Salmonella Enteritidis with double deletion in phoPfliC--a potential live Salmonella vaccine candidate with novel characteristics for use in chickens.


:Salmonella Enteritidis mutants with deletions in phoP, fliC or phoPfliC were tested for their virulence and their ability to induce parameters of the innate and adaptive immunity in addition to their potential for serological differentiation between vaccinated, non-vaccinated and infected chickens. The double phoPfliC deletion mutant was sufficiently attenuated but not diminished in its capability to inhibit the caecal colonisation and systemic invasion of homologous Salmonella Enteritidis shortly after administration of the vaccine strain to very young chicks. Immunisation with the attenuated ΔphoPfliC mutant resulted in protective effects which were only slightly and insignificantly lower than after "immunisation" with a Salmonella wild-type strain, indicating the capability to induce an intense adaptive immune response and protection against Salmonella exposure in older chickens. The deletion in fliC enabled the effective the differentiation between immunised and infected chickens using a commercially available ELISA kit. The double phoPfliC deletion mutant of Salmonella Enteritidis might be a potential and promising live Salmonella vaccine candidate with novel characteristics for use in poultry.






Methner U,Barrow PA,Berndt A,Rychlik I




Has Abstract


2011-04-12 00:00:00
















  • Use of o-phthalaldehyde assay to determine protein contents of Alhydrogel-based vaccines.

    abstract::Aluminum based adjuvants (alum), including aluminum hydroxide (Alhydrogel) and aluminum phosphate are the most commonly used adjuvant in the US. In order to ensure quality of vaccines, regulatory authorities require evaluation of antigen content in final vaccine products. Currently, there are no generic methods availa...


    pub_type: 杂志文章


    authors: Zhu D,Saul A,Huang S,Martin LB,Miller LH,Rausch KM

    更新日期:2009-10-09 00:00:00

  • High immunogenicity of delayed third dose of hepatitis B vaccine in travellers.

    abstract::Travellers often present late for the third dose of hepatitis B vaccination and measuring anti-HBs serology is common practice to ensure that these individuals are protected. We assessed the adequacy of immune response in 86 healthy travellers who had their third dose of hepatitis B (HB) vaccine performed more than 1 ...


    pub_type: 杂志文章


    authors: Jackson Y,Chappuis F,Mezger N,Kanappa K,Loutan L

    更新日期:2007-04-30 00:00:00

  • Safety and immunogenicity of a hepatitis B vaccine formulated with a novel adjuvant system.

    abstract::A formulation of recombinant hepatitis B surface antigen (HBsAg) combined with a novel adjuvant system, SBAS4--a combination of aluminium salt and monophosphoryl lipid A (MPL), was assessed in 27 healthy adult volunteers with a commercial vaccine (Engerix-B) as control. After three doses (0, 1, 6 months schedule), rea...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Thoelen S,Van Damme P,Mathei C,Leroux-Roels G,Desombere I,Safary A,Vandepapeliere P,Slaoui M,Meheus A

    更新日期:1998-04-01 00:00:00

  • Influenza virus antigens conjugated with a synthetic polyelectrolyte: a novel model of vaccines.

    abstract::Haemagglutinin (HA), a mixture of haemagglutinin and neuraminidase (HA + NA), and matrix (M) protein were isolated from the influenza A virus and covalently coupled to a synthetic polyelectrolyte (P). A single injection into mice of the resultant conjugates (virogates) brought about efficient stimulation of the primar...


    pub_type: 杂志文章


    authors: Petrov RV,Khaitov RM,Zhdanov VM,Sinyakov MS,Norimov ASh,Nekrasov AV,Podchernyaeva RYa,Kharitonenkov IG,Shchipanova MV

    更新日期:1985-12-01 00:00:00

  • Choice of measures of vaccination and estimates of risk of pediatric pertussis.

    abstract:BACKGROUND:Vaccination uptake at the individual level can be assessed in a variety of ways, including traditional measures of being up-to-date (UTD), measures of UTD that consider dose timing, like age-appropriate vaccination, and risk reduction from individual doses. This analysis compared methods of operationalizing ...


