Abstract:
:Fibroblastic 3T3 and endocrine RIN cells were genetically modified by infection with a recombinant retrovirus encoding the form I of human tyrosine hydroxylase (TH) and selection in tyrosine-free medium. These cells were grafted to rats unilaterally lesioned with 6-hydroxy-dopamine. Both cell types survived implantation into the striatum, expressed TH immunoreactivity, and as assessed by microdialysis 8-9 days after implantation, secreted high amounts of DOPA and/or dopamine into the surrounding host striatum. The modified 3T3 cells secreted large amounts of DOPA that was efficiently decarboxylated to dopamine by the host striatal tissue; the newly synthesized dopamine was stored only to a limited extent in the denervated striatum. The modified RIN cells synthesized dopamine that was stored intracellularly and released in a regulated fashion. The grafted DOPA-secreting cells produced 4-5 times higher extracellular dopamine levels than the dopamine-secreting cells, and they were more efficient in reducing apomorphine-induced rotation. No effect was observed with either cell type on amphetamine-induced turning behavior.
journal_name
Neuronjournal_title
Neuronauthors
Horellou P,Brundin P,Kalén P,Mallet J,Björklund Adoi
10.1016/0896-6273(90)90078-tsubject
Has Abstractpub_date
1990-10-01 00:00:00pages
393-402issue
4eissn
0896-6273issn
1097-4199pii
0896-6273(90)90078-Tjournal_volume
5pub_type
杂志文章相关文献
NEURON文献大全abstract::T-type calcium channels are essential contributors to the transmission of nociceptive signals in the primary afferent pain pathway. Here, we show that T-type calcium channels are ubiquitinated by WWP1, a plasma-membrane-associated ubiquitin ligase that binds to the intracellular domain III-IV linker region of the Cav3...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2014.07.036
更新日期:2014-09-03 00:00:00
abstract::Nicotinic acetylcholine receptors (AChRs) immunoaffinity-purified from brains are composed of only two kinds of subunits rather than the four kinds present in muscle-type AChRs. Here we report the N-terminal protein sequences of the structural subunits of AChRs from rat and chicken brains and the cloning of full-lengt...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(88)90145-6
更新日期:1988-05-01 00:00:00
abstract::Light plays a pivotal role in the regulation of affective behaviors. However, the precise circuits that mediate the impact of light on depressive-like behaviors are not well understood. Here, we show that light influences depressive-like behaviors through a disynaptic circuit linking the retina and the lateral habenul...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2019.01.037
更新日期:2019-04-03 00:00:00
abstract::The retinal pigment epithelium (RPE) plays an essential role in the normal development of the underlying neural retina, but the mechanisms by which this regulation occurs are largely unknown. Ca2+ transients, induced by the neurotransmitter ATP acting on purinergic receptors, both increase proliferation and stimulate ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2005.04.024
更新日期:2005-06-02 00:00:00
abstract::Movements are universally, sometimes frustratingly, variable. When such variability causes error, we typically assume that something went wrong during the movement. The same assumption is made by recent and influential models of motor control. These posit that the principal limit on repeatable performance is neuromusc...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2006.10.034
更新日期:2006-12-21 00:00:00
abstract::During synaptic development, proteins aggregate at specialized pre- and postsynaptic structures. Mechanisms that mediate protein clustering at these sites remain unknown. To investigate this process, we analyzed synaptic targeting of a postsynaptic density protein, PSD-95, by expressing green fluorescent protein- (GFP...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)80705-9
更新日期:1999-03-01 00:00:00
abstract::Distinct brain regions, reproducible from one person to the next, are specialized for processing different kinds of human expertise, such as face recognition and reading. Here, we explore the relationship between age of learning, learning ability, and specialized brain structures. Specifically, we ask whether the exis...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2011.12.022
更新日期:2012-02-09 00:00:00
abstract::An axon is thought to faithfully conduct action potentials to its terminals. However, many features of the axon and axon terminals, especially at inhibitory synapses, remain unknown. By directly recording from the axon and terminal of a cultured cerebellar Purkinje cell (PC), we demonstrate that low membrane excitabil...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2015.02.013
更新日期:2015-03-18 00:00:00
