Abstract:
:Lung cancer is still difficult to treat by current chemotherapeutic procedures. We recently found that MVL, an anti-HIV lectin from blue-green algae Microcystis viridis, also has antitumor activity. The objective of this study was to investigate apoptosis-inducing activity of recombinant MVL (R-MVL) and proteomic changes in A549 cells, and to identify the molecular pathways responsible for the anti-cancer action of R-MVL. We found that R-MVL induces A549 cells apoptosis in a dose-dependent manner by using MTT assay, fluorescent microscope (FM) and flow cytometry (FCM), and the IC50 was calculated to be 24.12 μg/ml. Subsequently, 7 altered proteins in R-MVL-treated A549 cells were identified, including upregulated aldehyde dehydrogenase 1 and β-actin, and five downregulated proteins: heat shock protein 90, heat shock 60, plastin 3, tropomyosin 3, and β-tubulin. Further bioinformatics analysis predicted the potential pathways for R-MVL to induce apoptosis of A549 cells. In conclusion, this is the first report to investigate anti-cancer activity of R-MVL and its mechanism of action by proteomics analysis. Our observations provide potential therapeutic targets for lung cancer inhibitor intervention and implicated the development of novel anti-cancer therapeutic strategies.
journal_name
Amino Acidsjournal_title
Amino acidsauthors
Li Y,Zhang B,Wang X,Yan H,Chen G,Zhang Xdoi
10.1007/s00726-010-0791-0subject
Has Abstractpub_date
2011-10-01 00:00:00pages
923-32issue
4eissn
0939-4451issn
1438-2199journal_volume
41pub_type
杂志文章相关文献
AMINO ACIDS文献大全abstract::Arginine adenosine-5'-diphosphoribosylation (ADP-ribosylation) is an enzyme-catalyzed, potentially reversible posttranslational modification, in which the ADP-ribose moiety is transferred from NAD(+) to the guanidino moiety of arginine. At 540 Da, ADP-ribose has the size of approximately five amino acid residues. In c...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-010-0676-2
更新日期:2011-07-01 00:00:00
abstract::High-protein diets have been shown to alleviate detrimental effects of high-fat diets and this effect can be partially mimicked by dietary L-leucine supplementation. Here, we aimed to elucidate the early mechanisms and the specificity of leucine effects. We performed a 1-week trial with male C57BL/6 mice fed ad libitu...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1363-2
更新日期:2013-02-01 00:00:00
abstract::The influence of nitric oxide synthase (NOS) activity on the KCl-evoked amino acid concentrations was investigated by in vivo microdialysis in the striatum in a rat model of excitotoxic lesion. Basal microdialysate levels of amino acids decreased during the quinolinic acid-induced neurodegeneration process, except for...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s007260070021
更新日期:2000-01-01 00:00:00
abstract::Taurine depletion leads to impaired mitochondrial function, as characterized by reduced ATP production and elevated superoxide generation. These defects can fundamentally alter cardiomyocyte function and if left unchanged can result in cell death. To protect against these stresses, cardiomyocytes possess quality contr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-015-2053-7
更新日期:2015-12-01 00:00:00
abstract::Early evaluation of cancer response to a therapeutic regimen can help increase the effectiveness of treatment schemes and, by enabling early termination of ineffective treatments, minimize toxicity, and reduce expenses. Biomarkers that provide early indication of tumor therapy response are urgently needed. Solid tumor...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0548-9
更新日期:2011-11-01 00:00:00
abstract::M33 is a branched peptide currently under preclinical characterization for the development of a new antibacterial drug against gram-negative bacteria. Here, we report its pegylation at the C-terminus of the three-lysine-branching core and the resulting increase in stability to Pseudomonas aeruginosa elastase. This pro...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1686-2
更新日期:2014-05-01 00:00:00
abstract::Based on ubiquitous presence of large ionic motifs and clusters in proteins involved in gene transcription and protein synthesis, we analyzed the distribution of ionizable sidechains in a broad selection of proteins with regulatory, metabolic, structural and adhesive functions, in agonist, antagonist, toxin and antimi...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-012-1418-4
更新日期:2012-12-01 00:00:00
abstract::This work deals with the Dakin-West synthesis, starting from the nucleoamino acid 1-thyminyl acetic acid, as well the NMR, ESI MS, and X-ray characterization of a heteroaromatic compound denominated by us T(2)CO, comprising two thymine moieties anchored to a 2-propanonic unit, the spectroscopic properties of which wer...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-012-1237-7
