A low frequency of pancreatic islet insulin-expressing cells derived from cord blood stem cell allografts in humans.


AIMS/HYPOTHESIS:We sought to establish if stem cells contained in cord blood cell allografts have the capacity to differentiate into insulin-expressing beta cells in humans. METHODS:We studied pancreases obtained at autopsy from individuals (n = 11) who had prior opposite-sex cord blood transplants to reconstitute haematopoiesis. Pancreatic tissue sections were stained first by XY-fluorescence in situ hybridisation and then insulin immunohistochemistry. Pancreases obtained at autopsy from participants without cord blood cell infusions served as controls (n = 11). RESULTS:In the men with prior transplant of female cord blood, there were 3.4 ± 0.3% XX-positive insulin-expressing islet cells compared with 0.32 ± 0.05% (p < 0.01) in male controls. In women with prior transplant of male cord blood cells we detected 1.03 ± 0.20% XY insulin-expressing islet cells compared with 0.03 ± 0.03 in female controls (p < 0. 001). CONCLUSIONS/INTERPRETATION:Cord blood stem cells have the capacity to differentiate into insulin-expressing cells in non-diabetic humans. It remains to be established whether these cells have the properties of beta cells.






Huang CJ,Butler AE,Moran A,Rao PN,Wagner JE,Blazar BR,Rizza RA,Manivel JC,Butler PC




Has Abstract


2011-05-01 00:00:00












  • The effect of hyperglycaemia on pancreatic islets transplanted into rats beneath the kidney capsule.

    abstract::The effect of hyperglycaemia on islet transplantation in the rat has been examined in two ways, using syngeneic transplantation of 400 islets to the kidney capsule and subsequent measurement of kidney insulin content as a measurement of B-cell survival. Firstly, islets were transplanted into either diabetic or normal ...


    pub_type: 杂志文章


    authors: Gray DW,Cranston D,McShane P,Sutton R,Morris PJ

    更新日期:1989-09-01 00:00:00

  • A method for the purification of single A, B and D cells and for the isolation of coupled cells from isolated rat islets.

    abstract::A method has been developed for the purification of single A, B and D cells and for the isolation of coupled islet cells. Isolated rat islets were dissociated by repeated pipetting in the presence of trypsin and ethylene glycol bis (beta-aminoethyl ether) N,N' tetra-acetic acid (EGTA), and then filtered through nylon ...


    pub_type: 杂志文章


    authors: Pipeleers DG,Pipeleers-Marichal MA

    更新日期:1981-06-01 00:00:00

  • Adults with type 1 diabetes eat a high-fat atherogenic diet that is associated with coronary artery calcium.

    abstract:AIMS/HYPOTHESIS:Coronary heart disease is the leading cause of mortality among people with type 1 diabetes. Diet is an important lifestyle factor that relates to risk of CHD. The aim of this study was to examine how diet and adherence to dietary guidelines differ between adults with and without type 1 diabetes, and the...


    pub_type: 杂志文章


    authors: Snell-Bergeon JK,Chartier-Logan C,Maahs DM,Ogden LG,Hokanson JE,Kinney GL,Eckel RH,Ehrlich J,Rewers M

    更新日期:2009-05-01 00:00:00

  • Characterising nitric oxide-mediated metabolic benefits of low-dose ultraviolet radiation in the mouse: a focus on brown adipose tissue.

    abstract:AIMS/HYPOTHESIS:Exposure to sunlight has the potential to suppress metabolic dysfunction and obesity. We previously demonstrated that regular exposure to low-doses of ultraviolet radiation (UVR) reduced weight gain and signs of diabetes in male mice fed a high-fat diet, in part via release of nitric oxide from skin. He...


    pub_type: 杂志文章


    authors: Dhamrait GK,Panchal K,Fleury NJ,Abel TN,Ancliffe MK,Crew RC,Croft K,Fernandez BO,Minnion M,Hart PH,Lucas RM,Mark PJ,Feelisch M,Weller RB,Matthews V,Gorman S

    更新日期:2020-01-01 00:00:00

  • Taq I polymorphism of the vitamin D receptor and risk of severe diabetic retinopathy.

    abstract:AIMS/HYPOTHESIS:Vitamin D, a molecule with antiproliferative, antiangiogenic, antioxidant and immunosuppressive effects, could play a role in the pathogenesis of severe diabetic retinopathy. We examined whether Taq I polymorphism of the vitamin D receptor is involved in the development of severe diabetic retinopathy. ...


