Evaluation of a norovirus detection methodology for ready-to-eat foods.


:Despite recent norovirus (NoV) foodborne outbreaks related to consumption of ready-to-eat (RTE) foods, a standardized assay to detect NoV in these foods is not available yet. Therefore, the robustness of a methodology for NoV detection in RTE foods was evaluated. The NoV detection methodology consisted of direct RNA extraction with an eventual concentration step, followed by RNA purification and a multiplex RT-qPCR assay for the detection of GI and GII NoV and the murine norovirus-1 (MNV-1), the latter used as process control. The direct RNA extraction method made use of the guanidine-isothiocyanate containing reagent (Tri-reagent®, Ambion) to extract viral RNA from the food sample (basic protocol called TriShort), followed by an eventual concentration step using organic solvents (extended protocol called TriConc). To evaluate the robustness of the NoV detection method, the influence of (1) the NoV inoculum level and (2) different food types on the recovery of NoV from RTE foods was investigated. Simultaneously, the effect of two RNA purification methods (manual RNeasy minikit (Qiagen) and automated NucliSens EasyMAG (BioMérieux)) on the recovery of NoV from these foods was examined. Finally, MNV-1 was evaluated as process control. First of all, high level GI and GII NoV inocula (~10⁶ NoV genomic copies/10 g) could be recovered from penne salad samples (10 g) in at least 4 out of 6 PCRs, while low level GI and GII NoV inocula (~10⁴ NoV genomic copies/10 g) could be recovered from this food product in maximally 3 out 6 PCRs, showing a significant influence of the NoV inoculum level on its recovery. Secondly, low level GI and GII NoV inocula (10⁴ NoV genomic copies/10 g) were spiked onto 22 ready-to-eat food samples (10 g) classified in three categories (soups, deli sandwiches and composite meals). The GI and GII NoV inocula could be recovered from 20 of the 22 samples. The TriConc protocol provided better recoveries of GI and GII NoV for soups while the TriShort protocol yielded better results for the recovery of GII NoV from composite meals. NoV recovery from deli sandwiches was problematic using either protocol. Thirdly, the simultaneous comparison of two RNA purification protocols demonstrated that automated RNA purification performed equally or better compared to manual RNA extraction. Finally, MNV-1 was successfully evaluated as process control when detecting NoV in RTE foods using this detection methodology. In conclusion, the evaluated NoV detection method was capable of detecting NoV in RTE foods, although recoveries were influenced by the inoculum level and by the food type.


Int J Food Microbiol


Stals A,Baert L,De Keuckelaere A,Van Coillie E,Uyttendaele M




Has Abstract


2011-02-28 00:00:00














  • Antimicrobial resistance observed in Escherichia coli strains isolated from fecal samples of cattle and pigs in Korea during 2003-2004.

    abstract::A total of 744 Escherichia coli strains isolated from 830 fecal samples of healthy cattle and pigs in all provinces of Korea were examined for resistance to 16 antimicrobials. The most frequently observed resistance in cattle isolates was to tetracycline (30.5%), followed by resistance to streptomycin (20.4%), ampicil...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Lim SK,Lee HS,Nam HM,Cho YS,Kim JM,Song SW,Park YH,Jung SC

    更新日期:2007-05-10 00:00:00

  • Biological control of postharvest spoilage caused by Penicillium expansum and Botrytis cinerea in apple by using the bacterium Rahnella aquatilis.

    abstract::The epiphytic bacterium Rahnella aquatilis, isolated from fruit and leaves of apples, was tested for antagonistic properties against Penicillium expansum and Botrytis cinerea on Red Delicious apple fruit. In "in vitro" assays, this bacterium inhibited completely the germination of P. expansum and B. cinerea spores, bu...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Calvo J,Calvente V,de Orellano ME,Benuzzi D,Sanz de Tosetti MI

    更新日期:2007-02-15 00:00:00

  • Prediction of the adherence, growth and release of microorganisms in production chains.

    abstract::The aim of this study was to develop a mathematical model that describes the bacterial contamination of food as a result of adherence, growth and release of bacteria in process equipment. The model developed can be applied to control the bacterial quality of food products produced in process chains in which the final ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: de Jong P,te Giffel MC,Kiezebrink EA

    更新日期:2002-03-25 00:00:00

  • Occurrence of Alicyclobacillus in the fruit processing environment--a review.

    abstract::Concentrated fruit products have a significant place in modern consumption markets and are valuable semi-prepared food components to the bakery, dairy, confectionary, canning, baby food, frozen food, distilling and beverage industries. There is continuous pressure on the beverage industry to improve the quality of con...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章,评审


    authors: Steyn CE,Cameron M,Witthuhn RC

    更新日期:2011-05-14 00:00:00

  • Agricultural by-products with bioactive effects: A multivariate approach to evaluate microbial and physicochemical changes in a fresh pork sausage enriched with phenolic compounds from olive vegetation water.

