Expression of N-methyl D-aspartate receptor subunits in amoeboid microglia mediates production of nitric oxide via NF-κB signaling pathway and oligodendrocyte cell death in hypoxic postnatal rats.


:The present study was focused on identifying the expression of N-methyl D-aspartate receptor (NMDAR) subunits on activated microglia and to determine their role in the pathogenesis of periventricular white matter damage (PWMD) in neonatal rats following hypoxia. One day old wistar rats were subjected to hypoxia (5% O(2) ; 95% N(2) ) and the mRNA and protein expression of NMDAR subunits (NR1, NR2A-D, and NR3A) in the periventricular white matter (PWM) was determined at different time points (3,24 h, 3, 7, and 14 days) following hypoxic exposure. Immunoexpression of NR1 and NR2A-D was localized in amoeboid microglial cells (AMC) suggesting the presence of functional NMDARs in them. The expression of NMDAR in primary microglial cultures was ascertained by RT-PCR analysis and double immunofluorescence studies. The functionality of the microglial NMDAR in cultured microglial cells was examined by monitoring calcium movements in cells with fura-2. In primary microglial cultures, hypoxia induced the nuclear translocation of NF-κB which was suppressed by administration of MK801, an NMDAR antagonist. MK801 also down regulated the hypoxia-induced expression of tumor necrosis factor-α, interleukin-1β, inducible nitric oxide synthase (iNOS), and nitric oxide (NO) production by microglia which may be mediated by the NF-κB signaling pathway. NO produced by microglia is known to cause death of oligodendrocytes in the developing PWM. In this connection, pharmacological agents such as MK801, BAY (NF-κB inhibitor), and 1400w (iNOS inhibitor) proved to be beneficial since they reduced the hypoxia-induced iNOS expression, NO production, and a corresponding reduction in the death of oligodendrocytes following hypoxia.






Murugan M,Sivakumar V,Lu J,Ling EA,Kaur C




Has Abstract


2011-04-01 00:00:00














  • Expression of the complement C3a and C5a receptors after permanent focal ischemia: An alternative interpretation.

    abstract::The receptors for the complement anaphylatoxins C3a and C5a are expressed by glial cells and neurons in normal and inflamed brain. Previous studies demonstrated modest elevations in mRNA expression of these receptors in a model of focal cerebral ischemia. Using a similar model system for both mice and rats, we report ...


    pub_type: 杂志文章


    authors: Barnum SR,Ames RS,Maycox PR,Hadingham SJ,Meakin J,Harrison D,Parsons AA

    更新日期:2002-04-15 00:00:00

  • Altered immune response to CNS viral infection in mice with a conditional knock-down of macrophage-lineage cells.

    abstract::Neuroadapted Sindbis Virus (NSV) is a neuronotropic virus that causes hindlimb paralysis in susceptible mice and rats. The authors and others have demonstrated that though death of infected motor neurons occurs, bystander death of uninfected neurons also occurs and both contribute to the paralysis that ensues followin...


    pub_type: 杂志文章


    authors: Carmen J,Gowing G,Julien JP,Kerr D

    更新日期:2006-08-01 00:00:00

  • Distinct effects of bFGF and PDGF on oligodendrocyte progenitor cells.

    abstract::We have compared the effects of platelet-derived (PDGF) and basic fibroblast (bFGF) growth factors on the shape, migration, and differentiation of oligodendrocyte progenitor cells, the precursors of myelin-forming cells in the CNS. In the presence of bFGF, oligodendrocyte progenitors purified from rat neonatal brain c...


    pub_type: 杂志文章


    authors: McKinnon RD,Smith C,Behar T,Smith T,Dubois-Dalcq M

    更新日期:1993-03-01 00:00:00

  • Differentiation of induced pluripotent stem cells into functional oligodendrocytes.

