Digital gene expression analysis of gastrointestinal helminth resistance in Scottish blackface lambs.


:Digital gene expression (DGE) analysis offers a route to gene discovery which by-passes the need to develop bespoke arrays for nonmodel species, and is therefore a potentially valuable tool for molecular ecologists. Scottish blackface sheep, which vary in resistance to the common abomasal parasitic nematode Teladorsagia circumcincta, were trickle-infected with L3 larvae over 3 months to mimic the natural progression of infection. DGE was performed on abomasal lymph node tissue after the resolution of infection in resistant animals. Susceptible (low resistance) animals showed a large number of differentially expressed genes associated with inflammation and cell activation, but generally few differentially regulated genes in either the susceptible or the resistant group were directly involved in the adaptive immune function. Our results are consistent with the hypothesis that both resistance and susceptibility are active responses to infection and that susceptibility is associated with dysfunction in T cell differentiation and regulation.


Mol Ecol


Molecular ecology


Pemberton JM,Beraldi D,Craig BH,Hopkins J




Has Abstract


2011-03-01 00:00:00












  • Conservation of sex chromosomes in lacertid lizards.

    abstract::Sex chromosomes are believed to be stable in endotherms, but young and evolutionary unstable in most ectothermic vertebrates. Within lacertids, the widely radiated lizard group, sex chromosomes have been reported to vary in morphology and heterochromatinization, which may suggest turnovers during the evolution of the ...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Rovatsos M,Vukić J,Altmanová M,Johnson Pokorná M,Moravec J,Kratochvíl L

    更新日期:2016-07-01 00:00:00

  • Prevalence of interspecific hybrids amongst asexual fungal endophytes of grasses.

    abstract::Epichloë endophytes are fungal symbionts of grasses that span a continuum including asexual mutualists that are vertically transmitted, obligately sexual pathogens that are horizontally transmitted, and mixed-strategy symbionts with both mutualistic and pathogenic capabilities. Here we show that processes of genome ev...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Moon CD,Craven KD,Leuchtmann A,Clement SL,Schardl CL

    更新日期:2004-06-01 00:00:00

  • Evolutionary history of a dispersal-associated locus across sympatric and allopatric divergent populations of a wing-polymorphic beetle across Atlantic Europe.

    abstract::Studying the evolutionary history of trait divergence, in particular those related to dispersal capacity, is of major interest for the process of local adaptation and metapopulation dynamics. Here, we reconstruct the evolution of different alleles at the nuclear-encoded mitochondrial NADP(+)-dependent isocitrate dehyd...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Van Belleghem SM,Roelofs D,Hendrickx F

    更新日期:2015-02-01 00:00:00

  • Molecular evidence of the survival of subterranean amphipods (Arthropoda) during Ice Age underneath glaciers in Iceland.

    abstract::A Two endemic groundwater arthropod crustacean species, Crangonyx islandicus and Crymostygius thingvallensis, were recently discovered on the mid-Atlantic volcanic island of Iceland. The extent of morphological differences from closest relatives, endemism, along with the geographic isolation of Iceland and its complet...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Kornobis E,Pálsson S,Kristjánsson BK,Svavarsson J

    更新日期:2010-06-01 00:00:00

  • Living the high life: Could gut microbiota matter for adaptation to high altitude?

    abstract::At high altitude, the reduced availability of thermal energy and oxygen poses major challenges to organisms. Different species or populations have evolved similar solutions to these challenges, such as blood flow regulation in animals (Bouverot, 1985). Previous studies investigating such convergent adaptations have pr...

    journal_title:Molecular ecology

    pub_type: 评论,新闻


    authors: Mazel F

    更新日期:2019-05-01 00:00:00

  • Effects of population succession on demographic and genetic processes: predictions and tests in the daylily Hemerocallis thunbergii (Liliaceae).

    abstract::Spatial genetic structure within plant populations is influenced by variation in demographic processes through space and time, including a population's successional status. To determine how demographic structure and fine-scale genetic structure (FSGS) change with stages in a population's successional history, we studi...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Chung MY,Nason JD,Chung MG

