Digital gene expression analysis of gastrointestinal helminth resistance in Scottish blackface lambs.


:Digital gene expression (DGE) analysis offers a route to gene discovery which by-passes the need to develop bespoke arrays for nonmodel species, and is therefore a potentially valuable tool for molecular ecologists. Scottish blackface sheep, which vary in resistance to the common abomasal parasitic nematode Teladorsagia circumcincta, were trickle-infected with L3 larvae over 3 months to mimic the natural progression of infection. DGE was performed on abomasal lymph node tissue after the resolution of infection in resistant animals. Susceptible (low resistance) animals showed a large number of differentially expressed genes associated with inflammation and cell activation, but generally few differentially regulated genes in either the susceptible or the resistant group were directly involved in the adaptive immune function. Our results are consistent with the hypothesis that both resistance and susceptibility are active responses to infection and that susceptibility is associated with dysfunction in T cell differentiation and regulation.


Mol Ecol


Molecular ecology


Pemberton JM,Beraldi D,Craig BH,Hopkins J




Has Abstract


2011-03-01 00:00:00












  • Patterns and rates of nucleotide substitution, insertion and deletion in the endosymbiont of ants Blochmannia floridanus.

    abstract::Genome reduction is a general process that has been studied in numerous symbiotic bacteria associated with insects. We investigated the last stages of genome degradation in Blochmannia floridanus, a mutualistic bacterial endosymbiont of the ant Camponotus floridanus. We determined the tempo (rates of insertion and del...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Gómez-Valero L,Latorre A,Gil R,Gadau J,Feldhaar H,Silva FJ

    更新日期:2008-10-01 00:00:00

  • Evolutionary genetics of a defensive facultative symbiont of insects: exchange of toxin-encoding bacteriophage.

    abstract::The facultative endosymbiont of aphids, Hamiltonella defensa, kills parasitoid wasp larvae, allowing aphid hosts to survive and reproduce. This protection may depend on toxins that are encoded by the genomes of H. defensa and of its bacteriophage (APSE). Strains of H. defensa vary in degree of protection conferred upo...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Degnan PH,Moran NA

    更新日期:2008-02-01 00:00:00

  • All roads lead to weediness: Patterns of genomic divergence reveal extensive recurrent weedy rice origins from South Asian Oryza.

    abstract::Weedy rice (Oryza spp.), a weedy relative of cultivated rice (O. sativa), infests and persists in cultivated rice fields worldwide. Many weedy rice populations have evolved similar adaptive traits, considered part of the 'agricultural weed syndrome', making this an ideal model to study the genetic basis of parallel ev...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Huang Z,Young ND,Reagon M,Hyma KE,Olsen KM,Jia Y,Caicedo AL

    更新日期:2017-06-01 00:00:00

  • Allopatric speciation despite historical gene flow: Divergence and hybridization in Carex furva and C. lucennoiberica (Cyperaceae) inferred from plastid and nuclear RAD-seq data.

    abstract::Gene flow among incipient species can act as a creative or destructive force in the speciation process, generating variation on which natural selection can act while, potentially, undermining population divergence. The flowering plant genus Carex exhibits a rapid and relatively recent radiation with many species limit...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Maguilla E,Escudero M,Hipp AL,Luceño M

    更新日期:2017-10-01 00:00:00

  • Genetic variability and phylogeography of the invasive zebra mussel, Dreissena polymorpha (Pallas).

    abstract::The zebra mussel, Dreissena polymorpha (Pallas), a bivalve species originally native to the Black and Caspian seas, has invaded Ireland in the last decade. Five microsatellite loci were used to investigate genetic diversity and population structure in 10 populations across Europe (Ireland, UK, the Netherlands and Roma...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Astanei I,Gosling E,Wilson J,Powell E

    更新日期:2005-05-01 00:00:00

  • Microsatellite analysis of the phylogeography, Pleistocene history and secondary contact hypotheses for the killifish, Fundulus heteroclitus.

    abstract::The mummichog, Fundulus heteroclitus, exhibits extensive latitudinal clinal variation in a number of physiological and biochemical traits, coupled with phylogeographical patterns at mitochondrial and nuclear DNA loci that suggest a complicated history of spatially variable selection and secondary intergradation. This ...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Adams SM,Lindmeier JB,Duvernell DD

