A low frequency of pancreatic islet insulin-expressing cells derived from cord blood stem cell allografts in humans.


AIMS/HYPOTHESIS:We sought to establish if stem cells contained in cord blood cell allografts have the capacity to differentiate into insulin-expressing beta cells in humans. METHODS:We studied pancreases obtained at autopsy from individuals (n = 11) who had prior opposite-sex cord blood transplants to reconstitute haematopoiesis. Pancreatic tissue sections were stained first by XY-fluorescence in situ hybridisation and then insulin immunohistochemistry. Pancreases obtained at autopsy from participants without cord blood cell infusions served as controls (n = 11). RESULTS:In the men with prior transplant of female cord blood, there were 3.4 ± 0.3% XX-positive insulin-expressing islet cells compared with 0.32 ± 0.05% (p < 0.01) in male controls. In women with prior transplant of male cord blood cells we detected 1.03 ± 0.20% XY insulin-expressing islet cells compared with 0.03 ± 0.03 in female controls (p < 0. 001). CONCLUSIONS/INTERPRETATION:Cord blood stem cells have the capacity to differentiate into insulin-expressing cells in non-diabetic humans. It remains to be established whether these cells have the properties of beta cells.






Huang CJ,Butler AE,Moran A,Rao PN,Wagner JE,Blazar BR,Rizza RA,Manivel JC,Butler PC




Has Abstract


2011-05-01 00:00:00












  • Differential binding of sulphated insulin to adipocytes and hepatocytes.

    abstract::The polymerization and precipitation of highly purified insulins which causes major problems in portable infusion systems does not occur with sulphated insulin. To compare the biological behaviour of sulphated insulin with that of a neutral highly purified monocomponent insulin, insulin receptor studies were performed...


    pub_type: 杂志文章


    authors: Zeuzem S,Taylor R,Agius L,Albisser AM,Alberti KG

    更新日期:1984-08-01 00:00:00

  • Multiple metabolic abnormalities in normal glucose tolerant relatives of NIDDM families.

    abstract::Non-diabetic first degree relatives of non-insulin-dependent diabetic (NIDDM) families are at increased risk of developing diabetes mellitus, and have been studied to identify early metabolic abnormalities. Hormone concentrations measured by specific enzyme immunoassays were assessed in non-diabetic relatives of North...


    pub_type: 杂志文章


    authors: Humphriss DB,Stewart MW,Berrish TS,Barriocanal LA,Trajano LR,Ashworth LA,Brown MD,Miller M,Avery PJ,Alberti KG,Walker M

    更新日期:1997-10-01 00:00:00

  • The response of gastric inhibitory polypeptide (GIP) and insulin to glucose in duodenal ulcer patients.

    abstract::The response of Gastric Inhibitory Polypeptide (GIP) and insulin to a 50 g oral glucose tolerance test (OGTT) and an intravenous glucose infusion (IVGI), which copied the changes in plasma glucose concentrations during the OGTT, were measured in 10 patients with duodenal ulcer and in 10 healthy control subjects. The m...


    pub_type: 杂志文章


    authors: Lauritsen KB,Moody AJ

    更新日期:1978-03-01 00:00:00

  • Mast cells and type VIII collagen in human diabetic nephropathy.

    abstract::Renal injury in diabetes mellitus is associated with progressive interstitial fibrosis and extracellular matrix accumulation. However, the phenotypes of cells forming the interstitial infiltrate in diabetic nephropathy have not been precisely defined. There is increasing evidence for the association of mast cells with...


    pub_type: 杂志文章


    authors: Rüger BM,Hasan Q,Greenhill NS,Davis PF,Dunbar PR,Neale TJ

    更新日期:1996-10-01 00:00:00

  • Insulin degradation. XXVIII. Immunocytochemical localization of glutathione-insulin transhydrogenase in the pancreas, kidney and liver of normal and streptozotocin-diabetic rats and of lean and obese (ob/ob) mice.

