Gestational changes in pulmonary converting enzyme activity in the fetal rabbit.


:Changes in angiotensin-converting enzyme were measured in the lungs of fetal rabbits isolated and perfused in situ at varying ages from 22 days gestation to 7 days of age under controlled conditions of flow, pH, and temperature. Enzyme activity was assessed by infusing bradykinin or angiotensin I in Krebs-Henseleit solution and measuring residual peptide in the effluent by radioimmunoassay. The levels of substrate studied were below those required for enzyme saturation. Lungs of 22 day gestation fetuses removed only one-third of either peptide. The activity at term and in neonatal life resulted in more than 80% peptide removal. The time of the greatest rise in the percent substrate cleared occurs earlier than the time of the greatest increase in lung and body weight. The lower percentage of substrate cleared in early gestation appears to result in part from a limited surface area for enzyme activity in the primitive fetal pulmonary microvascular bed, since morphological studies with fluorescein-tagged anticonverting enzyme antibody demonstrated the presence of enzyme in the lung as early as 17 days of gestation. Electron micrographs of the pulmonary endothelial cell surface reveal that the degree of surface infolding and hence surface area increases with gestation. The higher percentage of substrate cleared in later gestation closely parallels the structural and ultrastructural development of the vascular bed. The presence of converting enzyme in the placenta by the second third of gestation and the large size of the placenta suggest that this organ may be a major locus of converting enzyme activity in the fetus.


Circ Res


Circulation research


Stalcup SA,Pang LM,Lipset JS,Odya CE,Goodfriend TL,Mellins RB




Has Abstract


1978-11-01 00:00:00












  • Expression of mutant p193 and p53 permits cardiomyocyte cell cycle reentry after myocardial infarction in transgenic mice.

    abstract::Previous studies have demonstrated that expression of p193 and p53 mutants with dominant-interfering activities renders embryonic stem cell-derived cardiomyocytes responsive to the growth promoting activities of the E1A viral oncoproteins. In this study, the effects of p53 and p193 antagonization on cardiomyocyte cell...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Nakajima H,Nakajima HO,Tsai SC,Field LJ

    更新日期:2004-06-25 00:00:00

  • An osteopontin-NADPH oxidase signaling cascade promotes pro-matrix metalloproteinase 9 activation in aortic mesenchymal cells.

    abstract::Osteopontin (OPN) is a cytokine upregulated in diabetic vascular disease. To better understand its role in vascular remodeling, we assessed how OPN controls metalloproteinase (MMP) activation in aortic adventitial myofibroblasts (AMFs) and A7r5 vascular smooth muscle cells (VSMCs). By zymography, OPN and tumor necrosi...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Lai CF,Seshadri V,Huang K,Shao JS,Cai J,Vattikuti R,Schumacher A,Loewy AP,Denhardt DT,Rittling SR,Towler DA

    更新日期:2006-06-23 00:00:00

  • Nitroxyl-mediated disulfide bond formation between cardiac myofilament cysteines enhances contractile function.

    abstract:RATIONALE:In the myocardium, redox/cysteine modification of proteins regulating Ca(2+) cycling can affect contraction and may have therapeutic value. Nitroxyl (HNO), the one-electron-reduced form of nitric oxide, enhances cardiac function in a manner that suggests reversible cysteine modifications of the contractile ma...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Gao WD,Murray CI,Tian Y,Zhong X,DuMond JF,Shen X,Stanley BA,Foster DB,Wink DA,King SB,Van Eyk JE,Paolocci N

    更新日期:2012-09-28 00:00:00

  • CD40 is constitutively expressed on platelets and provides a novel mechanism for platelet activation.

    abstract::CD40 is a 48-kDa phosphorylated transmembrane glycoprotein belonging to the TNF receptor superfamily. CD40 has been demonstrated on a range of cell types, and it has an important role in adaptive immunity and inflammation. CD40 has recently been described on platelets but platelet activation by CD40 has not been descr...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Inwald DP,McDowall A,Peters MJ,Callard RE,Klein NJ

    更新日期:2003-05-16 00:00:00

  • Duration and Life-Stage of Antibiotic Use and Risks of All-Cause and Cause-Specific Mortality: Prospective Cohort Study.

    abstract::Rationale: The overuse of antibiotics has been an important clinical issue, and antibiotic exposure is linked to alterations in gut microbiota, which has been related to risks of various chronic diseases such as cardiovascular disease and cancer. Also, duration of antibiotic exposure may be a risk factor of premature ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Heianza Y,Ma W,Li X,Cao Y,Chan AT,Rimm EB,Hu FB,Rexrode KM,Manson JE,Qi L