    pub_type: 杂志文章


    authors: Goldstein ND,Newbern EC,Evans AA,Drezner K,Welles SL

    更新日期:2015-07-31 00:00:00

  • Plant heat shock protein 90 as carrier-adjuvant for immunization against a reporter antigen.

    abstract::Here, we evaluated the modulation of the immune response induced by Hsp90 of Nicotiana benthamiana (NbHsp90.3) against the Maltose Binding Protein (MBP) as a reporter antigen. Equimolar quantities of recombinant proteins were administered in mice as follows: MBP alone (MBP group), a mixture of MBP and rNbHsp90.3 (MBP+...


    pub_type: 杂志文章


    authors: Corigliano MG,Fenoy I,Sander V,Maglioco A,Goldman A,Clemente M

    更新日期:2013-12-02 00:00:00

  • Safety and immunogenicity of measles-mumps-rubella vaccine in children with congenital immunodeficiency (DiGeorge syndrome).

    abstract::Live attenuated vaccines are usually contraindicated in patients with congenital or acquired immunodeficiency. On the other hand, infections due to wild type virus may be particularly severe in patients with low levels of T cells. The aim of the present study was to evaluate safety and immunogenicity of measles-mumps-...


    pub_type: 杂志文章


    authors: Azzari C,Gambineri E,Resti M,Moriondo M,Betti L,Saldias LR,G Gelli AM,Vierucci A

    更新日期:2005-02-25 00:00:00

  • Economics of polio vaccination in the post-eradication era: should OPV-using countries adopt IPV?

    abstract::The continued use of oral polio vaccine (OPV) poses a threat to polio virus eradication. Stopping all polio vaccination in the post-certification era is no longer considered to be a practical option. Policy makers agree that OPV use must stop immediately after certification. Therefore, the pragmatic alternative is for...


    pub_type: 杂志文章


    authors: Khan MM

    更新日期:2008-04-07 00:00:00

  • Immune responses and protective efficacy of a recombinant swinepox virus expressing HA1 against swine H1N1 influenza virus in mice and pigs.

    abstract::Swine influenza virus (SIV) is not only an important respiratory pathogen in pigs but also a potent threat to human health. Although immunization with recombinant poxviruses expressing protective antigens as vaccines has been widely used for against many infectious diseases, development of recombinant swinepox virus (...


    pub_type: 杂志文章


    authors: Xu J,Huang D,Liu S,Lin H,Zhu H,Liu B,Lu C

    更新日期:2012-04-26 00:00:00

  • Safety and immunogenicity of fully liquid and lyophilized formulations of an investigational trivalent group B streptococcus vaccine in healthy non-pregnant women: Results from a randomized comparative phase II trial.

    abstract:BACKGROUND:We evaluated the safety and immunogenicity of liquid and lyophilized formulations of an investigational trivalent group B streptococcus (GBS) vaccine in non-pregnant women and assessed the formulations' equivalence in terms of serotype-specific immune response. METHODS:This phase II, randomized, comparative...


    pub_type: 杂志文章


    authors: Beran J,Leroux-Roels G,Van Damme P,de Hoon J,Vandermeulen C,Al-Ibrahim M,Johnson C,Peterson J,Baker S,Seidl C,Dreisbach A,Karsten A,Corsaro B,Henry O,Lattanzi M,Bebia Z

    更新日期:2020-04-03 00:00:00

  • Burkholderia pseudomallei and Burkholderia mallei vaccines: Are we close to clinical trials?

    abstract::B. pseudomallei is the cause of melioidosis, a serious an often fatal disease of humans and animals. The closely related bacterium B. mallei, which cases glanders, is considered to be a clonal derivative of B. pseudomallei. Both B. pseudomallei and B. mallei were evaluated by the United States and the former USSR as p...


    pub_type: 杂志文章,评审


    authors: Titball RW,Burtnick MN,Bancroft GJ,Brett P

    更新日期:2017-10-20 00:00:00

  • Response to conjugate pneumococcal and Haemophilus influenzae type b vaccines in asplenic patients.