abstract::The study of interneuron diversity in the spinal cord is complex and needs new models that can accelerate discovery. In this issue, Hoang et al. (2018) use ESC-derived neurons to create simplified microcircuits to study spinal interneuron diversification, connectivity, and function. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2018.09.034
更新日期:2018-10-10 00:00:00
abstract::X-ray crystallography has made considerable recent progress in providing static structures of ion channels. Here we describe a complementary method-systematic fluorescence scanning-that reveals the structural dynamics of a channel. Local protein motion was measured from changes in the fluorescent intensity of a fluoro...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)00068-4
更新日期:2000-09-01 00:00:00
abstract::Simple decisions arise from the evaluation of sensory evidence. But decisions are determined by more than just evidence. Individuals establish internal decision criteria that influence how they respond. Where or how decision criteria are established in the brain remains poorly understood. Here, we show that neuronal a...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2017.12.006
更新日期:2018-01-03 00:00:00
abstract::The serine hydrolase α/β-hydrolase domain 6 (ABHD6) hydrolyzes the most abundant endocannabinoid (eCB) in the brain, 2-arachidonoylglycerol (2-AG), and controls its availability at cannabinoid receptors. We show that ABHD6 inhibition decreases pentylenetetrazole (PTZ)-induced generalized tonic-clonic and myoclonic sei...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2014.06.030
更新日期:2014-07-16 00:00:00
abstract::Two papers in the current issue of Neuron (Izuma et al. and Zink et al.) report that activity in specific regions of the brain, especially the striatum, reflects a common signal of reward in both the economic (e.g., money) and social (e.g., praise and status) domains. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2008.04.005
更新日期:2008-04-24 00:00:00
abstract::According to economic theories, preference for one item over others reveals its rank value on a common scale. Previous studies identified brain regions encoding such values. Here we verify that these regions can valuate various categories of objects and further test whether they still express preferences when attentio...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2009.09.040
更新日期:2009-11-12 00:00:00
abstract::Degeneration of basal forebrain cholinergic neurons (BFCNs) contributes to cognitive dysfunction in Alzheimer's disease (AD) and Down's syndrome (DS). We used Ts65Dn and Ts1Cje mouse models of DS to show that the increased dose of the amyloid precursor protein gene, App, acts to markedly decrease NGF retrograde transp...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2006.05.022
更新日期:2006-07-06 00:00:00
abstract::Studies on postmortem brains from Parkinson's patients reveal elevated iron in the substantia nigra (SN). Selective cell death in this brain region is associated with oxidative stress, which may be exacerbated by the presence of excess iron. Whether iron plays a causative role in cell death, however, is controversial....
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(03)00126-0
更新日期:2003-03-27 00:00:00
abstract::Cortical development depends upon tightly controlled cell fate and cell survival decisions that generate a functional neuronal population, but the coordination of these two processes is poorly understood. Here we show that conditional removal of a key apical complex protein, Pals1, causes premature withdrawal from the...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2010.03.019
更新日期:2010-04-15 00:00:00
abstract::Improvement in motor skill performance is known to continue for at least 24 hr following training, yet the relative contributions of time spent awake and asleep are unknown. Here we provide evidence that a night of sleep results in a 20% increase in motor speed without loss of accuracy, while an equivalent period of t...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(02)00746-8
更新日期:2002-07-03 00:00:00
abstract::Locomotion is controlled by spinal networks that generate rhythm and coordinate left-right and flexor-extensor patterning. Defined populations of spinal interneurons have been linked to patterning circuits; however, neurons comprising the rhythm-generating kernel have remained elusive. Here, we identify an ipsilateral...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2013.08.015
更新日期:2013-11-20 00:00:00
abstract::Feedback is a ubiquitous anatomical feature of sensory processing in vertebrates. In this issue of Neuron, two papers (Boyd et al., 2012, and Markopoulos et al., 2012) analyze the features of feedback from olfactory cortex to olfactory bulb. ...
