Salmonella Enteritidis with double deletion in phoPfliC--a potential live Salmonella vaccine candidate with novel characteristics for use in chickens.


:Salmonella Enteritidis mutants with deletions in phoP, fliC or phoPfliC were tested for their virulence and their ability to induce parameters of the innate and adaptive immunity in addition to their potential for serological differentiation between vaccinated, non-vaccinated and infected chickens. The double phoPfliC deletion mutant was sufficiently attenuated but not diminished in its capability to inhibit the caecal colonisation and systemic invasion of homologous Salmonella Enteritidis shortly after administration of the vaccine strain to very young chicks. Immunisation with the attenuated ΔphoPfliC mutant resulted in protective effects which were only slightly and insignificantly lower than after "immunisation" with a Salmonella wild-type strain, indicating the capability to induce an intense adaptive immune response and protection against Salmonella exposure in older chickens. The deletion in fliC enabled the effective the differentiation between immunised and infected chickens using a commercially available ELISA kit. The double phoPfliC deletion mutant of Salmonella Enteritidis might be a potential and promising live Salmonella vaccine candidate with novel characteristics for use in poultry.






Methner U,Barrow PA,Berndt A,Rychlik I




Has Abstract


2011-04-12 00:00:00
















  • Tick-borne encephalitis--pathogenesis, clinical course and long-term follow-up.

    abstract::The prospective studies available today confirm the experience gained from several retrospective studies that TBE is a disease with a severe acute clinical course and considerable long-term morbidity. A defined post-encephalitic TBE syndrome exists, causing long-lasting morbidity that often affects the quality of life...


    pub_type: 杂志文章,评审


    authors: Haglund M,Günther G

    更新日期:2003-04-01 00:00:00

  • Attitudes to HPV vaccination among mothers in the British Jewish community: reasons for accepting or declining the vaccine.

    abstract:OBJECTIVE:This study aimed to explore attitudes to human papillomavirus (HPV) vaccination and reasons for accepting or declining the vaccine in the British Jewish community. METHODS:A qualitative approach was used to explore maternal attitudes towards HPV vaccination. Participants were mothers of girls who had been of...


    pub_type: 杂志文章


    authors: Gordon D,Waller J,Marlow LA

    更新日期:2011-10-06 00:00:00

  • Comparison of adjuvants to optimize influenza neutralizing antibody responses.

    abstract::Seasonal influenza vaccines represent a positive intervention to limit the spread of the virus and protect public health. Yet continual influenza evolution and its ability to evade immunity pose a constant threat. For these reasons, vaccines with improved potency and breadth of protection remain an important need. We ...


    pub_type: 杂志文章


    authors: Rudicell RS,Garinot M,Kanekiyo M,Kamp HD,Swanson K,Chou TH,Dai S,Bedel O,Simard D,Gillespie RA,Yang K,Reardon M,Avila LZ,Besev M,Dhal PK,Dharanipragada R,Zheng L,Duan X,Dinapoli J,Vogel TU,Kleanthous H,Mascola J

    更新日期:2019-09-30 00:00:00

  • Development of a candidate reference material for adventitious virus detection in vaccine and biologicals manufacturing by deep sequencing.

    abstract:BACKGROUND:Unbiased deep sequencing offers the potential for improved adventitious virus screening in vaccines and biotherapeutics. Successful implementation of such assays will require appropriate control materials to confirm assay performance and sensitivity. METHODS:A common reference material containing 25 target ...


    pub_type: 杂志文章


    authors: Mee ET,Preston MD,Minor PD,Schepelmann S,CS533 Study Participants.

