KISS1 is down-regulated by 17beta-estradiol in MDA-MB-231 cells through a nonclassical mechanism and loss of ribonucleic acid polymerase II binding at the proximal promoter.

abstract：:In mammals, the brain-specific protein munc18-1 regulates synaptic vesicle exocytosis at the synaptic junction, in a step before vesicle fusion. We hypothesize that the rate of biosynthesis of munc18-1 messenger RNA (mRNA) and the amount of munc18-1 present in neurons and neuroendocrine cells are related to the physio...

journal_title：Endocrinology

authors： Kolk SM,Berghs CA,Vaudry H,Verhage M,Roubos EW

更新日期：2001-05-01 00:00:00

abstract：:Homogenates from male ddy mouse sublingual glands were incubated with 100 nM [125I]rT3 in the presence of 2 mM dithiothreitol (DTT). Metabolites were analyzed by HPLC or Dowex-50 minicolumn assay. 3,3'-Diiodothyronine and I- were the only appreciable products. Iodide release from rT3 was compatible with the enzymatic ...

journal_title：Endocrinology

authors： Tanaka K,Imura H

更新日期：1993-03-01 00:00:00

abstract：:Corticotropin-releasing factor receptor type 2beta, expressed in the rodent cardiovascular system, is a member of the G protein-coupled receptor family. This receptor is coupled positively to adenylate cyclase and is bound preferentially by the urocortin (Ucn)-related peptides (Uncs): Ucn, Ucn II, and Ucn III. In the ...

journal_title：Endocrinology

authors： Kageyama K,Suda T

更新日期：2003-06-01 00:00:00

abstract：:While uterine stromal cells (USC) appear to modify the function of uterine epithelial cells (UEC) under certain conditions in vivo, relatively little is known about the effect of epithelial cells on stromal cell differentiation and function. To determine if UEC modulate USC function in vitro, highly enriched (> 95%) c...

journal_title：Endocrinology

authors： Wegner CC,Carson DD

更新日期：1992-12-01 00:00:00

abstract：:Inflammation can interfere with endometrial receptivity. We examined how interleukin 1β (IL-1β) affects expression of the uterine gap junction protein connexin 43 (Cx43), which is known to be critical for embryonic implantation. We used an in vitro model of human endometrial stromal cells (ESCs), Western blotting, and...

journal_title：Endocrinology

authors： Yu J,Berga SL,Zou W,Yook DG,Pan JC,Andrade AA,Zhao L,Sidell N,Bagchi IC,Bagchi MK,Taylor RN

更新日期：2017-12-01 00:00:00

abstract：:CRF dose-dependently enhanced corticosterone (B) secretion by rat adrenal slices including both cortex and medulla. Conversely, CRF did not exert any B response by fragments of adrenocortical autotransplants, which are completely deprived of chromaffin tissue. However, autotransplant quarters exhibited a dose-dependen...

journal_title：Endocrinology

authors： Andreis PG,Neri G,Mazzocchi G,Musajo F,Nussdorfer GG

更新日期：1992-07-01 00:00:00

abstract：:The role of PRL in mammalian salt and water balance remains controversial. To avoid the methodological problems of exogenous PRL administration, endogenous hyperprolactinemia was established in normal rats by implantation of extra anterior pituitary glands under the kidney capsule. To eliminate excess glucocorticoid s...

journal_title：Endocrinology

authors： Adler RA,Herzberg VL,Brinck-Johnsen T,Sokol HW

更新日期：1986-04-01 00:00:00

abstract：:Levels of bovine placental lactogen (bPL) have been measured in the serum of dairy and beef cattle and in the milk and amniotic fluid of pregnant animals with a highly specific radioimmunoassay. In both dairy and beef cows, serum bPL levels remain low (less than 50 ng/ml) during the first two trimesters and then rise ...

journal_title：Endocrinology

authors： Bolander FF,Ulberg LC,Fellows RE

更新日期：1976-11-01 00:00:00

abstract：:Elevated activin A levels in inhibin-deficient mice promote the development of gonadal tumors and induce cachexia by reducing muscle, liver, stomach, and fat mass. Because activin A is an important regulator of tissue growth, inhibiting the actions of this TGFβ family ligand may halt or reverse pathology in diseased t...

journal_title：Endocrinology

authors： Makanji Y,Walton KL,Chan KL,Gregorevic P,Robertson DM,Harrison CA

更新日期：2011-10-01 00:00:00

abstract：:Obesity induced by high-fat (HF) feeding is associated with low-grade inflammation in peripheral tissues that predisposes to insulin resistance. Recent evidence suggests the occurrence of a similar process in the hypothalamus, which favors weight gain through impairment of leptin and insulin signaling. In addition to ...

