Persistent ictal-like activity in rat entorhinal/perirhinal cortex following washout of 4-aminopyridine.


:Application of 4-aminopyridine (4-AP, 100μM) in a solution containing 0.6mM Mg(2+) and 1.2mM Ca(2+) to hippocampal-entorhinal-perirhinal slices of adult rat brain induced ictal-like epileptiform activity in entorhinal and perirhinal cortices as revealed by electrophysiological field potential recordings. The ictal-like activity persisted after washing out the 4-AP. This persistence indicated that a change had occurred in the slice so that it was now "epileptic" in the absence of the convulsant 4-AP. Induction of persistent ictal-like activity was dependent upon the concentration of divalent cations during 4-AP exposure; that is, although 4-AP caused ictal-like activity in approximately half the slices in solution containing 1.6mM Mg(2+) and 2.0mM Ca(2+), this ictal-like activity did not persist upon washout of the 4-AP. Expression of the persistent ictal-like epileptiform activity required ionotropic glutamate-mediated synaptic transmission: application of the AMPA/kainate receptor antagonist NBQX after 4-AP washout reduced persistent ictal-like activity, and the combined application of NBQX and the NMDA receptor antagonist d-AP5 completely blocked it. In order to investigate the mechanism of induction of persistent ictal-like activity, several agents were applied before the introduction of 4-AP. Application of d-AP5 did not block the onset of ictal-like activity upon introduction of 4-AP but did prevent the persistence of the ictal-like activity upon washout of the 4-AP. In contrast, induction of persistent ictal-like activity was not prevented by simultaneous application of the group I metabotropic glutamate receptor (mGluR) antagonists LY 367385 and MPEP or by application of the protein synthesis inhibitor cycloheximide. In conclusion, we have characterized a new in vitro model of epileptogenesis in which induction of ictal-like activity is dependent upon NMDA receptor activation but not upon group I mGluR activation or protein synthesis.


Epilepsy Res


Epilepsy research


Salah A,Perkins KL




Has Abstract


2011-05-01 00:00:00














  • Effects of vitamin D and paricalcitol on epileptogenesis and behavioral properties of WAG/Rij rats with absence epilepsy.

    abstract:AIM:Vitamin D (Vit D) has been considered as a neurosteroid and has a pivotal role in neuroprotection including epilepsy. Vit D regulator acts via a Vit D receptor (VDR). WAG/Rij rats have a genetically epileptic model of absence epilepsy with comorbidity of depression. The aim of the present study was to investigate t...

    journal_title:Epilepsy research

    pub_type: 杂志文章


    authors: Aygun H,Ayyildiz M,Agar E

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  • Life-threatening adverse events of antiepileptic drugs.

    abstract::Treating a patient with antiepileptic drugs (AEDs) may give rise to unexpected life-threatening adverse events. Despite extensive experimental and clinical testing to ensure safety, most AEDs on the market have been associated with cases of severe cutaneous reactions, serious hematological disorders, or hepatic failur...

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    pub_type: 杂志文章,评审


    authors: Arroyo S,de la Morena A

    更新日期:2001-11-01 00:00:00

  • Historical trend toward improved long-term outcome in childhood absence epilepsy.

    abstract::We retrospectively analyzed published studies to investigate historical trends in outcome of childhood absence epilepsy (CAE). We included patients based on onset of absence seizures in childhood, 3 Hz bilateral spike-wave discharges on EEG, and availability of seizure-free outcome data. The primary endpoint was seizu...

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    pub_type: 历史文章,杂志文章,meta分析


    authors: Morse E,Giblin K,Chung MH,Dohle C,Berg AT,Blumenfeld H

    更新日期:2019-05-01 00:00:00

  • The antidepressant drug fluoxetine inhibits persistent sodium currents and seizure-like events.

    abstract::The antidepressant drug fluoxetine (FLX) has been shown to exert antiepileptic effects in several animal models, but mixed preclinical findings and occasional reports of proconvulsant effects have led to hesitation towards its use in epileptic people. Despite being developed as a selective serotonin reuptake inhibitor...