    abstract::We determined the immunogenicity of conjugated Haemophilus influenzae type b and pneumococcal vaccines by quantitative analysis of the antibody response in asplenic patients. To that end, we vaccinated 92 patients with a conjugated Hib vaccine and 54 received two doses of conjugated pneumococcal vaccine (PCV7), follow...


    pub_type: 杂志文章


    authors: Meerveld-Eggink A,de Weerdt O,van Velzen-Blad H,Biesma DH,Rijkers GT

    更新日期:2011-01-17 00:00:00

  • Association of both consistency and strength of self-reported clinician recommendation for HPV vaccination and HPV vaccine uptake among 11- to 12-year-old children.

    abstract:PURPOSE:We tested the hypotheses that consistency and strength of clinician recommendation of the human papillomavirus (HPV) vaccination would be associated with vaccine delivery rates. METHODS:From October 2015 through January 2016, we conducted a survey of primary care clinicians (n=227) in Southeastern Minnesota to...


    pub_type: 杂志文章


    authors: Finney Rutten LJ,St Sauver JL,Beebe TJ,Wilson PM,Jacobson DJ,Fan C,Breitkopf CR,Vadaparampil ST,MacLaughlin KL,Jacobson RM

    更新日期:2017-10-27 00:00:00

  • A pilot study on intradermal vaccination of Japanese rabies vaccine for pre-exposure immunization.

    abstract::Japanese rabies vaccine, a purified chick embryo cell vaccine manufactured by Kaketsuken (PCEC-K), is normally given subcutaneously; however, this requires a large amount of vaccine, and the pre-exposure vaccination regimen requires 6 months to complete. These factors often hamper appropriate vaccination. Therefore, w...


    pub_type: 杂志文章


    authors: Shiota S,Khawplod P,Ahmed K,Mifune K,Nishizono A

    更新日期:2008-11-25 00:00:00

  • BCG vaccine may generate cross-reactive T cells against SARS-CoV-2: In silico analyses and a hypothesis.

    abstract::The world is facing the rising emergency of SARS-CoV-2. The outbreak of COVID-19 has caused a global public health and economic crisis.Recent epidemiological studies have shown that a possible association of BCG vaccination program with decreased COVID-19-related risks, suggesting that BCG may provide protection again...


    pub_type: 杂志文章


    authors: Tomita Y,Sato R,Ikeda T,Sakagami T

    更新日期:2020-09-22 00:00:00

  • A ML29 reassortant virus protects guinea pigs against a distantly related Nigerian strain of Lassa virus and can provide sterilizing immunity.

    abstract::Lassa virus (LASV) is responsible for the deaths of thousands of people in West Africa annually. Genetic diversity among LASV strains is the highest among the Arenaviridae and represents a great challenge for vaccine development. Guinea pigs vaccinated with a ML29 reassortant vaccine experienced sterilizing immunity a...


    pub_type: 杂志文章


    authors: Carrion R Jr,Patterson JL,Johnson C,Gonzales M,Moreira CR,Ticer A,Brasky K,Hubbard GB,Moshkoff D,Zapata J,Salvato MS,Lukashevich IS

    更新日期:2007-05-16 00:00:00

  • Expression kinetics of the interleukin-2/immunoglobulin (IL-2/Ig) plasmid cytokine adjuvant.

    abstract::The development of strategies to augment the immunogenicity of plasmid DNA vaccines is critical for improving their clinical utility. One such strategy involves the coadministration of plasmid cytokine adjuvants with DNA vaccines. Although a large number of plasmid cytokines have shown promise as adjuvants in preclini...


    pub_type: 杂志文章


    authors: Barouch DH,Truitt DM,Letvin NL

    更新日期:2004-08-13 00:00:00

  • Highly efficient production of a dengue pseudoinfectious virus.