    abstract::The technology to generate autologous pluripotent stem cells (iPS cells) from almost any somatic cell type has brought various cell replacement therapies within clinical research. Besides the challenge to optimize iPS protocols to appropriate safety and GMP levels, procedures need to be developed to differentiate iPS ...


    pub_type: 杂志文章


    authors: Czepiel M,Balasubramaniyan V,Schaafsma W,Stancic M,Mikkers H,Huisman C,Boddeke E,Copray S

    更新日期:2011-06-01 00:00:00

  • Motile membrane protrusions regulate cell-cell adhesion and migration of olfactory ensheathing glia.

    abstract::Olfactory ensheathing cells (OECs) are candidates for therapeutic approaches for neural regeneration due to their ability to assist axon regrowth in central nervous system lesion models. However, little is understood about the processes and mechanisms underlying migration of these cells. We report here that novel lame...


    pub_type: 杂志文章


    authors: Windus LC,Claxton C,Allen CL,Key B,St John JA

    更新日期:2007-12-01 00:00:00

  • Increased syndecan expression by pleiotrophin and FGF receptor-expressing astrocytes in injured brain tissue.

    abstract::Syndecan-1, -2, -3, and -4 are heparan sulfate proteoglycans that are differentially expressed during development and wound repair. To determine whether syndecans are also involved in brain injury, we examined the expression of syndecan core proteins genes in cryo-injured mouse brain, using in situ hybridization. All ...


    pub_type: 杂志文章


    authors: Iseki K,Hagino S,Mori T,Zhang Y,Yokoya S,Takaki H,Tase C,Murakawa M,Wanaka A

    更新日期:2002-07-01 00:00:00

  • Contrasting effects of mitogenic growth factors on myelination in neuron-oligodendrocyte co-cultures.

    abstract::Mitogenic growth factors play an important role in the initial stages of oligodendrocyte development, but their roles in the process of myelination itself remain less well defined. In order to study directly the effects of different growth factors on myelination, we used a purified in vitro co-culture system with dors...


    pub_type: 杂志文章


    authors: Wang Z,Colognato H,Ffrench-Constant C

    更新日期:2007-04-01 00:00:00

  • Enhanced connexin 43 immunoreactivity in penumbral areas in the human brain following ischemia.

    abstract::Astrocytes support neurons not only physically but also chemically by secreting neurotrophic factors and energy substrates. Moreover, astrocytes establish a glial network and communicate through gap junctions in the brain. Connexin 43 (Cx43) is one of major component proteins in astrocytic gap junctions. Heterozygote ...


    pub_type: 杂志文章


    authors: Nakase T,Yoshida Y,Nagata K

    更新日期:2006-10-01 00:00:00

  • A DAP12-dependent signal promotes pro-inflammatory polarization in microglia following nerve injury and exacerbates degeneration of injured neurons.

    abstract::Under pathological conditions, activated microglia play paradoxical roles and could have neurotoxic or neuroprotective effects. However, the signal determining how activated microglia affects the fate of neuronal cells remains largely unknown. Here we demonstrate that DNAX-activating protein of 12 kDa (DAP12), a trans...


    pub_type: 杂志文章


    authors: Kobayashi M,Konishi H,Takai T,Kiyama H

    更新日期:2015-06-01 00:00:00

  • Loss of galectin-3 decreases the number of immune cells in the subventricular zone and restores proliferation in a viral model of multiple sclerosis.

    abstract::Multiple sclerosis (MS) frequently starts near the lateral ventricles, which are lined by subventricular zone (SVZ) progenitor cells that can migrate to lesions and contribute to repair. Because MS-induced inflammation may decrease SVZ proliferation and thus limit repair, we studied the role of galectin-3 (Gal-3), a p...


    pub_type: 杂志文章


    authors: James RE,Hillis J,Adorján I,Gration B,Mundim MV,Iqbal AJ,Majumdar MM,Yates RL,Richards MM,Goings GE,DeLuca GC,Greaves DR,Miller SD,Szele FG

