Abstract:
AIMS:Percutaneous implantation has already been used clinically and is a great option for treating young patients. The use of autologous tissue-engineered valved stents might solve the problem of degeneration and limited durability of biological heart valves. METHODS AND RESULTS:Porcine pulmonary heart valves and small intestinal submucosa were obtained from a slaughterhouse. The intestinal submucosa was used to cover the inside of the porcine pulmonary valved stents. Endothelial cells (ECs) and autologous myofibroblasts (MFs) were used from carotid artery segments of juvenile sheep. After MF seeding, constructs were placed in a dynamic bioreactor system and cultured for 16 days. After additional EC seeding, tissue-engineered valved stents were percutaneously deployed into the annulus of the pulmonary valve (n = 9). Angiography was performed at implantation and 4-week follow-up. Constructs were analysed radiographically, by post-mortem examination, and microscopically. In all but one case, orthotopic positioning of the stents (n = 6) at the time of implantation and explantation was observed angiographically, macroscopically, and by computer tomography scan and demonstrated normal valve function (n = 7). Gross morphology confirmed excellent opening and closure characteristics of all leaflets after 4 weeks (n = 7). Strong expression of α-smooth muscle actin in neo-interstitial cells and of von Willebrand factor and PECAM-1 in ECs was revealed by immunocytochemistry. CONCLUSION:Good functioning and morphological characteristics were observed after percutaneous tissue-engineered valved stent implantation with autologous cells. This implantation of autologous tissue-engineered valved stents will become a valid future option in adolescents.
journal_name
Cardiovasc Resjournal_title
Cardiovascular researchauthors
Metzner A,Stock UA,Iino K,Fischer G,Huemme T,Boldt J,Braesen JH,Bein B,Renner J,Cremer J,Lutter Gdoi
10.1093/cvr/cvq212subject
Has Abstractpub_date
2010-12-01 00:00:00pages
453-61issue
3eissn
0008-6363issn
1755-3245pii
cvq212journal_volume
88pub_type
杂志文章abstract:AIM:Clinical use of amiodarone is associated with occasional development of torsade de pointes (TdP). However, preclinical models have failed to demonstrate the proarrhythmic potential of amiodarone. The objective of this study was to reveal and explain the pro- and anti-arrhythmic effects of acute exposure to amiodaro...
journal_title:Cardiovascular research
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更新日期:2002-02-15 00:00:00
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doi:10.1093/cvr/cvn171
更新日期:2008-10-01 00:00:00
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doi:
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doi:10.1093/cvr/cvw250
更新日期:2017-03-01 00:00:00
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更新日期:2006-01-01 00:00:00
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更新日期:2007-03-01 00:00:00
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更新日期:2014-06-01 00:00:00
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更新日期:1996-02-01 00:00:00
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更新日期:2015-07-01 00:00:00
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doi:10.1016/j.cardiores.2004.01.024
更新日期:2004-06-01 00:00:00
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doi:10.1093/cvr/27.10.1826
更新日期:1993-10-01 00:00:00
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更新日期:2008-11-01 00:00:00
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更新日期:2020-08-10 00:00:00
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pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
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更新日期:2003-11-01 00:00:00