Abstract:
BACKGROUND:EPLIN (epithelial protein lost in neoplasm) is a cytoskeletal associated protein involved in the regulation of actin dynamics and subsequently in cell motility. EPLIN expression is frequently reduced in a variety of cancer cells and tissues and this loss may account for increased invasive traits in cancer cells. The current study aimed to assess the role of EPLIN in endothelial cells and the angiogenic process. METHODS:EPLINα expression was enhanced in HECV endothelial cells through transfection with a pEF6 expression plasmid containing the full coding sequence of EPLINα. The impact of EPLINα on HECV cells was then assessed using a range of in vitro and in vivo models. RESULTS:transfection and over-expression of HECV cells with EPLINα expression plasmid resulted in a significant reduction in cell-matrix adhesion (P = 0.003), the rate of migration (P = 0.009) and tubule formation (P = 0.007) and also enhanced paxillin staining compared to transfection controls. Additionally, MDA-MB-231 breast cancer cells co-injected with HECV(EPLIN exp) cells developed at a slower rate than those co-injected with HECV(pEF6) cells (day 26, P = 0.05; day 33, P = 0.065, overall deviation over time P < 0.001). Treatment of HECV(EPLIN exp) cells with ERK inhibitor could rescue HECV cells from the inhibitory effect of EPLINα over-expression on tubule formation, returning these cells to control levels. CONCLUSIONS:EPLINα over-expression can regulate HECV cell motility, matrix adhesion and tubule formation in vitro and slow in vivo tumour formation, suggesting an anti-angiogenic role for EPLINα. Extracellular signal-regulated kinase (ERK) also appears to be linked to the ability of EPLINα to inhibit tubule formation in vitro.
journal_name
Angiogenesisjournal_title
Angiogenesisauthors
Sanders AJ,Ye L,Mason MD,Jiang WGdoi
10.1007/s10456-010-9188-7subject
Has Abstractpub_date
2010-12-01 00:00:00pages
317-26issue
4eissn
0969-6970issn
1573-7209journal_volume
13pub_type
杂志文章相关文献
ANGIOGENESIS文献大全abstract::Cardiovascular diseases (CVDs) are the leading causes of death worldwide. Increasing reports demonstrated that non-coding RNAs (ncRNAs) have been crucially involved in the development of CVDs. Piwi-interacting RNAs (piRNAs) are a novel cluster of small non-coding RNAs with strong uracil bias at the 5' end and 2'-O-met...
journal_title:Angiogenesis
pub_type: 杂志文章,评审
doi:10.1007/s10456-020-09750-w
更新日期:2020-10-04 00:00:00
abstract::The hst-1 gene product, one of the fibroblast growth factor family proteins, has transforming and angiogenic activities. The BALB/c 3T3 cell line was transfected with an expression vector harboring human hst-1 cDNA and the malignant properties of two stably transfected clones, TC-1 and TC-2, were examined. The stimula...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1023/a:1009288405970
更新日期:1998-01-01 00:00:00
abstract::The semaphorins and plexins comprise a family of cysteine-rich proteins implicated in control of nerve growth and development and regulation of the immune response. Our group and others have found that Semaphorin 4D (SEMA4D) and its receptor, Plexin-B1, play an important role in tumor-induced angiogenesis, with some n...
journal_title:Angiogenesis
pub_type: 杂志文章,收录出版
doi:10.1007/s10456-012-9268-y
更新日期:2012-09-01 00:00:00
abstract::Heparan sulfate (HS), a long linear polysaccharide of alternating disaccharide residues, interacts with a wide variety of proteins, including many angiogenic factors. The involvement of HS in signaling of pro-angiogenic factors (e.g. vascular endothelial growth factor and fibroblast growth factor 2), as well as intera...
journal_title:Angiogenesis
pub_type: 杂志文章,评审
doi:10.1007/s10456-013-9401-6
更新日期:2014-07-01 00:00:00
abstract::Vascular endothelial growth factor receptor 2 (VEGFR-2) and α v ß 3 integrin are the most frequently addressed targets in molecular imaging of tumor angiogenesis. In preclinical studies, molecular imaging of angiogenesis has shown potential to detect and differentiate benign and malignant lesions of the breast. Thus, ...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-016-9499-4
更新日期:2016-04-01 00:00:00
abstract::Regulator of calcineurin 1 (RCAN1) is an endogenous inhibitor of the calcineurin pathway in cells. It is expressed as two isoforms in vertebrates: RCAN1.1 is constitutively expressed in most tissues, whereas transcription of RCAN1.4 is induced by several stimuli that activate the calcineurin-NFAT pathway. RCAN1.4 is h...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-017-9542-0
更新日期:2017-08-01 00:00:00
abstract::Angiogenesis, or new blood vessel formation, is an important process in the pathogenesis of several diseases and thus has been targeted for the prevention and treatment for many disorders. However, the anti-angiogenic agents that are currently in use are mainly synthetic compounds and humanized monoclonal antibodies, ...
