Identification of a risk dependent microRNA expression signature in myelodysplastic syndromes.


:The myelodysplastic syndromes (MDS) display both haematological and biological heterogeneity with variable leukaemia potential. MicroRNAs play an important role in tumour suppression and the regulation of self-renewal and differentiation of haematopoietic progenitors. Using a microarray platform, we evaluated microRNA expression from 44 patients with MDS and 17 normal controls. We identified a thirteen microRNA signature with statistically significant differential expression between normal and MDS specimens (P < 0·01), including down-regulation of members of the leukaemia-associated MIRLET7 family. A unique signature consisting of 10 microRNAs was closely associated with International Prognostic Scoring System (IPSS) risk category permitting discrimination between lower (Low/Intermediate-1) and higher risk (Intermediate-2/High) disease (P < 0·01). Selective overexpression of MIR181 family members was detected in higher risk MDS, indicating pathogenetic overlap with acute myeloid leukaemia. Survival analysis of an independent cohort of 22 IPSS lower risk MDS patients revealed a median survival of 3·5 years in patients with high expression of MIR181 family compared to 9·3 years in patients with low MIR181 expression (P = 0·002). Our pilot study suggested that analysis of microRNA expression profile offers diagnostic utility, and provide pathogenetic and prognostic discrimination in MDS.


Br J Haematol


Sokol L,Caceres G,Volinia S,Alder H,Nuovo GJ,Liu CG,McGraw K,Clark JA,Sigua CA,Chen DT,Moscinski L,Croce CM,List AF




Has Abstract


2011-04-01 00:00:00












  • Improvement of interleukin 2 production, clonogenic capability and restoration of stromal cell function in human immunodeficiency virus-type-1 patients after highly active antiretroviral therapy.

    abstract::Haematological abnormalities frequently occur in patients infected by human immunodeficiency virus-type 1 (HIV-1). Increasing evidence indicates that bone marrow suppression (BM) results from viral infection of accessory cells, with impaired stromal function and alteration of haematopoietic growth factor network. We h...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Isgrò A,Aiuti A,Mezzaroma I,Addesso M,Riva E,Giovannetti A,Mazzetta F,Alario C,Mazzone A,Ruco L,Aiuti F

    更新日期:2002-09-01 00:00:00

  • CD48 as a novel molecular target for antibody therapy in multiple myeloma.

    abstract::Monoclonal antibody (mAb) drugs are desirable for the improvement of multiple myeloma (MM) treatment. In this study, we found for the first time that CD48 was highly expressed on MM plasma cells. In 22 out of 24 MM patients, CD48 was expressed on more than 90% of MM plasma cells at significantly higher levels than it ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Hosen N,Ichihara H,Mugitani A,Aoyama Y,Fukuda Y,Kishida S,Matsuoka Y,Nakajima H,Kawakami M,Yamagami T,Fuji S,Tamaki H,Nakao T,Nishida S,Tsuboi A,Iida S,Hino M,Oka Y,Oji Y,Sugiyama H

    更新日期:2012-01-01 00:00:00

  • Theophylline, a new inducer of apoptosis in B-CLL: role of cyclic nucleotides.

    abstract::We report a case of indolent B-chronic lymphocytic leukaemia (B-CLL) in a stage A patient, treated for 10 years only by theophylline for bronchial asthma. As suggested by the spontaneous apoptosis in the patient's blood (10%), theophylline at 50 micrograms/ml increased spontaneous apoptosis after 72 h in culture by a ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Mentz F,Merle-Beral H,Ouaaz F,Binet JL

    更新日期:1995-08-01 00:00:00

  • Cytogenetic and molecular study of 32 Down syndrome families: potential leukaemia predisposing role of the most proximal segment of chromosome 21q.

