MDM2 promotes the proteasomal degradation of p73 through the interaction with Itch in HeLa cells.

abstract：:Using red cells as an experimental model, we previously showed that a limited degradation of certain membrane proteins by calpain (Ca2+-activated thiol protease) was a necessary prerequisite for cell fusion and that fusibility depended on the ratio of calpain to its endogenous inhibitor calpastatin. Here we show that ...

journal_title：Biochemical and biophysical research communications

authors： Barnoy S,Glasner T,Kosower NS

更新日期：1996-03-27 00:00:00

abstract：:Female Wistar rats were fed on a high fat diet for 18 weeks, during which their energy intake increased by 25% and body weight by 50% due to a doubling of adipose tissue tissue stores. Animals were then treated with increasing doses of a sheep polyclonal antiserum to rat adipocytes on days 1-4 and 7 after which they r...

journal_title：Biochemical and biophysical research communications

authors： Flint DJ

更新日期：1998-11-09 00:00:00

abstract：:CREB3 (Luman) is a family member of ER resident transcription factors, which are cleaved upon the induction of ER stress. Their N-terminal fragments shuttle into the nucleus where they regulate the transcription of target genes. Here, we found that human CREB3 is phosphorylated within its transcription activation doma...

journal_title：Biochemical and biophysical research communications

authors： Schmitt BM,Ampofo E,Stumpf H,Montenarh M,Götz C

更新日期：2020-03-12 00:00:00

abstract：:Collagen synthesis in human fibroblasts was increased by the presence of 0.1M excess NaCl in the culture medium. Type analysis of the collagen produced by the cells showed the increase in the ratio of type III collagen to the total collagen. Similar NaCl effects were observed in several cell lines derived from differe...

journal_title：Biochemical and biophysical research communications

authors： Hata R,Sunada H,Nagai Y

更新日期：1983-11-30 00:00:00

abstract：:The effect of glucocorticoid hormones on the protein responsible for both nucleotide pyrophosphatase (EC 3.6.1.9) and alkaline phosphodiesterase I (EC 3.1.4.1) activities was examined in murine MOPC 315 plasmacytoma cells. Incubation of these cells with dexamethasone resulted in parallel increases in pyrophosphatase a...

journal_title：Biochemical and biophysical research communications

authors： Rebbe NF,Hickman S

更新日期：1991-03-15 00:00:00

abstract：:We previously showed that nuclear factor 1-A (NF1-A) binds to the silencer elements in the glutathione transferase P (GST-P) gene, and the carboxy terminal region of NF1-A represses the transcription activity of human metallothionein IIA (hMTIIA) promoter. In this study, we identified a repression region which is divi...

journal_title：Biochemical and biophysical research communications

authors： Osada S,Ikeda T,Xu M,Nishihara T,Imagawa M

更新日期：1997-09-29 00:00:00

abstract：:We have previously shown that a ribozyme directed against human interleukin-6 (IL-6) mRNA is efficient in vivo, despite its poor activity in vitro on full-length IL-6 mRNA. We compared the effect of the nucleocapsid protein of HIV-1 (NCp7) on the ribozyme cleavage reaction of a long (1041 nt) and a short (19 nt) subst...

journal_title：Biochemical and biophysical research communications

authors： Mahieu M,Hendrix C,Ooms J,Herdewijn P,Content J

更新日期：1995-09-05 00:00:00

abstract：:We introduced a lentiviral vector containing the Sox11 gene into injured spinal cords of mice to evaluate the therapeutic potential of Sox11 in spinal cord injury. Sox11 markedly improved locomotor recovery after spinal cord injury and this recovery was accompanied by an up-regulation of Nestin/Doublecortin expression...

journal_title：Biochemical and biophysical research communications

authors： Guo Y,Liu S,Zhang X,Wang L,Zhang X,Hao A,Han A,Yang J

更新日期：2014-04-18 00:00:00

abstract：:Human Niemann-Pick type II fibroblasts, which encompass the panethnic type C (NPC) and Nova Scotia Acadian type D (NPD) variants, exhibit altered expression of caveolin-1 protein when examined by immunoblotting using an anti-caveolin-1 monoclonal antibody. Unexpectedly, caveolin-1 in heterozygous fibroblasts was signi...

journal_title：Biochemical and biophysical research communications

authors： Garver WS,Hsu SC,Erickson RP,Greer WL,Byers DM,Heidenreich RA

更新日期：1997-07-09 00:00:00

abstract：:A novel photoaffinity label, 8-N(3)-3'-biotinyl-ATP, has been synthesized. The introduction of an additional biotin residue is advantageous for easy detection of labeled proteins. This could be first tested by reaction with the F(1)-ATPase from the thermophilic bacterium PS3 (TF(1)). UV irradiation of TF(1) in the pre...

journal_title：Biochemical and biophysical research communications

authors： Schäfer HJ,Coskun U,Eger O,Godovac-Zimmermann J,Wieczorek H,Kagawa Y,Grüber G