journal_title:Angiogenesis
pub_type: 杂志文章,评审
doi:10.1007/s10456-016-9512-y
更新日期:2016-07-01 00:00:00
abstract::noni, the juice of the fruit from the Morinda citrifolia plant, has been used for centuries as a medicinal agent. We tested the effects of noni juice in a three-dimensional fibrin clot matrix model using human placental vein and human breast tumor explants as sources for angiogenic vessel development. Noni in concentr...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1023/B:AGEN.0000011800.04507.4b
更新日期:2003-01-01 00:00:00
abstract:BACKGROUND:Vasculogenic mimicry (VM) is the formation of vascular channels by tumor cells or tumor cell-derived, trans-differentiated cells in highly aggressive, solid tumors. However, the disease features and prognostic value of VM for overall survival of cancer patients remain controversial. METHOD:To systematically...
journal_title:Angiogenesis
pub_type: 杂志文章,meta分析
doi:10.1007/s10456-016-9500-2
更新日期:2016-04-01 00:00:00
abstract::Endometriosis, defined as the presence of endometrial tissue outside the uterus, is an estrogen-dependent disease which causes pelvic pain and subfertility in women of reproductive age. The condition has a dramatic impact on the professional, social and marital life of sufferers. Direct and indirect evidence suggests ...
journal_title:Angiogenesis
pub_type: 杂志文章,评审
doi:10.1007/s10456-005-9005-x
更新日期:2005-01-01 00:00:00
abstract:BACKGROUND:In patients with resectable colorectal liver metastases (CRLM), distinct histopathological growth patterns (HGPs) develop at the interface between the tumour and surrounding tissue. The desmoplastic (d) HGP is characterised by angiogenesis and a peripheral fibrotic rim, whereas non-angiogenic HGPs co-opt end...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-019-09661-5
更新日期:2019-05-01 00:00:00
abstract:BACKGROUND:The term "intramuscular hemangioma capillary type" (IHCT) refers to a fast-flow vascular lesion that is classified as a tumor, although its phenotype overlaps with arteriovenous malformation (AVM). The purpose of this study was to identify somatic mutations in IHCT. METHODS:Affected tissue specimens were ob...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-019-09678-w
更新日期:2019-11-01 00:00:00
abstract::In this study we investigated the control of the angiogenic/haemorrhagic phenotype of oestrogen-induced rat pituitary tumours of an F1 hybrid (F1) of Fischer 344 (F344) (tumour susceptible) and Brown Norway (tumour resistant) strains. F1 forms a pituitary tumour upon chronic oestrogen treatment, but microvessel count ...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1023/a:1021550211921
更新日期:2002-01-01 00:00:00
abstract::Anti-VEGF (vascular endothelial growth factor) therapy with the monoclonal antibody bevacizumab can cause gastrointestinal (GI) perforations. In recent years it became apparent that GI perforations also occur during treatment with antiangiogenic tyrosine kinase inhibitors (TKIs). It is of clinical importance to consid...
journal_title:Angiogenesis
pub_type: 杂志文章,评审
doi:10.1007/s10456-010-9197-6
更新日期:2011-05-01 00:00:00
abstract::During prolonged hypoxic conditions, endothelial cells change their gene expression to adjust to the low oxygen environment. This process is mainly regulated by the hypoxia-inducible factors, HIF-1α and HIF-2α. Although endothelial cells do not form sprouts during prolonged hypoxic culturing, silencing of HIF-2α parti...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-016-9527-4
更新日期:2017-02-01 00:00:00
abstract::Angiogenesis is a complex process involving coordinated steps of endothelial cell activation, proliferation, migration, tube formation and capillary sprouting with participation of intracellular signaling pathways. Regulation of angiogenesis carries tremendous potential for cancer therapy. Our earlier studies showed t...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-005-9024-7
更新日期:2005-01-01 00:00:00
abstract::Transmembrane-4-L-six-family-1 (TM4SF1) is a tetraspanin-like membrane protein that is highly and selectively expressed by cultured endothelial cells (EC) and, in vivo, by EC lining angiogenic tumor blood vessels. TM4SF1 is necessary for the formation of unusually long (up to a 50 μm), thin (~100-300 nm wide), F-actin...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-011-9218-0
更新日期:2011-09-01 00:00:00
abstract::Inflammation in the normally immune-privileged cornea can initiate a pathologic angiogenic response causing vision-threatening corneal neovascularization. Inflammatory pathways, however, are numerous, complex and are activated in a time-dependent manner. Effective resolution of inflammation and associated angiogenesis...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-018-9604-y
更新日期:2018-05-01 00:00:00
