Abstract：
PURPOSE:To evaluate the use of 2-photon microscopy (TPM), which excites tissue autofluorescence, in detecting and calculating the grade of collagen corneal crosslinks, which are not visible through the slitlamp and in vivo confocal microscopy. SETTING:Departments of Ophthalmology, University of Lübeck, Lübeck, and University of Rostock, Rostock, Germany. DESIGN:Experimental study. METHODS:Corneas of rabbits were treated with different crosslinking (CXL) protocols. Two weeks after treatment, the corneas were evaluated in vivo by confocal microscopy. Eyes were enucleated and TPM was performed at 710 nm and 826 nm excitation wavelengths to detect tissue autofluorescence, second harmonic generation, and fluorescence lifetime measurements (FLIM). Eyes were then fixed and analyzed by histology. RESULTS:Crosslinking following the standard protocol generated a strong autofluorescence signal in the stroma that was detected by TPM. This signal was weakly present in the control specimens, and a sharp transition zone between the peripheral zone and the CXL zone was seen. On FLIM, an increase in corneal crosslinks was measured when the standard protocol was used. CONCLUSIONS:Two-photon microscopy, a noninvasive method, was able to detect the effects of therapeutic CXL and measure the grade of CXL. In addition to postoperative treatment control, the technique has possibilities for use in online dosimetry during 2-photon triggered CXL.
journal_name
J Cataract Refract Surgjournal_title
Journal of cataract and refractive surgeryauthors
Steven P,Hovakimyan M,Guthoff RF,Hüttmann G,Stachs Odoi
10.1016/j.jcrs.2010.06.068subject
Has Abstractpub_date
2010-12-01 00:00:00pages
2150-9issue
12eissn
0886-3350issn
1873-4502pii
S0886-3350(10)01386-6journal_volume
36pub_type
杂志文章abstract：PURPOSE:To study prospectively the success rate and complications of deep sclerectomy with collagen implant (DSCI), a nonpenetrating filtration procedure. SETTING:Glaucoma Unit, Department of Ophthalmology, Hôpital Ophtalmique Jules Gonin, University of Lausanne, Lausanne, Switzerland. METHODS:This nonrandomized pros...
journal_title：Journal of cataract and refractive surgery
pub_type： 临床试验,杂志文章
doi：10.1016/s0886-3350(01)01032-x
更新日期：2001-11-01 00:00:00
abstract：PURPOSE:To compare the inflammatory cell response within the corneal flap interface created by a mechanical microkeratome and a femtosecond laser. SETTING:Department of Ophthalmology, Ludwig-Maximilians-University, Munich, Germany. DESIGN:Experimental in vitro study. METHODS:Corneoscleral buttons of 12 enucleated hu...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2013.01.047
更新日期：2013-07-01 00:00:00
abstract：PURPOSE:To compare the accuracy of intraocular lens power prediction for eyes with average keratometry (K) readings greater than 46.00 diopters (D) and lower than 42.00 D. SETTING:Ein-Tal Eye Center, Tel-Aviv, Israel. DESIGN:Retrospective case series. METHODS:Eyes having cataract extraction surgery with steep and fl...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2017.06.036
更新日期：2017-09-01 00:00:00
abstract：PURPOSE:To determine whether residual myopia after primary laser-assisted subepithelial keratectomy (LASEK) can be corrected by repeated LASEK and whether this procedure is safe and effective. SETTING:University Eye Clinic, University of Regensburg, Regensburg, Germany. METHODS:Laser-assisted subepithelial keratectom...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(03)00253-0
更新日期：2003-07-01 00:00:00
abstract：:Anterior segment optical coherence tomography is a new method to explore the anterior chamber. The target can be focused and defocused with positive or negative lenses to reproduce the conditions of natural accommodation. We studied accommodation in an albino patient because the absence of pigment allows the infrared ...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2003.12.043
更新日期：2004-03-01 00:00:00
abstract：PURPOSE:To investigate the effect of surface modification of poly(methyl methacrylate) (PMMA) by poly(ethylene glycol) (PEG) grafting on cell adhesion. SETTING:Department of Ophthalmology, Seoul National University Hospital, Seoul, Korea. METHODS:The PMMA surface was oxidized with ozone, and PEG acrylate was then gra...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(00)00701-x
更新日期：2001-05-01 00:00:00
abstract：PURPOSE:To compare the long-term results of combined extracapsular cataract extraction (ECCE), intraocular lens (IOL) implantation, and trabeculectomy with those of trabeculectomy alone in low-risk patients. SETTING:An ophthalmic department in a British general district hospital. METHODS:We did a retrospective analys...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(96)80249-5
更新日期：1996-04-01 00:00:00