journal_title：Endocrinology

authors： Thaler JP,Schwartz MW

更新日期：2010-09-01 00:00:00

abstract：:PTH stimulates synthesis and secretion of 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] in renal proximal tubule cells through activation of the protein kinase-A (PKA) or the protein kinase-C (PKC) signaling pathway. The relative contribution of the two transducing systems was explored using PTH fragments with selective act...

journal_title：Endocrinology

authors： Janulis M,Wong MS,Favus MJ

更新日期：1993-08-01 00:00:00

abstract：:We have demonstrated previously that both rat and human liver microsomes contain a highly specific binding protein for the anabolic steroids stanozolol (ST) and danazol (DA). In this study we solubilized the male rat liver ST-binding protein (STBP) and investigated the following parameters: 1) pharmacological properti...

journal_title：Endocrinology

authors： Luzardo OP,Machín RP,Díaz-Chico BN,Fernández L

更新日期：2000-09-01 00:00:00

abstract：:Recent evidence indicates that endogenous GnRH is required for maintenance of its own pituitary receptors (GnRH-R). We have measured GnRH-R in pituitaries of hypogonadotrophic hypogonadal (hpg) mice, in whom hypothalamic GnRH is deficient or absent. The GnRH-R concentration in hpg male mouse pituitaries was 10.6 +/- 1...

journal_title：Endocrinology

authors： Young LS,Speight A,Charlton HM,Clayton RN

更新日期：1983-07-01 00:00:00

abstract：:Epidermal growth factor (EGF) and TGFalpha are well known for their activities in the ovary. Both factors initiate signal transduction by binding to the common EGF receptor that has been demonstrated in the ovary across vertebrates from fish to humans. Using zebrafish as the model, we demonstrated in the present study...

journal_title：Endocrinology

authors： Pang Y,Ge W

更新日期：2002-01-01 00:00:00

abstract：:Aprotinin caused a dose-dependent inhibition of [125I]hCG binding to its receptor in plasma membranes prepared from luteinized rat ovaries. Displacement of [125I]hCG from its receptor by aprotinin was temperature dependent, with an ID50 of 300 microM at 4 C and an ID50 of 3700 microM at 22 C. Equilibrium binding data ...

journal_title：Endocrinology

authors： Wilks JW,Hui OJ

更新日期：1987-03-01 00:00:00

abstract：:To determine the relative role of the androgenic and/or estrogenic components of the action of dehydroepiandrosterone (DHEA) on the histomorphology and structure of the rat mammary gland, ovariectomized (OVX) female animals received DHEA administered alone or in combination with the pure antiandrogen flutamide or the ...

journal_title：Endocrinology

authors： Sourla A,Martel C,Labrie C,Labrie F

更新日期：1998-02-01 00:00:00

abstract：:PTH secretion is tightly regulated by extracellular calcium ([Ca2+]e) and in turn regulates calcium homeostasis through its action on target tissues. We investigated the mechanism and physiological significance of intracellular calcium ([Ca2+]i) levels in relation to the secretion of PTH in single bovine parathyroid c...

journal_title：Endocrinology

authors： Miki H,Maercklein PB,Fitzpatrick LA

更新日期：1995-07-01 00:00:00

abstract：:Aging of the female reproductive system in rats is marked by discrete stages in the disappearance of regular estrous cyclicity. We determined at which point in the transition to acyclicity changes in the LH pulse generator could be detected by comparing pulsatile LH release in ovariectomized young rats to three groups...

journal_title：Endocrinology

authors： Scarbrough K,Wise PM

更新日期：1990-02-01 00:00:00

abstract：:Thyroid toxicity of iodide excess has been demonstrated in animals fed with an iodide-rich diet; in vitro iodide is cytotoxic, inhibits cell growth, and induces morphological changes in thyroid cells of some species. In this study, we investigated the effect of iodide excess in an immortalized thyroid cell line (TAD-2...

journal_title：Endocrinology

authors： Vitale M,Di Matola T,D'Ascoli F,Salzano S,Bogazzi F,Fenzi G,Martino E,Rossi G

更新日期：2000-02-01 00:00:00

abstract：:Polypeptide YY(3-36) (PYY(3-36)) is a gastrointestinal secreted molecule, agonist of neuropeptide Y (NPY) receptor subtypes Y2 and Y5, recently involved in the control of food intake. Notably, several factors with key roles in energy homeostasis conduct pleiotropic effects upon the reproductive axis. However, whether ...