    更新日期:2020-01-31 00:00:00

  • Increased thromboxane biosynthesis during coronary thrombolysis. Evidence that platelet activation and thromboxane A2 modulate the response to tissue-type plasminogen activator in vivo.

    abstract::Platelet activation is markedly increased during coronary thrombolysis and limits the response to thrombolytic therapy. A possible mediator of platelet activation in this setting is thromboxane (TX) A2, a potent platelet agonist formed in greatly increased amounts during coronary thrombolysis in man. To address this h...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Fitzgerald DJ,Wright F,FitzGerald GA

    更新日期:1989-07-01 00:00:00

  • Dicer cleavage by calpain determines platelet microRNA levels and function in diabetes.

    abstract:RATIONALE:MicroRNAs (miRNAs) are short noncoding RNA species generated by the processing of longer precursors by the ribonucleases Drosha and Dicer. Platelets contain large amounts of miRNA that are altered by disease, in particular diabetes mellitus. OBJECTIVE:This study determined why platelet miRNA levels are atten...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Elgheznawy A,Shi L,Hu J,Wittig I,Laban H,Pircher J,Mann A,Provost P,Randriamboavonjy V,Fleming I

    更新日期:2015-07-03 00:00:00

  • Fcγ receptors and ligands and cardiovascular disease.

    abstract::Fcγ receptors (FcγRs) classically modulate intracellular signaling on binding of the Fc region of IgG in immune response cells. How FcγR and their ligands affect cardiovascular health and disease has been interrogated recently in both preclinical and clinical studies. The stimulation of activating FcγR in endothelial ...

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: Tanigaki K,Sundgren N,Khera A,Vongpatanasin W,Mineo C,Shaul PW

    更新日期:2015-01-16 00:00:00

  • Efficient gene disruption in cultured primary human endothelial cells by CRISPR/Cas9.

    abstract:RATIONALE:The participation of endothelial cells (EC) in many physiological and pathological processes is widely modeled using human EC cultures, but genetic manipulation of these untransformed cells has been technically challenging. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated p...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Abrahimi P,Chang WG,Kluger MS,Qyang Y,Tellides G,Saltzman WM,Pober JS

    更新日期:2015-07-03 00:00:00

  • Myocardial function of the interventricular septum. Effects of right and left ventricular pressure loading before and after pericardiotomy in dogs.

    abstract::We examined the function of the interventricular septum in six open-chest dogs by inserting piezoelectric crystals into the interventricular septum. Continuous ultrasonic recordings showed that the changes in myocardial chord length (MCL) in the septum and free walls of the right and left ventricle were similar during...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Molaug M,Stokland O,Ilebekk A,Lekven J,Kiil F

    更新日期:1981-07-01 00:00:00

  • Direct neurohumoral evidence for isolated sympathetic nervous system activation to skeletal muscle in response to cardiopulmonary baroreceptor unloading.

    abstract::It has been postulated that cardiopulmonary baroreceptor unloading in humans results in nonuniform activation of the sympathetic nervous system. We reasoned that simultaneous measurements of arterial and venous norepinephrine (NE) spillover and clearance (using NE kinetics), muscle sympathetic neural activity (using m...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Baily RG,Prophet SA,Shenberger JS,Zelis R,Sinoway LI

    更新日期:1990-06-01 00:00:00

  • Receptor-Mediated Mechanism Controlling Tissue Levels of Bioactive Lipid Oxidation Products.

    abstract:RATIONALE:Oxidative stress is an important contributing factor in several human pathologies ranging from atherosclerosis to cancer progression; however, the mechanisms underlying tissue protection from oxidation products are poorly understood. Oxidation of membrane phospholipids, containing the polyunsaturated fatty ac...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Kim YW,Yakubenko VP,West XZ,Gugiu GB,Renganathan K,Biswas S,Gao D,Crabb JW,Salomon RG,Podrez EA,Byzova TV

    更新日期:2015-07-31 00:00:00

  • Interaction of acidosis and increased extracellular potassium on action potential characteristics and conduction in guinea pig ventricular muscle.

    abstract::We studied the individual and combined effects of extracellular acidosis and increases in extracellular potassium on action potential characteristics and conduction in order to gain a better understanding of the effects of acute ischemia. At each level of potassium between 2.7 and 17 mm, acidosis induced by increasing...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Kagiyama Y,Hill JL,Gettes LS

    更新日期:1982-11-01 00:00:00

  • Rho kinase-mediated vasoconstriction is important in severe occlusive pulmonary arterial hypertension in rats.