更新日期：2001-09-07 00:00:00

abstract：:We cloned and sequenced a full-length cDNA of human placental N-acetylgalactosamine-6-sulfate sulfatase, the enzyme deficient in Morquio disease. The 2339-nucleotide sequence contained 1566 nucleotides which encoded a polypeptide of 522 amino acid residues. The deduced amino acid sequence was composed of a 26-amino ac...

journal_title：Biochemical and biophysical research communications

authors： Tomatsu S,Fukuda S,Masue M,Sukegawa K,Fukao T,Yamagishi A,Hori T,Iwata H,Ogawa T,Nakashima Y

更新日期：1991-12-16 00:00:00

abstract：:In the present study we evaluated the effect of interleukin 1-beta on adenylate cyclase activity in 235-1 pituitary cell line. The dose-response curve of interleukin 1 beta effect on adenylate cyclase activity showed a significant inhibition of basal enzyme activity at 1 pM concentration, while the inhibition of forsk...

journal_title：Biochemical and biophysical research communications

authors： Schettini G,Florio T,Meucci O,Scala G,Landolfi E,Grimaldi M

更新日期：1988-09-30 00:00:00

abstract：BACKGROUND AND OBJECTIVE:Senescence marker protein 30 (SMP30) is assumed to behave as an anti-aging factor. Recently, we have demonstrated that deficiency of SMP30 exacerbates angiotensin II-induced cardiac hypertrophy, dysfunction and remodeling, suggesting that SMP30 may have a protective role in the heart. Thus, thi...

journal_title：Biochemical and biophysical research communications

authors： Misaka T,Suzuki S,Miyata M,Kobayashi A,Ishigami A,Shishido T,Saitoh S,Kubota I,Takeishi Y

更新日期：2013-09-13 00:00:00

abstract：:The novel synthetic retinoid, CD437, shows potent anti-tumor activity in a range of different cancer cell lines and now serves as a prototype for development of new retinoid related molecules (RRMs). The purpose of this study was to examine the effect and cellular targets of CD437 in the human metastatic melanoma cell...

journal_title：Biochemical and biophysical research communications

authors： Magnussen GI,Ree Rosnes AK,Shahzidi S,Dong HP,Emilsen E,Engesæter B,Flørenes VA

更新日期：2012-04-13 00:00:00

abstract：:To elucidate whether PTH(7-84), a degradation product of PTH(1-84), which inhibits PTH(1-84)-induced bone resorption, also exerts an antagonistic effect on the kidney, we studied the effect of PTH(7-84) on PTH(1-34)-induced production of 1,25-(OH)2D3 in primary cultured murine renal tubules. Neonatal mouse renal tubul...

journal_title：Biochemical and biophysical research communications

authors： Nakajima K,Nohtomi K,Sato M,Takano K,Sato K

更新日期：2009-04-03 00:00:00

abstract：:Glycosylation mutants of chinese hamster ovary cells were used to analyse the role played by surface-exposed carbohydrates on the process of interaction of Trypanosoma cruzi with the host cell. Adhesion and invasion of the parasites were markedly reduced in cells which express very few sialic acid residues. Infection ...

journal_title：Biochemical and biophysical research communications

authors： Ciavaglia Mdo C,de Carvalho TU,de Souza W

更新日期：1993-06-15 00:00:00

abstract：:The diastereomeric GSH derivatives gamma-L-Glu-L-allo-thioThr-Gly (6) and gamma-L-Glu-L-thioThr-Gly (6a) have been synthesized as specific probes of the steric environment near the cysteinyl residue of enzyme bound glutathionyl substrates. Experiments with glyoxalase I indicate that while 6a-methylglyoxal thiohemiacet...

journal_title：Biochemical and biophysical research communications

authors： Xie XF,Creighton DJ

更新日期：1991-05-31 00:00:00

abstract：:We cloned a cDNA encoding a novel extracellular signal-regulated kinase (ERK) 2-binding protein, EBITEIN1, by yeast two-hybrid screening. Northern and Western blotting experiments showed that the transcript and protein were expressed in the testes. Furthermore, immunohistochemical experiments showed that EBITEIN1 exis...

journal_title：Biochemical and biophysical research communications

authors： Miura K,Imaki J

更新日期：2008-04-04 00:00:00

abstract：:The primary structure of human selenium-dependent phospholipid hydroperoxide glutathione peroxidase (GPX4) was determined by genomic cloning. The gene structure of GPX4 spans only 2.8 kb and consists of 7 exons. The coding sequence resides on all 7 exons, and the mitochondrial leader sequence is contained entirely wit...

journal_title：Biochemical and biophysical research communications

authors： Kelner MJ,Montoya MA

更新日期：1998-08-10 00:00:00

abstract：:Didemnin B, a cyclic N-methylated peptolide induces apoptosis in human HL-60 cells. When incubated with 1 microM didemnin B, unsynchronized HL-60 cultures undergo apoptosis to 100% within 140 minutes. Apoptosis has been assessed by the typical apoptotic morphology, the presence of double-stranded DNA fragments within ...

journal_title：Biochemical and biophysical research communications

authors： Grubb DR,Wolvetang EJ,Lawen A

更新日期：1995-10-24 00:00:00

abstract：:Homologous-recombination (HR)-dependent repair defective cells are hypersensitive to poly (ADP-ribose) polymerase (PARP) inhibitors. Combinations of defective HR pathway and PARP inhibitors have been an effective chemotherapeutic modality. We previously showed that knockdown of the WD40-repeat containing protein, Uaf1...

