Abstract:
:The recent discovery of microRNAs (miRNAs) has revealed a new layer of gene expression regulation, affecting the immune system. Here, we identify their roles in regulating human plasmacytoid dendritic cell (PDC) activation. miRNA profiling showed the significantly differential expression of 19 miRNAs in PDCs after Toll-like receptor 7 (TLR7) stimulation, among which miR-155* and miR-155 were the most highly induced. Although they were processed from a single precursor and were both induced by TLR7 through the c-Jun N-terminal kinase pathway, miR-155* and miR-155 had opposite effects on the regulation of type I interferon production by PDC. Further study indicated that miR-155* augmented interferon-α/β expression by suppressing IRAKM, whereas miR-155 inhibited their expression by targeting TAB2. Kinetic analysis of miR-155* and miR-155 induction revealed that miR-155* was mainly induced in the early stage of stimulation, and that miR-155 was mainly induced in the later stage, suggesting their cooperative involvement in PDC activation. Finally, we demonstrated that miR-155* and miR-155 were inversely regulated by autocrine/paracrine type I interferon and TLR7-activated KHSRP at the posttranscriptional level, which led to their different dynamic induction by TLR7. Thus, our study identified and validated novel miRNA-protein networks involved in regulating PDC activation.
journal_name
Bloodjournal_title
Bloodauthors
Zhou H,Huang X,Cui H,Luo X,Tang Y,Chen S,Wu L,Shen Ndoi
10.1182/blood-2010-04-280156subject
Has Abstractpub_date
2010-12-23 00:00:00pages
5885-94issue
26eissn
0006-4971issn
1528-0020pii
blood-2010-04-280156journal_volume
116pub_type
杂志文章相关文献
BLOOD文献大全abstract::The therapeutic potential of hematopoietic stem cell (HSC) gene therapy can be fully exploited only by reaching efficient gene transfer into HSCs without compromising their biologic properties. Although HSCs can be transduced by HIV-derived lentiviral vectors (LVs) in short ex vivo culture, they display low permissivi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-10-4047
更新日期:2006-06-01 00:00:00
abstract::In this issue of Blood, Rieger et al show that malaria parasite infiltration in the human placenta requires a specific geometry and affinity of host receptors to facilitate strong adhesion. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2014-11-610634
更新日期:2015-01-08 00:00:00
abstract::Perforin (Prf1) and granzyme B (GzmB) are essential effector molecules for natural killer (NK)-cell cytotoxicity, but how Prf1 and GzmB expression is regulated during arming of NK cells is poorly defined. We show that human microRNA (miR)-27a* is a negative regulator of NK-cell cytotoxicity by silencing Prf1 and GzmB ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-04-347526
更新日期:2011-11-17 00:00:00
abstract::A 2-yr-old black girl presented with a thalassemic clinical picture and was found to have nearly 100% fetal hemoglobin in her red cells. Pedigree analysis indicated that she was a heterozygote for the hereditary persistence of fetal hemoglobin gene and for a beta O-thalassemia gene. A brother, who also had nearly 100%...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1981-06-01 00:00:00
abstract::Serine proteinase inhibitors (serpins) play a vital regulatory role in a wide range of biological processes, and serpins from viruses have been implicated in pathogen evasion of the host defence system. For the first time, we report a functional serpin gene from nematodes that may function in this manner. This gene, n...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-08-15 00:00:00
abstract::Chronic lymphocytic leukemia (CLL) is an incurable adult disease of unknown etiology. Understanding the biology of CLL cells, particularly cell maturation and growth in vivo, has been impeded by lack of a reproducible adoptive transfer model. We report a simple, reproducible system in which primary CLL cells prolifera...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-12-324210
更新日期:2011-05-19 00:00:00
abstract::Dendritic cells (DCs) represent a family of ontogenically distinct leukocytes involved in immune response regulation. The ability of DCs to stimulate T-cell immunity has led to their use as vectors for immunotherapy vaccines. However, it is unclear whether and to what degree in vitro-generated DCs are representative o...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::Whether resveratrol, a component of red grapes, berries, and peanuts, could suppress the proliferation of multiple myeloma (MM) cells by interfering with NF-kappaB and STAT3 pathways, was investigated. Resveratrol inhibited the proliferation of human multiple myeloma cell lines regardless of whether they were sensitiv...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-02-003988