abstract::Intermittent Hypoxia (IH) that develops in neovascularized solid tumours has been described to positively influence the tumour growth by modulating the behaviour of cancer cells as well as of endothelial cells. However, the molecular mechanisms regulated by IH still remain poorly understood. In this work, the effects ...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-009-9156-2
更新日期:2009-01-01 00:00:00
abstract::Endothelial cells are known to be a rich source of transcriptional gene expression. Recent technological advances now permit the detailed profiling of mRNA expression using arrays of known cDNAs on blots. We have used such arrays to examine expression of mRNA by primary isolates of human foreskin microvascular endothe...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1023/a:1009085422384
更新日期:1999-01-01 00:00:00
abstract::Antiangiogenic therapy, specially sorafenib, has become the standard of care for patients with advanced hepatocellular carcinoma (HCC), however, the improvement in survival time is not satisfactory. Previous studies have found that, in some circumstances, antiangiogenic therapy promoted tumor metastasis and the mechan...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-013-9357-6
更新日期:2013-10-01 00:00:00
abstract::Vascular anomalies can cause significant morbidity and mortality. Advances in diagnosis will be improved if noninvasive biomarkers can be identified, as obtaining a tissue biopsy can worsen the disease and precipitate complications. The goal of this study was to identify biomarkers for vascular anomaly patients to aid...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-016-9537-2
更新日期:2017-02-01 00:00:00
abstract::During embryonic development, the lymphatic system emerges by transdifferentiation from the cardinal vein. Although lymphatic and blood vasculature share a close molecular and developmental relationship, they display distinct features and functions. However, even after terminal differentiation, transitions between blo...
journal_title:Angiogenesis
pub_type: 临床试验,杂志文章
doi:10.1007/s10456-012-9264-2
更新日期:2012-06-01 00:00:00
abstract::Technology has caught up with retinal diseases of neovasculature. Work with anti-cancer, anti-angiogenic agents has fueled the way for ocular therapeutics. The market size for age-related macular degeneration and diabetic retinopathy is huge. Fifteen million people in the United States alone have age-related macular d...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-007-9068-y
更新日期:2007-01-01 00:00:00
abstract::Evidence has been gathered regarding the association between angiogenesis and inflammation in pathological situations. These two phenomena have long been coupled together in many chronic inflammatory disorders with distinct etiopathogenic origin, including psoriasis, rheumatoid arthritis, Crohn's disease, diabetes, an...
journal_title:Angiogenesis
pub_type: 杂志文章,评审
doi:10.1007/s10456-007-9074-0
更新日期:2007-01-01 00:00:00
abstract::Immunostaining with endothelial and pericyte markers was used to evaluate the cellular composition of angiogenic sprouts in several types of tumors and in the developing retina. Confocal microscopy revealed that, in addition to conventional endothelial tubes heavily invested by pericytes, all tissues contained small p...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1023/B:AGEN.0000021401.58039.a9
更新日期:2003-01-01 00:00:00
abstract::Rheumatoid arthritis (RA) is a chronic destructive musculo-skeletal disorder, associated with thickening of the synovial membrane lining the joints, inflammation and hyperproliferation of synovial cells, as well as a pro-inflammatory cytokine cascade, leukocyte infiltration, and tissue damage and bone resorption. An e...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1023/a:1009229508096
更新日期:1998-01-01 00:00:00
abstract::Receptor tyrosine kinase c-Kit and its ligand stem cell factor (SCF) regulate resident vascular wall cells and recruit circulating progenitors. We tested whether SCF may be induced by oxidized palmitoyl-arachidonoyl-phosphatidylcholine (OxPAPC) known to accumulate in atherosclerotic vessels. Gene expression analysis d...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-017-9590-5
更新日期:2018-05-01 00:00:00
abstract::Targeted ultrasound contrast imaging has the potential to become a reliable molecular imaging tool. A better understanding of the quantitative aspects of molecular ultrasound technology could facilitate the translation of this technique to the clinic for the purposes of assessing vascular pathology and detecting indiv...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-014-9447-0
更新日期:2015-01-01 00:00:00
abstract::Sunitinib is a potent and clinically approved tyrosine kinase inhibitor that can suppress tumour growth by inhibiting angiogenesis. However, conflicting data exist regarding the effects of this drug on the growth of metastases in preclinical models. Here we use 4T1 and RENCA tumour cells, which both form lung metastas...
journal_title:Angiogenesis
pub_type: 杂志文章
doi:10.1007/s10456-012-9291-z
更新日期:2012-12-01 00:00:00