Abstract:
OBJECTIVE:Dyslipidemia is commonly seen in patients with end-stage renal disease (ESRD). This prospective study investigates whether small-sized high-density lipoprotein (HDL) particles alone or in combination with high sensitivity C-reactive protein (hsCRP) are independent determinants of ESRD mortality. DESIGN AND METHODS:We performed 36 months follow-up study in 122 haemodialysis (HD) patients. HDL size and subclass distribution were determined by gradient gel electrophoresis. Baseline characteristics of the patients were evaluated for the prediction of mortality. RESULTS:Cox regressions analysis showed that patients with small-sized HDL particles had 2.8-fold higher risk of lethal outcome (P<0.05). Concomitant presence of small-sized HDL particles and increased hsCRP concentration were significantly associated with reduced survival rate (HR=3.907; P<0.05). Observed relationships persisted after adjustment for serum lipid and lipoprotein concentrations. CONCLUSIONS:Our results indicate that small-sized HDL particles alone and combined with elevated hsCRP concentrations are independent predictors of reduced survival in HD patients.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Vekic J,Zeljkovic A,Bogavac-Stanojevic N,Jelic-Ivanovic Z,Spasojevic-Kalimanovska V,Simic-Ogrizovic S,Dopsaj V,Spasic Sdoi
10.1016/j.clinbiochem.2011.02.002subject
Has Abstractpub_date
2011-06-01 00:00:00pages
635-41issue
8-9eissn
0009-9120issn
1873-2933pii
S0009-9120(11)00064-6journal_volume
44pub_type
杂志文章abstract::Post-transplant ischemic acute kidney injury (AKI), secondary to ischemia reperfusion injury (IRI), is a major problem influencing on the short and long term graft and patient survival. Many molecular and cellular modifications are observed during IRI, for example, tissue damage result production of reactive oxygen sp...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2018.03.022
更新日期:2018-05-01 00:00:00
abstract::Increased hemoglobin A1 (HbA1) occurs in diabetes mellitus as a direct consequence of hyperglycemia, which causes postsynthetic modification of hemoglobin proportional to blood glucose concentration. Glycosylation of hemoglobin is demonstrable also in vitro, not only by glucose, but with other reducing sugars such as ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(82)90556-2
更新日期:1982-06-01 00:00:00
abstract::The ES 300 system, a fully automated multichannel immunoassay analyzer, was evaluated simultaneously for 9 weeks in four major centers. Precision, accuracy, carryover, comparison to in-house methods, and interferences were assessed for the following 17 tests: T4, T3, FT4, TSH, TBK, TBG, LH, FSH, prolactin, HCG, digoxi...
journal_title:Clinical biochemistry
pub_type: 杂志文章,多中心研究
doi:10.1016/0009-9120(93)90017-z
更新日期:1993-02-01 00:00:00
abstract:OBJECTIVES:CA 15-3 and CEA are considered useful tumor markers in monitoring breast cancer patients. This study was undertaken to specifically evaluate the transient elevations in these markers that are observed during systemic treatment for metastatic disease. This phenomenon has been termed "spiking." DESIGN AND MAT...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(96)00133-6
更新日期:1997-02-01 00:00:00
abstract:BACKGROUND:Therapeutic drug monitoring (TDM) of the widely prescribed antidepressant fluoxetine (FLU) is recommended in certain situations, such as occurrence of toxicity, inadequate response or suspect of poor adherence. Dried blood spot (DBS) sampling is an increasingly studied alternative for TDM, particularly for o...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2017.10.002
更新日期:2018-02-01 00:00:00
abstract:OBJECTIVES:Accurate measurement of IgG subclass (IgGSc) levels are essential to aid in the diagnosis of disease states such as primary immunodeficiencies. However, there is no single standardisation of nephelometric and turbidimetric assays for these analytes and two reference materials have been utilised. We expand on...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2013.07.014
更新日期:2013-11-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to determine the reference values for serum cystatin C (CysC) with a particular focus on the effect of aging. DESIGN AND METHODS:The study was performed on a consecutive series of subjects (258 men and 396 women). Laboratory parameters and a detailed personal and family medical his...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2006.03.017
更新日期:2006-06-01 00:00:00
abstract::The clinical role of HER-2/neu, a 185 kD epithelial transmembranous protein, has evolved after the approval of the anti-HER-2/neu targeted monoclonal antibody trastuzumab (Herceptin) for the therapy of metastatic breast cancer. The extracellular domain of HER-2/neu undergoes proteolytic cleavage from the full-length p...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/s0009-9120(03)00026-2
更新日期:2003-06-01 00:00:00
