Abstract：
:To determine the influence of nutrients on the rate of biodegradation, a five-level, three-factor central composite design (CCD) was employed for bioremediation of seawater artificially contaminated with crude oil. Removal of total petroleum hydrocarbons (TPH) was the dependent variable. Samples were extracted and analyzed according to US-EPA protocols. A significant (R(2)=0.9645, P<0.0001) quadratic polynomial mathematical model was generated. Removal from samples not subjected to optimization and removal by natural attenuation were 53.3% and 22.6%, respectively. Numerical optimization was carried out based on desirability functions for maximum TPH removal. For an initial crude oil concentration of 1g/L supplemented with 190.21 mg/L nitrogen and 12.71 mg/L phosphorus, the Design-Expert software predicted 60.9% hydrocarbon removal; 58.6% removal was observed in a 28-day experiment.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zahed MA,Aziz HA,Isa MH,Mohajeri L,Mohajeri Sdoi
10.1016/j.biortech.2010.07.077subject
Has Abstractpub_date
2010-12-01 00:00:00pages
9455-60issue
24eissn
0960-8524issn
1873-2976pii
S0960-8524(10)01267-8journal_volume
101pub_type
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