    abstract::Glutathione-insulin transhydrogenase catalyzed the inactivation of insulin by splitting the hormone into A and B chains. We have localized this enzyme immunocytochemically by light microscopy in the pancreas, kidney and liver of both lean and obese (ob/ob) mice and similarly in normal and streptozotocin-diabetic rats....


    pub_type: 杂志文章


    authors: Taylor CA,Varandani PT

    更新日期:1981-11-01 00:00:00

  • C3H/HeJ mice carrying a toll-like receptor 4 mutation are protected against the development of insulin resistance in white adipose tissue in response to a high-fat diet.

    abstract:AIMS/HYPOTHESIS:Inflammation is associated with obesity and has been implicated in the development of diabetes and atherosclerosis. During gram-negative bacterial infection, lipopolysaccharide causes an inflammatory reaction via toll-like receptor 4 (TLR4), which has an essential function in the induction of innate and...


    pub_type: 杂志文章


    authors: Poggi M,Bastelica D,Gual P,Iglesias MA,Gremeaux T,Knauf C,Peiretti F,Verdier M,Juhan-Vague I,Tanti JF,Burcelin R,Alessi MC

    更新日期:2007-06-01 00:00:00

  • Accumulation of autoreactive effector T cells and allo-specific regulatory T cells in the pancreas allograft of a type 1 diabetic recipient.

    abstract:AIMS/HYPOTHESIS:Simultaneous kidney-pancreas transplantation is an established treatment for patients with type 1 diabetes and end-stage renal failure, even though restored beta cell function may become affected by recurrent islet autoimmunity or graft rejection. We characterised infiltrating lymphocytes isolated from ...


    pub_type: 杂志文章


    authors: Velthuis JH,Unger WW,van der Slik AR,Duinkerken G,Engelse M,Schaapherder AF,Ringers J,van Kooten C,de Koning EJ,Roep BO

    更新日期:2009-03-01 00:00:00

  • Paradoxical glucagon response after stimulation with glucose and arginine in isolated pancreatic sand rat islets.

    abstract::Isolated pancreatic islets of normoglycemic sand rats do not respond to 2.5 mM glucose with an enhanced glucagon secretion, which could be observed in normal Wistar rats. Arginine stimulates glucagon release in the presence of 2.5 mM glucose in Wistar rats as well as in sand rats. The secretion pattern is not caused b...


    pub_type: 杂志文章


    authors: Ziegler M,Hahn HJ,Ziegler B,Köhler E,Fiedler H

    更新日期:1975-02-01 00:00:00

  • Assessment of urinary microparticles in normotensive patients with type 1 diabetes.

    abstract:AIMS/HYPOTHESIS:Assessment of urinary extracellular vesicles including exosomes and microparticles (MPs) is an emerging approach for non-invasive detection of renal injury. We have previously reported that podocyte-derived MPs are increased in diabetic mice in advance of albuminuria. Here, we hypothesised that type 1 d...


    pub_type: 杂志文章


    authors: Lytvyn Y,Xiao F,Kennedy CR,Perkins BA,Reich HN,Scholey JW,Cherney DZ,Burger D

    更新日期:2017-03-01 00:00:00

  • The effect of insulin on renal sodium metabolism. A review with clinical implications.

    abstract::Data are discussed which demonstrate that insulin plays an important role in sodium metabolism. The primary action of insulin on sodium balance is exerted on the kidney. Increases in plasma insulin concentration within the physiological range stimulate sodium reabsorption by the distal nephron segments and this effect...


    pub_type: 杂志文章,评审


    authors: DeFronzo RA

    更新日期:1981-09-01 00:00:00

  • Leptin resistance exacerbates diet-induced obesity and is associated with diminished maximal leptin signalling capacity in rats.