    abstract::The use of phenolic compounds derived from agricultural by-products could be considered as an eco-friendly strategy for food preservation. In this study a purified phenol extract from olive vegetation water (PEOVW) was explored as a potential bioactive ingredient for meat products using Italian fresh sausage as food m...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Fasolato L,Carraro L,Facco P,Cardazzo B,Balzan S,Taticchi A,Andreani NA,Montemurro F,Martino ME,Di Lecce G,Toschi TG,Novelli E

    更新日期:2016-07-02 00:00:00

  • Improved PCR detection of Campylobacter jejuni from chicken rinses by a simple sample preparation procedure.

    abstract::Many food samples and enrichment media are inhibitory to the PCR, thereby lowering its detection capacity. A simple sample preparation method based on buoyant density centrifugation was examined for its application in PCR detection of Campylobacter jejuni from chicken rinse samples. Bacterial cells were spiked at diff...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Wang H,Farber JM,Malik N,Sanders G

    更新日期:1999-11-01 00:00:00

  • Inactivation of Bacillus cereus spores in milk by mild pressure and heat treatments.

    abstract::The objective of this work was to study the germination and subsequent inactivation of Bacillus cereus spores in milk by mild hydrostatic pressure treatment. In an introductory experiment with strain LMG6910 treated at 40 degrees C for 30 min at 0, 100, 300 and 600 MPa, germination levels were 1.5 to 3 logs higher in ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Van Opstal I,Bagamboula CF,Vanmuysen SC,Wuytack EY,Michiels CW

    更新日期:2004-04-15 00:00:00

  • Fresh-cut product sanitation and wash water disinfection: problems and solutions.

    abstract::It is well known that fresh-cut processors usually rely on wash water sanitizers to reduce microbial counts in order to maintain quality and extend shelf-life of the end product. Water is a useful tool for reducing potential contamination but it can also transfer pathogenic microorganisms. Washing with sanitizers is i...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章,评审


    authors: Gil MI,Selma MV,López-Gálvez F,Allende A

    更新日期:2009-08-31 00:00:00

  • Development of a global stochastic model relating the distribution of individual cell and population physiological states.

    abstract::Our ability to predict the lag (lambda) prior to growth of foodborne pathogens is limited by our lack of understanding of the physiological changes taking place in the individual cell during the adaptation process. Theoretical models have been developed to describe the stochastic nature of individual cells, and probab...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: McKellar RC,Lu X

    更新日期:2005-04-15 00:00:00

  • Isolation, identification and toxigenic potential of ochratoxin A-producing Aspergillus species from coffee beans grown in two regions of Thailand.

    abstract::In 2006 and 2007, 32 Thai dried coffee bean samples (Coffea arabica) from two growing sites of Chiang Mai Province, and 32 Thai dried coffee bean samples (Coffea canephora var. robusta) from two growing sites of Chumphon Province, Thailand, were collected and assessed for the distribution of fungi with the potential t...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Noonim P,Mahakarnchanakul W,Nielsen KF,Frisvad JC,Samson RA

    更新日期:2008-12-10 00:00:00

  • Microbial biodiversity, quality and shelf life of microfiltered and pasteurized extended shelf life (ESL) milk from Germany, Austria and Switzerland.

    abstract::Information on factors limiting the shelf life of extended shelf life (ESL) milk produced by microfiltration and subsequent pasteurization is very limited. In this study, three different batches of ESL milk were analyzed at different stages of the production process and during storage at 4 °C, 8 °C and 10 °C in order ...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Schmidt VS,Kaufmann V,Kulozik U,Scherer S,Wenning M

    更新日期:2012-03-01 00:00:00

  • Differential crosstalk between epithelial cells, dendritic cells and bacteria in a co-culture model.

    abstract::Intestinal epithelial cells (IECs) provide a primary physical barrier against commensal and pathogenic bacteria, but the influence of IECs in the regulation of the associated mucosal immune system remains largely unknown. The network of dendritic cells (DCs) in the vicinity of IECs is known to play a crucial role in t...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Zoumpopoulou G,Tsakalidou E,Dewulf J,Pot B,Grangette C

    更新日期:2009-04-30 00:00:00

  • Risk assessment of Salmonella in Danish meatballs produced in the catering sector.

    abstract::A modular process risk model approach was used to assess health risks associated with Salmonella spp. after consumption of the Danish meatball product (frikadeller) produced with fresh pork in a catering unit. Meatball production and consumption were described as a series of processes (modules), starting from 1.3kg me...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Møller CO,Nauta MJ,Schaffner DW,Dalgaard P,Christensen BB,Hansen TB

    更新日期:2015-03-02 00:00:00

  • Improving artificial neural networks with a pruning methodology and genetic algorithms for their application in microbial growth prediction in food.

