Deletion of the A35 gene from Modified Vaccinia Virus Ankara increases immunogenicity and isotype switching.


:We show here that the immunogenicity of the Modified Vaccinia Ankara MVA vaccine strain can be improved by deletion of the A35 gene, without diminishing the ability of the virus to replicate. Deletion of the A35 gene resulted in increased virus-specific immunoglobulin production, class switching to IgG isotypes, and virus-specific IFNγ-secreting splenocytes. The MVA35 deletion virus provided excellent protective efficacy against virulent virus challenge. These results suggest that A35 deletion mutant strains will have superior vaccine performance for poxvirus vaccines as well as platform vaccines for other infectious diseases and cancer.






Rehm KE,Roper RL




Has Abstract


2011-04-12 00:00:00
















  • V-1 Immunitor: oral therapeutic AIDS vaccine with prophylactic potential.

    abstract::V-1 Immunitor (V1) is a therapeutic vaccine comprising pooled HIV antigens formulated into an oral pill. Recent V1 studies demonstrated body weight gain, increase in CD4 and CD8 cells, decrease in viral load, and improved survival of end-stage AIDS patients. The potential of V1 as a prophylactic vaccine has been evalu...


    pub_type: 临床试验,杂志文章


    authors: Jirathitikal V,Sooksathan P,Metadilogkul O,Bourinbaiar AS

    更新日期:2003-01-30 00:00:00

  • The MHC-haplotype influences primary, but not memory, immune responses to an immunodominant peptide containing T- and B-cell epitopes of the caprine arthritis encephalitis virus Gag protein.

    abstract::In this report, we describe a short peptide, containing a T helper- and a B-cell epitope, located in the Gag protein of the caprine arthritis encephalitis virus (CAEV). This T-cell epitope is capable of inducing a robust T-cell proliferative response in vaccinated goats with different genetic backgrounds and to provid...


    pub_type: 杂志文章


    authors: Fluri A,Nenci C,Zahno ML,Vogt HR,Charan S,Busato A,Pancino G,Peterhans E,Obexer-Ruff G,Bertoni G

    更新日期:2006-01-30 00:00:00

  • ISCOMATRIX adjuvant: a potent inducer of humoral and cellular immune responses.

    abstract::ISCOMATRIX adjuvant is capable of inducing broad and potent humoral and cellular immune responses. The components are well defined and the manufacturing process is simple and robust. Many vaccines containing the ISCOMATRIX adjuvant have been tested in a range of animal models, including human and non-human primates. S...


    pub_type: 杂志文章,评审


    authors: Pearse MJ,Drane D

    更新日期:2004-06-23 00:00:00

  • Purification and characterization of Epstein-Barr virus gp340/220 produced by a bovine papillomavirus virus expression vector system.

    abstract::Our initial results with a bovine papilloma virus (BPV) vector expression system indicated that we could produce significant amounts of Epstein-Barr virus (EBV) gp340/220 in the supernatant of a mouse fibroblast cell line. We have now extended these findings to show that the truncated version of gp340/220, where the m...


    pub_type: 杂志文章


    authors: Madej M,Conway MJ,Morgan AJ,Sweet J,Wallace L,Qualtiere LF,Arrand JR,Mackett M

    更新日期:1992-01-01 00:00:00

  • The cost effectiveness of influenza vaccination for adults aged 50 to 64 years: a model-based analysis for Spain.

    abstract::An economic evaluation of reducing the age threshold for routine influenza vaccination in Spain from 65 to 50 years was performed. A probabilistic model was used to compare a policy based on a recommendation to vaccinate all adults aged 50-64 with the existing vaccination policy for that age group, during interpandemi...


    pub_type: 杂志文章


    authors: Aballéa S,De Juanes JR,Barbieri M,Martin M,Chancellor J,Oyagüez I,Verwee B,Largeron N

