Plaque purification as a method to mitigate the risk of adventitious-agent contamination in influenza vaccine virus seeds.


:At present, the seed viruses for the manufacture of licensed seasonal inactivated influenza vaccines in the United States are derived from primary egg isolates as a result of concerns associated with adventitious agents. According to the prevailing view, the passage of influenza viruses through eggs serves as a filtering step to remove potential contaminating viruses. We have investigated the feasibility of addressing adventitious-agent risk by subjecting influenza virus to a plaque-purification procedure using MDCK cells. SV40 and canine adenovirus-1 (representing viruses for which MDCK cells are non-permissive and permissive, respectively) were used as challenge viruses to model agents of concern that might be co-isolated along with the influenza virus. By mixing influenza virus strain A/PR/8/34 with varying amounts of each challenge virus and then performing a plaque assay for influenza virus using MDCK cells, we have attempted to determine the efficiency by which the challenge virus is removed. Our data suggest that substantial removal can be achieved even after a single round of plaque purification. If cell-derived isolates were deemed to be acceptable following a plaque-purification procedure, the manufacture of seasonal influenza vaccine would be facilitated by: (1) the expansion of the repertoire of viruses from which seed virus candidates could be generated for licensed egg-derived vaccines as well as for vaccines manufactured in mammalian cells; and (2) the mitigation of adventitious-agent risk associated with the seed virus, and hence the elimination of the need to passage seed viruses in eggs for vaccines manufactured in mammalian cells.






Murata H,Macauley J,Lewis AM Jr,Peden K




Has Abstract


2011-04-12 00:00:00
















  • Cost of plasma-derived hepatitis B vaccine production.

    abstract::It is generally accepted that the introduction of hepatitis B vaccine to national programmes of immunization in developing countries is desirable, but it has so far been constrained by the high cost of the vaccine. To determine the potential minimal costs of the vaccine, a study has been carried out of the production ...


    pub_type: 杂志文章


    authors: Mahoney RT

    更新日期:1990-08-01 00:00:00

  • Protection against experimental bubonic and pneumonic plague by a recombinant capsular F1-V antigen fusion protein vaccine.

    abstract::The current human whole-cell vaccine is ineffective against pneumonic plague caused by typical F1 capsule positive (F1+) strains of Yersinia pestis. The authors found this vaccine to also be ineffective against F1-negative (F1-) Y. pestis strains, which have been isolated from a human case and from rodents. For these ...


    pub_type: 杂志文章


    authors: Heath DG,Anderson GW Jr,Mauro JM,Welkos SL,Andrews GP,Adamovicz J,Friedlander AM

    更新日期:1998-07-01 00:00:00

  • Immunopotentiating heat shock proteins: negotiators between innate danger and control of autoimmunity.

    abstract::Heat shock proteins (hsps) are known to be immunodominant antigens of bacteria. Hsps are evolutionarily strongly conserved proteins present in all eukaryotic and prokaryotic cellular organisms and upregulated by several forms of stress. Despite (the paradigm of) self-tolerance, hsp-epitopes homologous to endogenous ho...


    pub_type: 杂志文章,评审


    authors: van Eden W,Koets A,van Kooten P,Prakken B,van der Zee R

    更新日期:2003-02-14 00:00:00

  • Isolation and characterization of influenza C virus inhibitors in rat serum.

    abstract::Two inhibitors against haemagglutination by influenza C virus were isolated from pooled sera of normal rats by sequential chromatography on Blue Sepharose CL 6B, Ultrogel AcA 2, and DEAE-cellulose. The two inhibitors were identified as alpha 1-macroglobulin and murinoglobulin by comparison with the authentic samples. ...


    pub_type: 杂志文章


    authors: Kitame F,Nakamura K,Saito A,Sinohara H,Homma M

    更新日期:1985-09-01 00:00:00

  • Pneumococcal pneumonia in the UK--how herd immunity affects the cost-effectiveness of 7-valent pneumococcal conjugate vaccine (PCV).