    abstract:AIMS/HYPOTHESIS:Leptin resistance is generally considered a consequence of obesity. We postulated that leptin resistance is associated with diminished hypothalamic leptin signalling capacity and that leptin resistance is causal to obesity. We assessed maximal leptin-mediated binding of the transcription factor signal t...


    pub_type: 杂志文章


    authors: Scarpace PJ,Matheny M,Tümer N,Cheng KY,Zhang Y

    更新日期:2005-06-01 00:00:00

  • Multiple defects of both hepatic and peripheral intracellular glucose processing contribute to the hyperglycaemia of NIDDM.

    abstract::Non-insulin-dependent diabetic (NIDDM) patients were studied during a modified euglycaemic state when fasting hyperglycaemia was normalized by a prior (-210 to -150 min)--and later withdrawn (-150-0 min)--intravenous insulin infusion. Glucose metabolism was assessed in NIDDM patients (n = 10) and matched control subje...


    pub_type: 杂志文章


    authors: Vaag A,Alford F,Henriksen FL,Christopher M,Beck-Nielsen H

    更新日期:1995-03-01 00:00:00

  • Silencing Jnk1 and Jnk2 accelerates basal lipolysis and promotes fatty acid re-esterification in mouse adipocytes.

    abstract:AIMS/HYPOTHESIS:Elevated plasma levels of NEFA impair insulin action. Given the positive linear correlation between NEFA released by adipocytes and plasma NEFA levels, identification of mechanisms controlling adipocyte lipolysis and NEFA release could provide a guide to new therapies for insulin resistance and type 2 d...


    pub_type: 杂志文章


    authors: Rozo AV,Vijayvargia R,Weiss HR,Ruan H

    更新日期:2008-08-01 00:00:00

  • Diabetes in pregnancy 1985.

    abstract::The art of obstetrics is not a subject which is often discussed in the pages of Diabetologia. However, as the care of the diabetic mother and her offspring is rightly an interdisciplinary responsibility between obstetrician, diabetologist and neonatologist, it is important that each has a close understanding of the va...


    pub_type: 杂志文章,评审


    authors: Hadden DR

    更新日期:1986-01-01 00:00:00

  • Effect of furosemide on insulin and glucagon responses to arginine in normal subjects.

    abstract::This study aimed at evaluating the influence of furosemide upon insulin and glucagon responses to arginine in healthy subjects. For this purpose, six normal subjects received two consecutive arginine pulses (3 g), 60 min apart, before and after the administration of furosemide (40 mg, IV). The acute insulin response (...


    pub_type: 杂志文章


    authors: Giugliano D,Torella R,Sgambato S,D'Onofrio F

    更新日期:1980-04-01 00:00:00

  • Discordant gene expression in skeletal muscle and adipose tissue of patients with type 2 diabetes: effect of interleukin-6 infusion.

    abstract:AIMS/HYPOTHESIS:We compared metabolic gene expression in adipose tissue and skeletal muscle from patients with type 2 diabetes and from well-matched healthy control subjects. We hypothesised that gene expression would be discordantly regulated when comparing the two groups. Our secondary aim was to determine the effect...


    pub_type: 杂志文章


    authors: Carey AL,Petersen EW,Bruce CR,Southgate RJ,Pilegaard H,Hawley JA,Pedersen BK,Febbraio MA

    更新日期:2006-05-01 00:00:00

  • Modulation of insulin receptor signalling: significance of altered receptor isoform patterns and mechanism of hyperglycaemia-induced receptor modulation.

    abstract::Insulin resistance of the skeletal muscle plays a key role in the development of the metabolic endocrine syndrome and its further progression to non-insulin dependent diabetes (NIDDM). Available data suggest that insulin resistance is caused by an impaired signal from the insulin receptor to the glucose transport syst...


    pub_type: 传,历史文章,杂志文章,评审


    authors: Häring HU,Kellerer M,Mosthaf L

    更新日期:1994-09-01 00:00:00

  • Glucose-induced Akt1 activation mediates fibronectin synthesis in endothelial cells.