    更新日期:2007-07-01 00:00:00

  • Unusual mitochondrial DNA polymorphism in two local populations of blue tit Parus caeruleus.

    abstract::Mitochondrial DNA (mtDNA) from 25 blue tits Parus caeruleus sampled from two populations of the Grenoble region (France) was assayed for polymorphism with 17 restriction endonucleases. Nine genotypes were found. Several mtDNA genotypes were also analysed by amplification via the polymerase chain reaction (PCR) and dir...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Taberlet P,Meyer A,Bouvet J

    更新日期:1992-05-01 00:00:00

  • Selection at behavioural, developmental and metabolic genes is associated with the northward expansion of a successful tropical colonizer.

    abstract::What makes a species able to colonize novel environments? This question is key to understand the dynamics of adaptive radiations and ecological niche shifts, but the mechanisms that underlie expansion into novel habitats remain poorly understood at a genomic scale. Lizards from the genus Anolis are typically tropical,...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Bourgeois Y,Boissinot S

    更新日期:2019-08-01 00:00:00

  • Thermal adaptation in the fungal pathogen Mycosphaerella graminicola.

    abstract::Genetic differentiation in thermal adaptation can result from a trade-off between the performance of organisms across different temperatures or from the accumulation of deleterious mutations. In this experiment, we assayed thermal sensitivity of 138 genetically distinct Mycosphaerella graminicola isolates sampled from...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Zhan J,McDonald BA

    更新日期:2011-04-01 00:00:00

  • Ecological patterns and genetic analysis of post-dispersal seed predation in sunflower (Helianthus annuus) crop-wild hybrids.

    abstract::Crop-wild hybridization has been documented in many cultivated species, but the ecological and genetic factors that influence the likelihood or rate that cultivar alleles will introgress into wild populations are poorly understood. Seed predation is one factor that could mitigate the spread of otherwise advantageous c...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Dechaine JM,Burger JC,Burke JM

    更新日期:2010-08-01 00:00:00

  • Pleistocene refugia and polytopic replacement of diploids by tetraploids in the Patagonian and Subantarctic plant Hypochaeris incana (Asteraceae, Cichorieae).

    abstract::We report the phylogeographic pattern of the Patagonian and Subantarctic plant Hypochaeris incana endemic to southeastern South America. We applied amplified fragment length polymorphism (AFLP) and chloroplast DNA (cpDNA) analysis to 28 and 32 populations, respectively, throughout its distributional range and assessed...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Tremetsberger K,Urtubey E,Terrab A,Baeza CM,Ortiz MA,Talavera M,König C,Temsch EM,Kohl G,Talavera S,Stuessy TF

    更新日期:2009-09-01 00:00:00

  • Prey choice by carabid beetles feeding on an earthworm community analysed using species- and lineage-specific PCR primers.

    abstract::The carabid beetle Pterostichus melanarius is a major natural enemy of pests, such as aphids and slugs in agricultural systems. Earthworms are a dominant non-pest component of the diet of P. melanarius which help sustain the beetles during periods when the pest population is low or absent. In this study we wanted to t...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: King RA,Vaughan IP,Bell JR,Bohan DA,Symondson WO

    更新日期:2010-04-01 00:00:00

  • Genetic diversity and Wolbachia infection of the Drosophila parasitoid Leptopilina clavipes in western Europe.

    abstract::Wolbachia are maternally transmitted bacteria that alter their arthropod hosts' reproduction in various ways, including parthenogenesis induction (PI). Wolbachia-induced parthenogenesis can have drastic effects on the genetic structure of its host because it potentially reduces populations to clones without genetic ex...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Pannebakker BA,Zwaan BJ,Beukeboom LW,Van Alphen JJ

    更新日期:2004-05-01 00:00:00

  • Leveraging eDNA to expand the study of hybrid zones.

    abstract::Hybrid zones are important windows into ecological and evolutionary processes. Our understanding of the significance and prevalence of hybridization in nature has expanded with the generation and analysis of genome-spanning data sets. That said, most hybridization research still has restricted temporal and spatial res...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Stewart KA,Taylor SA

