当前位置： SCI文献检索 > BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS期刊下所有文献 > Crystallographic analysis reveals a unique conformation of the ADP-bound novel rice kinesin K16.

Crystallographic analysis reveals a unique conformation of the ADP-bound novel rice kinesin K16.

Abstract：

:Biochemical studies revealed that the novel rice plant-specific kinesin K16 has several unique enzymatic characteristics as compared to conventional kinesins. The ADP-free form of K16 is very stable, whereas the ADP-free form of conventional kinesins is labile. In the present study, the crystal structure of the novel rice kinesin motor domain (K16MD) complexed with Mg-ADP was determined at 2.4 Å resolutions. The overall structure of K16MD is similar to that of conventional kinesin motor domains, as expected from the high amino acid sequence similarity (43.2%). However, several unique structures in K16 were observed. The position and length of the L5, L11, and L12 loops, which are key functional regions, were different from those observed in conventional kinesins. Moreover, the neck-linker region of the ADP-bound K16MD showed an ordered conformation at a position quite different from that previously observed in conventional kinesins. These structural differences may reflect the unique enzymatic characteristics of rice kinesin K16.

journal_name

Biochem Biophys Res Commun

journal_title

Biochemical and biophysical research communications

authors

Tanaka K,Umeki N,Mitsui T,Fujimoto Z,Maruta S

doi

10.1016/j.bbrc.2010.09.043

subject

Has Abstract

pub_date

2010-10-15 00:00:00

pages

251-6

issue

2

eissn

0006-291X

issn

1090-2104

pii

S0006-291X(10)01727-4

journal_volume

401

pub_type

杂志文章

相关文献

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS文献大全
  • Multi-spectrometric analyses of lipoteichoic acids isolated from Lactobacillus plantarum.

    abstract：:Lipoteichoic acid is a major cell wall virulence factor of gram-positive bacteria. LTAs from various bacteria have differential immunostimulatory potentials due to heterogeneity in their structures. Although recent studies have demonstrated that LTA isolated from Lactobacillus plantarum (pLTA) has anti-inflammatory pr...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2011.03.107

    authors： Jang KS,Baik JE,Han SH,Chung DK,Kim BG

    更新日期：2011-04-22 00:00:00

  • Isolation and characterization of monoclonal antibodies against ribonuclease inhibitor.

    abstract：:Mouse monoclonal antibodies were generated against human ribonuclease inhibitor, an intracellular regulatory protein. A total of four antibodies were isolated, all of which were of the immunoglobulin G1 subtype. Western blot analysis of the antibodies suggested monospecificity. Based on immunoradiometric competition a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(88)80045-7

    authors： Feldman M,Kohtz DS,Kleinberg DL

    更新日期：1988-11-30 00:00:00

  • Reassignment of the human macrophage colony stimulating factor gene to chromosome 1p13-21.

    abstract：:Macrophage colony stimulating factor (CSF-1) is a member of a family of glycoproteins that are necessary for the normal proliferation and differentiation of myeloid progenitor cells. The human CSF-1 gene has previously been assigned to chromosome 5 using somatic cell hybrids, and further localized to 5q33 by in situ h...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(92)91850-p

    authors： Saltman DL,Dolganov GM,Hinton LM,Lovett M

    更新日期：1992-02-14 00:00:00

  • Insulin receptor substrates form high-molecular-mass complexes that modulate their availability to insulin/insulin-like growth factor-I receptor tyrosine kinases.

    abstract：:Insulin receptor substrates (IRSs) are phosphorylated by activated insulin/insulin-like growth factor (IGF)-I receptor tyrosine kinases. Phosphotyrosyl IRSs are recognized by signaling molecules possessing src homology region 2 (SH2) domains, which mediate various insulin/IGF bioactivities. However, we have shown that...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.12.045

    authors： Fukushima T,Arai T,Ariga-Nedachi M,Okajima H,Ooi Y,Iijima Y,Sone M,Cho Y,Ando Y,Kasahara K,Ozoe A,Yoshihara H,Chida K,Okada S,Kopchick JJ,Asano T,Hakuno F,Takahashi S