    更新日期:2016-04-12 00:00:00

  • Interferon-gamma-inducing oral vaccination with Leishmania amazonensis antigens protects BALB/c and C57BL/6 mice against cutaneous leishmaniasis.

    abstract::The induction of oral tolerance against disease-inducing antigens has emerged as a feasible strategy to prevent immunopathologies. In this study, we investigated the effect of oral immunization with whole antigens of Leishmania amazonensis promastigotes (LaAg) on murine cutaneous leishmaniasis. We found that two oral ...


    pub_type: 杂志文章


    authors: Pinto EF,de Mello Cortezia M,Rossi-Bergmann B

    更新日期:2003-09-08 00:00:00

  • Detecting AIDS restriction genes: from candidate genes to genome-wide association discovery.

    abstract::The screening of common genetic polymorphisms among candidate genes for AIDS pathology in HIV exposed cohort populations has led to the description of 20 AIDS restriction genes (ARGs), variants that affect susceptibility to HIV infection or to AIDS progression. The combination of high-throughput genotyping platforms a...


    pub_type: 杂志文章


    authors: Hutcheson HB,Lautenberger JA,Nelson GW,Pontius JU,Kessing BD,Winkler CA,Smith MW,Johnson R,Stephens R,Phair J,Goedert JJ,Donfield S,O'Brien SJ

    更新日期:2008-06-06 00:00:00

  • An approach for preparing and responding to adverse events following immunization reported after hepatitis B vaccine birth dose administration.

    abstract::The success of immunization programs in lowering the incidence of vaccine preventable diseases (VPDs) has led to increased public attention on potential health risks associated with vaccines. As a result, a scientifically rigorous response to investigating reported adverse events following immunization (AEFI) and effe...


    pub_type: 杂志文章,评审


    authors: Gidudu JF,Shaum A,Habersaat K,Wilhelm E,Woodring J,Mast E,Zuber P,Amarasinghe A,Nelson N,Kabore H,Abad N,Tohme RA

    更新日期:2020-11-17 00:00:00

  • Archaeal glycolipid adjuvanted vaccines induce strong influenza-specific immune responses through direct immunization in young and aged mice or through passive maternal immunization.

    abstract::Vaccine induced responses are often weaker in those individuals most susceptible to infection, namely the very young and the elderly, highlighting the need for safe and effective vaccine adjuvants. Herein we evaluated different archaeosome formulations as an adjuvant to the H1N1 influenza hemagglutinin protein and com...


    pub_type: 杂志文章


    authors: Stark FC,Akache B,Ponce A,Dudani R,Deschatelets L,Jia Y,Sauvageau J,Williams D,Jamshidi MP,Agbayani G,Wachholz K,Harrison BA,Li X,Krishnan L,Chen W,McCluskie MJ

    更新日期:2019-11-08 00:00:00

  • Four year immunogenicity of the RTS,S/AS02(A) malaria vaccine in Mozambican children during a phase IIb trial.

    abstract::Previous studies with the malaria vaccine RTS,S/AS02(A) in young children in a malaria endemic area of Mozambique have shown it to have a promising safety profile and to reduce the risk of Plasmodium falciparum infection and disease. In this study, we assessed the antibody responses to the P. falciparum and hepatitis ...


    pub_type: 杂志文章,随机对照试验


    authors: Aide P,Dobaño C,Sacarlal J,Aponte JJ,Mandomando I,Guinovart C,Bassat Q,Renom M,Puyol L,Macete E,Herreros E,Leach A,Dubois MC,Demoitie MA,Lievens M,Vekemans J,Loucq C,Ballou WR,Cohen J,Alonso PL

    更新日期:2011-08-11 00:00:00

  • Brief education to promote maternal influenza vaccine uptake: A randomized controlled trial.

    abstract:BACKGROUND:Although pregnant women are the highest priority group for seasonal influenza vaccination, maternal influenza vaccination rates remain suboptimal. The purpose of this study was to evaluate the effect of a brief education intervention on maternal influenza vaccine uptake. METHODS:During the 2013-14 and 2014-...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Wong VWY,Fong DYT,Lok KYW,Wong JYH,Sing C,Choi AY,Yuen CYS,Tarrant M

    更新日期:2016-10-17 00:00:00

  • Intranasal c-di-GMP-adjuvanted plant-derived H5 influenza vaccine induces multifunctional Th1 CD4+ cells and strong mucosal and systemic antibody responses in mice.

