Expression of N-methyl D-aspartate receptor subunits in amoeboid microglia mediates production of nitric oxide via NF-κB signaling pathway and oligodendrocyte cell death in hypoxic postnatal rats.


:The present study was focused on identifying the expression of N-methyl D-aspartate receptor (NMDAR) subunits on activated microglia and to determine their role in the pathogenesis of periventricular white matter damage (PWMD) in neonatal rats following hypoxia. One day old wistar rats were subjected to hypoxia (5% O(2) ; 95% N(2) ) and the mRNA and protein expression of NMDAR subunits (NR1, NR2A-D, and NR3A) in the periventricular white matter (PWM) was determined at different time points (3,24 h, 3, 7, and 14 days) following hypoxic exposure. Immunoexpression of NR1 and NR2A-D was localized in amoeboid microglial cells (AMC) suggesting the presence of functional NMDARs in them. The expression of NMDAR in primary microglial cultures was ascertained by RT-PCR analysis and double immunofluorescence studies. The functionality of the microglial NMDAR in cultured microglial cells was examined by monitoring calcium movements in cells with fura-2. In primary microglial cultures, hypoxia induced the nuclear translocation of NF-κB which was suppressed by administration of MK801, an NMDAR antagonist. MK801 also down regulated the hypoxia-induced expression of tumor necrosis factor-α, interleukin-1β, inducible nitric oxide synthase (iNOS), and nitric oxide (NO) production by microglia which may be mediated by the NF-κB signaling pathway. NO produced by microglia is known to cause death of oligodendrocytes in the developing PWM. In this connection, pharmacological agents such as MK801, BAY (NF-κB inhibitor), and 1400w (iNOS inhibitor) proved to be beneficial since they reduced the hypoxia-induced iNOS expression, NO production, and a corresponding reduction in the death of oligodendrocytes following hypoxia.






Murugan M,Sivakumar V,Lu J,Ling EA,Kaur C




Has Abstract


2011-04-01 00:00:00














  • Purinergic P2Y receptors on astrocytes are directly coupled to phospholipase A2.

    abstract::ATP stimulates arachidonic acid mobilization and eicosanoid production in cultured astrocytes via P2Y-purinergic receptors. To assist in determining the mechanism of phospholipase A2 activation and the role of calcium in eicosanoid production, cultures were pretreated with pertussis toxin (PTx). ATP-evoked eicosanoid ...


    pub_type: 杂志文章


    authors: Bruner G,Murphy S

    更新日期:1993-03-01 00:00:00

  • Amyloid beta deregulates astroglial mGluR5-mediated calcium signaling via calcineurin and Nf-kB.

    abstract::The amyloid hypothesis of Alzheimer's disease (AD) suggests that soluble amyloid β (Aβ) is an initiator of a cascade of events eventually leading to neurodegeneration. Recently, we reported that Aβ deranged Ca(2+) homeostasis specifically in hippocampal astrocytes by targeting key elements of Ca(2+) signaling, such as...


    pub_type: 杂志文章


    authors: Lim D,Iyer A,Ronco V,Grolla AA,Canonico PL,Aronica E,Genazzani AA

    更新日期:2013-07-01 00:00:00

  • Nerve growth factor attenuates proliferation of astrocytes via the p75 neurotrophin receptor.

    abstract::The p75 neurotrophin receptor has been implicated in the regulation of multiple cellular functions that differ depending on the cell context. We have observed that p75(NTR) is strongly induced on astrocytes as well as neurons in the hippocampal CA3 region after seizures; however, the function of this receptor on these...


    pub_type: 杂志文章


    authors: Cragnolini AB,Huang Y,Gokina P,Friedman WJ

    更新日期:2009-10-01 00:00:00

  • Hyperpolarization-activated ion currents in cultured rat cortical and spinal cord astrocytes.

    abstract::Hyperpolarization-activated currents were recorded from rat brain cortical and spinal cord astrocytes maintained in culture. Spinal cord astrocytes expressed primarily an inward rectifier potassium current characterized by time-dependent inactivation, a strong dependence on extracellular Na+ and insensitivity to intra...


    pub_type: 杂志文章


    authors: Guatteo E,Stanness KA,Janigro D

    更新日期:1996-03-01 00:00:00

  • Type I interferon pathway in CNS homeostasis and neurological disorders.

    abstract::Type I interferons (IFNs), IFN-α and IFN-β, represent the major effector cytokines of the host immune response against viruses and other intracellular pathogens. These cytokines are produced via activation of numerous pattern recognition receptors, including the Toll-like receptor signaling network, retinoic acid-indu...


    pub_type: 杂志文章,评审


    authors: Blank T,Prinz M

    更新日期:2017-09-01 00:00:00

  • Transplantation of Schwann cells and/or olfactory ensheathing glia into the contused spinal cord: Survival, migration, axon association, and functional recovery.