    更新日期：2011-01-21 00:00:00

  • In vivo assay of human NK-dependent ADCC using NOD/SCID/gammac(null) (NOG) mice.

    abstract：:Monoclonal antibodies are essential to the success of molecularly targeted therapies. Recently, numerous therapeutic antibodies have been developed for various diseases, including cancer and autoimmune diseases. Experimental systems to effectively evaluate these candidate antibodies are urgently needed. One of the mec...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.07.145

    authors： Shiokawa M,Takahashi T,Murakami A,Kita S,Ito M,Sugamura K,Ishii N

    更新日期：2010-09-03 00:00:00

  • Suppression of atherosclerosis by synthetic REV-ERB agonist.

    abstract：:The nuclear receptors for heme, REV-ERBα and REV-ERBβ, play important roles in the regulation of metabolism and inflammation. Recently it was demonstrated that reduced REV-ERBα expression in hematopoetic cells in LDL receptor null mice led to increased atherosclerosis. We sought to determine if synthetic REV-ERB agoni...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2015.03.070

    authors： Sitaula S,Billon C,Kamenecka TM,Solt LA,Burris TP

    更新日期：2015-05-08 00:00:00

  • CVD-associated non-coding RNA, ANRIL, modulates expression of atherogenic pathways in VSMC.

    abstract：:ANRIL is a newly discovered non-coding RNA lying on the strongest genetic susceptibility locus for cardiovascular disease (CVD) in the chromosome 9p21 region. Genome-wide association studies have been linking polymorphisms in this locus with CVD and several other major diseases such as diabetes and cancer. The role of...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.02.050

    authors： Congrains A,Kamide K,Katsuya T,Yasuda O,Oguro R,Yamamoto K,Ohishi M,Rakugi H

    更新日期：2012-03-23 00:00:00

  • Protein-binding DNA regions inside and in the vicinity of the cytochrome P450bm-3 gene from Bacillus megaterium.

    abstract：:Protein-binding DNA-regions inside and in the vicinity of the phenobarbital-inducible P450bm-3 gene from Bacillus megaterium were characterized by gel-retardation technique and foot-printing analysis. Regions with induction-dependent protein binding capacity were shown to be situated in -279bp/-215bp, -215bp/+83bp and...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1994.1123

    authors： Gaidamakova EK,Karginov VA,Kovalenko SP

    更新日期：1994-02-15 00:00:00

  • Corticotropin-releasing hormone activates protein kinase C in an isoenzyme-specific manner.

    abstract：:Protein kinase C (PKC) has recently emerged as mediator of corticotropin-releasing hormone (CRH) effects. Aim of the present study was to study the effects of CRH on each PKC isoenzyme. As a model we have used the PC12 rat pheochromocytoma cell line, expressing the CRH type 1 receptor (CRHR1). Our data were as follows...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.12.078

    authors： Dermitzaki E,Tsatsanis C,Charalampopoulos I,Androulidaki A,Alexaki VI,Castanas E,Gravanis A,Margioris AN

    更新日期：2005-02-18 00:00:00

  • Comparison of mucosal absorption-enhancing activity between a claudin-3/-4 binder and a broadly specific claudin binder.

    abstract：:Intercellular spaces between adjacent mucosal epithelial cells are sealed by tight junctions (TJs) that prevent the free movement of solutes across the epithelium. Claudins (CLs), a family of 27 integral membrane proteins, are essential components for TJ seals. We previously used a CL-3/-4 binder, the C-terminal fragm...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2012.05.060

    authors： Matsuhisa K,Kondoh M,Suzuki H,Yagi K

    更新日期：2012-06-29 00:00:00

  • Migration defects by DISC1 knockdown in C57BL/6, 129X1/SvJ, and ICR strains via in utero gene transfer and virus-mediated RNAi.

