Abstract：
OBJECTIVE:Dyslipidemia is commonly seen in patients with end-stage renal disease (ESRD). This prospective study investigates whether small-sized high-density lipoprotein (HDL) particles alone or in combination with high sensitivity C-reactive protein (hsCRP) are independent determinants of ESRD mortality. DESIGN AND METHODS:We performed 36 months follow-up study in 122 haemodialysis (HD) patients. HDL size and subclass distribution were determined by gradient gel electrophoresis. Baseline characteristics of the patients were evaluated for the prediction of mortality. RESULTS:Cox regressions analysis showed that patients with small-sized HDL particles had 2.8-fold higher risk of lethal outcome (P<0.05). Concomitant presence of small-sized HDL particles and increased hsCRP concentration were significantly associated with reduced survival rate (HR=3.907; P<0.05). Observed relationships persisted after adjustment for serum lipid and lipoprotein concentrations. CONCLUSIONS:Our results indicate that small-sized HDL particles alone and combined with elevated hsCRP concentrations are independent predictors of reduced survival in HD patients.
journal_name
Clin Biochemjournal_title
Clinical biochemistryauthors
Vekic J,Zeljkovic A,Bogavac-Stanojevic N,Jelic-Ivanovic Z,Spasojevic-Kalimanovska V,Simic-Ogrizovic S,Dopsaj V,Spasic Sdoi
10.1016/j.clinbiochem.2011.02.002subject
Has Abstractpub_date
2011-06-01 00:00:00pages
635-41issue
8-9eissn
0009-9120issn
1873-2933pii
S0009-9120(11)00064-6journal_volume
44pub_type
杂志文章abstract：OBJECTIVE:Prior to initial distribution of Glucose-6-phosphate dehydrogenase (G6PD) proficiency testing (PT) materials, we evaluated G6PD enzyme stability in dried blood spots (DBS) under various temperature and humidity environments to develop storage and usage guidelines for our new materials. DESIGN & METHODS:We pr...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2017.05.003
更新日期：2017-10-01 00:00:00
abstract：:Proteomics, the analysis of expressed proteins, has been an important developing area of research for the past two decades [Anderson, NG, Anderson, NL. Twenty years of two-dimensional electrophoresis: past, present and future. Electrophoresis 1996;17:443-453]. Advances in technology have led to a rapid increase in app...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2004.09.002
更新日期：2004-11-01 00:00:00
abstract：OBJECTIVE:To develop a tool to assess the clinical accuracy of glucose meter performance using an insulin dosing protocol to assess the frequency and extent of error in insulin dose categories. METHODS:Retrospective comparison of 1815 glucose meter and central laboratory glucose results obtained from 1698 critically i...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2019.06.001
更新日期：2019-08-01 00:00:00
abstract：OBJECTIVE:Previous studies suggested that decreased serum vaspin levels were associated with coronary artery disease (CAD). The present study aimed to investigate the association between plasma vaspin levels and different states of CAD. DESIGN AND METHODS:A total of 162 patients with coronary angiography (CAG) proved ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2013.06.028
更新日期：2013-10-01 00:00:00
abstract：:Immunoglobulin levels are elevated in the acquired immune deficiency syndrome (AIDS) and one class, IgA, has been suggested as a marker of disease progression. IgA is composed of two subclasses, IgA1 and IgA2; circulating IgA1 is roughly 85% of the total in healthy individuals. We have attempted to determine if the su...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(91)80020-4
更新日期：1991-06-01 00:00:00
abstract：:Comparison of the turbidimetric immunoassay of haptoglobin with a reference method (the RID technique with appropriate correction for phenotype) clearly showed the turbidimetric assay to be phenotype-dependent. Correction factors for the three main phenotypes were calculated and reference values determined. The observ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(87)80006-1
更新日期：1987-08-01 00:00:00
abstract：OBJECTIVES:Microarray analysis of gene expression profiles of blood leukocytes has many potential clinical and research applications. DESIGN AND METHODS:We used the PAXgene Blood RNA System to prepare RNA from the whole blood of normal volunteers using two incubation times followed by gene expression profiling using t...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2004.03.013
更新日期：2004-09-01 00:00:00
abstract：OBJECTIVES:Analysis of serum/plasma methylmalonic acid (MMA) is important for the diagnosis and management of methylmalonic acidemia in pediatric populations. This work focuses on developing and validating a liquid chromatography tandem mass spectrometry (LC-MS/MS) method to monitor methylmalonic acidemia using a simpl...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2016.05.010
更新日期：2016-09-01 00:00:00
abstract：OBJECTIVES:Pseudoxanthoma elasticum (PXE) is a hereditary disorder predominantly affecting the skin, retina and vascular system. The aim of this study was to measure cell adhesion molecules in PXE patients. DESIGN AND METHODS:Soluble P-, E- and L-selectins were measured in 61 non-consanguineous PXE patients. The distr...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2007.12.009
更新日期：2008-04-01 00:00:00
abstract：OBJECTIVES:To evaluate impact of risk estimation parameters for screening for Down Syndrome during the first and second trimesters. METHODS:We prospectively examined for their performance in the prenatal prediction of trisomy 21, alphafetoprotein (AFP), unconjugated estriol (uE3), total human chorionic gonadotropin (h...