    abstract::Schwann cells (SCs) and olfactory ensheathing glia (OEG) have shown promise for spinal cord injury repair. We sought their in vivo identification following transplantation into the contused adult rat spinal cord at 1 week post-injury by: (i) DNA in situ hybridization (ISH) with a Y-chromosome specific probe to identif...


    pub_type: 杂志文章


    authors: Pearse DD,Sanchez AR,Pereira FC,Andrade CM,Puzis R,Pressman Y,Golden K,Kitay BM,Blits B,Wood PM,Bunge MB

    更新日期:2007-07-01 00:00:00

  • Glial cells promote dendrite formation and the reception of synaptic input in Purkinje cells from postnatal mice.

    abstract::Previous studies suggest that glial cells contribute to synaptogenesis in specific neurons from the postnatal CNS. Here, we studied whether this is true for Purkinje cells (PCs), which represent a unique neuronal cell type due to their large size, massive synaptic input, and high vulnerability. Using new glia-free cul...


    pub_type: 杂志文章


    authors: Buard I,Steinmetz CC,Claudepierre T,Pfrieger FW

    更新日期:2010-04-01 00:00:00

  • Adaptive phenotype of microglial cells during the normal postnatal development of the somatosensory "Barrel" cortex.

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    pub_type: 杂志文章


    authors: Arnoux I,Hoshiko M,Mandavy L,Avignone E,Yamamoto N,Audinat E

    更新日期:2013-10-01 00:00:00

  • Microglia modulate respiratory rhythm generation and autoresuscitation.

    abstract::Inflammation has been linked to the induction of apneas and Sudden Infant Death Syndrome, whereas proinflammatory mediators inhibit breathing when applied peripherally or directly into the CNS. Considering that peripheral inflammation can activate microglia in the CNS and that this cell type can directly release all p...


    pub_type: 杂志文章


    authors: Lorea-Hernández JJ,Morales T,Rivera-Angulo AJ,Alcantara-Gonzalez D,Peña-Ortega F

    更新日期:2016-04-01 00:00:00

  • Rehabilitative therapies differentially alter proliferation and survival of glial cell populations in the perilesional zone of cortical infarcts.

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    pub_type: 杂志文章


    authors: Keiner S,Wurm F,Kunze A,Witte OW,Redecker C

    更新日期:2008-04-01 00:00:00

  • The role of chromatin remodeling complexes in Schwann cell development.

    abstract::Schwann cells develop from neural crest cells in an ordered series of events and give rise to myelinating and nonmyelinating subtypes. In their mature state, myelinating Schwann cells produce myelin sheaths that provide trophic support to axons and allow saltatory conduction in the vertebrate peripheral nervous system...


    pub_type: 杂志文章


    authors: Fröb F,Wegner M

    更新日期:2020-08-01 00:00:00

  • Calcium-mobilizing and electrophysiological effects of bradykinin on cortical astrocyte subtypes in culture.

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    pub_type: 杂志文章


    authors: Stephens GJ,Cholewinski AJ,Wilkin GP,Djamgoz MB

    更新日期:1993-12-01 00:00:00

  • DNA sensors are expressed in astrocytes and microglia in vitro and are upregulated during gliosis in neurodegenerative disease.

    abstract::The detection of nucleic acids by the innate immune system is an essential host response during viral infection. In recent years, a number of immune sensors capable of recognizing cytosolic DNA have been identified and include the PYHIN family members AIM2, IFI16, and p204 as well as the enzyme, cGAS. Activation of th...


    pub_type: 杂志文章


    authors: Cox DJ,Field RH,Williams DG,Baran M,Bowie AG,Cunningham C,Dunne A

    更新日期:2015-05-01 00:00:00

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    pub_type: 杂志文章


    authors: Lyons PD,Benveniste EN

    更新日期:1998-02-01 00:00:00

  • Expression of the Krüppel-type zinc finger protein rKr2 in the developing nervous system.

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    pub_type: 杂志文章


    authors: Lovas G,Li W,Pott U,Verga T,Hudson LD

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  • Stimulation of transient receptor potential vanilloid 4 channel suppresses abnormal activation of microglia induced by lipopolysaccharide.

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    pub_type: 杂志文章


    authors: Konno M,Shirakawa H,Iida S,Sakimoto S,Matsutani I,Miyake T,Kageyama K,Nakagawa T,Shibasaki K,Kaneko S

    更新日期:2012-05-01 00:00:00

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    abstract::Microglia, the innate immune cells of the brain, have recently been removed from the position of mere sentinels and promoted to the role of active sculptors of developing circuits and cells. Alongside their functions in normal brain development, microglia coordinate sexual differentiation of the brain, a set of proces...


    pub_type: 杂志文章,评审


    authors: VanRyzin JW,Marquardt AE,Pickett LA,McCarthy MM

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    pub_type: 杂志文章


    authors: Salm AK,McCarthy KD

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  • Expression of the complement C3a and C5a receptors after permanent focal ischemia: An alternative interpretation.