    更新日期:2016-01-01 00:00:00

  • Microglia and macrophages differentially modulate cell death after brain injury caused by oxygen-glucose deprivation in organotypic brain slices.

    abstract::Macrophage can adopt several phenotypes, process call polarization, which is crucial for shaping inflammatory responses to injury. It is not known if microglia, a resident brain macrophage population, polarizes in a similar way, and whether specific microglial phenotypes modulate cell death in response to brain injury...


    pub_type: 杂志文章


    authors: Girard S,Brough D,Lopez-Castejon G,Giles J,Rothwell NJ,Allan SM

    更新日期:2013-05-01 00:00:00

  • Homotypic cell contact-dependent inhibition of astrocyte proliferation.

    abstract::The normal adult vertebrate nervous system is a relative quiescent tissue in terms of cell proliferation. However, astrocytes in many regions of the central nervous system (CNS) retain the capacity to undergo cell division. To examine the mechanisms that regulate the proliferation of astrocytes in the CNS we have util...


    pub_type: 杂志文章


    authors: Nakatsuji Y,Miller RH

    更新日期:1998-04-01 00:00:00

  • Alterations in metabolism and gap junction expression may determine the role of astrocytes as "good samaritans" or executioners.

    abstract::Our knowledge of astroglia and their physiological and pathophysiological role(s) in the central nervous system (CNS) has grown during the past decade, revealing a complex picture. It is becoming increasingly clear that glia play a significant role in the homeostasis and function of the CNS and that neurons should no ...


    pub_type: 杂志文章


    authors: Farahani R,Pina-Benabou MH,Kyrozis A,Siddiq A,Barradas PC,Chiu FC,Cavalcante LA,Lai JC,Stanton PK,Rozental R

    更新日期:2005-06-01 00:00:00

  • Migration of Schwann cells from the distal stump of the sciatic nerve 1 week after transection: the effects of insulin and cytosine arabinoside.

    abstract::We have examined the migratory capacity of Schwann cells from the distal stump of a 1-week transected sciatic nerve of adult rat for a distance of 10 mm. The distal stump was introduced into the open end of a silicone chamber packed with artificial fibrin sponge (Gelaspon) soaked in phosphate-buffered saline (control ...


    pub_type: 杂志文章


    authors: Dubový P,Svízenská I

    更新日期:1992-01-01 00:00:00

  • Phagocytosis of neuronal debris by microglia is associated with neuronal damage in multiple sclerosis.

    abstract::Neuroaxonal degeneration is a pathological hallmark of multiple sclerosis (MS) contributing to irreversible neurological disability. Pathological mechanisms leading to axonal damage include autoimmunity to neuronal antigens. In actively demyelinating lesions, myelin is phagocytosed by microglia and blood-borne macroph...


    pub_type: 杂志文章


    authors: Huizinga R,van der Star BJ,Kipp M,Jong R,Gerritsen W,Clarner T,Puentes F,Dijkstra CD,van der Valk P,Amor S

    更新日期:2012-03-01 00:00:00

  • Single-cell transcriptomic profiling of satellite glial cells in stellate ganglia reveals developmental and functional axial dynamics.

    abstract::Stellate ganglion neurons, important mediators of cardiopulmonary neurotransmission, are surrounded by satellite glial cells (SGCs), which are essential for the function, maintenance, and development of neurons. However, it remains unknown whether SGCs in adult sympathetic ganglia exhibit any functional diversity, and...


    pub_type: 杂志文章


    authors: van Weperen VYH,Littman RJ,Arneson DV,Contreras J,Yang X,Ajijola OA

    更新日期:2021-01-12 00:00:00

  • Calcium waves between astrocytes from Cx43 knockout mice.

    abstract::Gap junctions are regarded as the primary pathway underlying propagation of Ca2+ waves between astrocytes, although signaling through extracellular space may also contribute. Results obtained from astrocytes cultured from sibling Cx43 knockout (KO) and wild-type (WT) mice in six litters showed that Ca2+ waves propagat...


