Effects of divalent cations on bovine testicular hyaluronidase catalyzed transglycosylation of chondroitin sulfates.

abstract：:We generated two transgenic mice carrying the adenovirus type 12 E1 region genes under control of the human renin promoter, in which the E1A and E1B genes were expressed predominantly in the kidney. Interestingly, renal transcription of the gene for mouse renin was shown to be elevated at 28 and 40 folds as compared i...

journal_title：Biochemical and biophysical research communications

authors： Fukamizu A,Sagara M,Sugiyama F,Yagami K,Murakami K

更新日期：1994-02-28 00:00:00

abstract：:Dual-excitation ratiometric dyes permit quantitative Ca2+ measurements by minimizing the effects of several artifacts that are unrelated to changes in the concentration of free Ca2+ ([Ca2+]). These dyes are excited alternately at two different wavelengths, and the pair of intensity measurements must be collected seque...

journal_title：Biochemical and biophysical research communications

authors： Fukano T,Shimozono S,Miyawaki A

更新日期：2004-04-23 00:00:00

abstract：:In Chinese hamster ovary cells, [3H]formycin B is metabolized into formycin B-5'-monophosphate, formycin A-5'-monophosphate and higher phosphorylated derivatives of formycin A which are incorporated into RNA. Mutants of CHO cells independently selected for resistance to various adenosine analogs viz. toyocamycin, tube...

journal_title：Biochemical and biophysical research communications

authors： Mehta KD,Gupta RS

更新日期：1985-07-31 00:00:00

abstract：:The coding sequence of type II phospholipase A2 from human placenta was cloned in a bovine papilloma virus-derived eukaryotic expression vector under the control of the metallothionein promoter. Stably transfected C127 mouse fibroblast lines were obtained with this vector. These transfected cells overexpressed a funct...

journal_title：Biochemical and biophysical research communications

authors： Pernas P,Masliah J,Olivier JL,Salvat C,Rybkine T,Bereziat G

更新日期：1991-08-15 00:00:00

abstract：:The aim of this study was to investigate the possible pathological relation between mechanical stress and hyperpigmentation. We did this by investigating the influence of cyclic stretch on the expression of keratinocyte- and fibroblast-derived melanogenetic paracrine cytokines in vitro. Using primary human keratinocyt...

journal_title：Biochemical and biophysical research communications

authors： Kurita M,Okazaki M,Fujino T,Takushima A,Harii K

更新日期：2011-05-27 00:00:00

abstract：BACKGROUND:Resistin is associated with insulin resistance, and determining its developmental and molecular mechanisms may help the development of novel treatments. MicroRNAs (miRNAs) are involved in many physiological and pathological processes as negative regulators. However, it remains unclear whether miRNAs play a r...

journal_title：Biochemical and biophysical research communications

authors： Wen F,Yang Y,Jin D,Sun J,Yu X,Yang Z

更新日期：2014-03-07 00:00:00

abstract：:Extracellular acidic pH-activated chloride channel I(Cl, acid), has been characterized in HEK 293 cells and mammalian cardiac myocytes. This study was designed to characterize I(Cl,acid) in human umbilical vein endothelial cells(HUVECs). The activation and deactivation of the current rapidly and repeatedly follows the...

journal_title：Biochemical and biophysical research communications

authors： Ma ZY,Zhang W,Chen L,Wang R,Kan XH,Sun GZ,Liu CX,Li L,Zhang Y

更新日期：2008-07-04 00:00:00

abstract：:Nucleophosmin (NPM) is a multifunctional nucleolar protein that has been linked with nucleolar stress. In non-neuronal cell lines, NPM may enhance or inhibit the activity of tumor suppressor p53, a major apoptotic protein. The relationship between NPM and p53 in the central nervous system (CNS) remains unknown. Here, ...

journal_title：Biochemical and biophysical research communications

authors： Marquez-Lona EM,Tan Z,Schreiber SS

更新日期：2012-01-06 00:00:00

abstract：:The disruption of the intracellular Ca(2+) homeostasis of Leishmania mexicana represents a major target for the action of drugs, such as amiodarone and miltefosine. However, little is known about the mechanism of Ca(2+) entry to these cells. Here we show the presence of a Ca(2+) channel in the plasma membrane of these...

journal_title：Biochemical and biophysical research communications

authors： Benaim G,García-Marchán Y,Reyes C,Uzcanga G,Figarella K

更新日期：2013-01-18 00:00:00

abstract：:Neuroblastoma LAN-5 cells exposed to retinoic acid cease to multiply and extend neurite outgrowths acquiring a neuronal phenotype. We now report that protein kinase C-theta; (PKC-theta;) isozyme is involved in this differentiation process due to the following findings: (i) PKC-theta; is expressed by LAN-5 cells as a n...