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    更新日期:2012-08-01 00:00:00

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    abstract:OBJECTIVE:This study was undertaken to determine whether levetiracetam (Keppra) affected the pharmacokinetic or pharmacodynamic profile of digoxin in healthy adults. METHODS:Seven men and four women (19-48 years old) completed this double-blind, placebo-controlled study. Each received digoxin 0.25 mg once daily (0.5 m...

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    更新日期:2001-08-01 00:00:00

  • Scopolamine-induced convulsions in fasted animals after food intake: sensitivity of C57BL/6J mice and Sprague-Dawley rats.

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    authors: Enginar N,Nurten A,Türkmen AZ,Çağla B

    更新日期:2015-05-01 00:00:00

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    journal_title:Epilepsy research

    pub_type: 杂志文章


    authors: Hamada K,Wada JA

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  • Genetic linkage studies in familial frontal epilepsy: exclusion of the human chromosome regions homologous to the El-1 mouse locus.

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    pub_type: 杂志文章


    authors: Lopes-Cendes I,Phillips HA,Scheffer IE,Mulley JC,Desbiens R,Andermann E,Cendes F,Verret S,Andermann F,Berkovic SF

    更新日期:1995-11-01 00:00:00

  • Selective amygdalohippocampectomy versus standard temporal lobectomy in patients with mesiotemporal lobe epilepsy and unilateral hippocampal sclerosis: post-operative facial emotion recognition abilities.

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    authors: Wendling AS,Steinhoff BJ,Bodin F,Staack AM,Zentner J,Scholly J,Valenti MP,Schulze-Bonhage A,Hirsch E

    更新日期:2015-03-01 00:00:00

  • Reciprocal positive transfer between kindling of audiogenic seizures and electrical kindling of inferior colliculus.

    abstract::The behavioral and EEG concomitants of kindling produced by daily electrical stimulation of the inferior colliculus have been recorded in three series of Wistar rats: (1) non epileptic controls (NE), (2) rats susceptible to audiogenic seizures (AS), (3) acoustically susceptible rats with prior kindling of audiogenic s...

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    pub_type: 杂志文章


    authors: Hirsch E,Maton B,Vergnes M,Depaulis A,Marescaux C

    更新日期:1993-06-01 00:00:00

  • Progress report on new antiepileptic drugs: a summary of the Third Eilat Conference.

    abstract::The Third Eilat Conference on New Antiepileptic Drugs was held at the Royal Beach Hotel from May 27 to May 30, 1996. Epileptologists and scientists from 20 countries attended the conference, which was held to discuss critical issues in drug development, new antiepileptic drugs (AEDs) in development, progress reports a...

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    authors: Bialer M,Johannessen SI,Kupferberg HJ,Levy RH,Loiseau P,Perucca E

    更新日期:1996-11-01 00:00:00

  • Distinct absorption characteristics of oral formulations of valproic acid/divalproex available in the United States.

    abstract::Five oral formulations of valproic acid (VPA)/divalproex sodium are approved and commonly used in the US for treatment of epilepsy, mania/bipolar disorder and migraine prophylaxis. These formulations have unique pharmacokinetic and formulation characteristics and are designed to treat distinct patient populations. We ...

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    pub_type: 杂志文章


    authors: Dutta S,Reed RC

    更新日期:2007-03-01 00:00:00

  • Neuroactive steroids and seizure susceptibility.

    abstract::There is increasing clinical and experimental evidence that hormones, in particular sex steroid hormones, influence neuronal excitability and other brain functions. The term 'neuroactive steroids' has been coined for steroids that interact with neurotransmitter receptors. One of the best characterized actions of neuro...

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    pub_type: 杂志文章,评审


    authors: Beyenburg S,Stoffel-Wagner B,Bauer J,Watzka M,Blümcke I,Bidlingmaier F,Elger CE

    更新日期:2001-05-01 00:00:00

  • Validation of the Generalized Anxiety Disorder-7 (GAD-7) among Chinese people with epilepsy.