    abstract:AIMS/HYPOTHESIS:Increased expression and decreased degradation of extracellular matrix (ECM) proteins are key features of chronic diabetic complications. Fibronectin, a predominant ECM protein, has been shown to be overexpressed in all target organs of diabetic complications and in endothelial cells cultured in high le...


    pub_type: 杂志文章


    authors: Xin X,Khan ZA,Chen S,Chakrabarti S

    更新日期:2005-11-01 00:00:00

  • Weight tracking in childhood and adolescence and type 2 diabetes risk.

    abstract:AIMS/HYPOTHESIS:The aim of this work was to examine the associations of average weight and weight velocity in three growth periods from birth through adolescence with type 2 diabetes incidence. METHODS:Child participants were selected from a 43 year longitudinal study of American Indians to represent three growth peri...


    pub_type: 杂志文章


    authors: Olaiya MT,Knowler WC,Sinha M,Kobes S,Nelson RG,Baier LJ,Muller YL,Hanson RL

    更新日期:2020-09-01 00:00:00

  • Increased prevalence of non-alcoholic fatty liver disease in European women with a history of gestational diabetes.

    abstract:AIMS/HYPOTHESIS:Non-alcoholic fatty liver disease (NAFLD) is common in type 2 diabetes but it is unknown whether NAFLD is prevalent in European women at risk of type 2 diabetes. We studied the prevalence of, and risk factors for, NAFLD in European women with previous gestational diabetes (GDM) at high risk of type 2 di...


    pub_type: 杂志文章


    authors: Forbes S,Taylor-Robinson SD,Patel N,Allan P,Walker BR,Johnston DG

    更新日期:2011-03-01 00:00:00

  • Similar progression of diabetic retinopathy with insulin glargine and neutral protamine Hagedorn (NPH) insulin in patients with type 2 diabetes: a long-term, randomised, open-label study.

    abstract:AIMS/HYPOTHESIS:This long-term study was designed to further characterise the retinal safety profile of insulin glargine and human neutral protamine Hagedorn (NPH) insulin in patients with type 2 diabetes mellitus. METHODS:An open-label, 5 year, randomised (1:1), multicentre, stratified, parallel-group study conducted...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Rosenstock J,Fonseca V,McGill JB,Riddle M,Hallé JP,Hramiak I,Johnston P,Davis M

    更新日期:2009-09-01 00:00:00

  • Risk stratification systems for diabetic foot ulcers: a systematic review.

    abstract:AIMS/HYPOTHESIS:Several risk stratification systems have been proposed for predicting development of diabetic foot ulcer. However, little has been published that assesses their similarities and disparities, diagnostic accuracy and evidence level. Consequently, we conducted a systematic review of the existing stratifica...


    pub_type: 杂志文章,评审


    authors: Monteiro-Soares M,Boyko EJ,Ribeiro J,Ribeiro I,Dinis-Ribeiro M

    更新日期:2011-05-01 00:00:00

  • In vitro kinetics of insulin release by microencapsulated rat islets: effect of the size of the microcapsules.

    abstract::Microencapsulation has been proposed to protect islets of Langerhans against immune rejection in xenogenic transplantation. However, to achieve glucose homeostasis in human diabetic patients, insulin release by microencapsulated islets must increase in response to a glucose load. We microencapsulated isolated rat isle...


    pub_type: 杂志文章


    authors: Chicheportiche D,Reach G

    更新日期:1988-01-01 00:00:00

  • G protein-coupled receptor (GPR)40-dependent potentiation of insulin secretion in mouse islets is mediated by protein kinase D1.

    abstract:AIMS/HYPOTHESIS:Activation of the G protein-coupled receptor (GPR)40 by long-chain fatty acids potentiates glucose-stimulated insulin secretion (GSIS) from pancreatic beta cells, and GPR40 agonists are in clinical development for type 2 diabetes therapy. GPR40 couples to the G protein subunit Gα(q/11) but the signallin...