journal_title：Endocrinology

authors： Fernandez-Fernandez R,Aguilar E,Tena-Sempere M,Pinilla L

更新日期：2005-03-01 00:00:00

abstract：:The effects of leptin on the levels of CRF messenger RNA (mRNA) in the paraventricular hypothalamic nucleus (PVN), on the activation of the PVN CRF cells, and on the plasma levels of corticosterone were investigated in lean (+/?) and obese (ob/ob) C57BL/6J male mice. Murine leptin was s.c. infused using osmotic minipu...

journal_title：Endocrinology

authors： Huang Q,Rivest R,Richard D

更新日期：1998-04-01 00:00:00

abstract：:The role of the paraventricular nucleus-dorsal anterior hypothalamus (PVN-DAHA) in the control of anterior pituitary gland secretion of FSH and LH in castrated male and female rats was examined. Bilateral radiofrequency lesions of the PVN-DAHA in chronically ovariectomized (OVX) rats lowered plasma FSH levels by 33% (...

journal_title：Endocrinology

authors： Lumpkin MD,McCann SM

更新日期：1984-12-01 00:00:00

abstract：:We report here the effects of antibodies to estrogen receptor (ER) on the binding of tritiated 5 alpha-androstane-3 beta-17 beta-diol and antiestrogens to receptor in calf uterine cytosol. The antibodies, prepared from a rabbit immunized with purified estradiol-receptor complex from calf uterine nuclei, were found to ...

journal_title：Endocrinology

authors： Garcia M,Greene G,Rochefort H,Jensen EV

更新日期：1982-04-01 00:00:00

abstract：:We have investigated the role of protein kinase C (PKC) isoenzymes in the differential growth regulation of human pancreatic carcinoma cell lines by all-trans retinoic acid (RA). RA treatment results in dose-dependent stimulation of anchorage-independent growth in AsPc1 cells and growth inhibition in Capan 2 cells. Bo...

journal_title：Endocrinology

authors： Rosewicz S,Brembeck F,Kaiser A,Marschall ZV,Riecken EO

更新日期：1996-08-01 00:00:00

abstract：:GH binding proteins (GHBPs) are present in the blood of several species and by complexing with circulating GH may alter its clearance and distribution. The actions of human GHBP (hGHBP) have hitherto been studied indirectly via their effects on the clearance of hGH. In the present experiments, recombinant preparations...

journal_title：Endocrinology

authors： Fairhall KM,Carmignac DF,Robinson IC

更新日期：1992-10-01 00:00:00

abstract：:In the present study we investigated whether fetal exposure to flutamide affected messenger and protein levels of claudin-11, a key Sertoli cell factor in the establishment of the hemotesticular barrier, at the time of two key events of postnatal testis development: 1) before puberty (postnatal d 14) during the establ...

journal_title：Endocrinology

authors： Florin A,Maire M,Bozec A,Hellani A,Chater S,Bars R,Chuzel F,Benahmed M

更新日期：2005-03-01 00:00:00

abstract：:IGF-II has been reported to decrease neonatal islet cell apoptosis and in vitro adult islet cell necrosis and apoptosis, but the usefulness of IGF-II in a transplantation setting is unknown. We evaluated the effect of in vitro IGF-II incubations on microencapsulated rat islet survival both in vitro and in minimal mass...

journal_title：Endocrinology

authors： Robitaille R,Dusseault J,Henley N,Rosenberg L,Hallé JP

更新日期：2003-07-01 00:00:00

abstract：:Effect of cytosolic 3,5,3'-triiodo-L-thyronine (T3)-binding protein (CTBP) on [125I]T3 binding to nuclei was investigated in vitro. CTBP and nuclei were prepared from rat kidney. CTBP was inactivated by incubating with charcoal and activated by incubating with NADPH or with NADP and dithiothreitol. Two complexes of CT...

journal_title：Endocrinology

authors： Hashizume K,Miyamoto T,Kobayashi M,Suzuki S,Ichikawa K,Yamauchi K,Ohtsuka H,Takeda T

更新日期：1989-06-01 00:00:00

abstract：:To investigate the participation of cyclic nucleotides in LHRH-mediated gonadotropin release, cells from the anterior pituitaries of 15-day-old female rats were fractionated on an albumin gradient by sedimentation at unit gravity. gonadotrophs and somatotrophs were detected in the fractions by histology and RIA of hor...

journal_title：Endocrinology

authors： Snyder G,Naor Z,Fawcett CP,McCann SM

更新日期：1980-11-01 00:00:00

abstract：:Enlargement of the chimeric ventral prostate gland (VP) was induced by directly implanting either fetal urogenital sinus mesenchyme (UGM) or intact fetal urogenital sinus (UGS) into the VP of intact adult rats. The macromolecular content in the chimeric prostate increased from 40-100% (UGM implants) to 200-300% (UGS i...

journal_title：Endocrinology

authors： Thompson TC,Chung LW

更新日期：1986-06-01 00:00:00