    abstract::Vascular remodeling, rather than vasoconstriction, is believed to account for high vascular resistance in severe pulmonary arterial hypertension (PAH). We have found previously that acute Rho kinase inhibition nearly normalizes PAH in chronically hypoxic rats that have no occlusive neointimal lesions. Here we examined...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Oka M,Homma N,Taraseviciene-Stewart L,Morris KG,Kraskauskas D,Burns N,Voelkel NF,McMurtry IF

    更新日期:2007-03-30 00:00:00

  • Limitations of the double sucrose gap voltage clamp technique in tension-voltage determinations on frog atrial muscle.

    abstract::The purpose of this study was to evaluate the limitations of the double sucrose gap voltage clamp technique in the determination of tension-voltage relationships for frog atrial muscle. Tension-voltage relationships were determined under two conditions. In one case we determined both the tension response and slow inwa...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Tarr M,Trank JW

    更新日期:1976-07-01 00:00:00

  • Genetics of congenital heart disease: the glass half empty.

    abstract::Congenital heart disease (CHD) is the most common congenital anomaly in newborn babies. Cardiac malformations have been produced in multiple experimental animal models, by perturbing selected molecules that function in the developmental pathways involved in myocyte specification, differentiation, or cardiac morphogene...

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: Fahed AC,Gelb BD,Seidman JG,Seidman CE

    更新日期:2013-02-15 00:00:00

  • Molecular cloning and characterization of the intermediate-conductance Ca(2+)-activated K(+) channel in vascular smooth muscle: relationship between K(Ca) channel diversity and smooth muscle cell function.

    abstract::Recent evidence suggests that functional diversity of vascular smooth muscle is produced in part by a differential expression of ion channels. The aim of the present study was to examine the role of Ca(2+)-activated K(+) channels (K(Ca) channels) in the expression of smooth muscle cell functional phenotype. We found t...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Neylon CB,Lang RJ,Fu Y,Bobik A,Reinhart PH

    更新日期:1999-10-29 00:00:00

  • Human activin-A is expressed in the atherosclerotic lesion and promotes the contractile phenotype of smooth muscle cells.

    abstract::Activin is a member of the transforming growth factor-beta superfamily, and it modulates the proliferation and differentiation of various target cells. In this study, we investigated the role of activin in the initiation and progression of human atherosclerosis. The expression of activin, its physiological inhibitor f...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Engelse MA,Neele JM,van Achterberg TA,van Aken BE,van Schaik RH,Pannekoek H,de Vries CJ

    更新日期:1999-11-12 00:00:00

  • Erythrocyte Metabolic Reprogramming by Sphingosine 1-Phosphate in Chronic Kidney Disease and Therapies.

    abstract:RATIONALE:Hypoxia promotes renal damage and progression of chronic kidney disease (CKD). The erythrocyte is the only cell type for oxygen (O2) delivery. Sphingosine 1-phosphate (S1P)-a highly enriched biolipid in erythrocytes-is recently reported to be induced under high altitude in normal humans to enhance O2 delivery...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Xie T,Chen C,Peng Z,Brown BC,Reisz JA,Xu P,Zhou Z,Song A,Zhang Y,Bogdanov MV,Kellems RE,D'Alessandro A,Zhang W,Xia Y

    更新日期:2020-07-17 00:00:00

  • Antithrombotic Agents.

    abstract::Recent advances in our understanding of the contribution of thrombin generation to arterial thrombosis and the role of platelets in venous thrombosis have prompted new treatment paradigms. Nonetheless, bleeding remains the major side effect of such treatments spurring the quest for new antithrombotic regimens with bet...

    journal_title:Circulation research

    pub_type: 杂志文章,评审


    authors: Chan NC,Weitz JI

    更新日期:2019-02-01 00:00:00

  • Angiogenic function of prostacyclin biosynthesis in human endothelial progenitor cells.

    abstract::The role of prostaglandin production in the control of regenerative function of endothelial progenitor cells (EPCs) has not been studied. We hypothesized that activation of cyclooxygenase (COX) enzymatic activity and the subsequent production of prostacyclin (PGI(2)) is an important mechanism responsible for the regen...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: He T,Lu T,d'Uscio LV,Lam CF,Lee HC,Katusic ZS

    更新日期:2008-07-03 00:00:00

  • Ultrastructural morphometric analysis of myocardium from dogs, rats, hamsters, mice, and from human hearts.

    abstract::Volume densities of mitochondria, myofibrils, and unspecified cytoplasm were measured by ultrastructural morphometry in myocardium from dogs, rats, hamsters, mice, and in biopsied tissue from human hearts. Human myocardium was composed of 23% mitochondria, 59% myofibrils, and 18% cytoplasm. Volume densities for mitoch...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Schaper J,Meiser E,Stämmler G