    abstract::Down syndrome (DS) children have a 10-20-fold increased risk of developing ALL or AML compared to non-DS children. An increased disomic homozygosity of the polymorphic DNA markers in the pericentromeric region of chromosome 21q (21q11) has repeatedly been found in DS patients with ANLL-M7 and DS-specific transient abn...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Cavani S,Perfumo C,Argusti A,Pierluigi M,Perroni L,Schmiegelow K,Petersen MB,Cotter FE,Strigini P,Dagna-Bricarelli F,Nizetić D

    更新日期:1998-10-01 00:00:00

  • Acquired von Willebrand's disease due to aberrant expression of platelet glycoprotein Ib by marginal zone lymphoma cells.

    abstract::A 69-year-old woman presented with splenic marginal zone lymphoma associated with acquired von Willebrand's disease (AVWD). Laboratory abnormalities included markedly decreased plasma levels of factor VIII coagulant (C) activity (VIII:C 28%), von Willebrand's factor (VWF) antigen (Ag) (vWF:Ag < 6%), and VWF ristocetin...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Tefferi A,Hanson CA,Kurtin PJ,Katzmann JA,Dalton RJ,Nichols WL

    更新日期:1997-03-01 00:00:00

  • High prevalence of splenic marginal zone lymphoma among patients with acquired C1 inhibitor deficiency.

    abstract::Marginal zone lymphoma represents about 10% of all non-Hodgkin lymphomas (NHLs). 33% of patients with acquired angioedema (AAE) due to acquired C1-inhibitor (C1-INH) deficiency (C1-INH-AAE) have or will develop NHLs. C1-INH-AAE is a rare condition. We report the follow-up of 72 C1-INH-AAE patients, followed for a medi...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Castelli R,Wu MA,Arquati M,Zanichelli A,Suffritti C,Rossi D,Cicardi M

    更新日期:2016-03-01 00:00:00

  • Frequent lymphocytes infection by hepatitis B virus in haemophiliacs.

    abstract::We have tested the different mononuclear blood cell populations of seven patients with severe haemophilia and one patient with F VII deficiency for the presence of HBV DNA. These subjects were all polytransfused with non-heated coagulation factors; three were HBsAg positive, five HBsAg negative but anti-HBc and anti-H...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Lauré F,Chatenoud L,Pasquinelli C,Gazengel C,Beaurain G,Torchet MF,Zagury D,Bach JF,Bréchot C

    更新日期:1987-02-01 00:00:00

  • Bleeding time, blood groups and von Willebrand factor.

    abstract::The bleeding time in healthy volunteers was determined according to both the Ivy and the Simplate II techniques. A significantly longer bleeding time in people with blood group O than in people with non-O blood groups was demonstrated with both techniques. This difference could not be attributed to a difference in sex...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Caekebeke-Peerlinck KM,Koster T,Briët E

    更新日期:1989-10-01 00:00:00

  • Guideline: the laboratory diagnosis of malaria. General Haematology Task Force of the British Committee for Standards in Haematology.

    abstract::UK National External Quality Assessment Service surveys indicate continuing problems in malaria diagnosis: inaccurate calculation of parasitaemia or failure to estimate it altogether, difficulty distinguishing Plasmodium vivax from P. ovale, reporting malaria parasites when none were present and misidentification of P...

    journal_title:British journal of haematology

    pub_type: 信件,实务指引


    authors: Bailey JW,Williams J,Bain BJ,Parker-Williams J,Chiodini PL,General Haematology Task Force of the British Committee for Standards in Haematology.