    abstract::Dengue is a major infectious disease that affects people living in tropical and subtropical regions around the world. The causative agents are dengue virus serotype 1, 2, 3, and 4 (DENV1, 2, 3, and 4). Developing a vaccine for dengue is a high priority for public health, but traditional methods have faced numerous obs...


    pub_type: 杂志文章


    authors: Pang X,Guo Y,Zhou Y,Fu W,Gu X

    更新日期:2014-06-24 00:00:00

  • Study of integrated protective immunity induced in rhesus macaques by the intradermal administration of a bivalent EV71-CA16 inactivated vaccine.

    abstract::Enterovirus type 71 (EV71) and coxsackievirus A 16 (CA16) are recognized as the major pathogens responsible for human hand-foot-mouth disease. To develop a bivalent EV71-CA16 vaccine, rhesus macaques immunized with two doses of this vaccine via the intradermal route were challenged with EV71 or CA16, and their clinica...


    pub_type: 杂志文章


    authors: Fan S,Liao Y,Jiang G,Jiang L,Wang L,Xu X,Feng M,Yang E,Zhang Y,Cui W,Li Q

    更新日期:2020-02-18 00:00:00

  • IMP321 (sLAG-3) safety and T cell response potentiation using an influenza vaccine as a model antigen: a single-blind phase I study.

    abstract::sLAG-3 (IMP321), a natural high affinity ligand for MHC class II, was tested for safety, tolerability and its ability to increase Th-1-type T cell responses to a commercial trivalent split influenza vaccine (Agrippal) in a phase I single-blinded, randomized, controlled clinical trial. Twenty healthy volunteers were fi...


    pub_type: 杂志文章,随机对照试验


    authors: Brignone C,Grygar C,Marcu M,Perrin G,Triebel F

    更新日期:2007-06-11 00:00:00

  • Altered proportions of circulating CXCR5+ helper T cells do not dampen influenza vaccine responses in children with rheumatic disease.

    abstract::Biological therapy options for the treatment of rheumatic disease target molecules that can affect the cross-talk between innate and adaptive immune responses upon vaccination. Influenza vaccination in children with rheumatic disease has been recommended, but there are only sparse data on the quality of vaccine respon...


    pub_type: 杂志文章


    authors: Laestadius Å,Ingelman-Sundberg HM,Myrberg IH,Verme A,Sundberg E,Schweiger B,Saghafian-Hedengren S,Nilsson A

    更新日期:2019-06-19 00:00:00

  • Recombinant herpesvirus of turkeys as a vector-based vaccine against highly pathogenic H7N1 avian influenza and Marek's disease.

    abstract::A major challenge for poultry vaccination is the design of vaccines that protect against multiple pathogens via a single protective dose delivered through mass vaccination methods. In this investigation, we examined herpesvirus of turkeys (HVT) as a vaccine vector for delivery of haemagglutinin (HA) antigen of highly ...


    pub_type: 杂志文章


    authors: Li Y,Reddy K,Reid SM,Cox WJ,Brown IH,Britton P,Nair V,Iqbal M

    更新日期:2011-10-26 00:00:00

  • Influence of adjuvant-active peptidoglycan monomer on specific T cell responses in mice.

    abstract::Peptidoglycan monomer (PGM) originating from Brevibacterium divaricatum is a non-toxic, non-pyrogenic, water-soluble immunostimulator. It potentiates humoral immune response to ovalbumin (OVA) in mice upregulating both immunoglobulin (IgG) 1 and IgG2a antibody subclasses. This study concerns the influence of PGM on T ...


    pub_type: 杂志文章


    authors: Halassy Spoljar B,Cimbora T,Hanzl-Dujmović I,Dojnović B,Sabioncello A,Krstanović M,Tomasić J

    更新日期:2002-10-04 00:00:00

  • Collecting and using reliable vaccination coverage survey estimates: Summary and recommendations from the "Meeting to share lessons learnt from the roll-out of the updated WHO Vaccination Coverage Cluster Survey Reference Manual and to set an operational

    abstract::Household surveys are frequently used as means of vaccination coverage measurement, but obtaining accurate survey estimates present several challenges. In 2015, the World Health Organization (WHO) released a working draft of its updated Vaccination Coverage Survey Reference Manual that moved well beyond the traditiona...