    abstract::Vaccination is the best available measure of limiting the impact of the next influenza pandemic. Ideally, a candidate pandemic influenza vaccine should be easy to administer and should elicit strong mucosal and systemic immune responses. Production of influenza subunit antigen in transient plant expression systems is ...


    pub_type: 杂志文章


    authors: Madhun AS,Haaheim LR,Nøstbakken JK,Ebensen T,Chichester J,Yusibov V,Guzman CA,Cox RJ

    更新日期:2011-07-12 00:00:00

  • Transepithelial immunomodulation by cholera toxin and non-toxic derivatives.

    abstract::Comparative analyses of murine dendritic cells (DC) isolated from the skin and from the intestinal mucosa after exposure to cholera toxin and its non-toxic B subunit disclose striking differences regarding the migratory and functional behaviour of these cells. The nature of the epithelial microenvironment, especially ...


    pub_type: 杂志文章


    authors: Anjuère F,Luci C,Hervouet C,Rousseau D,Czerkinsky C

    更新日期:2006-04-12 00:00:00

  • A strategy of antigen incorporation into exosomes: comparing cross-presentation levels of antigens delivered by engineered exosomes and by lentiviral virus-like particles.

    abstract::Among strategies aimed at developing new nanoparticle-based vaccines, exosomes hold much promise. They are nanovesicles released by basically all eukaryotic cell types originating from intraluminal vesicles which accumulate in multivesicular bodies. Exosomes have immunogenic properties whose strength correlates with t...


    pub_type: 杂志文章


    authors: Lattanzi L,Federico M

    更新日期:2012-11-26 00:00:00

  • ISCOMATRIX adjuvant: a potent inducer of humoral and cellular immune responses.

    abstract::ISCOMATRIX adjuvant is capable of inducing broad and potent humoral and cellular immune responses. The components are well defined and the manufacturing process is simple and robust. Many vaccines containing the ISCOMATRIX adjuvant have been tested in a range of animal models, including human and non-human primates. S...


    pub_type: 杂志文章,评审


    authors: Pearse MJ,Drane D

    更新日期:2004-06-23 00:00:00

  • The emerging role of innate immunity in protection against HIV-1 infection.

    abstract::Preventive immunization against HIV-1 infection requires a rapid immune response that does not rely exclusively on B or T cell memory. Innate immunity may fulfill this function as it may be activated directly at the time of HIV-1 transmission, inhibit early HIV-1 replication, stimulate adaptive immunity and enable spe...


    pub_type: 杂志文章,评审


    authors: Lehner T,Wang Y,Pido-Lopez J,Whittall T,Bergmeier LA,Babaahmady K

    更新日期:2008-06-06 00:00:00

  • Intranasal inoculate of influenza virus vaccine against lethal virus challenge.

    abstract::Vaccine adjuvants are essential for enhancing immune responses during vaccination. However, only a limited number of safe and effective adjuvants, especially mucosal adjuvants, are available for use in vaccines. The development of a practically applicable mucosal adjuvant is therefore urgently needed. Here, we showed ...


    pub_type: 杂志文章


    authors: Fan X,Su Q,Qiu F,Yi Y,Shen L,Jia Z,Liang P,Zou Y,Bi S

    更新日期:2018-07-05 00:00:00

  • Gene gun DNA vaccination with Rev-independent synthetic HIV-1 gp160 envelope gene using mammalian codons.

    abstract::DNA immunization with HIV envelope plasmids induce only moderate levels of specific antibodies which may in part be due to limitations in expression influenced by a species-specific and biased HIV codon usage. We compared antibody levels, Th1/Th2 type and CTL responses induced by synthetic genes encoding membrane boun...


    pub_type: 杂志文章


    authors: Vinner L,Nielsen HV,Bryder K,Corbet S,Nielsen C,Fomsgaard A

    更新日期:1999-04-23 00:00:00

  • Crossreactive neutralizing antibodies induced by immunization with caprine arthritis-encephalitis virus surface glycoprotein.