    更新日期:2006-04-01 00:00:00

  • Phylogeography of the sergeants Abudefduf sexfasciatus and A. vaigiensis reveals complex introgression patterns between two widespread and sympatric Indo-West Pacific reef fishes.

    abstract::On evolutionary timescales, sea level oscillations lead to recurrent spatio-temporal variation in species distribution and population connectivity. In this situation, applying classical concepts of biogeography is challenging yet necessary to understand the mechanisms underlying biodiversity in highly diverse marine e...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Bertrand JAM,Borsa P,Chen WJ

    更新日期:2017-05-01 00:00:00

  • A hybrid zone provides evidence for incipient ecological speciation in Heliconius butterflies.

    abstract::In Heliconius butterflies, it has been proposed that speciation occurs through a combination of divergence in ecological habitat preferences and mimetic colour patterns. Here we test this hypothesis by investigating a parapatric form of the widespread species Heliconius erato. Mendelian (colour patterns) and molecular...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Arias CF,Muñoz AG,Jiggins CD,Mavárez J,Bermingham E,Linares M

    更新日期:2008-11-01 00:00:00

  • Long-term population isolation in the endangered tropical tree species Caesalpinia echinata Lam. revealed by chloroplast microsatellites.

    abstract::Habitat fragmentation represents the single most serious threat to the survival of tropical ecosystems. In formulating strategies to counteract the detrimental effects of fragmentation, knowledge of the levels and patterns of genetic diversity within and between natural populations is vital to the establishment of any...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Lira CF,Cardoso SR,Ferreira PC,Cardoso MA,Provan J

    更新日期:2003-12-01 00:00:00

  • Conservation genomics of Atlantic salmon: variation in gene expression between and within regions of the Bay of Fundy.

    abstract::Due to difficulties in identifying important within-species biodiversity for effective species management and conservation, the use of multiple complementary sources of information is required to identify and assess the designation of conservation units based on the degree of variation among populations within a speci...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Vandersteen Tymchuk W,O'Reilly P,Bittman J,Macdonald D,Schulte P

    更新日期:2010-05-01 00:00:00

  • Dispersal ability and habitat requirements determine landscape-level genetic patterns in desert aquatic insects.

    abstract::Species occupying the same geographic range can exhibit remarkably different population structures across the landscape, ranging from highly diversified to panmictic. Given limitations on collecting population-level data for large numbers of species, ecologists seek to identify proximate organismal traits-such as disp...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Phillipsen IC,Kirk EH,Bogan MT,Mims MC,Olden JD,Lytle DA

    更新日期:2015-01-01 00:00:00

  • Host association drives genetic divergence in the bed bug, Cimex lectularius.

    abstract::Genetic differentiation may exist among sympatric populations of a species due to long-term associations with alternative hosts (i.e. host-associated differentiation). While host-associated differentiation has been documented in several phytophagus insects, there are far fewer cases known in animal parasites. The bed ...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Booth W,Balvín O,Vargo EL,Vilímová J,Schal C

    更新日期:2015-03-01 00:00:00

  • Evolution of the mating system in colonizing plants.

    abstract::Colonization is likely to be more successful for species with an ability to self-fertilize and thus to establish new populations as single individuals. As a result, self-compatibility should be common among colonizing species. This idea, labelled 'Baker's law', has been influential in discussions of sexual-system and ...

    journal_title:Molecular ecology

    pub_type: 杂志文章,评审


    authors: Pannell JR

    更新日期:2015-05-01 00:00:00

  • High specificity generally characterizes mycorrhizal association in rare lady's slipper orchids, genus Cypripedium.

    abstract::Lady's slipper orchids (Cypripedium spp.) are rare terrestrial plants that grow throughout the temperate Northern Hemisphere. Like all orchids, they require mycorrhizal fungi for germination and seedling nutrition. The nutritional relationships of adult Cypripedium mycorrhizae are unclear; however, Cypripedium distrib...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Shefferson RP,Weiss M,Kull T,Taylor DL

    更新日期:2005-02-01 00:00:00

  • How the truffle got its mate: insights from genetic structure in spontaneous and planted Mediterranean populations of Tuber melanosporum.