    abstract::The application of Artificial Neural Networks (ANN) in predictive microbiology is presented in this paper. This technique was used to build up a predictive model of the joint effect of NaCl concentration, pH level and storage temperature on kinetic parameters of the growth curve of Lactobacillus plantarum using ANN an...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: García-Gimeno RM,Hervás-Martínez C,de S

    更新日期:2002-01-30 00:00:00

  • Prevalence of Clostridium botulinum types B, E and F in faecal samples from Swedish cattle.

    abstract::Faeces were collected from 60 cows at three slaughterhouses situated in southern and central Sweden. The faecal samples were collected during two sampling periods over the year, summer and winter. All samples were analysed for the presence of Clostridium botulinum spores, according to a combined selection and enrichme...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Dahlenborg M,Borch E,Rådström P

    更新日期:2003-04-25 00:00:00

  • Development of a method for the direct fermentation of semolina by selected sourdough lactic acid bacteria.

    abstract::Three obligately heterofermentative lactic acid bacteria (LAB) strains (Lactobacillus sanfranciscensis PON100336, Leuconostoc citreum PON10079 and Weissella cibaria PON10030) were used in this study as a multi-species starter culture for sourdough production. The starter inoculum was prepared and propagated in sterile...

    journal_title:International journal of food microbiology

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    authors: Alfonzo A,Urso V,Corona O,Francesca N,Amato G,Settanni L,Di Miceli G

    更新日期:2016-12-19 00:00:00

  • Origin and spoilage potential of the microbiota dominating genus Psychrobacter in sterile rehydrated salt-cured and dried salt-cured cod (Gadus morhua).

    abstract::Salt-cured and dried salt-cured cod rehydrated using sterile water and equipment have a short shelf life at 4 degrees C due to high bacterial counts. The microbiota develops off-odours which partly can be described as musty, causing sensory rejection within 7-10 days of chilled storage. The microbiota composition was ...

    journal_title:International journal of food microbiology

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    authors: Bjørkevoll I,Olsen RL,Skjerdal OT

    更新日期:2003-07-25 00:00:00

  • Effect of lauric arginate, nisin Z, and a combination against several food-related bacteria.

    abstract::The effects of lauric arginate (LAE) and nisin Z, alone or in combination, on cell damage were investigated against Escherichia coli O157:H7, Listeria monocytogenes and Brochothrix thermosphacta, by scanning electron microscopy (SEM) and transmission electron microscopy (TEM) observations, efflux of potassium and phos...

    journal_title:International journal of food microbiology

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    authors: Pattanayaiying R,H-Kittikun A,Cutter CN

    更新日期:2014-10-01 00:00:00

  • Dynamics of biofilm formation by Listeria monocytogenes on stainless steel under mono-species and mixed-culture simulated fish processing conditions and chemical disinfection challenges.

    abstract::The progressive ability of a six-strains L. monocytogenes cocktail to form biofilm on stainless steel (SS), under fish-processing simulated conditions, was investigated, together with the biocide tolerance of the developed sessile communities. To do this, the pathogenic bacteria were left to form biofilms on SS coupon...

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    authors: Papaioannou E,Giaouris ED,Berillis P,Boziaris IS

    更新日期:2018-02-21 00:00:00

  • Putrescine production by Lactococcus lactis subsp. cremoris CECT 8666 is reduced by NaCl via a decrease in bacterial growth and the repression of the genes involved in putrescine production.

    abstract::The reduction of NaCl in food is a public health priority; high NaCl intakes have been associated with serious health problems. However, it is reported that reducing the NaCl content of cheeses may lead to an increase in the content of biogenic amines (BAs). The present work examines the effect of NaCl on the accumula...

    journal_title:International journal of food microbiology

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    authors: Del Rio B,Redruello B,Ladero V,Fernandez M,Martin MC,Alvarez MA

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  • Minimal water activity for growth of Listeria monocytogenes as affected by solute and temperature.

    abstract::Two strains of Listeria monocytogenes, Scott A (serotype 4b) and Brie 1 (serotype 1b) were characterized in terms of minimum aw requirements for growth at 4 and 30 degrees C, using NaCl, glycerol and sucrose as test solutes. Both strains grew well at 30 degrees C in glycerol-supplemented tryptic soy broth (TSB), but n...

    journal_title:International journal of food microbiology

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    authors: Tapia de Daza MS,Villegas Y,Martinez A

    更新日期:1991-12-01 00:00:00

  • Occurrence of Listeria spp. in critical control points and the environment of Minas Frescal cheese processing.

    abstract::Critical control points (CCPs) associated with Minas Frescal cheese (a Brazilian soft white cheese, eaten fresh) processing in two dairy factories were determined using flow diagrams and microbiological tests for detection of Listeria monocytogenes and other species of Listeria. A total of 218 samples were collected a...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Silva IM,Almeida RC,Alves MA,Almeida PF

