Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes.

abstract：:Expanded bed adsorption chromatography is used to capture the protein product of interest from a crude biological suspension directly, thereby eliminating the need for the removal of the cell debris. While this technique may replace three or four unit operations in a typical downstream process for biological product r...

journal_title：Journal of biotechnology

authors： Balasundaram B,Harrison ST

更新日期：2008-02-01 00:00:00

abstract：:Combining the isochorismate synthase EntC and the chorismatase FkbO in a sequential enzyme cascade provides a useful system for the biocatalytic production and subsequent purification of isochorismate from an isochorismate/chorismate mixture. FkbO has a strict preference for chorismate - isochorismate is not accepted ...

journal_title：Journal of biotechnology

authors： Hubrich F,Müller M,Andexer JN

更新日期：2014-12-10 00:00:00

abstract：:A new, chemically defined medium providing dispersed growth and high biomass formation and a method for quantitative extraction of intracellular metabolites was used to investigate the cellular response of Streptomyces coelicolor A3(2) during growth and upon changes in nutrient utilization. Fast changes of the glucose...

journal_title：Journal of biotechnology

authors： Mira de Orduña R,Theobald U

更新日期：2000-02-17 00:00:00

abstract：:Experiments in the life sciences often involve tools from a variety of domains such as mass spectrometry, next generation sequencing, or image processing. Passing the data between those tools often involves complex scripts for controlling data flow, data transformation, and statistical analysis. Such scripts are not o...

journal_title：Journal of biotechnology

authors： Fillbrunn A,Dietz C,Pfeuffer J,Rahn R,Landrum GA,Berthold MR

更新日期：2017-11-10 00:00:00

abstract：:We previously reported that a recombinant strain, SB412(pLGM304), was constructed from acidomycin-resistant mutants of Serratia marcescens and produced 200 mg of d-biotin per liter of a medium containing sucrose and urea (Sakurai et al., 1993a, b). In the present work, we intended to improve the d-biotin production. B...

journal_title：Journal of biotechnology

authors： Sakurai N,Imai Y,Masuda M,Komatsubara S,Tosa T

更新日期：1994-07-29 00:00:00

abstract：:A computer system solution for integration of a distributed bioreactor monitoring and control instrumentation on the laboratory scale is described. Bioreactors equipped with on-line analyzers for mass spectrometry, near-infrared spectroscopy, electrochemical probes and multi-array gas sensors and their respective soft...

journal_title：Journal of biotechnology

authors： Cimander C,Bachinger T,Mandenius CF

更新日期：2003-08-15 00:00:00

abstract：:Microbial isolates from biofilters and petroleum-polluted bioremediation sites were screened for the presence of enantioselective epoxide hydrolases active towards tert-butyl glycidyl ether, benzyl glycidyl ether, and allyl glycidyl ether. Out of 270 isolated strains, which comprised bacteria, yeasts, and filamentous ...

journal_title：Journal of biotechnology

authors： Kotik M,Brichac J,Kyslík P

更新日期：2005-12-06 00:00:00

abstract：:We report molecular dynamic simulations showing that a DNA molecule constituted by five unique bases can be spontaneously inserted into single walled carbon nanotube (SWCNT) in normal conditions (P, T and water environment) depending on the tube radius value. The van der Waals and electrostatic interactions play a cen...

journal_title：Journal of biotechnology

authors： Clavier A,Kraszewski S,Ramseyer C,Picaud F

更新日期：2013-03-10 00:00:00

abstract：:Improving in vivo half-life and in vitro stability of protein-based therapeutics is a current challenge for the biopharmaceutical industry. In particular, recombinant human interferon alpha-2b (rhIFN-α2b), which belongs to a group of cytokines extensively used for the treatment of viral diseases and cancers, shows a p...

journal_title：Journal of biotechnology

authors： Ceaglio N,Gugliotta A,Tardivo MB,Cravero D,Etcheverrigaray M,Kratje R,Oggero M

更新日期：2016-03-10 00:00:00

abstract：:The role of oligosaccharides as biotic elicitors for the enhanced production of antibiotics and enzymes has been established in recent years. These findings could have significant promise for the pharmaceutical and biotechnology industries. Before the elicitors could be used at large-scale, reproducible production and...

journal_title：Journal of biotechnology

authors： Murphy T,Roy I,Harrop A,Dixon K,Keshavarz T

更新日期：2007-09-30 00:00:00

abstract：:A novel method that relies on the decoupling of the energy production and biosynthesis processes was used to characterise the maintenance, cell lysis and growth processes of Nitrosomonas sp. A Nitrosomonas culture was enriched in a sequencing batch reactor (SBR) with ammonium as the sole energy source. Fluorescent in ...