    abstract::Four Saanen goats were immunized with affinity purified gp135 surface glycoprotein (SU) of caprine arthritis-encephalitis virus isolate 79-63 (CAEV-63) and evaluated for homologous and crossreactive serum neutralizing antibodies. CAEV-63 neutralizing antibodies were detected in all goats after seven immunizations with...


    pub_type: 杂志文章


    authors: Kemp RK,Knowles DP,Perry LL,McGuire TC,Besser TE,Cheevers WP

    更新日期:2000-01-18 00:00:00

  • Evaluation of immune response to recombinant potential protective antigens of Mycoplasma hyopneumoniae delivered as cocktail DNA and/or recombinant protein vaccines in mice.

    abstract::Intramuscular immunization of mice with DNA cocktail vaccines, comprising potential protective antigens P36, P46, NrdF, and P97or P97R1 of Mycoplasma hyopneumoniae, induced strong Th1-polarized immune responses against each antigen, with only P46 eliciting a serum IgG response. Subcutaneous immunization with protein c...


    pub_type: 杂志文章


    authors: Chen AY,Fry SR,Daggard GE,Mukkur TK

    更新日期:2008-08-12 00:00:00

  • Immune response to a booster dose of enhanced potency inactivated polio vaccine administered in association with HBV vaccine in adolescents.

    abstract::The immunogenicity and reactogenicity of a booster dose of enhanced potency inactivated polio vaccine (EIPV) were evaluated in 492 healthy 12 year old adolescents. The booster was administered at the same time as the HBV vaccine compulsory in Italy at this age. Blood samples and questionnaires on reactogenicity were c...


    pub_type: 临床试验,杂志文章


    authors: Pregliasco F,Grilli G,Andreassi A,D'Addezio E,Vacca F,Squarcione S,Biasio LR,Profeta M

    更新日期:1996-03-01 00:00:00

  • The epidemiology of invasive meningococcal disease in EU/EEA countries, 2004-2014.

    abstract:BACKGROUND:Invasive meningococcal disease (IMD) is a major cause of bacterial meningitis and septicaemia although infection by some serogroups may be prevented through vaccination. We aimed to describe the epidemiology of IMD in EU/EEA countries during 2004-2014 to monitor serogroup- and age-specific trends, and compar...


    pub_type: 杂志文章


    authors: Whittaker R,Dias JG,Ramliden M,Ködmön C,Economopoulou A,Beer N,Pastore Celentano L,ECDC network members for invasive meningococcal disease.

    更新日期:2017-04-11 00:00:00

  • Trivalent inactivated influenza vaccine (IIV3) during pregnancy and six-month infant development.

    abstract:OBJECTIVE:Despite recommendations by professional organizations that all pregnant women receive inactivated influenza vaccine, safety concerns remain a barrier. Our objective was to assess the effect of trivalent influenza vaccines (IIV3) during pregnancy on parent report 6-month infant development. METHODS:We conduct...


    pub_type: 杂志文章


    authors: Avalos LA,Ferber J,Zerbo O,Naleway AL,Bulkley J,Thompson M,Cragan J,Williams J,Odouli R,Kauffman TL,Ball S,Shifflett P,Li DK

    更新日期:2020-02-28 00:00:00

  • Translating vaccine policy into action: a report from the Bill & Melinda Gates Foundation Consultation on the prevention of maternal and early infant influenza in resource-limited settings.

    abstract::Immunization of pregnant women against influenza is a promising strategy to protect the mother, fetus, and young infant from influenza-related diseases. The burden of influenza during pregnancy, the vaccine immunogenicity during this period, and the robust influenza vaccine safety database underpin recommendations tha...


    pub_type: 共识发展会议,杂志文章


    authors: Ortiz JR,Neuzil KM,Ahonkhai VI,Gellin BG,Salisbury DM,Read JS,Adegbola RA,Abramson JS

    更新日期:2012-11-26 00:00:00

  • Changes in serotype distribution of Haemophilus influenzae meningitis isolates identified through laboratory-based surveillance following routine childhood vaccination against H. influenzae type b in Brazil.