    更新日期:2003-03-25 00:00:00

  • Evidence that dissipation of proton motive force is a common mechanism of action for bacteriocins and other antimicrobial proteins.

    abstract::While bacteriocins from lactic acid bacteria (LAB) have generated tremendous interest among food microbiologists, they are not unique. The biosphere is awash with antimicrobial proteins such as colicins, defensins, cecropins, and magainins. These proteins share many characteristics. They are low molecular weight, cati...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章,评审


    authors: Montville TJ,Bruno ME

    更新日期:1994-12-01 00:00:00

  • Salmonella in slaughter pigs in Northern Ireland: prevalence and use of statistical modelling to investigate sample and abattoir effects.

    abstract::A cross-sectional survey of pigs at slaughter in Northern Ireland was undertaken to determine the overall prevalence of Salmonella infection. In total 513 pigs were sampled across four abattoirs, with Salmonella spp. isolated from the caecal contents of 31.4% (95% confidence interval [CI] 27.4%-35.4%) and from 40.0% (...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: McDowell SW,Porter R,Madden R,Cooper B,Neill SD

    更新日期:2007-09-15 00:00:00

  • The influence of temperature, pH, and water immersion on the high hydrostatic pressure inactivation of GI.1 and GII.4 human noroviruses.

    abstract::Detection of human norovirus (HuNoV) usually relies on molecular biology techniques, such as qRT-PCR. Since histo-blood group antigens (HBGAs) are the functional receptors for HuNoV, HuNoV can bind to porcine gastric mucin (PGM), which contains HBGA-like antigens. In this study, PGM-conjugated magnetic beads were used...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Li X,Chen H,Kingsley DH

    更新日期:2013-10-15 00:00:00

  • Costs of acute bacterial foodborne disease in Canada and the United States.

    abstract::Bacterial foodborne disease is increasing in industrialized as well as developing countries. For Canada and the United States many millions of cases are believed to occur each year, based on extrapolations of survey data, human enteric isolations and reported foodborne disease cases. The economic impact of such a larg...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章,评审


    authors: Todd EC

    更新日期:1989-12-01 00:00:00

  • Development of a predictive model for Salmonella spp. reduction in meat jerky product with temperature, potassium sorbate, pH, and water activity as controlling factors.

    abstract::The objective of this study was to develop a predictive model for the inactivation of Salmonella spp. in ground beef jerky as a function of temperature (T), pH, potassium sorbate (PS), and final water activity (aw). Following a central composite design, ground beef was combined with PS (0 to 0.3%, w/w), pH adjusted fr...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Juneja VK,Valenzuela-Melendres M,Heperkan D,Bautista D,Anderson D,Hwang CA,Peña-Ramos A,Camou JP,Torrentera-Olivera N

    更新日期:2016-11-07 00:00:00

  • DNA-based methodologies for the quantification of live and dead cells in formulated biocontrol products based on Pantoea agglomerans CPA-2.

    abstract::Pantoea agglomerans strain CPA-2 is an effective biocontrol agent (BCA) against the major postharvest pathogens present on pome and citrus fruits. Dehydration, such as freeze-drying, spray-drying and fluidized bed drying is one of the best ways to formulate BCAs. In this work, the survival of CPA-2 cells after formula...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Soto-Muñoz L,Torres R,Usall J,Viñas I,Solsona C,Teixidó N

    更新日期:2015-10-01 00:00:00

  • Control of Listeria monocytogenes in ground beef by 'Lactocin 705', a bacteriocin produced by Lactobacillus casei CRL 705).

    abstract::The effectiveness of Lactobacillus casei CRL 705 as well as that of Lactocin 705, the associated bacteriocin produced, in reducing population levels and growth of Listeria monocytogenes in sterile and non-sterile ground beef was studied. Predetermined numbers of L. monocytogenes and concentrations of Lactocin 705 were...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Vignolo G,Fadda S,de Kairuz MN,de Ruiz Holgado AA,Oliver G

    更新日期:1996-04-01 00:00:00

  • Assessing the capacity of growth, survival, and acid adaptive response of Listeria monocytogenes during storage of various cheeses and subsequent simulated gastric digestion.

    abstract::Different physicochemical and microbiological characteristics of cheeses may affect Listeria monocytogenes potential to grow, survive, or exhibit an acid adaptive response during storage and digestion. The objectives of the present study were to assess: i) the survival or growth potential of L.monocytogenes on various...

    journal_title:International journal of food microbiology

    pub_type: 杂志文章


    authors: Kapetanakou AE,Gkerekou MA,Vitzilaiou ES,Skandamis PN

    更新日期:2017-04-04 00:00:00