    abstract：:Disrupted-in-Schizophrenia 1 (DISC1) is a promising genetic risk factor for major mental disorders. Many groups repeatedly reported a role for DISC1 in brain development in various strains of mice and rats by using RNA interference (RNAi) approach. Nonetheless, due to the complexity of its molecular disposition, such ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2010.08.117

    authors： Kubo K,Tomita K,Uto A,Kuroda K,Seshadri S,Cohen J,Kaibuchi K,Kamiya A,Nakajima K

    更新日期：2010-10-01 00:00:00

  • Identification of ADAMTS13 peptide sequences binding to von Willebrand factor.

    abstract：:ADAMTS13 cleaves multimeric von Willebrand factor (VWF) to regulate VWF-mediated thrombus formation. To search ADAMTS13 peptide sequences binding to VWF, a lambda-phage library expressing various peptides of ADAMTS13 on the surface was screened using VWF either immobilized or in solution under static condition. By the...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2009.11.138

    authors： Moriki T,Maruyama IN,Igari A,Ikeda Y,Murata M

    更新日期：2010-01-01 00:00:00

  • Rad51-related changes in global gene expression.

    abstract：:High expression of Rad51, the catalytic component in homologous recombination, has been reported to contribute to genomic instability. To elucidate biological processes related to Rad51, we performed global gene expression analysis on human fibrosarcoma cells induced to express variable Rad51 levels. The results indic...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.12.185

    authors： Orre LM,Fält S,Szeles A,Lewensohn R,Wennborg A,Flygare J

    更新日期：2006-03-10 00:00:00

  • Purification of a novel eIF-2 alpha protein kinase from calf brain.

    abstract：:A new eukaryotic initiation factor 2 kinase has been purified for the first time from calf brain cytosol. The purification of a nonabundant novel protein kinase activity, designated as PKI, that phosphorylates the alpha subunit of eukaryotic initiation factor 2 is described. The protein kinase activity was assayed usi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/0006-291x(90)90998-3

    authors： Alcázar A,Méndez E,Martín ME,Salinas M

    更新日期：1990-02-14 00:00:00

  • Soluble PTK7 inhibits tube formation, migration, and invasion of endothelial cells and angiogenesis.

    abstract：:Human PTK7 is a defective receptor protein tyrosine kinase and its expression is upregulated in various cancers including colorectal carcinomas. To determine whether PTK7 functions in angiogenesis, we have expressed and purified the extracellular domain of PTK7 (soluble PTK7; sPTK7) as a decoy receptor to counteract t...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2008.04.168

    authors： Shin WS,Maeng YS,Jung JW,Min JK,Kwon YG,Lee ST

    更新日期：2008-07-11 00:00:00

  • Parathyroid hormone and parathyroid hormone-related peptide, and their receptors.

    abstract：:Parathyroid hormone (PTH) has a central role in the regulation of serum calcium and phosphate, while parathyroid hormone-related peptide (PTHrP) has important developmental roles. Both peptides signal through the same receptor, the PTH/PTHrP receptor (a class B G-protein-coupled receptor). The different biological eff...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章,评审

    doi：10.1016/j.bbrc.2004.11.069

    authors： Gensure RC,Gardella TJ,Jüppner H

    更新日期：2005-03-18 00:00:00

  • Multiple copies of beta-lactoglobulin promoter do not function as LCR.

    abstract：:Increasing the number of transcription factor binding sites within a construct can enhance expression. In an attempt to create a synthetic locus control region for mammary expression, we have generated beta-lactoglobulin-reporter constructs with multiple copies of the cluster of transcription sites normally located wi...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.2000.2766

    authors： James RM,Neil C,Webster J,Roos S,Clark AJ,Whitelaw CB

    更新日期：2000-05-27 00:00:00

  • Signal peptide protection by specific chaperone.