abstract：PURPOSE:To compare the 6-month results of accelerated and standard collagen crosslinking (CXL) treatment of progressive keratoconus. SETTING:Noor Eye Hospital, Tehran, Iran. DESIGN:Prospective randomized clinical trial. METHODS:Two groups of eyes (intervention and control) received corneal collagen crosslinking (CXL...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.jcrs.2014.07.030
更新日期：2015-03-01 00:00:00
abstract：PURPOSE:To evaluate the results and complications of hyperopic laser in situ keratomileusis (LASIK) with a low-energy femtosecond laser and non-custom tissue-saving ablation profile. SETTING:Private practice, Siena, Italy. DESIGN:Case series. METHODS:Hyperopic eyes with a spherical equivalent (SE) between +1.0 and +...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2013.11.031
更新日期：2014-07-01 00:00:00
abstract：:We developed a formula to measure the posterior curvature of the cornea. In our study, real corneal power in 51 eyes was calculated by using anterior and posterior curvatures and corneal thickness. The corneal refractive index was found to be a variable value between 1.3243 and 1.3339 (mean 1.3304 +/- 0.0003). ...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(13)80151-4
更新日期：1994-03-01 00:00:00
abstract：:This paper presents current clinical and experimental uses of the carbon dioxide laser and the results of our human and animal tissue fusion investigations. Trials were conducted with human scleral and corneal eye bank tissue and in albino rabbits. With power settings of 100 to 200 mW, optimal laser effect consisting ...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(87)80073-1
更新日期：1987-05-01 00:00:00
abstract：PURPOSE:To propose an alternative method to characterize postocclusion surge using a collapsible artificial anterior chamber to replace the currently used rigid anterior chamber model. SETTING:Fundación Oftamológica Los Andes, Santiago, Chile. METHODS:The distal end of a phacoemulsification handpiece was placed insid...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2005.03.061
更新日期：2005-10-01 00:00:00
abstract：PURPOSE:To assess the effectiveness of using anterior chamber irrigation of unpreserved lidocaine 1% as anesthesia during cataract surgery. SETTING:Private group ophthalmology practice. METHODS:This study prospectively evaluated 1000 of 1012 consecutive eyes having temporal corneal incision cataract surgery to determ...
journal_title：Journal of cataract and refractive surgery
pub_type： 临床试验,杂志文章
doi：10.1016/s0886-3350(97)80212-x
更新日期：1997-05-01 00:00:00
abstract：PURPOSE:To assess the safety and effectiveness of an immunotoxin, MDX-RA, designed to inhibit posterior capsule opacification (PCO). SETTING:Eleven private practices in the United States. METHODS:This study comprised 63 eyes of 63 patients having extracapsular cataract extraction by phacoemulsification; these patient...
journal_title：Journal of cataract and refractive surgery
pub_type： 临床试验,杂志文章,多中心研究,随机对照试验
doi：10.1016/s0886-3350(98)80352-0
更新日期：1998-12-01 00:00:00
abstract：:A 38-year-old woman had posterior chamber phakic intraocular lens (IOL) implantation as a secondary refractive procedure to correct residual refraction (20/50 with -16.50 -1.50 x 80) in May 1998, 3 years after intrastromal corneal ring segment surgery for high myopia (-30.00 diopters). Ultrasound biomicroscopy reveale...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(00)00410-7
更新日期：2000-05-01 00:00:00
abstract：:Positive pressure during cataract surgery can adversely affect the clinical outcome if the surgeon is unprepared. A variety of surgical maneuvers are described, including dry insertion of the phacoemulsification needle, capsule protection with the second instrument, dry cortical aspiration in an ophthalmic viscosurgic...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2006.06.011
更新日期：2006-09-01 00:00:00
abstract：PURPOSE:To assess the accuracy of surrogate decision making for elective cataract surgery. SETTING:Comprehensive Ophthalmology Department of Tertiary Care Hospital. METHODS:Decisions regarding elective cataract surgery of currently competent, elderly patients were compared with the predictions of patient-identified s...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2007.07.036
更新日期：2007-12-01 00:00:00
abstract：PURPOSE:To evaluate functional vision and quality-of-life outcomes after bilateral wavefront-guided laser in situ keratomileusis (LASIK). SETTING:Byers Eye Institute, Stanford University School of Medicine, Palo Alto, California, USA. DESIGN:Prospective case series. METHODS:Visual acuity, refractive error, and aberr...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2018.12.013
更新日期：2019-06-01 00:00:00
abstract：:Over a 12 month period, we used the anterior chamber maintainer (ACM) in cataract surgery in 258 patients; ages ranged from 15 to 95 years (mean 73 years). Surgery was performed using general or local anesthesia. The procedures were standard extracapsular cataract extraction (ECCE), mini-nuc ECCE, vectis extraction of...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(96)80214-8