    更新日期:2013-12-01 00:00:00

  • Deletions of the long arm of chromosome 7 in myeloid disorders: loss of band 7q32 implies worst prognosis.

    abstract::Clinical and cytogenetic data were analysed in 54 patients with acute non-lymphocytic leukaemias (ANLL) or MDS (myelodysplastic syndromes) and deletion of the long arm of chromosome 7 (7q-), in order to determine if there is a commonly deleted region in 7q and to establish possible correlations between karyotypic feat...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Velloso ER,Michaux L,Ferrant A,Hernandez JM,Meeus P,Dierlamm J,Criel A,Louwagie A,Verhoef G,Boogaerts M,Michaux JL,Bosly A,Mecucci C,Van den Berghe H

    更新日期:1996-03-01 00:00:00

  • Membrane proteins synthesized by human reticulocytes and their precursors.

    abstract::Membrane protein synthesis in human immature erythroid cells was studied by incubating the cells with 35S-methionine in vitro. The radioactive precursor amino acid was incorporated into membrane protein in a linear fashion for approximately 60 min, after which there was only a slight increase in incorporation. Intrace...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Krasnow SH,Pielichowski HJ,Caro J,Burka ER,Ballas SK

    更新日期:1981-02-01 00:00:00

  • Feasibility of prenatal diagnosis and carrier detection in South African haemophilia A patients.

    abstract::The feasibility of DNA diagnosis for haemophilia A was tested in South African patients and families by screening for the common inversion mutation in the factor VIII gene and for the intragenic microsatellite markers in introns 13 and 22. The allele frequencies at the two microsatellite loci were significantly differ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Dangerfield BT,Manga P,Field SP,Hartman E,Jenkins T,Krause A

    更新日期:1997-06-01 00:00:00

  • Bone marrow fibre production in myelofibrosis: a quantitative study.

    abstract::A digital image processing technique has been used to quantitate bone marrow fibre in histological material (both biopsy and necropsy) from 17 cases of myelofibrosis. An attempt was made to correlate the results of these measurements with other diagnostically relevant parameters. Bone marrow fibre was found to correla...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Bentley SA,Herman CJ

    更新日期:1979-05-01 00:00:00

  • Cytometry in monoclonal B-cell lymphocytosis and chronic lymphocytic leukaemia--the Hunting of the Snark?

    abstract::Cytometry has become important in the detection and determination of risk of monoclonal B-cell lymphocytosis; methodology has changed, and will continue to change, as cytometric technology changes. ...

    journal_title:British journal of haematology

    pub_type: 杂志文章,评审


    authors: Shapiro HM

    更新日期:2007-12-01 00:00:00

  • A left shift in the oxyhaemoglobin dissociation curve in patients with severe coronavirus disease 2019 (COVID-19).

    abstract::Critically ill patients with coronavirus disease 2019 (COVID-19) present with hypoxaemia and are mechanically ventilated to support gas exchange. We performed a retrospective, observational study of blood gas analyses (n = 3518) obtained from patients with COVID-19 to investigate changes in haemoglobin oxygen (Hb-O2 )...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Vogel DJ,Formenti F,Retter AJ,Vasques F,Camporota L

    更新日期:2020-11-01 00:00:00

  • BIOMED-2 PCR assays for IGK gene rearrangements are essential for B-cell clonality analysis in follicular lymphoma.

    abstract::B-cell clonality analysis is commonly performed by polymerase chain reaction (PCR) targeting the IGH genes although a high false-negative rate is recognized for germinal centre/post-germinal centre B-cell malignancies, especially follicular lymphoma. We assessed the diagnostic value of BIOMED-2 IGK assays and investig...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Payne K,Wright P,Grant JW,Huang Y,Hamoudi R,Bacon CM,Du MQ,Liu H

    更新日期:2011-10-01 00:00:00

  • Unbalanced X-chromosome inactivation in haemopoietic cells from normal women.

    abstract::We studied X-chromosome inactivation patterns in blood cells from normal females in three age groups: neonates (umbilical cord blood), 25-32 years old (young women group) and >75 years old (elderly women). Using PCR, the differential allele methylation status was evaluated on active and inactive X chromosomes at the h...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Tonon L,Bergamaschi G,Dellavecchia C,Rosti V,Lucotti C,Malabarba L,Novella A,Vercesi E,Frassoni F,Cazzola M

    更新日期:1998-09-01 00:00:00

  • Marked decrease in the Epstein-Barr virus positivity rate in nodular sclerosis subtype Hodgkin's disease in Tokyo: trend between 1955 and 1999.