journal_title：Clinical biochemistry
pub_type： 临床试验,杂志文章
doi：10.1016/0009-9120(95)00021-z
更新日期：1995-08-01 00:00:00
abstract：OBJECTIVES:To examine the diagnostic validity of bone alkaline phosphatase (BAP) in serum (a marker of bone formation) and of urinary excretion of pyridinium cross-links (PYR) (a marker of bone resorption) for detection of bone metastases. DESIGN AND METHODS:Two heterogeneous immunoassays were employed for determinati...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(95)96621-a
更新日期：1995-10-01 00:00:00
abstract：OBJECTIVE:This study aimed to evaluate the effects of statin therapy on serum levels of antibodies to several specific heat shock proteins (HSPs) in dyslipidemic patients. DESIGN AND METHODS:Participants (n=102) were treated with simvastatin (40mg/day), or placebo in a randomized, double-blind, placebo-controlled, cro...
journal_title：Clinical biochemistry
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.clinbiochem.2010.09.016
更新日期：2011-02-01 00:00:00
abstract：OBJECTIVES:: To test if circulating forms of the soluble urokinase-type plasminogen activator receptor (suPAR) are potential biomarkers of plaque vulnerability. DESIGN AND METHODS:: Plasma concentrations of suPAR(I-III), suPAR(II-III) and uPAR(I) were measured by time-resolved fluorescence immunoassays in Caucasian pa...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.09.028
更新日期：2010-01-01 00:00:00
abstract：OBJECTIVE:The aim of this study was to find a pre-interventional marker with the capacity to predict in-stent restenosis (ISR). Considering the anti-atherosclerotic role of adiponectin (APO), an adipocytokine with anti-inflammatory, anti-proliferative, anti-oxidative and anti-thrombotic properties, low plasma levels of...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2008.09.109
更新日期：2008-12-01 00:00:00
abstract：OBJECTIVES:To assess the association of serum adiponectin and microvascular complications of diabetes in an urban south Indian type 2 diabetic population. DESIGN AND METHODS:Diabetic subjects [n=487] were included from Chennai Urban Rural Epidemiology Study (CURES). Four-field stereo retinal color photography was done...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2014.10.009
更新日期：2015-01-01 00:00:00
abstract：OBJECTIVES:To insure commutability (equivalence) between whole blood and plasma glucose values and to assess potential interferences in glucose oxidase methods. DESIGN AND METHODS:We compared plasma glucose results by the Ektachem 700 analyzer to glucose results on whole blood by the Nova Stat Profile 6, and by the YS...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(95)02010-1
更新日期：1996-02-01 00:00:00
abstract：:1. A procedure for the assay of triglycerides on the ABA-50 is described. 2. The Boehringer Mannheim Triglyceride (Fully Enzymatic method) reagent kit has been evaluated with this method and was found to be satisfactory. ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(77)93190-3
更新日期：1977-12-01 00:00:00
abstract：OBJECTIVES:Leptin is a multifunctional polypeptide produced primarily by adipocytes and associated with the occurrence of obesity and insulin resistance. Tumor necrosis factor-alpha (TNF-alpha), a polypeptide cytokine produced primarily by mononuclear phagocytes, plays a key role in the initiation of the inflammatory r...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(02)00314-4
更新日期：2002-06-01 00:00:00
abstract：OBJECTIVES:To investigate the predictive value of cystatin C among patients diagnosed with non-ST-elevation acute coronary syndrome (nSTE-ACS). DESIGN AND METHODS:Admission serum samples from 245 nSTE-ACS patients were measured with a novel cystatin C immunoassay based on a dry-reagent, double monoclonal design. Creat...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2012.02.012
更新日期：2012-05-01 00:00:00
abstract：OBJECTIVES:Despite the evidence that both homocysteine and cysteine are important risk factors for vascular disease and atherosclerosis no information are reported about their effective amount in plaque and on the relationship with the other low molecular weight thiols. DESIGN AND METHODS:We used capillary electrophor...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.02.007
更新日期：2009-06-01 00:00:00
abstract：:Core Alzheimer's Disease (AD) biomarkers are the cerebrospinal fluid (CSF) proteins amyloid β42 and β40, and the tau proteins, total and phosphorylated. Their use is recommended by research guidelines for diagnostic purpose and for stratification of patients for clinical trials. However, novel potential biomarkers are...