    pub_type: 杂志文章


    authors: Taverna MJ,Sola A,Guyot-Argenton C,Pacher N,Bruzzo F,Slama G,Reach G,Selam JL

    更新日期:2002-03-01 00:00:00

  • Regulation of insulin sensitivity by serine/threonine phosphorylation of insulin receptor substrate proteins IRS1 and IRS2.

    abstract::The insulin receptor substrate proteins IRS1 and IRS2 are key targets of the insulin receptor tyrosine kinase and are required for hormonal control of metabolism. Tissues from insulin-resistant and diabetic humans exhibit defects in IRS-dependent signalling, implicating their dysregulation in the initiation and progre...


    pub_type: 杂志文章,评审


    authors: Copps KD,White MF

    更新日期:2012-10-01 00:00:00

  • Risk stratification systems for diabetic foot ulcers: a systematic review.

    abstract:AIMS/HYPOTHESIS:Several risk stratification systems have been proposed for predicting development of diabetic foot ulcer. However, little has been published that assesses their similarities and disparities, diagnostic accuracy and evidence level. Consequently, we conducted a systematic review of the existing stratifica...


    pub_type: 杂志文章,评审


    authors: Monteiro-Soares M,Boyko EJ,Ribeiro J,Ribeiro I,Dinis-Ribeiro M

    更新日期:2011-05-01 00:00:00

  • Metformin and the gastrointestinal tract.

    abstract::Metformin is an effective agent with a good safety profile that is widely used as a first-line treatment for type 2 diabetes, yet its mechanisms of action and variability in terms of efficacy and side effects remain poorly understood. Although the liver is recognised as a major site of metformin pharmacodynamics, rece...


    pub_type: 杂志文章,评审


    authors: McCreight LJ,Bailey CJ,Pearson ER

    更新日期:2016-03-01 00:00:00

  • The limited storage capacity of gonadal adipose tissue directs the development of metabolic disorders in male C57Bl/6J mice.

    abstract:AIMS/HYPOTHESIS:White adipose tissue (WAT) consists of various depots with different adipocyte functionality and immune cell composition. Knowledge of WAT-depot-specific differences in expandability and immune cell influx during the development of obesity is limited, therefore we aimed to characterise different WAT dep...


    pub_type: 杂志文章


    authors: van Beek L,van Klinken JB,Pronk AC,van Dam AD,Dirven E,Rensen PC,Koning F,Willems van Dijk K,van Harmelen V

    更新日期:2015-07-01 00:00:00

  • Differential binding of sulphated insulin to adipocytes and hepatocytes.

    abstract::The polymerization and precipitation of highly purified insulins which causes major problems in portable infusion systems does not occur with sulphated insulin. To compare the biological behaviour of sulphated insulin with that of a neutral highly purified monocomponent insulin, insulin receptor studies were performed...


    pub_type: 杂志文章


    authors: Zeuzem S,Taylor R,Agius L,Albisser AM,Alberti KG

    更新日期:1984-08-01 00:00:00

  • Eighteen year weight trajectories and metabolic markers of diabetes in modernising China.

    abstract:AIMS/HYPOTHESIS:Although obesity is a major risk factor for diabetes, little is known about weight gain trajectories across adulthood, and whether they are differentially associated with metabolic markers of diabetes. METHODS:We used fasting blood samples and longitudinal weight data for 5,436 adults (5,734 observatio...


    pub_type: 杂志文章


    authors: Gordon-Larsen P,Koehler E,Howard AG,Paynter L,Thompson AL,Adair LS,Mayer-Davis EJ,Zhang B,Popkin BM,Herring AH

    更新日期:2014-09-01 00:00:00

  • Effect of phenformin on hepatic balances of gluconeogenic substrates in man.

    abstract::The effect of a five day pretreatment with phenformin (3 X 50 mg daily) on hepatic metabolism was studied in six healthy volunteers. Arterial and hepatic venous concentrations of substrates and hepatic blood flow were estimated during a basal period and during a low-dose lactate infusion (0,03 mmol . kg-1 . min-1). Th...


    pub_type: 杂志文章


    authors: Dietze G,Wicklmayr M,Mehnert H,Czempiel H,Henftling HG

    更新日期:1978-04-01 00:00:00

  • Reduced plasma adiponectin concentrations may contribute to impaired insulin activation of glycogen synthase in skeletal muscle of patients with type 2 diabetes.