    更新日期:2020-08-01 00:00:00

  • A genomic map of climate adaptation in Mediterranean cattle breeds.

    abstract::Domestic species such as cattle (Bos taurus taurus and B. t. indicus) represent attractive biological models to characterize the genetic basis of short-term evolutionary response to climate pressure induced by their post-domestication history. Here, using newly generated dense SNP genotyping data, we assessed the stru...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Flori L,Moazami-Goudarzi K,Alary V,Araba A,Boujenane I,Boushaba N,Casabianca F,Casu S,Ciampolini R,Coeur D'Acier A,Coquelle C,Delgado JV,El-Beltagi A,Hadjipavlou G,Jousselin E,Landi V,Lauvie A,Lecomte P,Ligda C,Mari

    更新日期:2019-03-01 00:00:00

  • Gene expression patterns underlying parasite-induced alterations in host behaviour and life history.

    abstract::Many parasites manipulate their hosts' phenotype. In particular, parasites with complex life cycles take control of their intermediate hosts' behaviour and life history to increase transmission to their definitive host. The proximate mechanisms underlying these parasite-induced alterations are poorly understood. The c...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Feldmeyer B,Mazur J,Beros S,Lerp H,Binder H,Foitzik S

    更新日期:2016-01-01 00:00:00

  • Hybridization and the porous genome: patterns of isolation and introgression in manakins.

    abstract::How does one species become two? What is the relative importance of selection vs. drift? How many genes are involved, what are their effect sizes and where are they located in the genome? The answers to these questions will inevitably vary from one species to another, but by addressing these questions in a diverse arr...

    journal_title:Molecular ecology

    pub_type: 评论,杂志文章


    authors: Yeaman S

    更新日期:2013-06-01 00:00:00

  • Molecular and quantitative trait variation across the native range of the invasive species Hypericum canariense: evidence for ancient patterns of colonization via pre-adaptation?

    abstract::To understand the success of invasive species, it is important to know whether colonization events are facilitated by adaptive evolution or are limited to sites where a species is pre-adapted to thrive. Studies of the ancient colonization patterns of an invader in its native range provide an opportunity to examine its...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Dlugosch KM,Parker IM

    更新日期:2007-10-01 00:00:00

  • A universal molecular method for identifying underground plant parts to species.

    abstract::As part of a large project to determine rooting depth and resource uptake on the Edwards Plateau of central Texas, we developed a DNA-based technique that allows the below-ground parts of all plants to be identified to the level of genus and usually to species. Identification is achieved by comparing DNA sequences of ...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Linder CR,Moore LA,Jackson RB

    更新日期:2000-10-01 00:00:00

  • Obligate biotrophic pathogens of the genus Albugo are widespread as asymptomatic endophytes in natural populations of Brassicaceae.

    abstract::Mutualistic interactions of plants with true fungi are a well-known and widespread phenomenon, which includes mycorrhiza and non-mycorrhizal endophytes like species of Epichloë. Despite the fact that these organisms intrude into plants, neither strong defence reactions nor the onset of symptoms of disease can be obser...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Ploch S,Thines M

    更新日期:2011-09-01 00:00:00

  • Genetic diversity in water frog hybrids (Pelophylax esculentus) varies with population structure and geographic location.

    abstract::Pelophylax esculentus is a hybridogenetic frog originating from matings between P. ridibundus (RR) and P. lessonae (LL). Typically, diploid hybrids (LR) live in sympatry with one of their parental species, upon which they depend for successful reproduction. In parts of their range, however, pure hybrid populations can...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Arioli M,Jakob C,Reyer HU

    更新日期:2010-05-01 00:00:00

  • Bottlenecks, drift and differentiation: the population structure and demographic history of sika deer (Cervus nippon) in the Japanese archipelago.