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    pub_type: 杂志文章


    authors: Barnum SR,Ames RS,Maycox PR,Hadingham SJ,Meakin J,Harrison D,Parsons AA

    更新日期:2002-04-15 00:00:00

  • A DAP12-dependent signal promotes pro-inflammatory polarization in microglia following nerve injury and exacerbates degeneration of injured neurons.

    abstract::Under pathological conditions, activated microglia play paradoxical roles and could have neurotoxic or neuroprotective effects. However, the signal determining how activated microglia affects the fate of neuronal cells remains largely unknown. Here we demonstrate that DNAX-activating protein of 12 kDa (DAP12), a trans...


    pub_type: 杂志文章


    authors: Kobayashi M,Konishi H,Takai T,Kiyama H

    更新日期:2015-06-01 00:00:00

  • Sphingosine-1-phosphate is released by cerebellar astrocytes in response to bFGF and induces astrocyte proliferation through Gi-protein-coupled receptors.

    abstract::The mitogenic role of sphingosine-1-phosphate (S1P) and its involvement in basic fibroblast growth factor (bFGF)-induced proliferation were examined in primary cultures of cerebellar astrocytes. Exposure to bFGF resulted in a rapid increase of extracellular S1P formation, bFGF inducing astrocytes to release S1P, but n...


    pub_type: 杂志文章


    authors: Bassi R,Anelli V,Giussani P,Tettamanti G,Viani P,Riboni L

    更新日期:2006-04-15 00:00:00

  • Activating transcription factor 6α deficiency exacerbates oligodendrocyte death and myelin damage in immune-mediated demyelinating diseases.

    abstract::Endoplasmic reticulum (ER) stress and the unfolded protein response (UPR) play a critical role in immune-mediated demyelinating diseases, including multiple sclerosis (MS) and its animal model experimental autoimmune encephalomyelitis (EAE), by regulating the viability of oligodendrocytes. Our previous studies show th...


    pub_type: 杂志文章


    authors: Stone S,Wu S,Jamison S,Durose W,Pallais JP,Lin W

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  • Sodium channels contribute to microglia/macrophage activation and function in EAE and MS.

    abstract::Loss of axons is a major contributor to nonremitting deficits in the inflammatory demyelinating disease multiple sclerosis (MS). Based on biophysical studies showing that activity of axonal sodium channels can trigger axonal degeneration, recent studies have tested sodium channel-blocking drugs in experimental autoimm...


    pub_type: 杂志文章


    authors: Craner MJ,Damarjian TG,Liu S,Hains BC,Lo AC,Black JA,Newcombe J,Cuzner ML,Waxman SG

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  • Disruption of dopaminergic transmission remodels tripartite synapse morphology and astrocytic calcium activity within substantia nigra pars reticulata.

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    pub_type: 杂志文章


    authors: Bosson A,Boisseau S,Buisson A,Savasta M,Albrieux M

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  • Lesion-induced changes of electrophysiological properties in astrocytes of the rat dentate gyrus.

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    pub_type: 杂志文章


    authors: Schröder W,Hager G,Kouprijanova E,Weber M,Schmitt AB,Seifert G,Steinhäuser C

    更新日期:1999-11-01 00:00:00

  • Gene expression profiling reveals a conserved microglia signature in larval zebrafish.

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    pub_type: 杂志文章


    authors: Mazzolini J,Le Clerc S,Morisse G,Coulonges C,Kuil LE,van Ham TJ,Zagury JF,Sieger D

    更新日期:2020-02-01 00:00:00

  • Structural organization of the perivascular astrocyte endfeet and their relationship with the endothelial glucose transporter: a confocal microscopy study.

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    pub_type: 杂志文章


    authors: Kacem K,Lacombe P,Seylaz J,Bonvento G

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  • Potassium channel Kir4.1 macromolecular complex in retinal glial cells.

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    pub_type: 杂志文章


    authors: Connors NC,Kofuji P

    更新日期:2006-01-15 00:00:00

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    abstract::Two membrane-localized transporter proteins (GLYT1 and GLYT2) are responsible for removal of extracellular glycine in the mammalian CNS. Whereas GLYT1 seems to be expressed mainly in glial cells, GLYT2 is neuronal. The highest concentrations of both transporters are found in glycinergic areas of the nervous system. Th...


    pub_type: 杂志文章


    authors: Zafra F,Poyatos I,Gimenez C

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  • Downregulation of miR-7116-5p in microglia by MPP+ sensitizes TNF-α production to induce dopaminergic neuron damage.

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    pub_type: 杂志文章


    authors: He Q,Wang Q,Yuan C,Wang Y

    更新日期:2017-08-01 00:00:00