Abstract:
OBJECTIVE:Toll-like receptor-9 (TLR-9), a new member of the interleukin-1 receptor superfamily, was recently found to have a high level of expression in many carcinoma specimens. The objective of this study was to examine the TLR-9 expression and its role in tumour cell proliferation in oral squamous cell carcinoma. MATERIALS AND METHODS:Western blot and immunohistochemistry were used to detect TLR-9 protein in oral squamous cell carcinoma (OSCC) Tca-8113 cell lines and clinical specimens (n=60). The relationship between TLR-9 expression and clinicopathologic features was analysed. Cell proliferation and inflammatory chemokines secretion were tested by MTT and ELISA methods respectively. RESULTS:Results showed that TLR-9 expression level was higher in OSCC tissues than in paired adjacent normal tissues (P<0.01), and the expression level of TLR-9 was significantly associated with tumour size (P=0.001), tumour clinical stage (P=0.003) and Ki-67 expression (P<0.01). In vitro results also suggested that stimulation of Tca-8113 cells with TLR-9 agonist CpG-ODN could significantly increase tumour cell proliferation as well as subsequent IL-1α and IL-6 secretions (P<0.01), which could be partially inhibited by usage of anti-TLR-9 protein. CONCLUSIONS:It was therefore hypothesized that increased expression of TLR-9 may be of great value in assessing the development of OSCC, and could be used as a new target for OSCC prevention and therapy in future.
journal_name
Arch Oral Bioljournal_title
Archives of oral biologyauthors
Min R,Zun Z,Siyi L,Wenjun Y,Lizheng W,Chenping Zdoi
10.1016/j.archoralbio.2011.01.010subject
Has Abstractpub_date
2011-09-01 00:00:00pages
877-84issue
9eissn
0003-9969issn
1879-1506pii
S0003-9969(11)00030-6journal_volume
56pub_type
杂志文章abstract::These three neuropeptides were measured at daily baseline values by radioimmunoassay. Stimulated parotid saliva was collected from 31 subjects using a modified Carlson-Crittenden device affixed over Stenson's duct. Methionine enkephalin-like immunoreactivity ranged from 6.6 to 11.7 fmol/ml, with a mean of 9.3 fmol/ml....
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(92)90076-k
更新日期:1992-09-01 00:00:00
abstract:OBJECTIVE:The DNA oxidation byproduct 8-hydroxy-2'-deoxyguanosine (8-OHdG) is a well-known biomarker used to evaluate oxidative stress. We previously reported that the generation of reactive oxygen species (ROS) is increased in cultured gingival fibroblasts (GF) from patients with Down syndrome (DS). Thus, the aim of t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2013.03.017
更新日期:2013-09-01 00:00:00
abstract:OBJECTIVE:Intraperitoneal injection of a sialogogue, pilocarpine, at high concentrations induces salivation via peripheral pathways and thirst sensation via central pathways. In this study, we report that the effects of another sialagogue, cevimeline, on salivation and water intake in conscious rats differ from those o...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2006.09.002
更新日期:2007-01-01 00:00:00
abstract::The present study was designed to investigate the mechanical properties and load-relaxation of these discs in compression. Eight discs were used for the experiments. Compression was applied to specimens up to the specified strain, and a series of load-relaxation tests was conducted on each specimen from 0.25% strain t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(99)00097-7
更新日期:1999-12-01 00:00:00
abstract:OBJECTIVE:This study aimed to identify candidate genes as potential biomarkers in nasopharyngeal carcinoma (NPC) by bioinformatical analysis. METHODS:Three microarray datasets: GSE32906, GSE15170, GSE53819 were download from public database and analyzed to identify the differentially expressed genes (DEGs) between NPC...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2019.07.003
更新日期:2019-10-01 00:00:00
abstract:OBJECTIVE:The aim of the present study is to investigate the involvement of immunocompetent cells, pro-inflammatory cytokines and HSP, to evaluate a change of periodontal ligament during the initial stage of orthodontic tooth movement. DESIGN:In the present study, we investigated the distributional density of immunoco...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2010.11.010
更新日期:2011-05-01 00:00:00
abstract::The response of maxillary tissues to mucoperiosteal manipulation at different ages was studied microscopically early after operation and at maturity. Thirty-two Beagles were divided into three experimental groups, a sham-operated group and a control group. At the age of 6, 16 or 25 weeks in the experimental groups an ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(91)90033-q
更新日期:1991-01-01 00:00:00