    abstract：:TorD is the private chaperone of TorA, a periplasmic respiratory molybdoenzyme of Escherichia coli. In this study, it is demonstrated that TorD is required to maintain the integrity of the twin-arginine signal sequence of the cytoplasmic TorA precursors. In the absence of TorD, 35 out of the 39 amino acid residues of ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2005.11.107

    authors： Genest O,Seduk F,Ilbert M,Méjean V,Iobbi-Nivol C

    更新日期：2006-01-20 00:00:00

  • GATA4 loss-of-function mutation underlies familial dilated cardiomyopathy.

    abstract：:The cardiac transcription factor GATA4 is essential for cardiac development, and mutations in this gene have been implicated in a wide variety of congenital heart diseases in both animal models and humans. However, whether mutated GATA4 predisposes to dilated cardiomyopathy (DCM) remains unknown. In this study, the wh...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2013.09.023

    authors： Li RG,Li L,Qiu XB,Yuan F,Xu L,Li X,Xu YJ,Jiang WF,Jiang JQ,Liu X,Fang WY,Zhang M,Peng LY,Qu XK,Yang YQ

    更新日期：2013-10-04 00:00:00

  • Tyrosine protein kinase activity in the DMBA-induced rat mammary tumor: inhibition by quercetin.

    abstract：:Tyrosine protein kinase activity was measured in membranes from DMBA-induced mammary tumors, with Angiotensin II as substrate. The apparent Km for the peptide was 3.3 mM. This enzymatic activity is inhibited by Ca+2; Mn+2 can replace Mg+2 with an increase in the Km for ATP from 47 /microM to 172 microM. The enzymatic ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(84)80264-8

    authors： Levy J,Teuerstein I,Marbach M,Radian S,Sharoni Y

    更新日期：1984-09-28 00:00:00

  • Design of a thrombin resistant human acidic fibroblast growth factor (hFGF1) variant that exhibits enhanced cell proliferation activity.

    abstract：:Acidic fibroblast growth factors (FGF1s) are heparin binding proteins that regulate a wide array of key cellular processes and are also candidates for promising biomedical applications. FGF1-based therapeutic applications are currently limited due to their inherent thermal instability and susceptibility to proteases. ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.08.029

    authors： Kerr R,Agrawal S,Maity S,Koppolu B,Jayanthi S,Suresh Kumar G,Gundampati RK,McNabb DS,Zaharoff DA,Kumar TKS

    更新日期：2019-10-15 00:00:00

  • Extracellular signal-regulated kinase (ERK) activity is required for TPA-mediated inhibition of drug-induced apoptosis.

    abstract：:Leukemia cells respond to toxic stimuli by undergoing a form of programmed cell death known as apoptosis. However, the signaling events responsible for the execution of this form of death are poorly understood. Mitogen-activated protein kinase (MAPK) signaling cascades are involved in the cellular response to extracel...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1998.8410

    authors： Stadheim TA,Kucera GL

    更新日期：1998-04-07 00:00:00

  • Co-culture of endothelial cells and patterned smooth muscle cells on titanium: construction with high density of endothelial cells and low density of smooth muscle cells.

    abstract：:Endothelialization has been considered a promising method to improve the biocompatibility of vascular implanted biomaterials. However, little is known about the anti-coagulation, anti-inflammatory, anti-atherosclerosis and anti-shedding property of the attached endothelial cells (ECs) and the relationship with their b...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2014.10.127

    authors： Li J,Zhang K,Wu J,Liao Y,Yang P,Huang N

    更新日期：2015-01-09 00:00:00

  • Calcium and SLY genes suppress the temperature-sensitive secretion defect of Saccharomyces cerevisiae uso1 mutant.