    abstract::This study examines the potential clinical and economic benefits for adults arising from paediatric pneumococcal vaccination in the UK. A UK birth cohort model with a 10-year horizon and using a primary 4-dose paediatric 7-valent pneumococcal conjugate vaccine (PCV) schedule was populated with 1999 morbidity and morta...


    pub_type: 杂志文章


    authors: McIntosh ED,Conway P,Willingham J,Hollingsworth R,Lloyd A

    更新日期:2005-02-25 00:00:00

  • Targeting subcapsular antigens for prevention of Klebsiella pneumoniae infections.

    abstract::Vaccination strategies against Klebsiella pneumoniae have largely focussed on the polysaccharide capsule. However, the large number and high prevalence of individual capsular serotypes limits the widespread applicability of capsule-based vaccines. This study establishes that immunization with purified LPS can protect ...


    pub_type: 杂志文章


    authors: Clements A,Jenney AW,Farn JL,Brown LE,Deliyannis G,Hartland EL,Pearse MJ,Maloney MB,Wesselingh SL,Wijburg OL,Strugnell RA

    更新日期:2008-10-16 00:00:00

  • Effect of immunization with herpes simplex virus type-1 (HSV-1) glycoprotein D (gD) plus the immune enhancer GPI-0100 on infection with HSV-1 or HSV-2.

    abstract::These studies were performed to determine the effects of GPI-0100, a semi synthetic Quillaja Saponin analog, formulated with herpes simplex virus type-1 (HSV-1) glycoprotein D (gD) on immunity to HSV. SKH-1 hairless mice, used as a model of herpes labialis, inoculated with HSV-1 results in facial lesions, virus replic...


    pub_type: 杂志文章


    authors: Quenelle DC,Collins DJ,Marciani DJ,Kern ER

    更新日期:2006-03-06 00:00:00

  • HIV vaccine candidates generate in vitro T cell response to putative epitopes in Chinese-origin rhesus macaques.

    abstract::The Indian rhesus macaque is the established animal model for HIV infection and vaccine research. Growing evidence suggests that the more readily available Chinese rhesus macaque may be a more relevant option. As increasing numbers of novel Chinese rhesus MHC alleles are reported, we decided to explore potential HIV v...


    pub_type: 杂志文章


    authors: Liao Q,Strong AJ,Liu Y,Liu Y,Meng P,Fu Y,Touzjian N,Shao Y,Zhao Z,Lu Y

    更新日期:2012-02-21 00:00:00

  • Exploratory efficacy endpoints in the Community-Acquired Pneumonia Immunization Trial in Adults (CAPiTA).

    abstract:BACKGROUND:The Community-Acquired Pneumonia Immunization Trial in Adults (CAPiTA) assessed vaccine-type community-acquired pneumonia (VT-CAP) and vaccine-type invasive pneumococcal disease (VT-IPD) prevention with 13-valent pneumococcal conjugate vaccine (PCV13) in adults aged ⩾65years. We report vaccine efficacy (VE) ...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Webber C,Patton M,Patterson S,Schmoele-Thoma B,Huijts SM,Bonten MJ,CAPiTA Study Group.

    更新日期:2017-03-01 00:00:00

  • Safety of yellow fever vaccine administration in confirmed egg-allergic patients.

    abstract::Yellow fever vaccine (YFV) is recommended in endemic areas but represents a risk for egg-allergic patients, as it is cultivated in embryonated eggs. This study aims to describe the outcomes of yellow fever vaccination in patients with confirmed egg allergy (EA). Methods:A prospective study was conducted from January 2...


    pub_type: 杂志文章


    authors: Gerhardt CMB,Castro APBM,Pastorino AC,Dorna MB,Nunes-Santos CJ,Aquilante BP,Miyaji KT,Lopes MH

    更新日期:2020-09-29 00:00:00

  • Characterisation of a protective linear B cell epitope against feline parvoviruses.

    abstract::Monoclonal antibody 3C9 was the starting material in the definition of the epitope that led to the synthesis of the first efficient peptide vaccine against a viral disease (canine parvovirus) in the natural host (dog). In this report, we have analysed the specificity of the antibody at the single amino acid level and ...