abstract::Three commercially available assays (an enzyme-linked immunosorbent assay ELISA, an immunoradiometric assay, IRMA, and a nephelometric assay) for the determination of lipoprotein(a) [Lp(a)] were compared with respect to the dependency of these assays on the various apolipoprotein(a) [apo(a)] isoforms. Although there w...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(94)00052-w
更新日期:1994-12-01 00:00:00
abstract:OBJECTIVE:To evaluate the potential diagnostic value of apolipoprotein E (ApoE) measurements in pediatric patients with invasive bacterial infections. DESIGN AND METHOD:A total of 185 pediatric patients were enrolled in this study, including 94 patients with confirmable infections and 91 patients without confirmable i...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2011.11.009
更新日期:2012-02-01 00:00:00
abstract:BACKGROUND:Vitamin B12 insufficiency/deficiency is common in mixed patient populations. However there is no single marker which can reliably diagnose B12 insufficiency/deficiency. Elevated concentrations of methylmalonic acid (MMA) are considered the most representative marker of metabolic vitamin B12 insufficiency, bu...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2013.08.006
更新日期:2014-01-01 00:00:00
abstract:OBJECTIVE:To evaluate clinical value of oxidized lipoprotein(a) [ox-Lp(a)] levels. DESIGN AND METHODS:Ox-Lp(a) were measured by 2 ELISAs using antibodies against ox-Lp(a) [ox-Lp(a)1] or oxidized low-density lipoprotein [ox-Lp(a)2], and studied in 161 acute coronary syndromes (ACS) patients, 114 stable coronary artery ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2009.12.017
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVES:To monitor increases or decreases in cardiovascular disease (CVD)-related proteins that will be released from the deposits or plaque on the inner wall of blood vessels. DESIGN AND METHODS:Protein profiles of sera from healthy subjects and CVD patients were determined via 2-DE. Differentially expressed spots...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.09.027
更新日期:2011-02-01 00:00:00
abstract:OBJECTIVES:Measurement of serotonin concentrations in blood is considered to be important for the clinical studies of diseases involving vascular endothelial injury. We have developed a high-performance liquid chromatographic (HPLC) method for measurement of serotonin level in platelet-poor plasma (PPP). METHODS:Venou...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2003.11.009
更新日期:2004-03-01 00:00:00
abstract:OBJECTIVES:Behçet's disease is a multisystem disorder characterized by a chronic inflammation including acute attacks and remission periods. Decreased enzyme activity of the antioxidant system and increased levels of free radicals may have important roles in the damage of tissues observed in the disease period. In addi...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(02)00290-4
更新日期:2002-05-01 00:00:00
abstract::The bromocresol purple (BCP) albumin method on the DuPont aca was evaluated by determining the accuracy and precision of the method. A split sample comparison was performed against the electroimmunoassay (EIA) method as well as the bromocresol green (BCG) method on the DuPont aca and the Technicon SMA 12/60. The BCP m...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(83)90490-3
更新日期:1983-04-01 00:00:00
abstract:OBJECTIVE:To assess the generation of reactive oxygen species (ROS) and the involvement of the main antioxidant pathways in low/intermediate-1-risk myelodysplastic syndromes (MDS) with iron overload (IOL). METHODS:We examined the levels of superoxide anion (O2-), hydrogen peroxide (H2O2), antioxidants (glutathione, GS...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2017.05.018
更新日期:2017-11-01 00:00:00
abstract:INTRODUCTION:The USA hCG Reference Service has been dealing with cases of persistent low levels of hCG and gestational trophoblastic diseases for 10years. Here we present the complete experience. METHODS:Total hCG in serum and urine was measured using the Siemen's Immulite 1000 assay. Hyperglycosylated hCG, nicked hCG...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2010.05.006
更新日期:2010-08-01 00:00:00
abstract::Plasma, erythrocyte, and leucocyte magnesium (Mg) concentration, as well as urinary Mg, calcium (Ca), and phosphorus (P) excretion were determined in male subjects with elevated diastolic blood pressure (DBP) > 90 mmHg. These parameters were compared to those in normotensive age-matched (DBP < 85 mmHg) and drug-treate...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(93)90027-4
更新日期:1993-06-01 00:00:00
abstract::Inherited and acquired changes in pre-mRNA splicing have been documented to play a significant role in human disease development and many cancer-associated genes are regulated by alternative splicing. Loss of fidelity, variation of the splicing process, even controlled switching to specific splicing alternatives may o...