    更新日期:2007-09-28 00:00:00

  • Immunity and protection by adoptive transfer of dendritic cells transfected with hepatitis C NS3/4A mRNA.

    abstract::In this study, we tested the hypothesis that adoptive transfer of dendritic cells (DCs) transfected ex vivo with mRNA encoding hepatitis C virus (HCV) NS3/4A would initiate potent HCV-specific protective immune responses in vivo. Murine DCs were transfected with NS3/4A mRNA or eGFP mRNA using either electroporation or...


    pub_type: 杂志文章


    authors: Yu H,Babiuk LA,van Drunen Littel-van den Hurk S

    更新日期:2007-02-26 00:00:00

  • Immunogenicity of candidate hepatitis E virus DNA vaccine expressing complete and truncated ORF2 in mice.

    abstract::Hepatitis E virus (HEV) is a major cause of enterically transmitted acute hepatitis of adults in developing nations. Our present studies show that, the complete ORF2 gene (1-660 amino acids, a.a.) coding for capsid protein of HEV as candidate DNA vaccine induced significant specific humoral and cellular immune respons...


    pub_type: 杂志文章


    authors: Deshmukh TM,Lole KS,Tripathy AS,Arankalle VA

    更新日期:2007-05-30 00:00:00

  • Antibody persistence and booster response in adolescents and young adults 4 and 7.5 years after immunization with 4CMenB vaccine.

    abstract:BACKGROUND:Data on duration of protection against invasive meningococcal disease post-vaccination with the recombinant, 4-component, meningococcal serogroup B vaccine (4CMenB) are limited. We evaluated bactericidal activity persistence in adolescents/young adults up to 7.5 years post-primary vaccination with 4CMenB, an...


    pub_type: 杂志文章,多中心研究


    authors: Nolan T,Santolaya ME,de Looze F,Marshall H,Richmond P,Henein S,Rheault P,Heaton K,Perrett KP,Garfield H,Gupta A,Ferguson M,D'Agostino D,Toneatto D,O'Ryan M

    更新日期:2019-02-21 00:00:00

  • Adjuvant activity of peptidoglycan monomer and its metabolic products.

    abstract::Peptidoglycan monomer (PGM) is a natural compound of bacterial origin. It is a non-toxic, non-pyrogenic, water-soluble immunostimulator potentiating humoral immune response to ovalbumin (OVA) in mice. It is fast degraded and its metabolic products-the pentapeptide (PP) and the disaccharide (DS)-are excreted from the m...


    pub_type: 杂志文章


    authors: Halassy B,Krstanović M,Frkanec R,Tomasić J

    更新日期:2003-02-14 00:00:00

  • Enhanced immune response by amphotericin B following NS1 protein prime-oral recombinant Salmonella vaccine boost vaccination protects mice from dengue virus challenge.

    abstract::A recombinant vaccine strain SL3261/pLT105 of attenuated aroA Salmonella enterica serovar Typhimurium SL3261 strain expressing a secreted dengue virus type 2 non-structural NS1 and Yersinia pestis F1 (Caf1) fusion protein, rNS1:Caf1, was generated. Immunological evaluation was performed by prime-boost vaccine regimen....


    pub_type: 杂志文章


    authors: Liu WT,Lin WT,Tsai CC,Chuang CC,Liao CL,Lin HC,Hung YW,Huang SS,Liang CC,Hsu HL,Wang HJ,Liu YT

    更新日期:2006-07-26 00:00:00

  • A Japanese encephalitis virus vaccine candidate strain is attenuated by decreasing its interferon antagonistic ability.

    abstract::Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes acute encephalitis with high mortality in humans. To understand the virus-host interactions that influence JEV virulence, we determined the lethality of a neurovirulent (RP-9) and an attenuated (RP-2ms) variant of JEV in several immunodeficie...


    pub_type: 杂志文章


    authors: Liang JJ,Liao CL,Liao JT,Lee YL,Lin YL

    更新日期:2009-05-11 00:00:00

  • How many published cases of serious adverse events after yellow fever vaccination meet Brighton Collaboration diagnostic criteria?