    pub_type: 杂志文章


    authors: Ferdaoussi M,Bergeron V,Zarrouki B,Kolic J,Cantley J,Fielitz J,Olson EN,Prentki M,Biden T,MacDonald PE,Poitout V

    更新日期:2012-10-01 00:00:00

  • Report from the International Pancreas Transplant Registry.

    abstract::From December, 1966 through 1990, 3082 pancreas transplants were reported to the International Registry. A detailed analysis was performed on the 2087 transplants in the 1986-90 data base; during this time the overall one year recipient and graft functional survival rates were 89% and 62%. Univariate analyses showed g...


    pub_type: 杂志文章


    authors: Sutherland DE

    更新日期:1991-08-01 00:00:00

  • Association between diabetes and haematological malignancies: a population-based study.

    abstract:AIMS/HYPOTHESIS:Contemporary data for the association of diabetes with haematological malignancies are lacking. We evaluated the risk of developing haematological malignancies and subsequent mortality in individuals with diabetes compared with those without diabetes. METHODS:We conducted a population-based observation...


    pub_type: 杂志文章


    authors: Gong IY,Cheung MC,Read S,Na Y,Lega IC,Lipscombe LL

    更新日期:2021-01-06 00:00:00

  • Antioxidant treatment of experimental diabetic retinopathy in rats with nicanartine.

    abstract::In order to study the contribution of oxidant stress to the pathogenesis of experimental diabetic retinopathy, male streptozotocin diabetic Lewis rats were treated with the antioxidant and lipid-lowering compound nicanartine (80 mg/kg; n = 8, blood glucose level 16.7 +/- 3.9 mmol/l; HbA1 11.8 +/- 1.6%) by oral supplem...


    pub_type: 杂志文章


    authors: Hammes HP,Bartmann A,Engel L,Wülfroth P

    更新日期:1997-06-01 00:00:00

  • Retinopathy in old persons with and without diabetes mellitus: the Age, Gene/Environment Susceptibility--Reykjavik Study (AGES-R).

    abstract:AIMS/HYPOTHESIS:We aimed to describe the prevalence of retinopathy in an aged cohort of Icelanders with and without diabetes mellitus. METHODS:The study population consisted of 4,994 persons aged ≥ 67 years, who participated in the Age, Gene/Environment Susceptibility-Reykjavik Study (AGES-R). Type 2 diabetes mellitus...


    pub_type: 杂志文章


    authors: Gunnlaugsdottir E,Halldorsdottir S,Klein R,Eiriksdottir G,Klein BE,Benediktsson R,Harris TB,Launer LJ,Aspelund T,Gudnason V,Cotch MF,Jonasson F

    更新日期:2012-03-01 00:00:00

  • Autoantibodies to nervous tissue structures are associated with autonomic neuropathy in type 1 (insulin-dependent) diabetes mellitus.

    abstract::There is evidence that the immune system may play a role in the pathogenesis of autonomic neuropathy in Type 1 (insulin-dependent) diabetes mellitus. In the present study, we investigated the presence of autoantibodies to sympathetic and parasympathetic nervous structures and their correlation with other conventional ...


    pub_type: 杂志文章


    authors: Zanone MM,Peakman M,Purewal T,Watkins PJ,Vergani D

    更新日期:1993-06-01 00:00:00

  • Effects of streptozotocin-induced diabetes and noradrenaline infusion on cardiac output and its regional distribution in pithed rats.

    abstract::46Sc-and 99mTc-labelled microspheres were used to measure the effects of noradrenaline infusion on cardiac output and its regional distribution in 10 control and 10 streptozotocin-diabetic pithed rats. Plasma noradrenaline concentrations during the infusion were similar in both groups. Pressor responses were significa...


    pub_type: 杂志文章


    authors: Lucas PD

    更新日期:1985-02-01 00:00:00