    pub_type: 杂志文章


    authors: Scemes E,Dermietzel R,Spray DC

    更新日期:1998-09-01 00:00:00

  • Myelination, oligodendrocytes, and serious mental illness.

    abstract::Historically, the human brain has been conceptually segregated from the periphery and further dichotomized into gray matter (GM) and white matter (WM) based on the whitish appearance of the exceptionally high lipid content of the myelin sheaths encasing neuronal axons. These simplistic dichotomies were unfortunately e...


    pub_type: 杂志文章,评审


    authors: Haroutunian V,Katsel P,Roussos P,Davis KL,Altshuler LL,Bartzokis G

    更新日期:2014-11-01 00:00:00

  • Bystander killing of neurons by cytotoxic T cells specific for a glial antigen.

    abstract::To explore pathways to neuron loss in inflammatory diseases, transgenic mice expressing beta-galactosidase (beta-gal) in either astrocytes or photoreceptor cells, or both, were inoculated with activated, beta-gal-specific cytotoxic CD8 T lymphocytes (CTLs). beta-gal-positive astrocytes in brain were rapidly attacked, ...


    pub_type: 杂志文章


    authors: McPherson SW,Heuss ND,Roehrich H,Gregerson DS

    更新日期:2006-04-01 00:00:00

  • Activation of interferon signaling pathways in spinal cord astrocytes from an ALS mouse model.

    abstract::Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disorder affecting predominantly motor neurons. Recent studies suggest that the disease progression of ALS is non-cell-autonomous, although the interaction between neurons and glial cells in different disease stages is not entirely clear. Here, we ...


    pub_type: 杂志文章


    authors: Wang R,Yang B,Zhang D

    更新日期:2011-06-01 00:00:00

  • Expression of the B7-related molecule ICOSL by human glioma cells in vitro and in vivo.

    abstract::Human glioblastoma is a highly lethal tumor known for its capability of interfering with effective antitumor immune responses. Costimulatory signals are of critical relevance in both the inductive and effector phases of immune responses. Inducible costimulator-ligand (ICOSL), a member of the B7 family of costimulatory...


    pub_type: 杂志文章


    authors: Schreiner B,Wischhusen J,Mitsdoerffer M,Schneider D,Bornemann A,Melms A,Tolosa E,Weller M,Wiendl H

    更新日期:2003-12-01 00:00:00

  • Lesion-induced changes of electrophysiological properties in astrocytes of the rat dentate gyrus.

    abstract::Reorganization of the adult dentate gyrus following unilateral entorhinal cortex lesion (ECL) is a well-established model for studying mechanisms of trauma-induced neuronal plasticity. The lesion induces deafferentiation of the outer molecular layer, which is accompanied by a strong astroglial reaction. This glial res...


    pub_type: 杂志文章


    authors: Schröder W,Hager G,Kouprijanova E,Weber M,Schmitt AB,Seifert G,Steinhäuser C

    更新日期:1999-11-01 00:00:00

  • The microglial reaction signature revealed by RNAseq from individual mice.

    abstract::Microglial cells have a double life as the immune cells of the brain in times of stress but have also specific physiological functions in homeostatic conditions. In pathological contexts, microglia undergo a phenotypic switch called "reaction" that promotes the initiation and the propagation of neuro-inflammation. Rea...


    pub_type: 杂志文章


    authors: Hirbec H,Marmai C,Duroux-Richard I,Roubert C,Esclangon A,Croze S,Lachuer J,Peyroutou R,Rassendren F

    更新日期:2018-05-01 00:00:00

  • Extensive and long-lasting changes of glial cells following transection of the postcommissural fornix in the adult rat.

    abstract::Injury to the adult brain results in the formation of a glial scar that involves both activated astroglia and microglia/macrophages. Although reactive gliosis has been linked to failure of axonal regeneration in the adult mammalian CNS, the spatio-temporal pattern of the postlesion responses in morphology and distribu...