    abstract:AIMS/HYPOTHESIS:Circulating levels of adiponectin are negatively associated with multiple indices of insulin resistance, and the concentration is reduced in humans with insulin resistance and type 2 diabetes. However, the mechanisms by which adiponectin improves insulin sensitivity remain unclear. SUBJECTS AND METHODS...


    pub_type: 杂志文章


    authors: Højlund K,Frystyk J,Levin K,Flyvbjerg A,Wojtaszewski JF,Beck-Nielsen H

    更新日期:2006-06-01 00:00:00

  • Bivariate genome-wide scan for metabolic phenotypes in non-diabetic Chinese individuals from the Stanford, Asia and Pacific Program of Hypertension and Insulin Resistance Family Study.

    abstract:AIMS/HYPOTHESIS:Hypertension, obesity, impaired glucose tolerance and dyslipidaemia are metabolic abnormalities that often cluster together more often than expected by chance alone. Since these metabolic variables are highly heritable and are at least partially genetically determined, the clustering of defects in these...


    pub_type: 杂志文章


    authors: Chiu YF,Chuang LM,Kao HY,Ho LT,Ting CT,Hung YJ,Chen YD,Donlon T,Curb JD,Quertermous T,Hsiung CA

    更新日期:2007-08-01 00:00:00

  • Dissociation of lipotoxicity and glucotoxicity in a mouse model of obesity associated diabetes: role of forkhead box O1 (FOXO1) in glucose-induced beta cell failure.

    abstract:AIMS/HYPOTHESIS:Carbohydrate-free diet prevents hyperglycaemia and beta cell destruction in the New Zealand Obese (NZO) mouse model. Here we have used a sequential dietary regimen to dissociate the effects of obesity and hyperglycaemia on beta cell function and integrity, and to study glucose-induced alterations of key...


    pub_type: 杂志文章


    authors: Kluth O,Mirhashemi F,Scherneck S,Kaiser D,Kluge R,Neschen S,Joost HG,Schürmann A

    更新日期:2011-03-01 00:00:00

  • Skeletal muscle-specific Cre recombinase expression, controlled by the human α-skeletal actin promoter, improves glucose tolerance in mice fed a high-fat diet.

    abstract:AIMS/HYPOTHESIS:Cre-loxP systems are frequently used in mouse genetics as research tools for studying tissue-specific functions of numerous genes/proteins. However, the expression of Cre recombinase in a tissue-specific manner often produces undesirable changes in mouse biology that can confound data interpretation whe...


    pub_type: 杂志文章


    authors: Al Batran R,Gopal K,Martin MD,Ho KL,Almutairi M,Aburasayn H,Eaton F,Campbell JE,Ussher JR

    更新日期:2018-08-01 00:00:00

  • Oesophageal dysmotility, delayed gastric emptying and autonomic neuropathy correlate to disturbed glucose homeostasis.

    abstract:AIMS/HYPOTHESIS:Among diabetic patients, glucose homeostasis may be affected by abnormal gastrointestinal motility and autonomic neuropathy. This study analysed whether oesophageal dysmotility, delayed gastric emptying or autonomic neuropathy affect glucose homeostasis. MATERIALS AND METHODS:Oesophageal manometry and ...


    pub_type: 杂志文章


    authors: Ohlsson B,Melander O,Thorsson O,Olsson R,Ekberg O,Sundkvist G

    更新日期:2006-09-01 00:00:00

  • Dynorphin A, kappa opioid receptors and the antinociceptive efficacy of asimadoline in streptozotocin-induced diabetic rats.

    abstract:AIMS/HYPOTHESIS:We investigated spinal and peripheral kappa opioid systems in diabetic rats. MATERIALS AND METHODS:Dynorphin A, N-methyl-D-aspartate (NMDA) and kappa opioid receptor (KOR) were measured in spinal cord, dorsal root ganglia, peripheral nerves and foot skin of control and streptozotocin-induced diabetic r...


    pub_type: 杂志文章


    authors: Jolivalt CG,Jiang Y,Freshwater JD,Bartoszyk GD,Calcutt NA

    更新日期:2006-11-01 00:00:00

  • Effect of age on the association of non-high-density-lipoprotein cholesterol and apolipoprotein B with cardiovascular mortality in a Mediterranean population with type 2 diabetes: the Casale Monferrato study.