journal_title：Biochemical and biophysical research communications

authors： Sparatore B,Patrone M,Passalacqua M,Pedrazzi M,Pontremoli S,Melloni E

更新日期：2000-12-20 00:00:00

abstract：:Recent years have seen considerable evolution in our understanding of the mechanisms of oxygen activation by non-heme iron enzymes, with high-valent iron-oxo intermediates coming to the forefront as formidably potent oxidants. In the absence of substrate, the generation of vividly colored chromophores deriving from th...

journal_title：Biochemical and biophysical research communications

authors： Farquhar ER,Koehntop KD,Emerson JP,Que L Jr

更新日期：2005-12-09 00:00:00

abstract：:Previous studies have shown that DNA topoisomerase Iα (AtTOP1α) has specific developmental functions during growth and development in Arabidopsis thaliana. However, little is known about the roles of DNA topoisomerases in the closely related and commercially important plant, rapeseed (Brassica napus). Here, the full-l...

journal_title：Biochemical and biophysical research communications

authors： Gao C,Qi S,Liu K,Li D,Jin C,Duan S,Zhang M,Chen M

更新日期：2017-04-22 00:00:00

abstract：:Directed cell migration in tissues mediates various physiological processes and is guided by complex cellular factors such as chemoattractant gradients and electric fields. Direct current (DC) electric fields can be generated in physiological settings and the electric field guided migration of various cell types (i.e....

journal_title：Biochemical and biophysical research communications

authors： Wu D,Lin F

更新日期：2011-08-12 00:00:00

abstract：:Arctigenin is a bioactive constituent from dried seeds of Arctium lappa L., which was traditionally used as medicine. Arctigenin exhibits various bioactivities, but its effects on blood pressure regulation are still not widely studied. In this study, we investigated antihypertensive effects of arctigenin by long-term ...

journal_title：Biochemical and biophysical research communications

authors： Liu Y,Wang G,Yang M,Chen H,zhao Y,Yang S,Sun C

更新日期：2015-12-25 00:00:00

abstract：:Wnt-1, the vertebrate counterpart of the Drosophila wingless gene, plays an important role in the early morphogenesis of neural tissues. In this report, we have shown that overexpression of Wnt-1 can direct embryonic carcinoma P19 cells to differentiate into neuron-like cells in the absence of retinoic acid. Immunocyt...

journal_title：Biochemical and biophysical research communications

authors： Tang K,Yang J,Gao X,Wang C,Liu L,Kitani H,Atsumi T,Jing N

更新日期：2002-04-26 00:00:00

abstract：:The complete covalent structure of the gamma 2 subunit of bovine brain G proteins was determined by peptide mapping using matrix-assisted laser desorption ionization and electrospray ionization mass spectrometry and by partial sequencing using tandem mass spectrometry. Fragments were identified in proteolytic digests ...

journal_title：Biochemical and biophysical research communications

authors： Wilcox MD,Schey KL,Busman M,Hildebrandt JD

更新日期：1995-07-17 00:00:00

abstract：:N-(p-amylcinnamoyl)anthranilic acid (ACA), a phospholipase A(2) (PLA(2)) inhibitor, is structurally-related to non-steroidal anti-inflammatory drugs (NSAIDs) of the fenamate group and may also modulate various ion channels. We used the whole-cell, patch-clamp technique at room temperature to investigate the effects of...

journal_title：Biochemical and biophysical research communications

authors： Gwanyanya A,Macianskiene R,Bito V,Sipido KR,Vereecke J,Mubagwa K

更新日期：2010-11-19 00:00:00

abstract：:Arctigenin (ARC), a phenylpropanoid dibenzylbutyrolactone lignan derived from Arctium lappa L, has been reported to protect against cerebral ischemia injury in rats, but the underlying mechanism is unclear. In this study, we investigated whether ARC ameliorated ischemic stroke by inhibiting NLRP3 inflammasome-derived ...

journal_title：Biochemical and biophysical research communications

authors： Zhang S,Jiang L,Che F,Lu Y,Xie Z,Wang H

更新日期：2017-11-04 00:00:00

abstract：:Consumption of a single serving of tomatoes by healthy human volunteers was sufficient to alter levels of oxidative DNA base damage in white cell DNA within 24 h. Levels of the mutagenic oxidized purine base 8-hydroxyguanine decreased, especially in those subjects whose initial levels of this base were higher than the...

journal_title：Biochemical and biophysical research communications

authors： Rehman A,Bourne LC,Halliwell B,Rice-Evans CA

更新日期：1999-09-07 00:00:00

abstract：:Ras can become activated via multiple distinct receptors in T lymphocytes. However, mechanistic studies of Ras signaling in normal T cells have been hampered by the lack of an efficient technology for gene transfer into resting post-thymic cells. We have overcome this limitation by utilizing adenoviral transduction of...