    abstract：:Saccharomyces cerevisiae uso1-1 mutant stops the transport of secretory proteins from the endoplasmic reticulum to the Golgi apparatus at 37 degrees C. We found that this temperature-sensitive defect was suppressed either by increasing the concentration of calcium ion in the medium or by introducing in the cell the SL...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1996.0459

    authors： Kito M,Seog DH,Igarashi K,Kambe-Honjo H,Yoda K,Yamasaki M

    更新日期：1996-03-27 00:00:00

  • Comprehensive investigation of aberrant microRNAs expression in cells culture model of MnCl2-induced neurodegenerative disease.

    abstract：:Manganese (Mn) is required in various human physiological processes. Excessive Mn exposure causes manganism, a progressive neurodegenerative disorder similar to idiopathic Parkinson's disease (IPD). However, the detailed mechanism of Mn-induced neurotoxicity is not yet fully understood. MicroRNAs (miRNAs) play importa...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2017.03.041

    authors： He R,Xie X,Lv L,Huang Y,Xia X,Chen X,Zhang L

    更新日期：2017-04-29 00:00:00

  • The molecular and functional characterization of E2F-5 transcription factor.

    abstract：:The E2F activity plays a critical role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. We describe here molecular cloning and functional characterization of a fifth member of the E2F family of transcription factors. E2F-...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1997.8010

    authors： Vaishnav YN,Vaishnav MY,Pant V

    更新日期：1998-01-26 00:00:00

  • Metabolism of d-glucose and its pentaacetate ester in muscles and pancreatic islets of GLUT4 null mice.

    abstract：:The metabolism of d-glucose and its pentaacetate ester was investigated in GLUT4 null mice and control C57Bl6/CBA mice. The incorporation of d-[U-(14)C]glucose (1.7 mM) into glycogen of diaphragm, soleus, and extensor digitorium longus muscles averaged, in the GLUT4 null mice, only 34 +/- 7% of the mean corresponding ...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1006/bbrc.1999.1591

    authors： Ladrière L,Charron MJ,Malaisse WJ

    更新日期：1999-11-02 00:00:00

  • Dichotomy in the laminin-binding properties of soluble and membrane-bound human galactoside-binding protein.

    abstract：:Recent studies indicate that galactoside-binding proteins may bind the poly-N-acetyllactosamine sequences of laminin. We questioned whether human galactoside-binding protein (hL-31) binds to laminin and whether cells that express hL-31 on their surface use it as a laminin receptor to promote cellular attachment. The d...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/s0006-291x(05)81601-8

    authors： Ochieng J,Gerold M,Raz A

    更新日期：1992-08-14 00:00:00

  • Severe Fanconi Anemia phenotypes in Fancd2 depletion mice.

    abstract：:Fanconi anemia (FA) is a genetic disorder characterized by congenital malfunction, bone marrow failure and hypersensitivity to DNA damage. FANCD2 protein play the central role in FA pathway. To study the in vivo role of FANCD2, we generated and characterized a new Fancd2 knockout mouse strain with 7bp deletion in Fanc...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2019.04.201

    authors： Yang Q,Xie H,Zhong Y,Li D,Ke X,Ying H,Yu B,Zhang T

    更新日期：2019-06-30 00:00:00

  • Delta12-Prostaglandin J2 inhibits the ubiquitin hydrolase UCH-L1 and elicits ubiquitin-protein aggregation without proteasome inhibition.

    abstract：:To investigate molecular mechanisms linking inflammation with neurodegeneration, we treated neuronal cultures with prostaglandins (PGs), which are mediators of inflammation. PGA1, D2, J2, and Delta12-PGJ2, but not PGE2, reduced the viability and raised the levels of ubiquitinated proteins in the neuronal cells. PGJ2 a...

    journal_title：Biochemical and biophysical research communications

    pub_type： 杂志文章

    doi：10.1016/j.bbrc.2004.05.098

    authors： Li Z,Melandri F,Berdo I,Jansen M,Hunter L,Wright S,Valbrun D,Figueiredo-Pereira ME

    更新日期：2004-07-09 00:00:00