Abstract:
:Silencing of tissue-specific gene expression in mammalian somatic cell hybrids is a well-documented epigenetic phenomenon which is both profound (involving a large number of genes) and enigmatic. Our aim was to utilize whole-genome microarray analyses to determine the true extent of gene silencing on a genomic level. By comparing gene expression profiles of hepatoma×fibroblast cell hybrids with those of parental cells, we have identified over 300 liver-enriched genes that are repressed at least 5-fold in the cell hybrids, the majority of which are repressed at least 10-fold. Also, we identify nearly 200 fibroblast-enriched genes that are repressed at least 5-fold. Silenced hepatic genes include several that encode transcription factors and proteins involved in signal transduction pathways. These data suggest that extensive reprogramming occurs in cell hybrids, leading to a nearly global (although not complete) loss of tissue-specific gene expression.
journal_name
Genomicsjournal_title
Genomicsauthors
Bulla GA,Luong Q,Shrestha S,Reeb S,Hickman Sdoi
10.1016/j.ygeno.2010.08.006subject
Has Abstractpub_date
2010-12-01 00:00:00pages
323-32issue
6eissn
0888-7543issn
1089-8646pii
S0888-7543(10)00181-3journal_volume
96pub_type
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