journal_title：Biochemical and biophysical research communications

authors： Marks RE,Ho AW,Rivas F,Marshall E,Janardhan S,Gajewski TF

更新日期：2003-12-19 00:00:00

abstract：:It is widely accepted that applying exogenous photosensitizers in living organism the photodynamic effect is mediated by singlet oxygen formed by energy transfer from excited sensitizer to oxygen present in the cells (Type II mechanism). Though in homogeneous solutions singlet oxygen was detected during sensitization,...

journal_title：Biochemical and biophysical research communications

authors： Gál D

更新日期：1994-07-15 00:00:00

abstract：:Lanosterol synthase [(S)-2,3-epoxysqualene mutase (cyclizing, lanosterol forming), EC 5.4.99.7] catalyzes the cyclization of (S)-2,3-oxidosqualene to lanosterol in the reaction that forms the sterol nucleus. We report herein the cloning and characterization of the human gene (OSC) encoding lanosterol synthase, a predi...

journal_title：Biochemical and biophysical research communications

authors： Baker CH,Matsuda SP,Liu DR,Corey EJ

更新日期：1995-08-04 00:00:00

abstract：:The cDNA coding for the signal peptide of rat liver mitochondrial aldehyde dehydrogenase was sequenced. The deduced amino acid sequence of the signal peptide was MLRAALSTARRGPRLSRLL. From this sequence an amphiphilic helix which had a high hydrophobic moment could be constructed. A comparison to the published cDNA seq...

journal_title：Biochemical and biophysical research communications

authors： Farrés J,Guan KL,Weiner H

更新日期：1988-02-15 00:00:00

abstract：:An in organello footprinting approach has been used to probe a protein-DNA interaction of a nuclear coded 25 kDa protein, previously isolated in our laboratory, that binds "in vitro" a region within the ND2 gene, located upstream of the Ori-L. Footprinting studies with the purine-modifying reagent dimethyl sulfate and...

journal_title：Biochemical and biophysical research communications

authors： Cingolani G,Capaccio L,D'Elia D,Gadaleta G

更新日期：1997-02-24 00:00:00

abstract：:We investigated by RNase protection the differential expression of CYP3A4 and CYP3A7 mRNAs in fetal and adult human livers and in adult hepatocytes in primary culture. Our results show that CYP3A7 is expressed in the liver of 8 of 9 adult Caucasians examined, at an average level of 1.7% of the level found in a fetal l...

journal_title：Biochemical and biophysical research communications

authors： Greuet J,Pichard L,Bonfils C,Domergue J,Maurel P

更新日期：1996-08-14 00:00:00

abstract：:First messengers and viral transfection are the two most common ways to stimulate cells for signal output, although their applications are limited. We investigated mechanisms of inducing neural stem cell differentiation using recombinant ephrin-B2/Fc and found that it acted as a ligand and inhibited endogenous ephrin-...

journal_title：Biochemical and biophysical research communications

authors： Xiaodong H,Zhen H,Min S,Zhiming C,Hongyan J,Chong Z,Xuefeng T,Guohua J

更新日期：2013-10-18 00:00:00

abstract：:Rhodamine 123, a laser dye, has been demonstrated to inhibit import of the precursor to pyridine dinucleotide transhydrogenase into mitochondria in rat liver cells. When rat hepatocytes were labeled with 35[S] methionine in the presence of 0.4 mM rhodamine 123, the precursor to transhydrogenase was found to have a hal...

journal_title：Biochemical and biophysical research communications

authors： Lubin IM,Wu LN,Wuthier RE,Fisher RR

更新日期：1987-04-14 00:00:00

abstract：:We screened various isoprenoids to search for inducers of apoptosis-like cell death ("apoptosis") in the shoot primordia of Matricaria chamomilla and found that geraniol has the most potent apoptosis-inducing activity among terpenoids, as judged from DNA fragmentation in the cells. The apoptosis-inducing activity of g...

journal_title：Biochemical and biophysical research communications

authors： Izumi S,Takashima O,Hirata T

更新日期：1999-06-16 00:00:00

abstract：:The solubilized [3H]ryanodine receptor from cardiac sarcoplasmic reticulum was centrifuged through linear sucrose gradients. A single peak of radioactivity with apparent sedimentation coefficient of approximately 30S specifically comigrated with a high molecular weight protein of apparent relative molecular mass appro...

journal_title：Biochemical and biophysical research communications

authors： Lai FA,Anderson K,Rousseau E,Liu QY,Meissner G

更新日期：1988-02-29 00:00:00

abstract：:The present study demonstrates that human platelets possess a specific L-arginine transport system able to provide adequate amounts of L-arginine for endogenous nitric oxide production. L-arginine uptake takes place through a saturable high affinity carrier-mediated Na(+)-independent process which is significantly inh...

journal_title：Biochemical and biophysical research communications

authors： Vasta V,Meacci E,Farnararo M,Bruni P

更新日期：1995-01-26 00:00:00