    abstract:OBJECTIVES:To perform a systematic review of all serious adverse events (SAEs) after yellow fever vaccination and to assess them according to Brighton Collaboration criteria. METHODS:Nine electronic databases were searched with the terms "yellow fever vaccine" and "adverse events" to 10 July 2013 (no language/date lim...


    pub_type: 杂志文章,评审


    authors: Thomas RE,Spragins W,Lorenzetti DL

    更新日期:2013-12-16 00:00:00

  • Tick-borne encephalitis--pathogenesis, clinical course and long-term follow-up.

    abstract::The prospective studies available today confirm the experience gained from several retrospective studies that TBE is a disease with a severe acute clinical course and considerable long-term morbidity. A defined post-encephalitic TBE syndrome exists, causing long-lasting morbidity that often affects the quality of life...


    pub_type: 杂志文章,评审


    authors: Haglund M,Günther G

    更新日期:2003-04-01 00:00:00

  • Post-exposure rabies prophylaxis: establishing effectiveness of abbreviated treatments.

    abstract::Several publications have reported recently the apparently successful use of human diploid cell rabies vaccine (HDCV) in volumes of less than the 6 X 1.0 cm3 dosage schedule currently recommended by the World Health Organization for post-exposure rabies prophylaxis. The newer proposed treatments are advocated to econo...


    pub_type: 杂志文章


    authors: Gross EM,Bearman JE

    更新日期:1985-09-01 00:00:00

  • Combination vaccines in the South African setting.

    abstract::The number of vaccines available and included as part of the national immunization schedules, has increased significantly over the past few decades. This impacts on patient/parent compliance and creates a challenge for health care providers for implementation of schedules necessitating training and infrastructure impr...


    pub_type: 杂志文章,评审


    authors: Visser A,Hoosen A

    更新日期:2012-09-07 00:00:00

  • The tuberculosis vaccine H4:IC31 is safe and induces a persistent polyfunctional CD4 T cell response in South African adults: A randomized controlled trial.

    abstract:BACKGROUND:New, more effective vaccines to prevent tuberculosis (TB) disease are needed urgently. H4:IC31 is an investigational vaccine that contains a fusion protein of the immunodominant antigens TB10.4 and Ag85B, formulated in IC31 adjuvant. We assessed the safety and immunogenicity of H4:IC31 in South African adult...


    pub_type: 杂志文章,随机对照试验


    authors: Geldenhuys H,Mearns H,Miles DJ,Tameris M,Hokey D,Shi Z,Bennett S,Andersen P,Kromann I,Hoff ST,Hanekom WA,Mahomed H,Hatherill M,Scriba TJ,H4:IC31 Trial Study Group.,van Rooyen M,Bruce McClain J,Ryall R,de Bruyn G,H4:

    更新日期:2015-07-09 00:00:00

  • A recombinant fowl adenovirus expressing the S1 gene of infectious bronchitis virus protects against challenge with infectious bronchitis virus.

    abstract::The spike peplomer S1 subunit sequence from avian infectious bronchitis virus (IBV) Vic S strain was expressed in a plasmid under the control of the fowl adenovirus (FAV) major late promoter (MLP). Two recombinants were constructed in FAV serotype 8 (FAV 8) by inserting the expression cassette between the SnaBI and Xb...


    pub_type: 杂志文章


    authors: Johnson MA,Pooley C,Ignjatovic J,Tyack SG

    更新日期:2003-06-20 00:00:00

  • Comparison of the safety and immunogenicity of concomitant and sequential administration of an adult formulation tetanus and diphtheria toxoids adsorbed combined with acellular pertussis (Tdap) vaccine and trivalent inactivated influenza vaccine in adults

    abstract::The annual contact for influenza vaccination provides an opportunity to ensure that adults have received other recommended vaccines such as Tdap. Healthy 19-64 year-olds were randomized to receive concomitant administration of Tdap and influenza vaccines or influenza vaccine followed (in 4-6 weeks by) Tdap. 720 partic...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: McNeil SA,Noya F,Dionne M,Predy G,Meekison W,Ojah C,Ferro S,Mills EL,Langley JM,Halperin SA