    pub_type: 杂志文章


    authors: Stichel CC,Müller HW

    更新日期:1994-02-01 00:00:00

  • Cell therapy for spinal cord injury with olfactory ensheathing glia cells (OECs).

    abstract::The prospects of achieving regeneration in the central nervous system (CNS) have changed, as most recent findings indicate that several species, including humans, can produce neurons in adulthood. Studies targeting this property may be considered as potential therapeutic strategies to respond to injury or the effects ...


    pub_type: 杂志文章,评审


    authors: Gómez RM,Sánchez MY,Portela-Lomba M,Ghotme K,Barreto GE,Sierra J,Moreno-Flores MT

    更新日期:2018-07-01 00:00:00

  • Cathepsin S release from primary cultured microglia is regulated by the P2X7 receptor.

    abstract::Microglia respond rapidly to injury, increasing their synthesis and release of inflammatory mediators, many of which contribute to the maintenance of persistent pain following CNS or PNS injury. We have recently shown that the lysosomal cysteine protease Cathepsin S (CatS) expressed by spinal microglia is vital for th...


    pub_type: 杂志文章


    authors: Clark AK,Wodarski R,Guida F,Sasso O,Malcangio M

    更新日期:2010-11-01 00:00:00

  • Multidrug resistance-associated protein 4 expression in ammonia-treated cultured rat astrocytes and cerebral cortex of cirrhotic patients with hepatic encephalopathy.

    abstract::Hepatic encephalopathy (HE) is a neuropsychiatric syndrome frequently accompanying liver cirrhosis and reflects the clinical manifestation of a low grade cerebral edema associated with cerebral oxidative/nitrosative stress. The multidrug resistance-associated protein (Mrp) 4 is an export pump which transports metaboli...


    pub_type: 杂志文章


    authors: Jördens MS,Keitel V,Karababa A,Zemtsova I,Bronger H,Häussinger D,Görg B

    更新日期:2015-11-01 00:00:00

  • Inducible gene expression in GFAP+ progenitor cells of the SGZ and the dorsal wall of the SVZ-A novel tool to manipulate and trace adult neurogenesis.

    abstract::In the adult mammalian brain, neurogenesis originates from astrocyte-like stem cells. We generated a transgenic mouse line in which the tetracycline dependent transactivator (tTA) is expressed under the control of the murine GFAP promoter. In this mouse line, inducible gene expression targets virtually all GFAP-expres...


    pub_type: 杂志文章


    authors: Weber T,Baier V,Pauly R,Sahay A,Baur M,Herrmann E,Ciccolini F,Hen R,Kronenberg G,Bartsch D

    更新日期:2011-04-01 00:00:00

  • Membrane-initiated effects of progesterone on calcium dependent signaling and activation of VEGF gene expression in retinal glial cells.

    abstract::Neurosteroids, such as progesterone, influence central nervous system development and function by regulating a broad spectrum of physiological processes. Here, we investigated membrane-initiated actions of progesterone in the retina and identified the membrane-associated progesterone receptor component 1 (PGRMC1). We ...


    pub_type: 杂志文章


    authors: Swiatek-De Lange M,Stampfl A,Hauck SM,Zischka H,Gloeckner CJ,Deeg CA,Ueffing M

    更新日期:2007-08-01 00:00:00

  • T- and B-cell responses to myelin oligodendrocyte glycoprotein in experimental autoimmune encephalomyelitis and multiple sclerosis.

    abstract::The identification of myelin oligodendrocyte glycoprotein (MOG) as a target for autoantibody-mediated demyelination in experimental autoimmune encephalomyelitis (EAE) resulted in the re-evaluation of the role of B cell responses to myelin autoantigens in the immunopathogenesis of multiple sclerosis. MOG is a central n...


    pub_type: 杂志文章,评审


    authors: Iglesias A,Bauer J,Litzenburger T,Schubart A,Linington C

    更新日期:2001-11-01 00:00:00