更新日期:2007-03-15 00:00:00
abstract::MicroRNAs (miRNAs) regulate cell physiology by altering protein expression, but the biology of platelet miRNAs is largely unexplored. We tested whether platelet miRNA levels were associated with platelet reactivity by genome-wide profiling using platelet RNA from 19 healthy subjects. We found that human platelets expr...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-09-299719
更新日期:2011-05-12 00:00:00
abstract::IL-12 exerts several regulatory effects on natural killer (NK) cells by activating IL-12 signaling. IL-12 signaling is tightly auto-regulated to control its onset and termination, with prolonged IL-12 treatment resulting in IL-12 hyporesponsiveness. However, the mechanisms underlying IL-12 auto-regulation are still un...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-05-356162
更新日期:2011-12-22 00:00:00
abstract::The development of neutralizing antibodies (inhibitors) against coagulation factor VIII (FVIII) is the most problematic and costly complication of FVIII replacement therapy that affects up to 30% of previously untreated patients with severe hemophilia A. The development of inhibitors is a multifactorial complication i...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-12-685735
更新日期:2016-06-09 00:00:00
abstract::Combined deficiency of ankyrin and spectrin represents the most common biochemical abnormality in hereditary spherocytosis (HS). To examine whether a decrease in ankyrin mRNA represents a frequent cause of this type of HS, we took advantage of the reported (AC)n microsatellite polymorphism in the 3' untranslated regio...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1995-06-01 00:00:00
abstract::The tyrosine kinase receptor fetal liver kinase 1 (Flk-1) plays a crucial role in vasculogenesis and angiogenesis, but its target genes remain elusive. Comparing Flk-1(+/+) with Flk-1(-/-) embryonic stem (ES) cells, we identified transcripts regulated by the vascular endothelial growth factor A (VEGF-A)/Flk-1 pathway ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-11-3827
更新日期:2004-06-15 00:00:00
abstract::Microparticles (MPs) are submicron extracellular vesicles exposing phosphatidylserine (PS), detected at high concentration in the circulation of sickle cell anemia (SS) patients. Several groups studied the biological effects of MPs generated ex vivo. Here, we analyzed for the first time the impact of circulating MPs o...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2020004853
更新日期:2020-07-09 00:00:00
abstract::The urokinase receptor (uPAR) plays an important role in regulation of fibronolysis, cell migration, and adhesion. In this study, we examined whether uPAR plays a role in modulating efferocytosis of neutrophils. Macrophages from uPAR(-/-) mice demonstrated enhanced ability to engulf viable wild-type (WT) neutrophils i...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-12-193524
更新日期:2009-07-23 00:00:00
abstract::Intravascular hemolysis describes the relocalization of heme and hemoglobin (Hb) from erythrocytes to plasma. We investigated the concept that erythrocyte membrane microparticles (MPs) concentrate cell-free heme in human hemolytic diseases, and that heme-laden MPs have a physiopathological impact. Up to one-third of c...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-07-589283
更新日期:2015-06-11 00:00:00
abstract::The present studies investigated the effects of the recently cloned flt3 ligand (FL) on the in vitro growth and differentiation of primitive and committed subsets of human CD34+ bone marrow (BM) progenitor cells. FL alone was a weak growth stimulator of CD34+ BM cells, but synergistically and directly enhanced colony ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-02-15 00:00:00
abstract::Evidence is presented showing that a protein tyrosine phosphatase different from CD45 is present on the membrane of human hematopoietic cells. The molecule recognized by the monoclonal antibody 143-41, which has been classified as CD148 in the VI International Workshop on Leukocyte Differentiation Antigens, was immuno...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-04-15 00:00:00
abstract::Bleeding problems are associated with defects in platelet alpha-granules, yet little is known about how these granules are formed and released. Mutations affecting VPS33B, a novel Sec1/Munc18 protein, have recently been linked to arthrogryposis, renal dysfunction, and cholestasis (ARC) syndrome. We have characterized ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2005-04-1356