abstract::The first indication of cartilage formation was detected on day 14.5 of pregnancy in close connection to the ossifying mandible. A separated blastema was never observed. Thus the findings support the concept that the condylar cartilage develops from already differentiated cells, not from primary undifferentiated mesen...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00105-0
更新日期:1996-01-01 00:00:00
abstract:OBJECTIVE:The purpose of this study was to clarify the interaction of vascular endothelial growth factors (VEGFs)-C and -D with cell surface foetal liver kinase-1 (Flk-1) and fms-like tyrosine kinase-4 (Flt-4) receptors in the induction and activity of osteoclasts in cultured human peripheral blood mononuclear cells (P...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2012.06.010
更新日期:2013-01-01 00:00:00
abstract::Timed-pregnant rats were injected with a total of 90 microgram of retinoic acid from day 13 until delivery. Other pregnant groups were given either a total of 390 microgram of retinol from day 5 or a total of 270 microgram of retinol from day 13 until delivery. Although the weight gains and food intake of the dams dur...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(82)90070-x
更新日期:1982-01-01 00:00:00
abstract::Small amalgam fillings were placed in maxillary and mandibular second molar and canine teeth for cine-radiographic analysis. The rosette strain gauges were bonded bilaterally to mandibular cortical bone below the second or third molars. The monkeys were placed in a restraining chair that did not restrict normal head, ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(86)90139-1
更新日期:1986-01-01 00:00:00
abstract::Tartrate-resistant acid phosphatase (TRAPase)-positive cells were examined during the early morphogenesis of mouse mandibular first molar teeth, using a simultaneous azo dye-coupling technique and confocal laser scanning microscopy (CLSM). CLSM of paraffin-embedded sections revealed that cells exhibiting TRAPase fluor...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(98)00019-3
更新日期:1998-05-01 00:00:00
abstract:OBJECTIVE:As shown in the quantitative suspension test adding lactoperoxidase to a thiocyanate (SCN(-)) hydrogen peroxide (H(2)O(2)) combination over the physiological saliva level has significant positive antimicrobial effects to a level of totally killing Streptococcus mutans, Streptococcus sanguinis, and Candida alb...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2011.04.016
更新日期:2011-12-01 00:00:00
abstract::These immunoglobulins were quantitated in 303 individuals belonging to 37 negroid and 26 caucasoid American families. Levels varied widely from individual to individual and frequency distributions were skewed toward the low levels. IgA was detected in all individuals except three children; two of whom were young and t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(88)90055-6
更新日期:1988-01-01 00:00:00
abstract::Five well-characterized foods were used to compare two systems for estimating the cariogenic potential of food: human plaque-acidity using interdental metallic touch electrodes, and cariogenicity testing in rats. Both systems identified the same food as the least cariogenic or acidogenic food and identified the same t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(85)90089-5
更新日期:1985-01-01 00:00:00
abstract::The possible role of fibronectin in dental tissue repair was investigated by comparing its distribution and that of the 165-kDa fibronectin-binding membrane protein (165 kDa-FnBP) in odontoblasts underlying carious and sound dentine. By immunoperoxidase and light microscopy, fibronectin was localized in the dentine un...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00068-z
更新日期:1995-11-01 00:00:00
abstract:OBJECTIVE:To investigate the effect of Rho kinase (ROCK) on Porphyromonas gingivalis outer membrane vesicles (OMVs)-induced suppression of endothelial nitric oxide synthase (eNOS) and explore the potential mechanism. DESIGN:Firstly, we investigated the effect of OMVs on total eNOS expression and eNOS activity in Human...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2014.12.009
更新日期:2015-03-01 00:00:00
abstract::Green tea is a leading beverage in the Far East for thousands of years; it is regarded for a long time as a health product. Green tea is important source of polyphenol antioxidants. Polyphenols including epigallocatechin 3 gallate (EGCG) constitute the most interesting components in green tea leaves. Green tea has the...
journal_title:Archives of oral biology
pub_type: 杂志文章,评审
doi:10.1016/j.archoralbio.2011.11.017
更新日期:2012-05-01 00:00:00
abstract::The recovery of fibres in the chorda tympani after repair by epineurial suture or entubulation was investigated. The combined trunk of the chorda tympani and lingual branch of the trigeminal nerve was sectioned unilaterally, repaired using either epineurial sutures or entubulation, and allowed to recover for 12 weeks....