    abstract::The life cycles and dispersal of edible fungi are still poorly known, thus limiting our understanding of their evolution and domestication. The prized Tuber melanosporum produces fruitbodies (fleshy organs where meiospores mature) gathered in natural, spontaneously inoculated forests or harvested in plantations of nur...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Taschen E,Rousset F,Sauve M,Benoit L,Dubois MP,Richard F,Selosse MA

    更新日期:2016-11-01 00:00:00

  • Comparison of gene expression in segregating families identifies genes and genomic regions involved in a novel adaptation, zinc hyperaccumulation.

    abstract::One of the challenges of comparative genomics is to identify specific genetic changes associated with the evolution of a novel adaptation or trait. We need to be able to disassociate the genes involved with a particular character from all the other genetic changes that take place as lineages diverge. Here we show that...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Filatov V,Dowdle J,Smirnoff N,Ford-Lloyd B,Newbury HJ,Macnair MR

    更新日期:2006-09-01 00:00:00

  • Evidence of inbreeding depression but not inbreeding avoidance in a natural house sparrow population.

    abstract::Inbreeding is common in small and threatened populations and often has a negative effect on individual fitness and genetic diversity. Thus, inbreeding can be an important factor affecting the persistence of small populations. In this study, we investigated the effects of inbreeding on fitness in a small, wild populati...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Billing AM,Lee AM,Skjelseth S,Borg AA,Hale MC,Slate J,Pärn H,Ringsby TH,Saether BE,Jensen H

    更新日期:2012-03-01 00:00:00

  • Using long-term experimental evolution to uncover the patterns and determinants of molecular evolution of an Escherichia coli natural isolate in the streptomycin-treated mouse gut.

    abstract::Although microbial ecology of the gut is now a major focus of interest, little is known about the molecular determinants of microbial adaptation in the gut. Experimental evolution coupled with whole-genome sequencing can provide insights of the adaptive process. In vitro experiments have revealed some conserved patter...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Lescat M,Launay A,Ghalayini M,Magnan M,Glodt J,Pintard C,Dion S,Denamur E,Tenaillon O

    更新日期:2017-04-01 00:00:00

  • Multicollinearity in spatial genetics: separating the wheat from the chaff using commonality analyses.

    abstract::Direct gradient analyses in spatial genetics provide unique opportunities to describe the inherent complexity of genetic variation in wildlife species and are the object of many methodological developments. However, multicollinearity among explanatory variables is a systemic issue in multivariate regression analyses a...

    journal_title:Molecular ecology

    pub_type: 杂志文章,评审


    authors: Prunier JG,Colyn M,Legendre X,Nimon KF,Flamand MC

    更新日期:2015-01-01 00:00:00

  • Isolation by distance among California sea lion populations in Mexico: redefining management stocks.

    abstract::Understanding the spatial structure of a population is critical for effective assessment and management. However, direct observation of spatial dynamics is generally difficult, particularly for marine mammals. California sea lions (Zalophus californianus) are polygynous pinnipeds distributed along the Pacific coast of...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: González-Suárez M,Flatz R,Aurioles-Gamboa D,Hedrick PW,Gerber LR

    更新日期:2009-03-01 00:00:00

  • Genetic diversity and Wolbachia infection of the Drosophila parasitoid Leptopilina clavipes in western Europe.

    abstract::Wolbachia are maternally transmitted bacteria that alter their arthropod hosts' reproduction in various ways, including parthenogenesis induction (PI). Wolbachia-induced parthenogenesis can have drastic effects on the genetic structure of its host because it potentially reduces populations to clones without genetic ex...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Pannebakker BA,Zwaan BJ,Beukeboom LW,Van Alphen JJ

    更新日期:2004-05-01 00:00:00

  • Biogeography, habitat transitions and hybridization in a radiation of South American silverside fishes revealed by mitochondrial and genomic RAD data.

    abstract::Rivers and lake systems in the southern cone of South America have been widely influenced by historical glaciations, carrying important implications for the evolution of aquatic organisms, including prompting transitions between marine and freshwater habitats and by triggering hybridization among incipient species via...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Hughes LC,Cardoso YP,Sommer JA,Cifuentes R,Cuello M,Somoza GM,González-Castro M,Malabarba LR,Cussac V,Habit EM,Betancur-R R,Ortí G

