Abstract:
:The immune modulating capacity of vitamin D(3) is well-recognized. Ultra-violet (UV) exposure determines production of vitamin D(3) in vivo and varies through the course of the year, especially in temperate regions. However, it is not known whether the human innate immune response differs due to seasonality. To validate the seasonal effects of vitamin D(3) , the effect of 1,25(OH)(2) D(3) on peripheral blood mononuclear cells (PBMC) cytokine response was first determined in vitro. 1,25(OH)(2) D(3) decreased interleukin (IL)-6 and tumour necrosis factor (TNF)-α release by PBMC stimulated with tripalmitoyl-S-glycerylcysteine (Pam3Cys) or lipopolysaccharide (LPS). Subsequently, ex-vivo stimulation studies were performed in 15 healthy volunteers through the course of the four seasons of the year. PBMC were isolated and stimulated with Toll-like receptor (TLR)-2 and TLR-4 ligands Pam3Cys and LPS, respectively. Circulating concentrations of 25(OH)D(3) and 1,25(OH)(2) D(3) were higher during summer (P<0·05) and a down-regulation of TLR-4-mediated IL-1β, IL-6, TNF-α, interferon (IFN)-γ and IL-10 production in summer was observed compared to winter (P<0·05). The variation in cytokine response upon TLR-2 (Pam3Cys) stimulation was moderate throughout the four seasons. The repressed cytokine production during the summer months could be explained partly by the reduced cell-membrane expression of TLRs. Physiological variation in vitamin D(3) status through the four seasons of the year can lead to alteration in the innate immune responses. Elevated vitamin D(3) level in vivo is associated with down-regulation of cytokine response through diminished surface expression of pattern recognition receptors.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Khoo AL,Chai LY,Koenen HJ,Sweep FC,Joosten I,Netea MG,van der Ven AJdoi
10.1111/j.1365-2249.2010.04315.xsubject
Has Abstractpub_date
2011-04-01 00:00:00pages
72-9issue
1eissn
0009-9104issn
1365-2249journal_volume
164pub_type
杂志文章abstract::Compared to normal children, pre-natally infected infants had significantly elevated IgM concentrations throughout the first year of life and elevated IgA levels for the first 6 months. In contrast, IgG levels dropped significantly below normal at 3 months post-partum. Infants born without overt disease but with eleva...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1981-05-01 00:00:00
abstract::The localization of antigens reacting with parietal cell antibodies found in the sera of some patients with pernicious anaemia was studied by the indirect immunoperoxidase method on viable gastric glands isolated from the mouse or human fundus by collagenase digestion. We observed that only the luminal surface of pari...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1983-08-01 00:00:00
abstract::Interspecies immunization of marmosets with platelets leads to an acute thrombocytopenia which appears to be immunologically mediated. The thrombocytopenia is preceded by the formation of antibodies to the donor platelet antigen and deposition of IgG on the host's platelets. Examination of sera obtained from these ani...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1981-01-01 00:00:00
abstract::Thyroid autoantigens require internalization and processing by antigen-presenting cells to induce immune responses. Besides pinocytosis, antigen uptake can be receptor-mediated. The mannose receptor (ManR) has a cysteine rich domain (CR) and eight carbohydrate recognition domains (CRD) that bind glycosylated proteins....
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2004.02689.x
更新日期:2005-02-01 00:00:00
abstract::Human peripheral blood lymphocytes were examined for rosette formation with autologous erythrocytes. When normal human lymphocytes were stimulated with phorbol myristate acetate (PMA) in the presence of autologous serum, the levels of autorosette forming cells (ARFC) were strongly enhanced. Pre-culture was necessary f...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-09-01 00:00:00
abstract::The aim of the present study was to investigate the prevalence of C4 and C2 deficiencies and to characterize genomic alterations in C4 genes in a large cohort of 125 unselected patients with SLE. We determined the protein concentration and functional activity of C2 and C4, as well as the C4 phenotype. C4 genotyping in...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2001.01438.x
更新日期:2001-01-01 00:00:00
abstract::Human lymphokine-activated killer (LAK) cells developed by an incubation of peripheral mononuclear cells with IL-2 express the membrane-associated lymphotoxin (LT)-related molecule (mLT). By a further cultivation of mLT expressing (mLT-positive) LAK cells for 24 h without IL-2, mLT disappears (mLT-negative LAK cells)....