    更新日期:1985-03-01 00:00:00

  • Myocyte growth without physiological impairment in gradually induced rat cardiac hypertrophy.

    abstract::A surgical technique was developed to place sutures around the pulmonary arteries of young rats (about 1 month old and 100 g body weight). As the operated rats grew, the pulmonary arteries were gradually constricted, leading after 4-6 weeks to the development of severe right ventricular hypertrophy with free wall weig...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Julian FJ,Morgan DL,Moss RL,Gonzalez M,Dwivedi P

    更新日期:1981-12-01 00:00:00

  • TRPC1 channels are critical for hypertrophic signaling in the heart.

    abstract:RATIONALE:Cardiac muscle adapts to increase workload by altering cardiomyocyte size and function resulting in cardiac hypertrophy. G protein-coupled receptor signaling is known to govern the hypertrophic response through the regulation of ion channel activity and downstream signaling in failing cardiomyocytes. OBJECTI...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Seth M,Zhang ZS,Mao L,Graham V,Burch J,Stiber J,Tsiokas L,Winn M,Abramowitz J,Rockman HA,Birnbaumer L,Rosenberg P

    更新日期:2009-11-06 00:00:00

  • Limited maximal vasodilator capacity of forearm resistance vessels in normotensive young men with a familial predisposition to hypertension.

    abstract::The study was performed to determine whether there is a structural vascular abnormality in normotensive subjects with hypertensive relatives. We examined maximal vasodilator capacity of forearm resistance vessels in 23 normotensive young men (mean blood pressure 94 +/- 0.4 mm Hg, mean +/- SE) with hypertensive relativ...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Takeshita A,Imaizumi T,Ashihara T,Yamamoto K,Hoka S,Nakamura M

    更新日期:1982-05-01 00:00:00

  • Effects of inotropic and arrhythmogenic digoxin doses and of digoxin-specific antibody on myocardial monovalent cation transport in the dog.

    abstract::The effects of digoxin on monovalent cation active transport were determined in cardiac tissue obtained from dogs given inotropic, toxic, or lethal doses of digoxin. In hemodynamically monitored dogs, active uptake of the K+ analogue Rb+ was determined in vitro in a control myocardial biopsy, and then in serial biopsi...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Hougen TJ,Lloyd BL,Smith TW

    更新日期:1979-01-01 00:00:00

  • Tissue- and species-specific expression of inhibitory guanine nucleotide-binding proteins. Cloning of a full-length complementary DNA from canine heart.

    abstract::Three different isoforms of the inhibitory guanine nucleotide-binding protein alpha-subunit (Gi alpha) have been cloned. However, little information on the cardiac-specific expression of these isoforms is available. The deduced amino acid sequence of a full-length Gi alpha 2 complementary DNA from a canine ventricular...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Holmer SR,Stevens S,Homcy CJ

    更新日期:1989-10-01 00:00:00

  • The effect of hypoxia on the regional distribution of cardiac output in the dog.

    abstract::Twenty-one dogs were studied under conditions of normal oxygenation and hypoxia with the microsphere distribution method to determine the effect of arterial oxygen saturation on the regional distribution of cardiac output. The dogs were anesthetized and artifically ventilated. Cannulas were placed in the left ventricl...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Adachi H,Strauss W,Ochi H,Wagner HN Jr

    更新日期:1976-09-01 00:00:00

  • Sphingosylphosphorylcholine induces a hypertrophic growth response through the mitogen-activated protein kinase signaling cascade in rat neonatal cardiac myocytes.

    abstract::The sphingolipid metabolites, sphingosine (SPH), SPH 1-phosphate (S1P), and sphingosylphosphorylcholine (SPC), can act as intracellular as well as extracellular signaling molecules. These compounds have been implicated in the regulation of cell growth, differentiation, and programmed cell death in nonmyocytes, but the...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Sekiguchi K,Yokoyama T,Kurabayashi M,Okajima F,Nagai R

    更新日期:1999-11-26 00:00:00

  • Reversibly injured, postischemic canine myocardium retains normal contractile reserve.

    abstract::Transient coronary occlusion (15 minutes) does not result in irreversible myocardial injury but is associated with a depression of contractile function sustained for several hours to days ("stunned myocardium"). The defect in the contractile process responsible for this phenomenon has been suggested to be causally rel...

    journal_title:Circulation research

    pub_type: 杂志文章


    authors: Ito BR,Tate H,Kobayashi M,Schaper W

    更新日期:1987-12-01 00:00:00