    abstract:AIMS/HYPOTHESIS:Measurement of plasma apolipoprotein (Apo) B may improve prediction of cardiovascular risk, as it provides a measure of the total number of atherogenic particles. The aim of this population-based study was to compare the association of non-HDL-cholesterol, ApoB and the ApoB:ApoA-I ratio with cardiovascu...


    pub_type: 杂志文章,多中心研究


    authors: Bruno G,Merletti F,Biggeri A,Bargero G,Prina-Cerai S,Pagano G,Cavallo-Perin P

    更新日期:2006-05-01 00:00:00

  • Pioglitazone-induced increase of insulin sensitivity in the muscles of the obese Zucker fa/fa rat cannot be explained by local adipocyte differentiation.

    abstract::Thiazolidinediones are potent antidiabetic compounds, which act by enhancing peripheral insulin sensitivity. They are also activators of the peroxisome proliferator activated receptor gamma in adipose tissue. Pioglitazone induces in vivo adipocyte differentiation in the obese Zucker fa/fa rat and hence the capacity of...


    pub_type: 杂志文章


    authors: Hallakou S,Foufelle F,Doaré L,Kergoat M,Ferré P

    更新日期:1998-08-01 00:00:00

  • Serum concentrations of the interferon-gamma-inducible chemokine IP-10/CXCL10 are augmented in both newly diagnosed Type I diabetes mellitus patients and subjects at risk of developing the disease.

    abstract:AIMS/HYPOTHESIS:Chemokines are chemotactic cytokines controlling the recruitment of leukocytes from the blood by regulating integrin adhesiveness. It has been shown that the migration of CD4+Th1 and CD4+Th2 cells is governed by specific chemokines. Increasing evidence suggests that the CD4+Th1 cheomoattractant chemokin...


    pub_type: 杂志文章


    authors: Nicoletti F,Conget I,Di Mauro M,Di Marco R,Mazzarino MC,Bendtzen K,Messina A,Gomis R

    更新日期:2002-08-01 00:00:00

  • Oxidative stress induces insulin resistance by activating the nuclear factor-kappa B pathway and disrupting normal subcellular distribution of phosphatidylinositol 3-kinase.

    abstract:AIMS/HYPOTHESIS:Oxidative stress is associated with diabetes, hypertension and atherosclerosis. Insulin resistance is implicated in the development of these disorders. We tested the hypothesis that oxidative stress induces insulin resistance in rats, and endeavoured to identify mechanisms linking the two. METHODS:Buth...


    pub_type: 杂志文章


    authors: Ogihara T,Asano T,Katagiri H,Sakoda H,Anai M,Shojima N,Ono H,Fujishiro M,Kushiyama A,Fukushima Y,Kikuchi M,Noguchi N,Aburatani H,Gotoh Y,Komuro I,Fujita T

    更新日期:2004-05-01 00:00:00

  • Maternal intake of vitamin D during pregnancy and risk of advanced beta cell autoimmunity and type 1 diabetes in offspring.

    abstract:AIMS/HYPOTHESIS:We evaluated the intake of vitamin D by pregnant Finnish women and examined associations between maternal intake of vitamin D and the development of advanced beta cell autoimmunity and type 1 diabetes in their offspring. METHODS:The research was carried out within the Diabetes Prediction and Prevention...


    pub_type: 杂志文章


    authors: Marjamäki L,Niinistö S,Kenward MG,Uusitalo L,Uusitalo U,Ovaskainen ML,Kronberg-Kippilä C,Simell O,Veijola R,Ilonen J,Knip M,Virtanen SM

    更新日期:2010-08-01 00:00:00

  • The clinical and hormonal (C-peptide and glucagon) profile and liability to ketoacidosis during nutritional rehabilitation in Ethiopian patients with malnutrition-related diabetes mellitus.

    abstract::Cases of malnutrition-related diabetes mellitus conforming to the description of the protein deficient pancreatic diabetes type in Ethiopian patients were compared with Type 1 (insulin-dependent) and Type 2 (non-insulin-dependent) diabetic. Fourteen of 39 malnutrition-related diabetes mellitus patients had fat malabso...


    pub_type: 杂志文章


    authors: Abdulkadir J,Mengesha B,Welde Gebriel Z,Keen H,Worku Y,Gebre P,Bekele A,Urga K,Taddesse AS