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1994.tb06245.x
更新日期:1994-04-01 00:00:00
abstract::Common variable immunodeficiency (CVID) is a heterogeneous syndrome characterized by defective immunoglobulin production and high frequency of bacterial infections, autoimmunity and manifestations of chronic inflammation. The homeostatic chemokines CCL19 and CCL21 and their receptor CCR7 are associated with modulation...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2009.04013.x
更新日期:2009-11-01 00:00:00
abstract::Circulating immune complexes have been investigated in jirds (Meriones unguiculatus) infected with the filarial nematode Brugia pahangi. Two-month-old male jirds were inoculated with seventy-five B. pahangi infective larvae into the left groin. At 8 months post-infection, sera of individual animals from a group of sev...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::Mixed leucocyte culture blocking factor activity (MLC-BFA) in the plasma of haemodialysis patients appears as a result of recent blood transfusions and is concentrated in IgG fractions of the blocking serum. Four patients neither developed lymphocytotoxins nor MLC-BFA in spite of having received multiple blood transfu...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1975-05-01 00:00:00
abstract::To determine the role of chemokines in the pathogenesis of systemic sclerosis (SSc), we examined serum levels, spontaneous production by peripheral blood mononuclear cells (PBMC), and histological distribution in the affected skin, of MCP-1, MIP-1alpha and MIP-1beta in SSc patients. Serum levels of these chemokines we...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1999.00929.x
更新日期:1999-07-01 00:00:00
abstract::The specific response of human peripheral blood lymphocyte cultures to TNP-polyacrylamide was suppressed by the addition of concanavalin A (Con A). A dose of Con A (0.5 microgram/ml) could be selected, which induced a reproducible but incomplete suppression independent of the magnitude of the anti-TNP response. Con A-...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1979-10-01 00:00:00
abstract::Serum activity of the adenosine deaminase (ADA) isozyme, ADA2, has been reported to be elevated during various disease states. Macrophages have been suggested as the cellular source of extracellular ADA activity because they are one of the only cell types in which intracellular ADA2 activity has been measured, but ext...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2004.02591.x
更新日期:2004-10-01 00:00:00
abstract::Immunoglobulin G (IgG) in soluble and particulate fractions of discrete tissue samples from multiple sclerosis (MS) brain was analysed. Supernatant IgG/albumin rations and particulate-bound IgG levels were highest in samples dissected from MS plaques and adjacent white matter. Acid extracts of particulate fractions fr...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-04-01 00:00:00
abstract::Patients with common variable immunodeficiency (CVID) have reduced numbers and frequencies of dendritic cells (DCs) in blood, and there is also evidence for defective activation through Toll-like receptors (TLRs). Collectively, these observations may point to a primary defect in the generation of functional DCs. Here,...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/cei.12239
更新日期:2014-03-01 00:00:00
abstract::Aspergillus fumigatus, a ubiquitous fungus, is implicated in the pathogenesis of a number of clinically different allergic diseases in man, including allergic bronchopulmonary aspergillosis. Peptide-based immunotherapy may offer an alternative treatment strategy for the management of allergic disease. The objective of...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2002.01776.x
更新日期:2002-02-01 00:00:00
abstract::An injection of human renal tubular fraction, Fx1A, with Freund's complete adjuvant in rats induces Heymann nephritis (HN) with clinical and morphological features similar to rat Fx1A-induced HN. The present study was undertaken to characterize antibody specificities and the corresponding antigen involved in human Fx1...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract::Human thymoma is a thymic epithelial cell tumour which often contains a large number of immature T cells and is frequently associated with autoimmune diseases. Since thymic epithelial cells play key roles in the development and selection of T cells in the normal thymus, we hypothesized that the neoplastic thymic epith...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.1998.00606.x
更新日期:1998-06-01 00:00:00
abstract::Two-stage stimulation of SK activity of normal human peripheral blood lymphocytes by soluble factors was demonstrated. The first stage was initiated by factors present in supernatant derived from normal B-LCL cultures. Only cell lines which could induce SK activity in culture in an MLR-type reaction had the capacity t...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::Haemorrhage produces alterations in macrophage, T and B cell function. In order to better define the mechanism for the effects of blood loss on immune response, we examined function of and cytokine production by purified T cells, CD4+ and CD8+ subpopulations after blood loss. Whereas T and CD4+ cells from control, unh...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1992.tb05873.x
更新日期:1992-12-01 00:00:00
abstract::Twenty-one patients with primary immunoglobulin deficiency were enrolled in a crossover study to test the efficacy and safety of Alphaglobin in comparison with the licensed preparations Sandoglobulin and Gamimune. There was no statistical difference in these parameters between Alphaglobin and Sandoglobulin/Gamimune. T...