    pub_type: 杂志文章


    authors: Langeveld JP,Martinez-Torrecuadrada J,Boshuizen RS,Meloen RH,Ignacio Casal J

    更新日期:2001-03-21 00:00:00

  • Vaccination with a gE-negative bovine herpesvirus type 1 vaccine confers insufficient protection to a bovine herpesvirus type 5 challenge.

    abstract::In the present study, cross-protection to bovine herpesvirus type 5 (BHV-5) induced by bovine herpesvirus type 1 (BHV-1) vaccination was examined following inoculation of rabbits and calves with a glycoprotein E (gE)-negative BHV-1 vaccine and subsequent challenge with BHV-5. Rabbits (n=5) and calves (n=8) were vaccin...


    pub_type: 杂志文章


    authors: Silva AD,Spilki FR,Franco AC,Esteves PA,Hübner SO,Driemeier D,Oliveira AP,Rijsewijk F,Roehe PM

    更新日期:2006-04-12 00:00:00

  • Recombinant retrovirus-derived virus-like particle-based vaccines induce hepatitis C virus-specific cellular and neutralizing immune responses in mice.

    abstract::While the immunological correlates of hepatitis C virus (HCV)-specific immunity are not well understood, it is now admitted that an effective vaccine against HCV will need to induce both cellular and humoral immune responses and address viral heterogeneity to prevent immune escape. We developed a vaccine platform spec...


    pub_type: 杂志文章


    authors: Huret C,Desjardins D,Miyalou M,Levacher B,Amadoudji Zin M,Bonduelle O,Combadière B,Dalba C,Klatzmann D,Bellier B

    更新日期:2013-03-01 00:00:00

  • Toxicity assessment of Clostridium difficile toxins in rodent models and protection of vaccination.

    abstract::Clostridium difficile is the leading cause of hospital-acquired diarrhea, also known as C. difficile associated diarrhea. The two major toxins, toxin A and toxin B are produced by most C. difficile bacteria, but some strains, such as BI/NAP1/027 isolates, produce a third toxin called binary toxin. The precise biologic...


    pub_type: 杂志文章


    authors: Wang S,Rustandi RR,Lancaster C,Hong LG,Thiriot DS,Xie J,Secore S,Kristopeit A,Wang SC,Heinrichs JH

    更新日期:2016-03-04 00:00:00

  • Detection of measles vaccine in the throat of a vaccinated child.

    abstract::Measles vaccine is widely used, most often in association with mumps and rubella vaccines. We report here the case of a child presenting with fever 8 days after vaccination with a measles-mumps-rubella vaccine. Measles virus was isolated in a throat swab taken 4 days after fever onset. This virus was then further gene...


    pub_type: 杂志文章


    authors: Morfin F,Beguin A,Lina B,Thouvenot D

    更新日期:2002-02-22 00:00:00

  • A bivalent vaccine to protect against Streptococcus pneumoniae and Salmonella typhi.

    abstract::Pneumococcal and Salmonella typhi infections are two major diseases for children in developing countries. For typhoid fever, licensed Vi polysaccharide vaccines are ineffective in children <2-year old. While investigational Vi conjugate vaccines have been shown effective in clinical trials, they are currently only ava...


    pub_type: 杂志文章


    authors: Lu YJ,Zhang F,Sayeed S,Thompson CM,Szu S,Anderson PW,Malley R

    更新日期:2012-05-14 00:00:00

  • C3d enhancement of neutralizing antibodies to measles hemagglutinin.

    abstract::Measles remains a major cause of worldwide infant mortality despite the use of current live attenuated vaccines. New approaches to measles virus (MV) vaccine development are critical to interrupt the spread of MV. In this study, we report the results using a DNA vaccine expressing a fusion of the measles hemagglutinin...


    pub_type: 杂志文章


    authors: Green TD,Newton BR,Rota PA,Xu Y,Robinson HL,Ross TM

    更新日期:2001-10-12 00:00:00

  • Cost-effectiveness of three different vaccination strategies against measles in Zambian children.

