当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY期刊下所有文献 > Combining two potential causes of metalloproteinase secretion causes abdominal aortic aneurysms in rats: a new experimental model.

Combining two potential causes of metalloproteinase secretion causes abdominal aortic aneurysms in rats: a new experimental model.

Abstract：

:Progress in understanding the pathophysiology of abdominal aortic aneurysms (AAA) is dependent in part on the development and application of effective animal models that recapitulate key aspects of the disease. The objective was to produce an experimental model of AAA in rats by combining two potential causes of metalloproteinase (MMP) secretion: inflammation and turbulent blood flow. Male Wistar rats were randomly divided in four groups: Injury, Stenosis, Aneurysm and Control (40/group). The Injury group received a traumatic injury to the external aortic wall. The Stenosis group received an extrinsic stenosis at a corresponding location. The Aneurysm group received both the injury and stenosis simultaneously, and the Control group received a sham operation. Animals were euthanized at days 1, 3, 7 and 15. Aorta and/or aneurysms were collected and the fragments were fixed for morphologic, immunohistochemistry and morphometric analyses or frozen for MMP assays. AAAs had developed by day 3 in 60-70% of the animals, reaching an aortic dilatation ratio of more than 300%, exhibiting intense wall remodelling initiated at the adventitia and characterized by an obvious inflammatory infiltrate, mesenchymal proliferation, neoangiogenesis, elastin degradation and collagen deposition. Immunohistochemistry and zymography studies displayed significantly increased expressions of MMP-2 and MMP-9 in aneurysm walls compared to other groups. The haemo-dynamic alterations caused by the stenosis may have provided additional contribution to the MMPs liberation. This new model illustrated that AAA can be multifactorial and confirmed the key roles of MMP-2 and MMP-9 in this dynamic remodelling process.

journal_name

Int J Exp Pathol

journal_title

International journal of experimental pathology

authors

Mata KM,Prudente PS,Rocha FS,Prado CM,Floriano EM,Elias J Jr,Rizzi E,Gerlach RF,Rossi MA,Ramos SG

doi

10.1111/j.1365-2613.2010.00746.x

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

26-39

issue

eissn

0959-9673

issn

1365-2613

journal_volume

pub_type

杂志文章

在线工具

Combining two potential causes of metalloproteinase secretion causes abdominal aortic aneurysms in rats: a new experimental model.