journal_title：Clinical biochemistry
pub_type： 杂志文章,评审
doi：10.1016/j.clinbiochem.2019.08.008
更新日期：2019-10-01 00:00:00
abstract：OBJECTIVE:Can biochip arrays identify which individuals with metastatic disease will respond to an anti-metastatic agent? DESIGN AND METHODS:Cytokine and cell adhesion arrays (Randox Ltd) were measured over 1 month in 9 research participants receiving CTCE-9908 in a Phase I/II study. RESULTS:Research participants wit...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2009.04.008
更新日期：2009-07-01 00:00:00
abstract：OBJECTIVE:To develop sensitive nonisotopic assays for protein-tyrosine phosphatase (PTP) activity. METHODS:The fluorometric assay is based on the fact that phosphotyrosine but not tyrosine forms highly fluorescent complexes with Tb3+. Thus, PTP activity can be followed by measuring the decrease of fluorescence due to ...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(95)02026-8
更新日期：1996-04-01 00:00:00
abstract：OBJECTIVE:Protein glutathionylation is considered an important post-translational modification in the pathogenesis of complex diseases. The aim of this study was to examine whether hemoglobin (Hb) is modified by reduced glutathione (GSH) via oxidation of the thiol groups present in diabetes and its associated microangi...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2005.06.009
更新日期：2005-10-01 00:00:00
abstract：OBJECTIVES:We investigated whether plasma visfatin and binding protein-4 (RBP-4) levels correlate with obesity and type 2 diabetes mellitus (T2DM). DESIGN AND METHODS:Two groups were enrolled: Group 1: 40 patients with T2DM and Group 2: 40 age- and gender-matched healthy controls. Both groups were subdivided according...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2011.08.1148
更新日期：2011-12-01 00:00:00
abstract：OBJECTIVES:Characterization of beta-galactosidase in leukocytes and fibroblasts of heterozygotes for GM1 type I. DESIGN AND METHODS:Leukocyte and fibroblast beta-galactosidase activity was determined fluorimetrically using 4-methylumbelliferyl-beta-D-galactoside as an artificial substrate. Optimum pH, Km, Vmax and the...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(00)00049-7
更新日期：2000-03-01 00:00:00
abstract：OBJECTIVES:During the development of a testosterone assay by LC-MS/MS, we encountered significant assay interference introduced by blood collection tubes. We examined a number of commonly used blood collection tubes for the presence of interference and its impact on testosterone quantitation. DESIGN AND METHODS:A numb...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2012.08.008
更新日期：2012-12-01 00:00:00
abstract：OBJECTIVES:To establish whether normal human subjects excrete glycine betaine at a constant rate. DESIGN AND METHODS:Urine was collected from ten normal healthy male subjects for 14 days, during which fluid intake was systematically varied from <800 mL to >3 L per day. Glycine betaine, sorbitol and creatinine excretio...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/j.clinbiochem.2006.10.029
更新日期：2007-04-01 00:00:00
abstract：:1. A relatively inexpensive semi-automated enzymatic procedure is described for the determination of total glycerol with an Auto Analyzer. Free glycerol is determined without saponification in a deproteinized supernatant. 2. The dilution of triglyceride samples during preparation is such that the samples during prepar...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/s0009-9120(77)90232-6
更新日期：1977-02-01 00:00:00
abstract：:Epidemiological evidence suggests that the ratio HDL-cholesterol (HDL-C)/total cholesterol (TC) or apolipoprotein A1 (apo A1)/apolipoprotein B (apo B) are good indicators of coronary heart disease risk. In investigating the distribution of these ratios in the typical population served by our routine laboratory, we ana...
journal_title：Clinical biochemistry
pub_type： 杂志文章
doi：10.1016/0009-9120(92)80004-z
更新日期：1992-10-01 00:00:00