    更新日期:2007-04-30 00:00:00

  • Aerosol vaccination induces robust protective immunity to homologous and heterologous influenza infection in mice.

    abstract::Live-attenuated influenza vaccine (LAIV) delivered by large droplet intranasal spray is efficacious against infection. However, many of the large droplets are trapped in the external nares and do not reach the target nasal airway tissues. Smaller droplets might provide better distribution yielding similar protection w...


    pub_type: 杂志文章


    authors: Smith JH,Brooks P,Johnson S,Tompkins SM,Custer KM,Haas DL,Mair R,Papania M,Tripp RA

    更新日期:2011-03-21 00:00:00

  • Global distribution of group A rotavirus strains in horses: a systematic review.

    abstract::Group A rotavirus (RVA) is a major cause of diarrhea and diarrhea-related mortality in foals in parts of the world. In addition to careful horse farm management, vaccination is the only known alternative to reduce the RVA associated disease burden on horse farms. The precise evaluation of vaccine effectiveness against...


    pub_type: 杂志文章,评审


    authors: Papp H,Matthijnssens J,Martella V,Ciarlet M,Bányai K

    更新日期:2013-11-19 00:00:00

  • The adjuvant effect of synthetic oligodeoxynucleotide containing CpG motif converts the anti-Haemophilus influenzae type b glycoconjugates into efficient anti-polysaccharide and anti-carrier polyvalent vaccines.

    abstract::Synthetic oligodeoxynucleotides containing CpG immunostimulatory sequences (ISS) have been shown to act as potent adjuvants of type 1 immune responses when co-administered with protein or peptide vaccines. We have recently shown that ISS can increase the anti-polysaccharide (CHO) and anti-tetanus toxoid (TT) or anti-d...


    pub_type: 杂志文章


    authors: von Hunolstein C,Mariotti S,Teloni R,Alfarone G,Romagnoli G,Orefici G,Nisini R

    更新日期:2001-04-30 00:00:00

  • Virus-like particles exhibit potential as a pan-filovirus vaccine for both Ebola and Marburg viral infections.

    abstract::A safe and effective pan-filovirus vaccine is highly desirable since the filoviruses Ebola virus (EBOV) and Marburg virus (MARV) cause highly lethal disease typified by unimpeded viral replication and severe hemorrhagic fever. Previously, we showed that expression of the homologous glycoprotein (GP) and matrix protein...


    pub_type: 杂志文章


    authors: Swenson DL,Warfield KL,Negley DL,Schmaljohn A,Aman MJ,Bavari S

    更新日期:2005-04-27 00:00:00

  • Nef modulates the immunogenicity of Gag encoded in a non-infectious HIV DNA vaccine.

    abstract::Gag-CD8+ T cell responses are associated with immune control of HIV infection. Since during HIV infection Nef impairs T cell responses, we evaluated whether deletion of nef from a non-infectious HIV DNA vaccine (Delta4 Nef+), creating Delta5 Nef(-), would affect its immunogenicity. When compared with Delta4, mice inje...


    pub_type: 杂志文章


    authors: Arrode G,Hegde R,Jin Y,Singh DK,Narayan O,Chebloune Y

    更新日期:2008-07-23 00:00:00

  • Seroprevalence of diphtheria immunity among injured adults in Austria.

    abstract::Over a period of 1 year a seroepidemiological study was conducted at the outpatient clinic of a trauma department. Immunity to diphtheria was determined in serum samples from 558 injured patients (205 women and 353 men, age from 18 to 70). Diphtheria-antitoxin concentrations were measured with an enzyme immunoassay an...