journal_title：Journal of biotechnology

authors： Vadivelu VM,Keller J,Yuan Z

更新日期：2006-11-10 00:00:00

abstract：:Mutants of transketolase (TK) with improved substrate specificity towards the non-natural aliphatic aldehyde substrate propionaldehyde have been obtained by directed evolution. We used the same active-site targeted saturation mutagenesis libraries from which we previously identified mutants with improved activity towa...

journal_title：Journal of biotechnology

authors： Hibbert EG,Senussi T,Smith ME,Costelloe SJ,Ward JM,Hailes HC,Dalby PA

更新日期：2008-04-30 00:00:00

abstract：:Supervision of batch bioprocess operations in real-time during the progress of a batch run offers many advantages over end-of-batch quality control. Multivariate statistical techniques such as multiway partial least squares (MPLS) provide an efficient modeling and supervision framework. A new type of MPLS modeling tec...

journal_title：Journal of biotechnology

authors： Undey C,Tatara E,Cinar A

更新日期：2004-02-19 00:00:00

abstract：:Based on the importance of heat shock proteins (HSPs) in diseases such as cancer, Alzheimer's disease or malaria, inhibitors of these chaperons are needed. Today's state-of-the-art techniques to identify HSP inhibitors are performed in microplate format, requiring large amounts of proteins and potential inhibitors. In...

journal_title：Journal of biotechnology

authors： Schax E,Walter JG,Märzhäuser H,Stahl F,Scheper T,Agard DA,Eichner S,Kirschning A,Zeilinger C

更新日期：2014-06-20 00:00:00

abstract：:An expression vector has been generated using a gene highly expressed under conditions found in a typical fed-batch bioreactor process. The ferritin heavy chain (HC) gene exhibits higher levels of expression in the late stages of a fed-batch bioreactor than in the early stages. This property was considered advantageou...

journal_title：Journal of biotechnology

authors： Prentice HL,Tonkin CJ,Caamano L,Sisk WP

更新日期：2007-01-30 00:00:00

abstract：:In the present investigation, a detailed biochemical analysis of the high H₂ producer D1 protein mutant strain L159I-N230Y of Chlamydomonas reinhardtii, carrying a double amino acid substitution, was made. The leucine residue L159 was replaced by isoleucine, and the N230 asparagine was replaced by tyrosine. The perfor...

journal_title：Journal of biotechnology

authors： Scoma A,Krawietz D,Faraloni C,Giannelli L,Happe T,Torzillo G

更新日期：2012-02-20 00:00:00

abstract：:Overcoming the effects of hydrolysate toxicity towards ethanologens is a key technical barrier in the biochemical conversion process for biomass feedstocks to ethanol. Despite its importance, the complexity of the hydrolysate toxicity phenomena and the lack of systematic studies, analysis and tools surrounding this is...

journal_title：Journal of biotechnology

authors： Franden MA,Pienkos PT,Zhang M

更新日期：2009-12-01 00:00:00

abstract：:This paper discusses the concept of generic model-based software sensors with particular reference to bioprocess application. The industrial need for software sensing is considered and two industrial bioprocess systems are used in order to highlight the operability problems which warrant their development. Alternative...

journal_title：Journal of biotechnology

authors： Montague GA,Morris AJ,Tham MT

更新日期：1992-08-01 00:00:00

abstract：:Hepatitis B virus, a member of the hepadnavirus family, causes the acute and chronic diseases of the human liver. The S domain of hepatitis B virus surface antigen (sHBsAg) was expressed under the control of the galactose-inducible GAL1 promoter in recombinant Saccharomyces cerevisiae. Batch fermentation of S. cerevis...

journal_title：Journal of biotechnology

authors： Kim EJ,Park YK,Lim HK,Park YC,Seo JH

更新日期：2009-05-20 00:00:00

abstract：:Preparations enriched by a specific protein are rarely easily obtained from natural host cells. Hence, recombinant protein production is frequently the sole applicable procedure. The ribosomal machinery, located in the cytoplasm is an outstanding catalyst of recombinant protein biosynthesis. Escherichia coli facilitat...

journal_title：Journal of biotechnology

authors： Sørensen HP,Mortensen KK

更新日期：2005-01-26 00:00:00

abstract：:In this study, we used the homologous recombination protein RecA to locate a specific sequence on DNA. Single-stranded (ss) DNA (80-mer, 5'-biotinylated), complementary to the sequence of interest, was labeled with quantum dots (Qdots(®)) via biotin-avidin binding. The DNA was then mixed with RecA to form a fluorescen...