journal_title：Biochemical and biophysical research communications

authors： Choi YE,Park E

更新日期：2015-03-13 00:00:00

abstract：:An angiogenesis inhibitor named Beta-35 has been identified and purified from the conditioned medium of mouse pancreatic β cells tumor cells. Beta-35 has a molecular weight of 35 kDa and inhibits DNA synthesis of bovine capillary endothelial cells at a half-maximal concentration of approximately 5 nM. It shows anti-an...

journal_title：Biochemical and biophysical research communications

authors： Liang G,Butterfield C,Liang J,Birsner A,Folkman J,Shing Y

更新日期：2011-06-10 00:00:00

abstract：:We investigated the expression of myocilin in the optic nerve head of porcine eyes by Western blotting and immunohistochemical staining. Myocilin was localized in the nucleus, centrosome, glial filament, mitochondria, and some parts of the cell membranes of the astrocytes. Myocilin was also detected at the edge-feet p...

journal_title：Biochemical and biophysical research communications

authors： Noda S,Mashima Y,Obazawa M,Kubota R,Oguchi Y,Kudoh J,Minoshima S,Shimizu N

更新日期：2000-10-05 00:00:00

abstract：:Matrix metalloproteinases (MMPs) degrade extracellular matrix proteins, which may be responsible for enlargement of alveoli in chronic obstructive pulmonary disease (COPD) and remodeling of pulmonary tissue associated with chronic asthma. Here, we provide novel evidence that MMP-12 is expressed and secreted by normal ...

journal_title：Biochemical and biophysical research communications

authors： Lavigne MC,Thakker P,Gunn J,Wong A,Miyashiro JS,Wasserman AM,Wei SQ,Pelker JW,Kobayashi M,Eppihimer MJ

更新日期：2004-11-12 00:00:00

abstract：:The expression and secretion of Transforming Growth Factor beta1 (TGFbeta1) by cultured testes of day 20.5 rat fetuses were investigated. The testes were found to express two TGFbeta1 mRNA transcripts of 2.5 and 1.8 kb. By using mink lung epithelial cell bioassay based on the measurement of the inhibition of tritiated...

journal_title：Biochemical and biophysical research communications

authors： Gautier C,Levacher C,Saez JM,Habert R

更新日期：1997-07-09 00:00:00

abstract：:F(0)F(1)-ATPase within chromatophore was constructed as a biosensor (immuno-rotary biosensor) for the purpose of capturing single virus. Capture of virus was based on antibody-antigen reaction. The detection of virus based on proton flux change driven by ATP-synthesis of F(0)F(1)-ATPase, which was indicated by F1300, ...

journal_title：Biochemical and biophysical research communications

authors： Liu X,Zhang Y,Yue J,Jiang P,Zhang Z

更新日期：2006-04-21 00:00:00

abstract：:C3 is common to all pathways of complement activation augmenting ischemia/reperfusion (I/R)-induced myocardial injury and cardiac dysfunction. Complement inhibition with the complement regulatory protein, C1 inhibitor (C1INH), obviously exerts cardioprotective effects. Here, we examine whether C1INH regulates C3 activ...

journal_title：Biochemical and biophysical research communications

authors： Fu J,Lin G,Zeng B,Wu Z,Wu Y,Chu H,Qin G,Liang G,Li J,Gan X,Yu X,Li C,Liu D

更新日期：2006-11-10 00:00:00

abstract：:The small GTPase Rheb binds and activates mTORC1, which plays a pivotal role in diverse cellular physiologies. To increase our understanding of how Rheb regulates mTORC1 signaling, we set out to identify Rheb binding proteins using shotgun proteomics approaches. In this study, we characterized HSP70, one of the identi...

journal_title：Biochemical and biophysical research communications

authors： Ryu HH,Ha SH

更新日期：2020-12-17 00:00:00

abstract：:Here we describe the molecular cloning of human cyclin B3, its localization, and its structure. It is localized in the subcentromeric region of the X chromosome, still not completely sequenced by the Human Genome Project. This cyclin B3 is unusually large for a mitotic cyclin. Its mRNAs were found in all tissues and w...

journal_title：Biochemical and biophysical research communications

authors： Lozano JC,Perret E,Schatt P,Arnould C,Peaucellier G,Picard A

更新日期：2002-02-22 00:00:00

abstract：:We have examined the restriction digest patterns of CCGG sequences in Kiras, Ha-ras, and c-myc oncogenes in rat tracheal epithelial cells transformed in vitro by 7,12-dimethylbenz(a)anthracene, benzo(a)pyrene/12-O-tetradecanoylphorbol-13-acetate (TPA), or TPA alone. Oncogenes c-myc and Ha-ras in transformed cell lines...

journal_title：Biochemical and biophysical research communications

authors： Mass MJ,Schorschinsky NS,Lasley JA,Beeman DK,Austin SJ

更新日期：1989-10-31 00:00:00