    pub_type: 杂志文章


    authors: Marlovits S,Stocker R,Efstratiou A,Broughton K,Kaider A,Vécsei V,Wiedermann G,Kollaritsch H

    更新日期:2000-12-08 00:00:00

  • Mucosal immunogenicity of genetically detoxified derivatives of heat labile toxin from Escherichia coli.

    abstract::Using a fixed dose of antigen, the immune response to detoxified mutants of LT-WT following intranasal (i.n.), subcutaneous (s.c.) and oral (i.g.) immunisation has been studied. When given i.n., both LT-WT and mutant toxin, K63, generated significant levels of toxin-specific IgG in the serum, and the levels of IgA in ...


    pub_type: 杂志文章


    authors: Douce G,Giuliani MM,Giannelli V,Pizza MG,Rappuoli R,Dougan G

    更新日期:1998-07-01 00:00:00

  • A clinical trial examining the effect of increased total CRM(197) carrier protein dose on the antibody response to Haemophilus influenzae type b CRM(197) conjugate vaccine.

    abstract::CRM(197) is a carrier protein in certain conjugate vaccines. When multiple conjugate vaccines with the same carrier protein are administered simultaneously, reduced response to vaccines and/or antigens related to the carrier protein may occur. This study examined responses of infants who, in addition to diphtheria tox...


    pub_type: 杂志文章,随机对照试验


    authors: Usonis V,Bakasenas V,Lockhart S,Baker S,Gruber W,Laudat F

    更新日期:2008-08-18 00:00:00

  • Semantic network analysis of vaccine sentiment in online social media.

    abstract:OBJECTIVE:To examine current vaccine sentiment on social media by constructing and analyzing semantic networks of vaccine information from highly shared websites of Twitter users in the United States; and to assist public health communication of vaccines. BACKGROUND:Vaccine hesitancy continues to contribute to subopti...


    pub_type: 杂志文章


    authors: Kang GJ,Ewing-Nelson SR,Mackey L,Schlitt JT,Marathe A,Abbas KM,Swarup S

    更新日期:2017-06-22 00:00:00

  • A plant-produced vaccine protects mice against lethal West Nile virus infection without enhancing Zika or dengue virus infectivity.

    abstract::West Nile virus (WNV) has caused multiple global outbreaks with increased frequency of neuroinvasive disease in recent years. Despite many years of research, there are no licensed therapeutics or vaccines available for human use. One of the major impediments of vaccine development against WNV is the potential enhancem...


    pub_type: 杂志文章


    authors: Lai H,Paul AM,Sun H,He J,Yang M,Bai F,Chen Q

    更新日期:2018-03-27 00:00:00

  • Oral immunization with recombinant BCG induces cellular and humoral immune responses against the foreign antigen.

    abstract::It has been shown recently that BCG can be used as a live recombinant vaccine to stimulate immune responses. Proliferative or cytotoxic T-cell responses against several viral proteins such as HIV Gag, Env or Nef were obtained after parenteral immunization with BCG expressing these proteins. Antibody responses were als...


    pub_type: 杂志文章


    authors: Lagranderie M,Murray A,Gicquel B,Leclerc C,Gheorghiu M

    更新日期:1993-10-01 00:00:00

  • Control of tick infestations in wild roe deer (Capreolus capreolus) vaccinated with the Q38 Subolesin/Akirin chimera.

    abstract::Ticks (Acari: Ixodidae) are considered to be the most important vectors of disease-causing pathogens in domestic and wild animals, and emerging and re-emerging tick-borne diseases (TBD) exert an enormous impact on them. Wild ungulates are hosts for a wide variety of tick species and tick-borne pathogens that affect hu...


    pub_type: 杂志文章


    authors: Contreras M,San José C,Estrada-Peña A,Talavera V,Rayas E,Isabel León C,Luis Núñez J,García Fernández de Mera I,de la Fuente J

    更新日期:2020-09-22 00:00:00