    abstract::We studied the trends of the Epstein-Barr virus (EBV) positivity rate in Hodgkin's disease (HD) between 1955 and 1999. The overall positivity rate gradually decreased over time [12 out of 23 (52%) in 1955-69; 18 out of 39 (46%) in 1970-84; 13 out of 44 (35%) in 1985-99; P = 0.135]. Interestingly, a marked decrease in ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Takeuchi K,Morishita Y,Fukayama M,Mori S

    更新日期:2001-05-01 00:00:00

  • The impact of SOCS1 mutations in diffuse large B-cell lymphoma.

    abstract::Mutations in SOCS1 are frequent in primary mediastinal B-cell lymphoma and classical Hodgkin lymphoma. In the latter, SOCS1 mutations affect the length of the encoded protein (major mutations) and are associated with shorter patient survival. Two independent studies examined the prognostic impact of SOCS1 mutations in...

    journal_title:British journal of haematology

    pub_type: 杂志文章,随机对照试验


    authors: Mellert K,Martin M,Lennerz JK,Lüdeke M,Staiger AM,Kreuz M,Löffler M,Schmitz N,Trümper L,Feller AC,Hartmann S,Hansmann ML,Klapper W,Stein H,Rosenwald A,Ott G,Ziepert M,Möller P

    更新日期:2019-12-01 00:00:00

  • Production of immunoreactive calcitonin by myeloid leukaemia cells.

    abstract::Raised plasma levels of immunoreactive human calcitonin (i-HCT) have been found in patients with chronic granulocytic leukaemia (CGL) in chronic phase and myeloblastic transformation and in patients with acute myeloid leukaemia at presentation and in relapse. In CGL levels were significantly higher in myeloblastic tra...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Foa R,Oscier DG,Hillyard CJ,Incarbone E,McIntyre I,Goldman JM

    更新日期:1982-02-01 00:00:00

  • Risk factors for pericardial effusion in adult patients receiving allogeneic haematopoietic stem cell transplantation.

    abstract::Pericardial effusion (PE) is a rare but potentially life-threatening complication for allogeneic haematopoietic stem cell transplantation (HSCT) recipients. The risk factors, aetiology, incidence and therapy are largely unclear. To investigate this issue, we reviewed 391 adult patients undergoing allogeneic HSCT betwe...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Liu YC,Chien SH,Fan NW,Hu MH,Gau JP,Liu CJ,Yu YB,Liu CY,Hsiao LT,Liu JH,Chiou TJ,Tzeng CH

    更新日期:2015-06-01 00:00:00

  • Prophylactic effect of recombinant factor VIIa in factor VII deficient patients.

    abstract::Inherited factor VII (FVII) deficiency is a rare autosomal recessive disorder associated with a bleeding tendency. We describe three patients with congenital FVII deficiency who have been treated with activated recombinant factor VII (rVIIa). Two patients had novel mutations and were treated prophylactically with 1.2 ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Mathijssen NC,Masereeuw R,Verbeek K,Lavergne JM,Costa JM,van Heerde WL,Nováková IR

    更新日期:2004-05-01 00:00:00

  • In vitro and in vivo effects of treatment by platelet-activating factor on N-formyl-met-leu-phe-mediated responses of polymorphonuclear leucocytes.

    abstract::Two chemoattractants, the peptide N-formyl-met-leu-phe (FMLP), and the ether phospholipid, platelet activating factor (PAF), each stimulate a variety of in vitro responses in polymorphonuclear leucocytes (PMN). Because often more than one inflammatory mediator is active during inflammation, we determined the effect on...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Ingraham LM,Lafuze JE,Boxer LA,Baehner RL

    更新日期:1987-06-01 00:00:00

  • In vitro cytotoxicity of nelarabine, clofarabine and flavopiridol in paediatric acute lymphoblastic leukaemia.