    更新日期:1990-04-01 00:00:00

  • Cloning and quantitative determination of the human Ca2+/calmodulin-dependent protein kinase II (CaMK II) isoforms in human beta cells.

    abstract:AIMS/HYPOTHESIS:The Ca2+/calmodulin-dependent protein kinase II (CaMK II) is highly expressed in pancreatic islets and associated with insulin secretion vesicles. The suppression of CaMK II disturbs insulin secretion and insulin gene expression. There are four isoforms of CaMK II, alpha to delta, that are expressed fro...


    pub_type: 杂志文章


    authors: Rochlitz H,Voigt A,Lankat-Buttgereit B,Göke B,Heimberg H,Nauck MA,Schiemann U,Schatz H,Pfeiffer AF

    更新日期:2000-04-01 00:00:00

  • The effects of glucose-induced oxidative stress on growth and extracellular matrix gene expression of vascular smooth muscle cells.

    abstract::Vascular smooth muscle cell (VSMC) dysfunction plays a role in diabetic macrovasculopathy and this may include abnormalities in growth characteristics and the extracellular matrix. As the actual mechanisms by which glucose induces VSMC dysfunction remain unclear, the aim of this study was to assess the potential role ...


    pub_type: 杂志文章


    authors: Sharpe PC,Yue KK,Catherwood MA,McMaster D,Trimble ER

    更新日期:1998-10-01 00:00:00

  • B-type natriuretic peptide (BNP) affects the initial response to intravenous glucose: a randomised placebo-controlled cross-over study in healthy men.

    abstract:AIMS/HYPOTHESIS:B-type natriuretic peptide (BNP) is a hormone released from cardiomyocytes in response to cell stretching and elevated in heart failure. Recent observations indicate a distinct connection between chronic heart failure and diabetes mellitus. This study investigated the role of BNP on glucose metabolism. ...


    pub_type: 杂志文章,随机对照试验


    authors: Heinisch BB,Vila G,Resl M,Riedl M,Dieplinger B,Mueller T,Luger A,Pacini G,Clodi M

    更新日期:2012-05-01 00:00:00

  • Reduced size at birth and persisting reductions in adiposity in recent, compared with earlier, cohorts of infants born to mothers with gestational diabetes mellitus.

    abstract:AIMS/HYPOTHESIS:This study aimed to explore the infancy growth trajectories of 'recent' and 'earlier' offspring of mothers with gestational diabetes mellitus (OGDM), each compared with the same control infants, and investigate whether 'recent' OGDM still exhibit a classical phenotype, with macrosomia and increased adip...


    pub_type: 杂志文章


    authors: Prentice PM,Olga L,Petry CJ,Simmons D,Murphy HR,Hughes IA,Acerini CL,Ong KK,Dunger DB

    更新日期:2019-11-01 00:00:00

  • Insulin autoantibodies and high titre islet cell antibodies are preferentially associated with the HLA DQA1*0301-DQB1*0302 haplotype at clinical type 1 (insulin-dependent) diabetes mellitus before age 10 years, but not at onset between age 10 and 40 years

    abstract::Demographic and biological data were collected from all Caucasian Type 1 diabetic patients (n = 279) who were recruited at clinical onset by the Belgian Diabetes Registry over 34 months. The male/female ratio was significantly higher for onset between age 20 and 40 years (2.4) than before age 20 years (1.0); no age-or...


    pub_type: 杂志文章


    authors: Vandewalle CL,Decraene T,Schuit FC,De Leeuw IH,Pipeleers DG,Gorus FK

    更新日期:1993-11-01 00:00:00

  • Glycated albumin but not HbA1c reflects glycaemic control in patients with neonatal diabetes mellitus.

    abstract:AIMS/HYPOTHESIS:It is difficult to use HbA(1c) as an indicator of glycaemic control in patients with neonatal diabetes mellitus (NDM) because of high levels of fetal haemoglobin (HbF) remaining in the blood. In this study, glycated albumin (GA), which is not affected by HbF, and HbA(1c) were compared to evaluate whethe...


    pub_type: 杂志文章


    authors: Suzuki S,Koga M,Amamiya S,Nakao A,Wada K,Okuhara K,Hayano S,Sarhat AR,Takahashi H,Matsuo K,Tanahashi Y,Fujieda K

    更新日期:2011-09-01 00:00:00