更新日期:2012-10-01 00:00:00
abstract::Skin collagen metabolism abnormalities induced by ultraviolet (UV) radiation are the major causes of skin photoaging. It has been shown that the one-time exposure of UV irradiation decreases procollagen mRNA expression in dermis and that chronic UV irradiation decreases collagen amounts and induces wrinkle formation. ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-011-1059-z
更新日期:2012-06-01 00:00:00
abstract::We describe six children with tetrahydrobiopterin (BH(4)) responsive phenylalanine hydroxylase (PAH) deficiency. All patients carry two mutant alleles in the PAH gene. Cofactor deficiency was excluded. The effect of BH(4) administration was studied by correlating different oral BH(4) doses with plasma phenylalanine le...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-002-0354-0
更新日期:2003-07-01 00:00:00
abstract::A subline growing in medium without arginine and ornithine was established from a rat Reuber hepatoma cell line (R-Y121B·cho). The subline designated R-Y117B·cho was able to grow in glutamine, arginine and ornithine-free, glutamate-supplemented medium. Arginine synthesis from glutamate requires four urea cycle enzymes...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00807328
更新日期:1996-09-01 00:00:00
abstract::The regulation of the expression of indoleamine 2,3-dioxygenase (IDO) was studied in cloned murine macrophages (MT2) and microglial (N11) cells. Both cell lines express IDO and inducible nitric oxide synthase activity after interferon-gamma (IFN-gamma) stimulation. The regulation of IDO expression appears to differ in...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01345271
更新日期:1998-01-01 00:00:00
abstract::The purpose of the present study was to investigate the relationship between plasma carnitine concentration and body composition variation in relation to muscular and fat masses since there is no experimentally proved correlation between plasma carnitine and body masses. We used bioelectric impedance analysis (BIA), t...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01318855
更新日期:1998-01-01 00:00:00
abstract::We report the solid phase synthesis and some pharmacological properties of 24 oxytocin (OT) analogues. Basic modifications at position 9 (introduction of L- or D-beta-(2-thienyl)-alanine [L- or D-Thi], or L- or D-3-Pyridylalanine [L- or D-3-Pal]) were combined with D-tyrosine(OEthyl) [D-Tyr(Et)] or D-1-naphthylalanine...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0372-2
更新日期:2010-05-01 00:00:00
abstract::Based on previous research with bovine peadipocytes, we hypothesized that infusion of arginine into the abomasum of Angus steers stimulates stearoyl-CoA desaturase (SCD) gene expression in bovine subcutaneous (s.c.) adipose tissue, and that this would be attenuated by conjugated linoleic acid (CLA). Growing Angus stee...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-013-1622-x
更新日期:2014-02-01 00:00:00
abstract::The effect of dietary amino acid taurine on the liver function of chronic hepatitis patients was investigated. The 24 chronic hepatitis patients with 2-5 times over normal activities of alanine aminotransferase (ALT) or aspartate aminotransferase (AST) were selected and equally divided into taurine treatment and contr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-007-0565-5
更新日期:2008-08-01 00:00:00
abstract::The expression of wild type CFTR facilitates the efflux of neutral amino acids (Rotoli et al., Biochem. Biophys. Res. Commun. 204: 653-658, 1994); as a result, after an extensive depletion of intracellular amino acid pool obtained through an incubation in saline solution, the intracellular leucine levels were lower in...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF00813863
更新日期:1996-06-01 00:00:00
abstract::Taurine is known to increase ATP-dependent calcium ion (Ca2+) uptake in retinal membrane preparations and in isolated rod outer segments (ROS) under low calcium conditions (10 microM) (Pasantes-Morales and Ordóñez, 1982; Lombardini, 1991). In this report, ATP-dependent Ca2+ uptake in retinal membrane preparations was ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01345283
更新日期:1998-01-01 00:00:00
abstract::In living cells, the N-terminal tails of core histones, the proteinaceous component of nucleosomes, are subjected to a range of covalent post-translational modifications (PTMs), which have specific roles in modulating chromatin structure and function. A growing body of evidence suggests that deregulation of histone mo...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-010-0668-2