    更新日期:2020-02-01 00:00:00

  • Pleistocene refugia and polytopic replacement of diploids by tetraploids in the Patagonian and Subantarctic plant Hypochaeris incana (Asteraceae, Cichorieae).

    abstract::We report the phylogeographic pattern of the Patagonian and Subantarctic plant Hypochaeris incana endemic to southeastern South America. We applied amplified fragment length polymorphism (AFLP) and chloroplast DNA (cpDNA) analysis to 28 and 32 populations, respectively, throughout its distributional range and assessed...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Tremetsberger K,Urtubey E,Terrab A,Baeza CM,Ortiz MA,Talavera M,König C,Temsch EM,Kohl G,Talavera S,Stuessy TF

    更新日期:2009-09-01 00:00:00

  • Expanding host specificity and pathogen sharing beyond viruses.

    abstract::Most emerging pathogens of humans can infect multiple host species (Woolhouse & Gowtage-Sequeria, 2005). This simple fact has motivated multiple large-scale, comparative analyses of the drivers of pathogen sharing and zoonotic pathogen richness among hosts as well as the factors determining the zoonotic potential of p...

    journal_title:Molecular ecology

    pub_type: 评论,新闻


    authors: Becker DJ,Albery GF

    更新日期:2020-09-01 00:00:00

  • Structure and evolution of the sea star egg receptor for sperm bindin.

    abstract::Selection on coevolving sperm- and egg-recognition molecules is a potent engine of population divergence leading to reproductive isolation and speciation. The study of receptor-ligand pairs can reveal co-evolution of male- and female-expressed genes or differences between their evolution in response to selective facto...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Hart MW

    更新日期:2013-04-01 00:00:00

  • Bacterial diversity in a glacier foreland of the high Arctic.

    abstract::Over the past 100 years, Arctic temperatures have increased at almost twice the global average rate. One consequence is the acceleration of glacier retreat, exposing new habitats that are colonized by microorganisms whose diversity and function are unknown. Here, we characterized bacterial diversity along two approxim...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Schütte UM,Abdo Z,Foster J,Ravel J,Bunge J,Solheim B,Forney LJ

    更新日期:2010-03-01 00:00:00

  • Quantifying dispersal variability among nearshore marine populations.

    abstract::Dispersal drives diverse processes from population persistence to community dynamics. However, the amount of temporal variation in dispersal and its consequences for metapopulation dynamics is largely unknown for organisms with environmentally driven dispersal (e.g., many marine larvae, arthropods and plant seeds). He...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Catalano KA,Dedrick AG,Stuart MR,Puritz JB,Montes HR Jr,Pinsky ML

    更新日期:2020-11-16 00:00:00

  • Contemporary human-altered landscapes and oceanic barriers reduce bumble bee gene flow.

    abstract::Much of the world's terrestrial landscapes are being altered by humans in the form of agriculture, urbanization and pastoral systems, with major implications for biodiversity. Bumble bees are one of the most effective pollinators in both natural and cultivated landscapes, but are often the first to be extirpated in hu...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Jha S

    更新日期:2015-03-01 00:00:00

  • Molecular and quantitative trait variation across the native range of the invasive species Hypericum canariense: evidence for ancient patterns of colonization via pre-adaptation?

    abstract::To understand the success of invasive species, it is important to know whether colonization events are facilitated by adaptive evolution or are limited to sites where a species is pre-adapted to thrive. Studies of the ancient colonization patterns of an invader in its native range provide an opportunity to examine its...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Dlugosch KM,Parker IM

    更新日期:2007-10-01 00:00:00

  • Demographic analyses of marine and terrestrial snakes (Elapidae) using whole genome sequences.

    abstract::The question of whether spatial aspects of evolution differ in marine versus terrestrial realms has endured since Ernst Mayr's 1954 essay on marine speciation. Marine systems are often suggested to support larger and more highly connected populations, but quantitative comparisons with terrestrial systems have been lac...

    journal_title:Molecular ecology

    pub_type: 杂志文章


    authors: Ludington AJ,Sanders KL

    更新日期:2020-11-10 00:00:00