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    pub_type: 杂志文章


    authors: Tong X,An D,McGonigal A,Park SP,Zhou D

    更新日期:2016-02-01 00:00:00

  • Tiagabine in glial tumors.

    abstract:RATIONALE AND PURPOSE:Preliminary reports have suggested a possible 'aetiology-specific' efficacy of tiagabine (TGB) in patients with drug-resistant partial epilepsy (DRPE) related to cerebral glial tumors (GTs). This efficacy should be related to selective blocking of GAT-1 transporter by TGB. We presented our open-la...

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    更新日期:2002-03-01 00:00:00

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    authors: Gram L,Larsson OM,Johnsen AH,Schousboe A

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    pub_type: 杂志文章


    authors: Potschka H,Baltes S,Löscher W

    更新日期:2004-02-01 00:00:00

  • Clobazam shows a different antiepileptic action profile from clonazepam and zonisamide in Ihara epileptic rats.

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    journal_title:Epilepsy research

    pub_type: 杂志文章


    authors: Miura Y,Amano S,Torii R,Ihara N

    更新日期:2002-05-01 00:00:00

  • Stereotactic laser ablation of epileptogenic periventricular nodular heterotopia.

    abstract::Periventricular nodular heterotopia (PVNH) is a neuronal migrational disorder often associated with pharmacoresistant epilepsy (PRE). Resective surgery for PVNH is limited by its deep location, and the overlying eloquent cortex or white matter. Stereotactic MR guided laser interstitial thermal therapy (MRgLITT) has re...

    journal_title:Epilepsy research

    pub_type: 杂志文章


    authors: Esquenazi Y,Kalamangalam GP,Slater JD,Knowlton RC,Friedman E,Morris SA,Shetty A,Gowda A,Tandon N

    更新日期:2014-03-01 00:00:00

  • Alterations of 5-HT1A receptor-induced G-protein functional activation and relationship to memory deficits in patients with pharmacoresistant temporal lobe epilepsy.

    abstract::The 5-hydroxytryptamine-1A (5-HT1A) receptors are known to be involved in the inhibition of seizures in epilepsy. Moreover, studies propose a role for the 5-HT1A receptor in memory function; it is believed that the higher density of this receptor in the hippocampus plays an important role in its regulation. Positron e...

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    pub_type: 杂志文章


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    更新日期:2014-12-01 00:00:00

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    journal_title:Epilepsy research

    pub_type: 杂志文章


    authors: Biella G,Forti M,de Curtis M

    更新日期:1996-07-01 00:00:00

  • Grey matter anomalies in drug-naïve childhood absence epilepsy: A voxel-based morphometry study with MRI at 3.0T.

    abstract:BACKGROUND:Little is known, so far, about the cerebral structural abnormalities in drug-naïve patients with childhood absence epilepsy (CAE). We aimed to investigate regional grey matter (GM) volume differences using voxel-based morphometry (VBM) in patients and closely matched healthy control subjects. METHODS:Twenty...

    journal_title:Epilepsy research

    pub_type: 杂志文章


    authors: Wang G,Dai ZY,Song W,Wang S,Shi H,Pan P,Chen F,Xu Y,Zhong J

    更新日期:2016-08-01 00:00:00

  • Levetiracetam effect on behavioral and electrophysiological parameters in rat model of global brain ischemia.

    abstract::Post-stroke paroxysmal activity is a neurophysiological indicator of epileptogenesis and increase of seizure susceptibility, so treatments with neuroprotective activity and anti-paroxysmal activity can be more beneficial during post-ischemic period. The goal of this study was evaluation of levetiracetam (100 mg/kg, 7 ...

    journal_title:Epilepsy research

    pub_type: 杂志文章


    authors: Litvinova SA,Kutepova IS,Voronina TA,Petrunina АА

    更新日期:2020-11-01 00:00:00

  • Telomere shortening in patients with drug-resistant epilepsy.