更新日期：1996-03-01 00:00:00
abstract：PURPOSE:To compare posterior capsule opacification (PCO) after phacoemulsification and implantation of heparin-surface-modified (HSM) poly(methyl methacrylate) (PMMA) intraocular lenses (IOLs) in the capsular bag in patients with diabetes mellitus with that in a control group. SETTING:St. Erik's Eye Hospital, Stockhol...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(99)80132-1
更新日期：1999-02-01 00:00:00
abstract：:Safe, effective administration of local anesthesia prior to ocular surgery is a primary concern of ophthalmologists and variations in the technique and method of delivering anesthesia have been developed. Retrobulbar anesthesia, although considered safe and effective, can have serious complications. Using periocular a...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(13)80861-9
更新日期：1991-07-01 00:00:00
abstract：PURPOSE:To analyze the results of 1-way phacoemulsification and posterior chamber intraocular lens (IOL) implantation combined with trabeculectomy. SETTING:Department of Ophthalmology and Neurosurgery, University of Siena, Siena, Italy. METHODS:This retrospective study comprised 42 eyes of 36 patients with glaucoma a...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(99)00269-2
更新日期：1999-12-01 00:00:00
abstract：PURPOSE:To objectively evaluate the image quality obtained with toric intraocular lenses (IOLs) when misaligned from the intended axis. SETTING:University Eye Clinic and the Department of Industrial and Information Engineering, University of Trieste, Trieste, Italy. DESIGN:Experimental study. METHODS:An experimental...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2017.10.053
更新日期：2018-03-01 00:00:00
abstract：PURPOSE:To evaluate the change in higher-order aberrations (HOAs) after implantation of a foldable iris-claw phakic intraocular lens (pIOL) in myopic eyes. SETTING:Department of Ophthalmology, Johannes Gutenberg-University, Mainz, Germany. METHODS:This prospective nonrandomized comparative self-controlled trial inclu...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2005.12.036
更新日期：2006-02-01 00:00:00
abstract：PURPOSE:To compare carticaine 4% and lidocaine 2% for peribulbar anesthesia in cataract surgery. SETTING:Ain Shams and Cairo University Hospitals, Cairo, Egypt. METHODS:This prospective double-blind randomized study comprised 200 cataract patients assigned to 1 of 2 groups of 100 each based on type of anesthesia: car...
journal_title：Journal of cataract and refractive surgery
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0886-3350(01)01140-3
更新日期：2002-03-01 00:00:00
abstract：:Traumatic aniridia and aphakia occurred in the left eye of a 22-year-old man who had had Artisan (Ophtec) phakic intraocular lens (IOL) implantation. Aniridia was probably the result of the relatively large wound used to implant the nonfoldable IOL. Although the eye had severe traumatic damage, vision was recoverable ...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2007.03.037
更新日期：2007-07-01 00:00:00
abstract：PURPOSE:To compare the long-term longitudinal visual acuity outcomes after cataract surgery in eyes with age-related macular degeneration (AMD) at surgery and eyes without comorbidity. SETTING:University-based eye clinic. DESIGN:Longitudinal cohort study. METHODS:Patients having cataract surgery were evaluated over ...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2011.09.041
更新日期：2012-03-01 00:00:00
abstract：PURPOSE:To evaluate the safety of a new suprachoroidal device, the Cypass micro-stent, for the surgical treatment of open-angle glaucoma (OAG) implanted in conjunction with cataract surgery. SETTING:Multicenter clinical study. DESIGN:Prospective interventional case series. METHODS:This is an interim report of an ong...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章,多中心研究
doi：10.1016/j.jcrs.2012.10.040
更新日期：2013-03-01 00:00:00
abstract：PURPOSE:To evaluate the safety and complication rates of excimer laser photorefractive keratectomy (PRK). SETTING:Assutah Laser Center, Tel-Aviv, Israel. METHODS:This retrospective study evaluated the complication rate after PRK in 825 consecutive patients who had PRK for myopia and had a follow-up of at least 12 mon...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/s0886-3350(97)80311-2
更新日期：1997-10-01 00:00:00
abstract：:The ocular response analyzer (ORA) (Reichert, Inc.) was used in the case of a middle-aged man who developed unilateral corneal ectasia after bilateral laser in situ keratomileusis (LASIK). The preoperative refraction was similar in the 2 eyes. Post-LASIK ectasia was central in the left eye; topography was oblate in th...
journal_title：Journal of cataract and refractive surgery
pub_type： 杂志文章
doi：10.1016/j.jcrs.2007.11.018
更新日期：2008-03-01 00:00:00
