

    abstract::Coagulopathy is a common phenomenon in traumatic brain injury (TBI) and a major contributor to a poor outcome. Thrombocytopenia is a strong negative prognostic factor in TBI, but bleeding tendency can be present even with a normal platelet count. We investigated platelet function in patients with TBI by means of modif...

    journal_title:Journal of neurotrauma

    pub_type: 杂志文章


    authors: Nekludov M,Bellander BM,Blombäck M,Wallen HN

    更新日期:2007-11-01 00:00:00