    abstract::Epilepsy affects about 1 % of the world population. Mesial temporal lobe epilepsy (mTLE) presents with seizures initiated in hippocampus and is the most frequent form of epilepsy. About 30 % of individuals with mTLE do not respond to conventional medications maintaining seizures and consequently new lesions on a daily...

    journal_title:Epilepsy research

    pub_type: 杂志文章


    authors: Miranda DM,Rosa DV,Costa BS,Nicolau NF,Magno LAV,de Paula JJ,Romano-Silva MA

    更新日期:2020-10-01 00:00:00

  • Differential sensitivity to inverse agonists of GABA(A)/benzodiazepine receptors in rats with genetic absence-epilepsy.

    abstract::A strain of Wistar rats, genetic absence epilepsy rats from Strasbourg (GAERS), was selected and inbred over 40 generations for occurrence of spontaneous spike-wave discharges characteristic of absence seizures, simultaneously with a strain of non-epileptic rats (NER). GAERS demonstrate an excessive sensitivity to ant...

    journal_title:Epilepsy research

    pub_type: 杂志文章


    authors: Vergnes M,Boehrer A,He X,Greney H,Dontenwill M,Cook J,Marescaux C

    更新日期:2001-11-01 00:00:00

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    journal_title:Epilepsy research

    pub_type: 杂志文章


    authors: Paul E,Conant KD,Dunne IE,Pfeifer HH,Lyczkowski DA,Linshaw MA,Thiele EA

    更新日期:2010-06-01 00:00:00

  • Scalp high frequency oscillations (HFOs) in absence epilepsy: An independent component analysis (ICA) based approach.

    abstract:BACKGROUND:High frequency oscillations (HFOs) have provided a new insight in understanding ictogenesis and seizure localization. In absence seizures, HFOs were predominantly localized in the medial prefrontal cortex (MPFC) in MEG studies. METHODS:We studied HFOs (80-250 Hz) in scalp EEGs in patients with absence epile...

    journal_title:Epilepsy research

    pub_type: 杂志文章


    authors: Chaitanya G,Sinha S,Narayanan M,Satishchandra P

    更新日期:2015-09-01 00:00:00

  • The risk of asymptomatic hyperammonemia in children with idiopathic epilepsy treated with valproate: relationship to blood carnitine status.

    abstract:PURPOSE:Valproate (VPA) administration may be associated with adverse metabolic effects, among is hyperammonemia, which could suggest metabolic abnormalities as carnitine deficiency. This study aimed to evaluate the risk frequency of hyperammonemia and abnormal carnitine levels in children receiving VPA who were otherw...

    journal_title:Epilepsy research

    pub_type: 临床试验,杂志文章


    authors: Hamed SA,Abdella MM

    更新日期:2009-09-01 00:00:00

  • Increased neocortical spiking and surgical outcome after selective amygdalo-hippocampectomy.

    abstract::We studied the electrocorticogram (ECoG) before and immediately after transcortical selective amygdalo-hippocampectomy, prospectively in 13 consecutive patients and retrospectively in three others. ECoG was performed with surface and two depth electrodes inserted through T2 aimed at the amygdala and anterior hippocamp...

    journal_title:Epilepsy research

    pub_type: 杂志文章


    authors: Cendes F,Dubeau F,Olivier A,Cukiert A,Andermann E,Quesney LF,Andermann F

    更新日期:1993-12-01 00:00:00

  • Stereotactic radiofrequency amygdalohippocampectomy for the treatment of mesial temporal lobe epilepsy: correlation of MRI with clinical seizure outcome.

    abstract:SUMMARY:Stereotactic radiofrequency amygdalohippocampectomy (AHE) has been reintroduced as an alternative treatment of mesial temporal lobe epilepsy. The aim of this study was to describe MRI changes after stereotactic AHE and to correlate the hippocampal and amygdalar volumes reduction with the clinical seizure outcom...

    journal_title:Epilepsy research

    pub_type: 临床试验,杂志文章


    authors: Malikova H,Vojtech Z,Liscak R,Prochazka T,Vymazal J,Vladyka V,Keller J,Kalina M

    更新日期:2009-02-01 00:00:00