    abstract::We assessed genetic differentiation and diversity in 14 populations of sika deer (Cervus nippon) from Japan and four populations of sika deer introduced to the UK, using nine microsatellite loci. We observed extreme levels of differentiation and significant differences in diversity between populations. Our results do ...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Goodman SJ,Tamate HB,Wilson R,Nagata J,Tatsuzawa S,Swanson GM,Pemberton JM,McCullough DR

    更新日期:2001-06-01 00:00:00

  • Multilocus phylogeography of the European ground squirrel: cryptic interglacial refugia of continental climate in Europe.

    abstract::The theory of classical and cryptic Pleistocene refugia is based mainly on historical changes in temperature, and the refugia are usually defined within a latitudinal gradient. However, the gradient of oceanic-continental climate (i.e. longitudinal) was also significantly variable during glacial cycles with important ...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Říčanová Š,Koshev Y,Říčan O,Ćosić N,Ćirović D,Sedláček F,Bryja J

    更新日期:2013-08-01 00:00:00

  • Rapid evolution and selection inferred from the transcriptomes of sympatric crater lake cichlid fishes.

    abstract::Crater lakes provide a natural laboratory to study speciation of cichlid fishes by ecological divergence. Up to now, there has been a dearth of transcriptomic and genomic information that would aid in understanding the molecular basis of the phenotypic differentiation between young species. We used next-generation seq...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Elmer KR,Fan S,Gunter HM,Jones JC,Boekhoff S,Kuraku S,Meyer A

    更新日期:2010-03-01 00:00:00

  • Multicollinearity in spatial genetics: separating the wheat from the chaff using commonality analyses.

    abstract::Direct gradient analyses in spatial genetics provide unique opportunities to describe the inherent complexity of genetic variation in wildlife species and are the object of many methodological developments. However, multicollinearity among explanatory variables is a systemic issue in multivariate regression analyses a...

    journal_title:Molecular ecology

    pub_type: 杂志文章,评审


    authors: Prunier JG,Colyn M,Legendre X,Nimon KF,Flamand MC

    更新日期:2015-01-01 00:00:00

  • Evolution of putative barrier loci at an intermediate stage of speciation with gene flow in campions (Silene).

    abstract::Understanding the origin of new species is a central goal in evolutionary biology. Diverging lineages often evolve highly heterogeneous patterns of genetic differentiation; however, the underlying mechanisms are not well understood. We investigated evolutionary processes governing genetic differentiation between the h...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Liu X,Glémin S,Karrenberg S

    更新日期:2020-09-01 00:00:00

  • Patterns of DNA sequence diversity and genetic structure after a range expansion: lessons from the infinite-island model.

    abstract::It has been long recognized that population demographic expansions lead to distinctive features in the molecular diversity of populations. However, recent simulation results have suggested that a distinction could be made between a pure demographic expansion in an unsubdivided population, and a range expansion in a su...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Excoffier L

    更新日期:2004-04-01 00:00:00

  • Spatial scale affects landscape genetic analysis of a wetland grasshopper.

    abstract::Most landscape genetic studies assess the impact of landscape elements on species' dispersal and gene flow. Many of these studies perform their analysis on all possible population pairs in a study area and do not explicitly consider the effects of spatial scale and population network topology on their results. Here, w...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Keller D,Holderegger R,van Strien MJ

    更新日期:2013-05-01 00:00:00

  • Genetic patterns across an invasion's history: a test of change versus stasis for the Eurasian round goby in North America.

    abstract::Biological invasions comprise accidental evolutionary experiments, whose genetic compositions underlie relative success, spread and persistence in new habitats. However, little is known about whether, or how, their population genetic patterns change temporally and/or spatially across the invasion's history. Theory pre...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Snyder MR,Stepien CA

    更新日期:2017-02-01 00:00:00

  • Intestinal microbiota in fishes: what's known and what's not.

    abstract::High-throughput sequencing approaches have enabled characterizations of the community composition of numerous gut microbial communities, which in turn has enhanced interest in their diversity and functional relationships in different groups of vertebrates. Although fishes represent the greatest taxonomic and ecologica...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Clements KD,Angert ER,Montgomery WL,Choat JH

    更新日期:2014-04-01 00:00:00