journal_title:Neuron
pub_type: 评论,杂志文章
doi:10.1016/j.neuron.2012.12.006
更新日期:2012-12-20 00:00:00
abstract::The calcium-activated small conductance potassium channel SK3 plays an essential role in the regulation of dopamine neuron activity patterns. Here we demonstrate that expression of a human disease-related SK3 mutation (hSK3Δ) in dopamine neurons of mice disrupts the balance between tonic and phasic dopamine neuron act...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2013.07.044
更新日期:2013-11-20 00:00:00
abstract::Synaptotagmin 7 (Syt7) was originally identified as a slow Ca2+ sensor for lysosome fusion, but its function at fast synapses is controversial. The paper by Luo and Südhof (2017) in this issue of Neuron shows that at the calyx of Held in the auditory brainstem Syt7 triggers asynchronous release during stimulus trains,...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2017.05.011
更新日期:2017-05-17 00:00:00
abstract::Small conductance Ca(2+)-activated K(+) (SK) channels and voltage-gated A-type Kv4 channels shape dendritic excitatory postsynaptic potentials (EPSPs) in hippocampal CA1 pyramidal neurons. Synaptically evoked Ca(2+) influx through N-methyl-D-aspartate receptors (NMDARs) activates spine SK channels, reducing EPSPs and ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2013.11.004
更新日期:2014-01-22 00:00:00
abstract::A new fluorescence method using the dye FM1-43 was used to examine exocytotic release from hippocampal synaptosomes. Nitric oxide caused a marked transient stimulation of vesicle release. Several structurally unrelated nitric oxide donors, sodium nitroprusside, S-nitroso-N-acetylpenicillamine, 3-morpholino-sydnonimine...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(94)90440-5
更新日期:1994-06-01 00:00:00
abstract::Recent studies indicate that long interspersed nuclear element-1 (L1) are mobilized in the genome of human neural progenitor cells and enhanced in Rett syndrome and ataxia telangiectasia. However, whether aberrant L1 retrotransposition occurs in mental disorders is unknown. Here, we report high L1 copy number in schiz...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2013.10.053
更新日期:2014-01-22 00:00:00
abstract::For efficient coding, sensory systems need to adapt to the distribution of signals to which they are exposed. In vision, natural scenes above and below the horizon differ in the distribution of chromatic and achromatic features. Consequently, many species differentially sample light in the sky and on the ground using ...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2013.09.030
更新日期:2013-12-04 00:00:00
abstract::The dopamine D3 receptor is expressed primarily in regions of the brain that are thought to influence motivation and motor functions. To specify in vivo D3 receptor function, we generated mutant mice lacking this receptor. Our analysis indicates that in a novel environment, D3 mutant mice are transiently more active t...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)80965-4
更新日期:1997-10-01 00:00:00
abstract::The synaptic vesicle protein synaptotagmin I binds Ca2+ and is required for efficient neurotransmitter release. Here, we measure the response time of the C2 domains of synaptotagmin to determine whether synaptotagmin is fast enough to function as a Ca2+ sensor for rapid exocytosis. We report that synaptotagmin is "tun...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/s0896-6273(00)80850-8
更新日期:1999-10-01 00:00:00
abstract::The chemokine Cxcl12 binds Cxcr4 and Cxcr7 receptors to control cell migration in multiple biological contexts, including brain development, leukocyte trafficking, and tumorigenesis. Both receptors are expressed in the CNS, but how they cooperate during migration has not been elucidated. Here, we used the migration of...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/j.neuron.2010.12.006
更新日期:2011-01-13 00:00:00
abstract::Vertebrate rod and cone photoreceptors hyperpolarize when illuminated. However, synaptic input from horizontal cells can depolarize cones and even elicit action potentials. Using the whole-cell tight-seal recording technique, we determined that, in solitary cones isolated from a lizard retina, action potentials can be...
journal_title:Neuron
pub_type: 杂志文章
doi:10.1016/0896-6273(88)90181-x
更新日期:1988-08-01 00:00:00