更新日期:2011-07-01 00:00:00
abstract::The G protein-coupled receptor kinase (GRK) family consists of seven cytosolic serine/threonine (Ser/Thr) protein kinases, and among them, GRK2 is involved in the regulation of an enormous range of both G protein-coupled receptors (GPCRs) and non-GPCR substrates that participate in or regulate many critical cellular p...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-020-02864-x
更新日期:2020-07-01 00:00:00
abstract::A variety of alpha-amino acids are enantioresolved for the first time on naphthylethylcarbamate-beta-cyclodextrin bonded phases (i.e., SN- and RN-beta-CD) using the acetonitrile-based mobile phase after their pre-column derivatization with phenyl isothiocyanate in alkaline medium. The resolution is better obtained on ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-003-0038-4
更新日期:2004-06-01 00:00:00
abstract::This study examines the hypothesis that glutamate tonically suppresses the activity of the enzyme aromatic L-amino acid decarboxylase (AADC), and hence the biosynthesis of dopamine, to explain how antagonists of glutamate receptors might potentiale the motor actions of L-DOPA in animal models of Parkinson's disease. A...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01345241
更新日期:1998-01-01 00:00:00
abstract::Two experiments were conducted to evaluate the effects of dietary supplementation with Astragalus polysaccharide (APS) on growth performance, apparent ileal digestibilities (AID) of amino acids (AA), and their serum concentrations in early weaned piglets. In Exp. 1, 60 pigs were weaned at 21 days of age (BW 7.35 +/- 0...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0142-6
更新日期:2009-07-01 00:00:00
abstract::Recovery of amino acid (AA) metabolism and the associated clinical implications in chronic hepatitis C (CHC) patients with sustained virological response (SVR) following anti-hepatitis C virus (HCV) therapy remains elusive. A prospective cohort study was conducted on 222 CHC patients with SVR. Eighty-two age-matched m...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-016-2360-7
更新日期:2017-02-01 00:00:00
abstract::To evaluate the response of soybean to salt stress, the related changes in protein expression were investigated using the proteomic approach. Soybean plants were exposed to 0, 50, 100, and 200 mM NaCl. Especially at 200 mM, the length and fresh weight of the hypocotyl and root reduced under salt stress, while the prol...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-008-0036-7
更新日期:2009-01-01 00:00:00
abstract::Endurance exercise training as well as leucine supplementation modulates glucose homeostasis and protein turnover in mammals. Here, we analyze whether leucine supplementation alters the effects of endurance exercise on these parameters in healthy mice. Mice were distributed into sedentary (C) and exercise (T) groups. ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-014-1903-z
更新日期:2015-04-01 00:00:00
abstract::Knowing the key features of the structure and the biochemistry of proteins is crucial to improving enzymes of industrial interest like β-fructofuranosidase. Gene sacA from Bacillus licheniformis ATCC 14580 codifies a sucrose-6-phosphate hydrolase, a β-fructofuranosidase (E.C. 3.1.2.26, protein BlsacA), which has no cr...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-018-02690-2
更新日期:2019-04-01 00:00:00
abstract::Immobilization of papain on Sepharose 6B in the presence of different concentrations of cysteine affected the enzyme activity depending on cysteine concentration. The maximum specific activity was observed when papain was immobilized with 200 mM cysteine. The immobilization process brought significant enhancement of s...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/s00726-009-0302-3
更新日期:2010-03-01 00:00:00
abstract::Dopa and related catecholamines and their degradation products have been demonstrated to have neurotoxic potential in a number of cellular and in vivo experiments. Several mechanisms have been hypothesized to be involved including generation of prooxidant products that subsequently oxidize membrane lipids and exposed ...
journal_title:Amino acids
pub_type: 杂志文章
doi:10.1007/BF01345261
更新日期:1998-01-01 00:00:00
abstract::Obesity, type 2 diabetes (T2DM) and cardiovascular disease (CVD) are the most common preventable causes of morbidity and mortality worldwide. They represent major public health threat to our society. Increasing prevalence of obesity and T2DM contributes to escalating morbidity and mortality from CVD and stroke. Carnos...
journal_title:Amino acids
pub_type: 杂志文章,评审
doi:10.1007/s00726-016-2208-1
更新日期:2016-05-01 00:00:00