    authors: Danovaro-Holliday MC,Dansereau E,Rhoda DA,Brown DW,Cutts FT,Gacic-Dobo M

    更新日期:2018-08-16 00:00:00

  • Age-dependent immune responses and immune protection after avian coronavirus vaccination.

    abstract::Infectious bronchitis virus (IBV) is an endemic disease of chickens and a major contributor to economic losses for the poultry industry despite vaccination. Recent observations indicated that chicks may have an immature immune system immediately after hatching when vaccinated for IBV. Therefore we hypothesized that ea...


    pub_type: 杂志文章


    authors: van Ginkel FW,Padgett J,Martinez-Romero G,Miller MS,Joiner KS,Gulley SL

    更新日期:2015-05-28 00:00:00

  • Cost-effectiveness of mass dog rabies vaccination strategies to reduce human health burden in Flores Island, Indonesia.

    abstract::The cost-effectiveness of different mass dog rabies vaccination strategies, defined as the costs per year of life lost (YLL) averted was evaluated for a period of 10 years by means of a dynamic simulation study for a typical village on Flores Island. In the base strategy (no dog vaccination and no post-exposure treatm...


    pub_type: 杂志文章


    authors: Wera E,Mourits MCM,Hogeveen H

    更新日期:2017-12-04 00:00:00

  • Uptake of pandemic influenza (H1N1)-2009 vaccines in Brazil, 2010.

    abstract::In 2010, the Brazilian Ministry of Health organized a mass vaccination campaign of selected priority groups in response to the 2009 H1N1 influenza pandemic. The campaign was conducted in six phases from March to July, 2010. Priority groups included healthcare professionals, indigenous persons, pregnant women, young ch...


    pub_type: 杂志文章,评审


    authors: Domingues CM,de Oliveira WK,Brazilian Pandemic Influenza Vaccination Evaluation Team.

    更新日期:2012-07-06 00:00:00

  • Expression of pertussis toxin subunit S4 as an intracytoplasmic protein in Bacillus subtilis.

    abstract::The expression and secretion of pertussis toxin subunits S1 to S5 in Bacillus subtilis by the aid of a bacillary signal sequence has been reported. While secretion of subunit S1 was high, that of others was low. Ways have now been explored to improve the yield, using S4 as an example. The addition of a protease inhibi...


    pub_type: 杂志文章


    authors: Himanen JP,Taira S,Sarvas M,Saris P,Runeberg-Nyman K

    更新日期:1990-12-01 00:00:00

  • Epitope polarity and adjuvants influence the fine specificity of the humoral response against Semliki Forest virus specific peptide vaccines.

    abstract::The humoral response to synthetic peptide vaccines against Semliki Forest virus (SFV) in H-2d BALB/c mice was investigated with the enzyme linked immunosorbent assay and the pepscan technique. The peptide vaccines consisted of amino acid sequences 240-255 (B) and 137-151 (T) of the E2 membrane protein of SFV colinearl...


    pub_type: 杂志文章


    authors: Fernández IM,Harmsen M,Benaissa-Trouw BJ,Stuij I,Puyk W,Meloen RH,Snippe H,Kraaijeveld CA

    更新日期:1998-10-01 00:00:00

  • Persistence of protection through 33 months of age provided by immunization in infancy with two three-component acellular pertussis vaccines. Stage II Working Group.

    abstract::A large, randomized, placebo-controlled clinical trial in Italy on two three-component pertussis vaccines, given as DTaP in infancy, one manufactured by SmithKline and Beecham (SB) and one by Chiron Biocine (CB), found each vaccine to be 84% efficacious through the average age of 24 months. The cohort of children enro...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Salmaso S,Mastrantonio P,Wassilak SG,Giuliano M,Anemona A,Giammanco A,Tozzi AE,Ciofi degli Atti ML,Greco D

    更新日期:1998-08-01 00:00:00