    abstract::The vaccination program in Zambia includes one dose of measles vaccine at 9 months of age. The objective of this study was to compare the cost-effectiveness of the current one-dose measles vaccination program with an immunization schedule in which a second dose is provided either through routine health services or thr...


    pub_type: 杂志文章


    authors: Dayan GH,Cairns L,Sangrujee N,Mtonga A,Nguyen V,Strebel P

    更新日期:2004-01-02 00:00:00

  • Analysis of PorA variable region 3 in meningococci: implications for vaccine policy?

    abstract::Outer membrane protein (OMP) vaccines are being developed against Neisseria meningitidis serogroup B which may provide protection against common circulating serotypes and serosubtypes in some countries. However, limited data is available in Europe from genosubtyping meningococci. We therefore undertook a retrospective...


    pub_type: 杂志文章


    authors: Clarke SC,Diggle MA,Mölling P,Unemo M,Olcén P

    更新日期:2003-06-02 00:00:00

  • Size determination of bacterial capsular oligosaccharides used to prepare conjugate vaccines.

    abstract::We recently described the use of ion exchange chromatography for analysis and the industrial scale preparation of pools of oligosaccharides of intermediate chain length from polysaccharides of Haemophilus influenzae type b (Hib) and Neisseria meningitidis groups A and C. These negatively charged "sized" oligosaccharid...


    pub_type: 杂志文章


    authors: Ravenscroft N,Averani G,Bartoloni A,Berti S,Bigio M,Carinci V,Costantino P,D'Ascenzi S,Giannozzi A,Norelli F,Pennatini C,Proietti D,Ceccarini C,Cescutti P

    更新日期:1999-07-16 00:00:00

  • The use of sequence analysis of a feline calicivirus (FCV) hypervariable region in the epidemiological investigation of FCV related disease and vaccine failures.

    abstract::A reverse transcriptase polymerase chain reaction (PCR) was used to amplify a 235 bp hypervariable region of the feline calicivirus (FCV) genome which encodes part of the capsid protein. Sequence from this region was used to compare viruses used in three attenuated vaccines to viruses isolated from vaccinated cats wit...


    pub_type: 杂志文章


    authors: Radford AD,Bennett M,McArdle F,Dawson S,Turner PC,Glenn MA,Gaskell RM

    更新日期:1997-08-01 00:00:00

  • Evaluation of a single dose of half strength inactivated influenza vaccine in healthy adults.

    abstract::Because of delays in the manufacturing of the 2000-2001, trivalent inactivated influenza vaccine in the US, there were concerns that there might be shortages of vaccine supply in the US. Therefore, we conducted a prospective, randomized, open-label, multicenter trial at six academic medical centers in the US, to evalu...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Treanor J,Keitel W,Belshe R,Campbell J,Schiff G,Zangwill K,Wolff M,Klimov A,Levandowski R,Lambert L

    更新日期:2002-01-15 00:00:00

  • The effect of early measles vaccination on thymic size. A randomized study from Guinea-Bissau.

    abstract:UNLABELLED:In low-income countries early measles vaccine (MV) is associated with reduced child mortality which cannot be explained by prevention of measles. A large thymus gland in infancy is also associated with reduced mortality. We hypothesized that early MV is associated with increased thymic size. Within a randomi...


    pub_type: 杂志文章,随机对照试验


    authors: Christensen LD,Eriksen HB,Biering-Sørensen S,Balé C,Do VA,Andersen A,Martins CL,Sodemann M,Aaby P,Benn CS

    更新日期:2014-03-26 00:00:00

  • Immune responses to a GnRH-based anti-fertility immunogen, induced by different adjuvants and subsequent effect on vaccine efficacy.

    abstract::A modified GnRH peptide (CHWSYGLRPG-NH2) was conjugated to tetanus toxoid and formulated with different adjuvants (non-ionic surfactant vesicles, aluminium hydroxide, Quil A, PLGA (poly(lactide-co-glycolide)/triacetin), and Quil A/PLGA). A comparison of the anti-fertility efficacy of the formulations was made by exami...


