Brain glucose transporter (Glut3) haploinsufficiency does not impair mouse brain glucose uptake.

Abstract:

:Mouse brain expresses three principal glucose transporters. Glut1 is an endothelial marker and is the principal glucose transporter of the blood-brain barrier. Glut3 and Glut6 are expressed in glial cells and neural cells. A mouse line with a null allele for Glut3 has been developed. The Glut3(-/-) genotype is intrauterine lethal by 7days post-coitis, but the heterozygous (Glut3(+/-)) littermate survives, exhibiting rapid post-natal weight gain, but no seizures or other behavioral aberrations. At 12weeks of age, brain uptake of tail vein-injected ((3))H-2-deoxy glucose in Glut3(+/-) mice was not different from Glut3(+/+) littermates, despite 50% less Glut3 protein expression in the brain. The brain uptake of injected ((18))F-2-fluoro-2-deoxy glucose was similarly not different from Glut3(+/-) littermates in the total amount, time course, or brain imaging in the Glut3(+/-) mice. Glut1 and Glut6 protein expressions evaluated by immunoblots were not affected by the diminished Glut3 expression in the Glut3(+/-) mice. We conclude that a 50% decrease in Glut3 is not limiting for the uptake of glucose into the mouse brain, since Glut3 haploinsufficiency does not impair brain glucose uptake or utilization.

journal_name

Brain Res

journal_title

Brain research

authors

Stuart CA,Ross IR,Howell ME,McCurry MP,Wood TG,Ceci JD,Kennel SJ,Wall J

doi

10.1016/j.brainres.2011.02.014

subject

Has Abstract

pub_date

2011-04-12 00:00:00

pages

15-22

eissn

0006-8993

issn

1872-6240

pii

S0006-8993(11)00288-5

journal_volume

1384

pub_type

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