journal_title：Journal of biotechnology

authors： Kimura Y,Goto Y,Oana H,Washizu M

更新日期：2012-12-15 00:00:00

abstract：:Chinese hamster ovary (CHO) cells are widely used for the production of biopharmaceuticals; however, our understanding of several physiological elements that contribute to productivity is limited. One of these is amino acid transport and how its limitation and/or regulation might affect productivity. To further our un...

journal_title：Journal of biotechnology

authors： Kyriakopoulos S,Polizzi KM,Kontoravdi C

更新日期：2013-12-01 00:00:00

abstract：:Based on the prototype experiment of treating herb wastewater by Up flow Anaerobic Sludge Bed and Anaerobic Filter reactor (UASBAF), an artificial neural network (ANN) model which adopts a back propagation algorithm with momentum and adaptive learning rate was established. And the effect of each parameter to the perfo...

journal_title：Journal of biotechnology

authors： Shi Y,Zhao XT,Zhang YM,Ren NQ

更新日期：2009-10-12 00:00:00

abstract：:Pseudomonas alcaligenes secretes a lipase with a high pH optimum, which has interesting properties for application in detergents. The expression of the lipase is strongly dependent on the presence of lipids in the growth medium such as soybean oil. The promoter of the gene was characterized and found to have resemblan...

journal_title：Journal of biotechnology

authors： Cox M,Gerritse G,Dankmeyer L,Quax WJ

更新日期：2001-03-09 00:00:00

abstract：:The spatiotemporal analysis of bioregulatory mechanisms at the level of intracellular multienzyme complexes and organelle interactions is made possible by the availability of endogenous and exogenous fluorescence probes, the development of microspectrofluorometers allowing one- and two-dimensional scans of intracellul...

journal_title：Journal of biotechnology

authors： Kohen E,Kohen C,Prince J,Schachtschabel DO,Hirschberg JG,Morliere P,Santus R,Dubertret L,Shapiro BL

更新日期：1990-01-01 00:00:00

abstract：:The polysaccharide xanthan which is produced by the γ-proteobacterium Xanthomonas campestris is used as a food thickening agent and rheologic modifier in numerous food, cosmetics and technical applications. Its great commercial importance stimulated biotechnological approaches to optimize the xanthan production. By ta...

journal_title：Journal of biotechnology

authors： Teckentrup J,Al-Hammood O,Steffens T,Bednarz H,Walhorn V,Niehaus K,Anselmetti D

更新日期：2017-09-10 00:00:00

abstract：:The purification of human antibodies in an aqueous two-phase system (ATPS) composed of polyethylene glycol (PEG) 6000 and phosphate was optimised by surface response methodology. A central composite design was used to evaluate the influence of phosphate, PEG and NaCl concentration and of the pH on the purity and extra...

journal_title：Journal of biotechnology

authors： Azevedo AM,Rosa PA,Ferreira IF,Aires-Barros MR

更新日期：2007-10-31 00:00:00

abstract：:"CloneAssistant 1.0" is a stand-alone software compatible with the current Windows operating systems, which can automatically design cloning primers with full consideration of the sequence information of vectors and genes, cloning strategies, the principles of primer design, reading frames, position effects, and enzym...

journal_title：Journal of biotechnology

authors： Shao C,Meng Y,Lv S,Zhong W,Wang Z,Chen M

更新日期：2010-11-01 00:00:00

abstract：:A repeat-batch membrane bioreactor was constructed for the novel bioconversion of isopropyl hexadecanoate to isopropyl cis-6-hexadecenoate by a Rhodococcus mutant. The addition of glutamate, thiamine, and MgSO(4) was very effective in improving not only the rate and yield of the bioconversion but also the maintenance ...

journal_title：Journal of biotechnology

authors： Koike K,Takeuchi K,Mino H,Takaiwa M,Tohoh T,Tadokoro T,Tsutoh K,Ito S

更新日期：2000-06-23 00:00:00

abstract：:In the current study, a three-tiered mutagenesis strategy was employed to simultaneously improve the thermostability and activity of halohydrin dehalogenase from Agrobacterium radiobacter AD1 (HheC) by engineering the last ten amino acids (Met245∼Glu254) of its C-terminal region. Initially, truncated mutagenesis resul...

journal_title：Journal of biotechnology

authors： Wang X,Han S,Yang Z,Tang L

更新日期：2015-10-20 00:00:00