    pub_type: 杂志文章


    authors: Ferro VA,Costa R,Carter KC,Harvey MJ,Waterston MM,Mullen AB,Matschke C,Mann JF,Colston A,Stimson WH

    更新日期:2004-02-25 00:00:00

  • Economic benefits for the family of inactivated subunit virosomal influenza vaccination of healthy children aged 3-14 years during the annual health examination in private paediatric offices.

    abstract::Taking the results of a prospective cohort study by our group that evaluated the effectiveness of the inactivated subunit virosomal influenza vaccine (Inflexal V), Crucell-Berna) in the prevention of influenza-related diseases and the reduction of its negative economic consequences, the economic costs and benefits for...


    pub_type: 杂志文章


    authors: Salleras L,Navas E,Domínguez A,Ibáñez D,Prat A,Garrido P,Asenjo MA,Torner N

    更新日期:2009-05-26 00:00:00

  • Use of a vaccinia-rabies recombinant virus for the oral vaccination of foxes against rabies.

    abstract::The vaccination of wild animals against rabies has been developed most extensively in Europe. Experiments have demonstrated the efficacy of a vaccinia-rabies recombinant virus administered by the oral route in foxes. The innocuity of this vaccine was tested in the target species as well as in several non-target wild a...


    pub_type: 杂志文章


    authors: Brochier B,Thomas I,Bauduin B,Leveau T,Pastoret PP,Languet B,Chappuis G,Desmettre P,Blancou J,Artois M

    更新日期:1990-04-01 00:00:00

  • Evaluation of a Salmonella Enteritidis vaccine and related ELISA for respective induction and assessment of acquired immunity to the vaccine and/or Echinacea purpurea in Awassi Ewes.

    abstract::The aim of this study was to evaluate an experimental Salmonella Enteritidis (SE) bacterin and an indirect ELISA system to assess quantitatively the acquired immunity in Awassi ewes to the vaccine and/or Echinacea purpurea (EP) dried roots. Four treatments of the ewes were included in the experimental design, with 6 e...


    pub_type: 杂志文章


    authors: Barbour EK,Assi CAA,Shaib H,Hamadeh S,Murtada M,Mahmoud G,Yaghmoor S,Iyer A,Harakeh S,Kumosani T

    更新日期:2015-05-05 00:00:00

  • Evaluation of the immunogenicity and protective efficacy of a candidate parainfluenza virus type 3 subunit vaccine in cotton rats.

    abstract::A parainfluenza virus type 3 (PIV3) subunit vaccine consisting of detergent-solubilized, affinity-purified haemagglutinin-neuraminidase (HN) and fusion (F) surface glycoproteins was tested in cotton rats for immunogenicity, short-term effects on virus-induced immunopathology and protective efficacy. Groups of animals ...


    pub_type: 杂志文章


    authors: Ambrose MW,Wyde PR,Ewasyshyn M,Bonneau AM,Caplan B,Meyer HL,Klein M

    更新日期:1991-07-01 00:00:00

  • Aluminum salts in vaccines--US perspective.

    abstract::Aluminum in the form of aluminum hydroxide, aluminum phosphate or alum has been commonly used as an adjuvant in many vaccines licensed by the US Food and Drug Administration. Chapter 21 of the US Code of Federal Regulations [610.15(a)] limits the amount of aluminum in biological products, including vaccines, to 0.85 m...


    pub_type: 杂志文章,评审


    authors: Baylor NW,Egan W,Richman P

    更新日期:2002-05-31 00:00:00

  • Plasmodium falciparum serine repeat antigen 5 (SE36) as a malaria vaccine candidate.

    abstract::A devastating disease spread by mosquitoes with high-efficiency, malaria imposes an enormous burden for which no licensed vaccine currently exists. Although the genome complexity of the parasite has made vaccine development tenuous, an effective malaria vaccine would be a valuable tool for control, elimination and eve...


    pub_type: 杂志文章,评审


    authors: Palacpac NM,Arisue N,Tougan T,Ishii KJ,Horii T

    更新日期:2011-08-11 00:00:00