    abstract::The in vitro efficacies of three new drugs--clofarabine (CLOF), nelarabine (NEL) and flavopiridol (FP) - were assessed in a panel of acute lymphoblastic leukaemia (ALL) cell lines. The 50% inhibitory concentration (IC50) for CLOF across all lines was 188-fold lower than that of NEL. B-lineage, but not T-lineage lines,...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Beesley AH,Palmer ML,Ford J,Weller RE,Cummings AJ,Freitas JR,Firth MJ,Perera KU,de Klerk NH,Kees UR

    更新日期:2007-04-01 00:00:00

  • Expression of bcr-abl mRNA in individual chronic myelogenous leukaemia cells as determined by in situ amplification.

    abstract::We present the results of a novel method developed for evaluation of in situ amplification, a molecular genetic method at the cellular level. Reverse transcription polymerase chain reaction (RT-PCR) was used to study bcr-abl transcript levels in individual cells from patients with chronic myelogenous leukaemia (CML). ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Pachmann K,Zhao S,Schenk T,Kantarjian H,El-Naggar AK,Siciliano MJ,Guo JQ,Arlinghaus RB,Andreeff M

    更新日期:2001-03-01 00:00:00

  • Megakaryocytic colony formation in polycythaemia vera and secondary erythrocytosis.

    abstract::Megakaryocytic colony formation by progenitor cells of 18 patients with polycythaemia vera, seven with secondary erythrocytosis and four with erythrocytosis of unexplained origin was studied in vitro by the methyl cellulose culture assay. Fourteen of the 18 patients with polycythaemia vera showed spontaneous megakaryo...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Juvonen E,Partanen S,Ikkala E,Ruutu T

    更新日期:1988-08-01 00:00:00

  • Impaired immune responses to herpesviruses and microbial ligands in patients with MonoMAC.

    abstract::MonoMAC is a complex primary immunodeficiency caused by mutations in the myeloid transcription factor GATA2, characterized by multilineage cytopenia with malignant complications and severe infections, including mycobacteria and herpesviruses. We describe the clinical presentation, genetics and antiviral inflammatory r...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Mardahl M,Jørgensen SE,Schneider A,Raaschou-Jensen K,Holm M,Veirum J,Kristensen TK,Johansen IS,Christiansen M,Assing K,Mogensen TH

    更新日期:2019-08-01 00:00:00

  • Recent advances in the understanding of genetic defects of neutrophil number and function.

    abstract::Neutrophils are amongst the first immune cells to arrive at sites of infection and play an important role as the host's first line of defence against invading pathogens. Defects of neutrophil number or function are usually recognized clinically by recurrent infections that often are life-threatening. Over the last few...

    journal_title:British journal of haematology

    pub_type: 杂志文章,评审


    authors: Bouma G,Ancliff PJ,Thrasher AJ,Burns SO

    更新日期:2010-11-01 00:00:00

  • Inhibition of platelet function with 2,3-dihydroxybenzoic acid.

    abstract::2,3-Dihydroxybenzoic acid (2,3-DHB) inhibits the second wave of platelet aggregation release of serotonin, and malonaldehyde production. The effect is concentration dependent and reversible in vitro and in vivo. 50% inhibition of platelet aggregation induced in vitro with 70 micron adrenaline was obtained with 1.5 mM ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Greenberg M,Grady RW,Peterson CM

    更新日期:1977-12-01 00:00:00

  • alpha-globin gene deletions associated with Hb J Tongariki.

    abstract::Three identical alpha + thalassemia genes, one of which always carried the Hb J Tongariki mutation, have been observed in Vanuatuans. Despite the fact that at least two of them have arisen by different types of crossover event, the expression of all three haplotypes is identical. ...

    journal_title:British journal of haematology

    pub_type: 杂志文章


    authors: Bowden DK,Pressley L,Higgs DR,Clegg JB,Weatherall DJ

    更新日期:1982-06-01 00:00:00