更新日期:2005-12-15 00:00:00
abstract::Recent studies performed in mice knocked out for the tumor necrosis factor (TNF ), the lymphotoxin-alpha, or the type I TNF receptor (R), genes have shown that these animals display gross defects in germinal center (GC) formation, suggesting that members of the TNF and TNFR superfamilies are involved in the control of...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-12-01 00:00:00
abstract::We have previously described the development of oncoretrovirus vectors for human gamma-globin using a truncated beta-globin promoter, modified gamma-globin cassette, and alpha-globin enhancer. However, one of these vectors is genetically unstable, and both vectors exhibit variable expression patterns in cultured cells...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-01-0219
更新日期:2002-09-15 00:00:00
abstract::Choosing Wisely® is a medical stewardship and quality improvement initiative led by the American Board of Internal Medicine Foundation in collaboration with leading medical societies in the United States. The ASH is an active participant in the Choosing Wisely® project. Using an iterative process and an evidence-based...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-07-518423
更新日期:2013-12-05 00:00:00
abstract::Immunoaffinity chromatography using conformation-specific antibodies yields pure factor IX from human plasma in a single rapid, facile purification step. We evaluated this technique to determine whether factor IX can be separated from human T cell leukemia virus-I (HTLV-I) and human immunodeficiency virus (HIV) in pla...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1987-11-01 00:00:00
abstract::Pain is the leading cause of emergency department visits, hospitalizations, and daily suffering in individuals with sickle cell disease (SCD). The pathologic mechanisms leading to the perception of pain during acute RBC sickling episodes and development of chronic pain remain poorly understood and ineffectively treate...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-12-327429
更新日期:2011-09-22 00:00:00
abstract::Deletions at the 3' end of the human beta-globin locus are associated with the hereditary persistence of fetal hemoglobin (HPFH) in adults, potentially through the juxtaposition of enhancer elements in the vicinity of the fetal gamma-globin genes. We have tested how sequences at the HPFH-2, HPFH-3, and HPFH-6 breakpoi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-05-1681
更新日期:2003-11-01 00:00:00
abstract::GVHD is still one of the major complications after allogeneic stem cell transplantation. Whereas murine data have clearly shown the beneficial effects of regulatory T cells (Tregs) on the prevention of GVHD, data from the human system are rare. Here, we present a comparative dynamic analysis of CD4(+)CD25(hi)CD127(lo/...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-05-352708
更新日期:2011-09-29 00:00:00
abstract::It has been proposed that CD4 T-cell responses to Staphylococcus aureus (SA) can inadvertently enhance neoplastic progression in models of skin cancer and cutaneous T-cell lymphoma (CTCL). In this prospective study, we explored the effect of transient antibiotic treatment on tumor cells and disease activity in 8 patie...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2018888107
更新日期:2019-09-26 00:00:00
abstract::Nonresponse to remission-induction chemotherapy, which remains a major problem in acute myeloblastic leukemia (AML), has been linked to cellular resistance to apoptosis. Because the apoptosis induced by chemotherapeutic drugs is mediated by loss of mitochondrial transmembrane potential (MTP), it was postulated that se...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.2.405
更新日期:2001-07-15 00:00:00
abstract::Interleukin-1 (IL-1) has been shown to ameliorate the hematopoietic toxicities of antitumor chemotherapeutic agents in both mice and humans. However, IL-1 toxicity in humans is considerable and is similar to the systemic inflammatory toxicities induced by IL-3, IL-6, and other cytokines with pleiotropic biologic activ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1994-09-01 00:00:00
abstract::Hematopoietic fate maps in the developing mouse embryo remain imprecise. Definitive, adult-type hematopoiesis first appears in the fetal liver, then progresses to the spleen and bone marrow. Clonogenic common lymphoid progenitors and clonogenic common myeloid progenitors (CMPs) in adult mouse bone marrow that give ris...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.3.627
更新日期:2001-08-01 00:00:00