journal_title:Clinical and experimental immunology
pub_type: 临床试验,杂志文章
doi:10.1111/j.1365-2249.1995.tb03465.x
更新日期:1995-01-01 00:00:00
abstract::In this study which included 16 patients with inflammatory or non-inflammatory arthropathies, human granulocyte-macrophage colony-stimulating activity was detected in synovial fluid. This was attributable to the presence of colony-stimulating factor(s) (CSF), as a direct action on human bone marrow progenitor cells wa...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1988-04-01 00:00:00
abstract::The intestinal population of gamma delta T cell receptor (TCR)-bearing cells was characterized with regard to V delta and V gamma subtype expression. For this purpose, we utilized V gene-specific PCR of mRNA prepared from intestinal biopsies. Predominant expression of the V delta 1 subtype was demonstrated in the smal...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1995.tb03472.x
更新日期:1995-01-01 00:00:00
abstract::During blood-stage infection of mice with a lethal variant of Plasmodium yoelii, cells in both spleen and liver became activated to reach a peak at day 5. In mice protected by vaccination, activation was accelerated after infection. The most striking difference observed was in the 10-fold greater yield of infiltrating...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1987-12-01 00:00:00
abstract::The effect of administration of 0-2 mg of epinephrine (Parke-Davis) on circulatory lymphocytes was investigated in fifteen normal subjects. Blood samples were taken prior to, 10 and 20 min after, subcutaneous injections. Thymus-derived (T) cells were assayed by sheep red blood cell rosettes, bone marrow-derived (B) ce...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1976-09-01 00:00:00
abstract::Peripheral blood mononuclear cells (PBMC) of normal human donors are spontaneously cytotoxic for certain tumour-derived and virus-infected target cells. This so-called natural killing (NK) can be augmented by the action of interferons (IFN) and by IFN-inducers. In this study, we have compared both endogenous and augme...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::The in-vivo clearance of Bordetella pertussis infections in murine models in naive mice and animals vaccinated with whole-cell vaccine is considered to be via a Th-1-dependent mechanism in which interleukin-12 (IL)-12 may play a prominent role. It has also been demonstrated clearly that the treatment of animals with m...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.2003.02352.x
更新日期:2004-02-01 00:00:00
abstract::Primary biliary cholangitis (PBC) is a multi-factorial disease caused by the interaction of both genetic predisposition and environmental triggers. Bacterial infection has been investigated most intensively, both epidemiologically and experimentally, as a prime environmental aetiology in PBC. The association of recurr...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章,评审
doi:10.1111/cei.13198
更新日期:2019-01-01 00:00:00
abstract::Data from a previous study suggested that peripheral blood monocytes in patients with rheumatoid arthritis (RA) may be activated. Therefore, in this study we sought further evidence of 'presynovial' activation of monocytes. Our results show that phenotypic changes are demonstrable in peripheral blood monocytes in pati...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1111/j.1365-2249.1995.tb03850.x
更新日期:1995-12-01 00:00:00
abstract::Blood monocytes of patients with thyroid autoimmune disease (TAID) display defects in rearranging their cortical actomyosin cytoskeleton ('polarize') in response to chemoattractants. Such rearrangements also take place after the adherence of monocytes to the extracellular matrix (ECM). It is therefore not surprising t...
journal_title:Clinical and experimental immunology
pub_type: 杂志文章
doi:10.1046/j.1365-2249.2001.01583.x
更新日期:2001-07-01 00:00:00