journal_title:Clinical biochemistry
pub_type: 杂志文章,评审
doi:10.1016/j.clinbiochem.2004.05.015
更新日期:2004-07-01 00:00:00
abstract:OBJECTIVES:We describe a quality assurance procedure to maximize the value of oligonucleotide microarray expression profiles. DESIGN, METHODS, AND RESULTS:Background microarray noise was 82.2+/-54.5 and 51.8+/-12.4 units, respectively, before and after enacting the program (P<0.0001). We also noted improved concordanc...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2005.04.010
更新日期:2005-07-01 00:00:00
abstract:OBJECTIVES:To measure serum cholinesterase (SCHE) with an integration strategy. DESIGN AND METHODS:At 54.0 micromol/L butyrylthiolcholine, SCHE initial rates were calculated with 50.0 micromol/L butyrylthiolcholine and maximal rates via an improved integrated method if substrate consumptions within 5.0 min were over 6...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.11.016
更新日期:2009-06-01 00:00:00
abstract::A method is described for xylose determination on the Ciba-Corning 550 Express that utilizes the slower enzymatic action of glucose dehydrogenase (GDH) on D-xylose, after prior removal of glucose. Glucose oxidase is added to serum or urine and incubated for 120 min at 37 degrees C. After incubation, a perchloric acid ...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/0009-9120(94)90006-x
更新日期:1994-02-01 00:00:00
abstract:BACKGROUND:Given that both insufficient and excess maternal iodine have adverse consequences such as poor cognitive performance, delayed physical development and increased fetal and infant mortality, the determination of maternal iodine status is very important. In this study, we established and verified a method invol...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2020.03.009
更新日期:2020-08-01 00:00:00
abstract:OBJECTIVE:Direct measurement of chemical constituents in complex biologic matrices without the use of analyte specific reagents could be a step forward toward the simplification of clinical biochemistry. Problems related to reagents such as production errors, improper handling, and lot-to-lot variations would be elimin...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2014.05.064
更新日期:2014-09-01 00:00:00
abstract::The structure of enzyme active sites and the nature of the catalytic process are reviewed. The impressive efficiency of these protein catalysts appears to stem from such factors as proximity and orientation of enzyme and substrate moieties, strain, and the occurrence of distinctive microenvironments within catalytic c...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/s0009-9120(76)80040-9
更新日期:1976-06-01 00:00:00
abstract:BACKGROUND:Reference intervals have traditionally been partitioned by age based on statistical significance and physiological relevance. However, analyte concentration does not change abruptly with age, but rather dynamically. In this study, we establish biochemical marker continuous reference intervals for a Canadian ...
journal_title:Clinical biochemistry
pub_type: 临床试验,杂志文章
doi:10.1016/j.clinbiochem.2019.08.010
更新日期:2019-11-01 00:00:00
abstract:OBJECTIVES:To evaluate platelet activating factor (PAF) levels, its metabolic enzymes activity and its associations with other inflammatory markers in heart failure (HF) patients. DESIGN AND METHODS:PAF, and two of its key biosynthetic enzymes [lyso-PAF acetyltransferase (lyso-PAF-AT) and DTT-insensitive CDP-choline:1...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.09.113
更新日期:2009-01-01 00:00:00
abstract:OBJECTIVE:The aim of this study was to find a pre-interventional marker with the capacity to predict in-stent restenosis (ISR). Considering the anti-atherosclerotic role of adiponectin (APO), an adipocytokine with anti-inflammatory, anti-proliferative, anti-oxidative and anti-thrombotic properties, low plasma levels of...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2008.09.109
更新日期:2008-12-01 00:00:00
abstract:OBJECTIVE:Develop a simple LC-MS-MS method for determination of 25-hydroxymetabolites of vitamin D2 and D3 in serum. DESIGN AND METHODS:0.1 mL serum was mixed with 0.3 mL deproteinizing mixture containing two deuterated internal standards. Two LC-MS-MS systems were used: Waters Premier with an ESI source and a Hypersi...
journal_title:Clinical biochemistry
pub_type: 杂志文章
doi:10.1016/j.clinbiochem.2011.08.1134
更新日期:2011-11-01 00:00:00