    abstract::Following routine childhood vaccination against Haemophilus influenzae type b (Hib) disease in Brazil in 1999, passive laboratory surveillance reported increasing numbers of non-b serotypes and nontypeable H. influenzae (NTHi) from meningitis cases. To characterize this increase, we analyzed data on 3910 H. influenzae...


    pub_type: 杂志文章


    authors: Zanella RC,Bokermann S,Andrade AL,Flannery B,Brandileone MC

    更新日期:2011-11-08 00:00:00

  • T-cell triggering by CD3- and CD28-binding molecules linked to a human virus-modified tumor cell vaccine.

    abstract::The aim was to develop T cell costimulatory molecules that are broadly applicable to augment anti-tumor immune responses upon application of a virus-modified tumor vaccine to cancer patients. We generated recombinant bispecific single-chain antibodies with one specificity directed against the CD3 or the CD28 antigen o...


    pub_type: 杂志文章


    authors: Haas C,Lulei M,Fournier P,Arnold A,Schirrmacher V

    更新日期:2005-03-31 00:00:00

  • Establishment of gene-vaccinated skin grafting against Toxoplasma gondii infection in mice.

    abstract::Vaccine effects of in vivo gene-vaccinated skin graft were evaluated against Toxoplasma gondii (T. gondii) infection. By using a gene gun, cDNA coding T. gondii SAG1 molecule was intracutaneously vaccinated into C57BL/6 (B6; a susceptible strain), BALB/c (a resistant strain) and (C57BL/6 x BALB/c) F1 (CBF1) mice, and ...


    pub_type: 杂志文章


    authors: Saito S,Aosai F,Rikihisa N,Mun HS,Norose K,Chen M,Kuroki T,Asano T,Ochiai T,Hata H,Ichinose M,Yano A

    更新日期:2001-02-28 00:00:00

  • How many published cases of serious adverse events after yellow fever vaccination meet Brighton Collaboration diagnostic criteria?

    abstract:OBJECTIVES:To perform a systematic review of all serious adverse events (SAEs) after yellow fever vaccination and to assess them according to Brighton Collaboration criteria. METHODS:Nine electronic databases were searched with the terms "yellow fever vaccine" and "adverse events" to 10 July 2013 (no language/date lim...


    pub_type: 杂志文章,评审


    authors: Thomas RE,Spragins W,Lorenzetti DL

    更新日期:2013-12-16 00:00:00

  • Correlates of HPV vaccination among adolescent females from Appalachia and reasons why their parents do not intend to vaccinate.

    abstract::Limited research has examined HPV vaccination in Appalachia, a region with cervical cancer disparities. We analyzed 2008-2010 National Immunization Survey-Teen data for adolescent females ages 13-17 from Appalachia (n=1951) to identify correlates of HPV vaccination and reasons why their parents do not intend to vaccin...


    pub_type: 杂志文章


    authors: Reiter PL,Katz ML,Paskett ED

    更新日期:2013-06-28 00:00:00

  • Routing dependent immune responses after experimental R848-adjuvated vaccination.

    abstract::Most traditional vaccines are administered via the intramuscular route. Other routes of administration however, can induce equal or improved protective memory responses and might provide practical advantages such as needle-free immunization, dose sparing and induction of tissue-specific (mucosal) immunity. Here we exp...


    pub_type: 杂志文章


    authors: van Aalst S,Jansen MAA,Ludwig IS,van der Zee R,van Eden W,Broere F

    更新日期:2018-03-07 00:00:00

  • A global perspective of vaccination of healthcare personnel against measles: systematic review.

    abstract::Measles transmission has been well documented in healthcare facilities. Healthcare personnel who are unvaccinated and who lack other evidence of measles immunity put themselves and their patients at risk for measles. We conducted a systematic literature review of measles vaccination policies and their implementation i...


    pub_type: 杂志文章,评审


    authors: Fiebelkorn AP,Seward JF,Orenstein WA

    更新日期:2014-08-27 00:00:00