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(94)00189-i
更新日期:1995-05-01 00:00:00
abstract:OBJECTIVE:The aim of this study is to investigate the expression of pannexin3 (Panx3) in human odontoblast-like cells (hOBs) and its hemichannel function in mediating ATP release. METHODS:RT-PCR and immunofluorescence analysis were used to detect the expression of pannexins (Panxs) in human dental pulp tissue and cult...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2015.07.005
更新日期:2015-10-01 00:00:00
abstract::The rate of synthesis of secretory proteins increases significantly in rat parotid glands after stimulated discharge of stored proteins. How any difference in the amount of secretory protein discharge affects the rate of subsequent protein synthesis, and whether this post-secretory synthesis is regulated at the level ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(89)90147-7
更新日期:1989-01-01 00:00:00
abstract::Medications commonly used in elderly people cause hyposalivation and are associated with an enhanced prevalence of dental caries. Propranolol (a beta-adrenergic antagonist) is a commonly used antihypertensive agent that is prescribed for long-term use. The purpose of this investigation was to compare the effects of th...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(93)90094-3
更新日期:1993-10-01 00:00:00
abstract:UNLABELLED:The inferior head of lateral pterygoid (IHLP) is thought to play a critical role in the generation and control of lateral jaw movements. AIM:The aim was to test the hypothesis that a change to the lateral tooth guidance (working-side occlusal alteration, OA) results in a significant change in the electromyo...
journal_title:Archives of oral biology
pub_type: 临床试验,杂志文章
doi:10.1016/j.archoralbio.2006.01.007
更新日期:2006-08-01 00:00:00
abstract::The effects of dietary calcium deficiency and subsequent replenishment of the diet with calcium alone or with xylitol were studied. Thirty 3-week-old Wistar rats were labelled with an i.p. tetracycline injection. Twenty rats were fed a diet with 0.026% calcium (Ca-deficient); 10 received a 0.5% Ca diet (controls). Aft...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(95)00036-o
更新日期:1995-08-01 00:00:00
abstract::Previous studies have reported that the abnormal development of the second deciduous molar in Down syndrome and cerebral palsy begins before birth. In view of these results we have turned our attention to the earlier stages of dental development in utero, represented by the primary canine, in order to see if we can id...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2007.04.011
更新日期:2007-10-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to analyze the characteristics of the clustered regularly interspaced short palindromic repeats (CRISPR) sites in 45 clinical Streptococcus mutans strains and their relationship to the clinical manifestations of early childhood caries (ECC). METHODS:Forty-five S. mutans strains were...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.07.023
更新日期:2017-11-01 00:00:00
abstract::The influence of indigenous Streptococcus mutans on naturally-occurring levels of IgA antibodies was studied in 42 lactating females. Breast milk, parotid and whole-saliva samples were collected and analysed by the ELISA method for IgA antibodies, reacting with antigens from Strep. mutans. All salivas and breast milk ...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/0003-9969(87)90149-x
更新日期:1987-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study is to explore patterns of non-syndromic permanent tooth agenesis in a large orthodontic patient group. DESIGN:A record review was performed in various orthodontic clinics to identify white patients with non-syndromic permanent tooth agenesis, excluding 3rd molars. Four hundred and fourt...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.02.020
更新日期:2017-07-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to investigate the relation between the remaining area of periodontal attachment and the attachment levels for each type of permanent teeth in a Chinese population by using micro-computed tomography (micro-CT) scans. DESIGN:A total of 440 extracted permanent teeth (including each t...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/j.archoralbio.2017.04.015
更新日期:2017-09-01 00:00:00
abstract::This study sought to localize immunohistochemically Janus kinase (Jak) and Tyk isoforms and STAT-1 in association with events involved in early dentine and enamel formation in the rat molar. The Jaks and STATs (signal transducers and activators of transcription) are key signal-transduction pathway components in the cy...
journal_title:Archives of oral biology
pub_type: 杂志文章
doi:10.1016/s0003-9969(96)00048-9
更新日期:1996-10-01 00:00:00