Single-spin density-gradient ultracentrifugation vs gradient gel electrophoresis: two methods for detecting low-density-lipoprotein heterogeneity compared.

abstract：BACKGROUND:The antiphospholipid syndrome (APS) is an important cause of acquired thromboembolic complications and pregnancy morbidity. Its diagnosis is based on clinical and laboratory criteria, defined by strict guidelines. The original clinical and laboratory criteria for the identification of APS patients were publi...

journal_title：Clinical chemistry

authors： Devreese K,Hoylaerts MF

更新日期：2010-06-01 00:00:00

abstract：BACKGROUND:Measurement of purine and pyrimidine metabolites presents complex problems for separations currently performed by HPLC and thin-layer chromatography in clinical practice. We developed a novel capillary electrophoresis method for this purpose. METHODS:Separations were performed in 60 mmol/L borate-2-amino-2-...

journal_title：Clinical chemistry

authors： Adam T,Friedecký D,Fairbanks LD,Sevcík J,Barták P

更新日期：1999-12-01 00:00:00

abstract：:A potential genetic marker for hypertriglyceridemia and coronary disease, an apolipoprotein-CIII gene allele displaying a single nucleotide substitution, was found in 71% of Arabs with primary hypertriglyceridemia. Over 96% of the unrelated adults possessing this allele showed hypertriglyceridemia. The same allele was...

journal_title：Clinical chemistry

authors： Taş S

更新日期：1989-02-01 00:00:00

abstract：BACKGROUND:Commercial immunoassays for testosterone (Te) may give inaccurate results for samples from women and children, leading to misdiagnosis and inappropriate treatment. We developed a sensitive and specific tandem mass spectrometric assay for measurement of Te at the concentrations encountered in women and childr...

journal_title：Clinical chemistry

authors： Kushnir MM,Rockwood AL,Roberts WL,Pattison EG,Bunker AM,Fitzgerald RL,Meikle AW

更新日期：2006-01-01 00:00:00

abstract：:We compared a selection of quantitative immunological methods for prostatic acid phosphatase (PAP) with routine colorimetric assays for total and tartrate-labile acid phosphatase and evaluated their relative clinical merits in the differential diagnosis of prostatic carcinoma. We also assessed a wide range of commerci...

journal_title：Clinical chemistry

authors： Gibb I,Wilson LA,Powell PH,Tarbit I,Pratt D,Hall RR

更新日期：1986-09-01 00:00:00

abstract：BACKGROUND:Extracellular matrix alterations are important elements in the arterial changes seen in diabetes, being associated with increased vascular stiffness and the development of cardiovascular diseases. However, no biomarkers for diabetes-related arterial changes have been defined. METHODS:Mammary artery specimen...

journal_title：Clinical chemistry

authors： Cangemi C,Skov V,Poulsen MK,Funder J,Twal WO,Gall MA,Hjortdal V,Jespersen ML,Kruse TA,Aagard J,Parving HH,Knudsen S,Høilund-Carlsen PF,Rossing P,Henriksen JE,Argraves WS,Rasmussen LM

更新日期：2011-11-01 00:00:00

abstract：:The main developments in the "ligand assay" field in which I have been involved are traced. These include the original development of "first generation" competitive assays relying on radiolabeled analyte markers; the development of the first "second generation", noncompetitive (ultrasensitive) methods, which rely on t...

journal_title：Clinical chemistry

authors： Ekins RP

更新日期：1998-09-01 00:00:00

abstract：:Discrepancies among different methods for assaying human growth hormone have been described in various studies. The two major sources of discordant results are the heterogeneity of the antibodies and the different standardization bases used by the assay manufacturers. We propose standardizing assays with 22-kDa biosyn...

journal_title：Clinical chemistry

authors： Banfi G,Marinelli M,Pontillo M,Bonini P

更新日期：1992-10-01 00:00:00

abstract：:A competitive solid-phase fluoroenzymeimmunoassay has been developed for rapidly measuring 5 beta-pregnane-3 alpha, 20 alpha-diol-3 alpha-glucuronide (Pd-3G) directly in diluted specimens of pre-breakfast urine. The assay involves use of an antiserum to Pd-3G and enzyme-labeled antigen prepared by chemically linking g...

journal_title：Clinical chemistry

authors： Shah H,Saranko AM,Härkönen M,Adlercreutz H

更新日期：1984-02-01 00:00:00

abstract：BACKGROUND:Lead is an environmental pollutant, and human exposure is assessed by monitoring lead concentrations in blood. Because the main source of environmental exposure has been the use of leaded gasoline, its phase-out has led to decreased lead concentrations in the general population. Therefore, validated analytic...

journal_title：Clinical chemistry

authors： Patriarca M,Castelli M,Corsetti F,Menditto A

更新日期：2004-08-01 00:00:00

abstract：BACKGROUND:An accurate determination of the major HFE mutation (C282Y), which is associated with hereditary hemochromatosis, is important in diagnosis and risk assessment for this disease. We report a single-tube high-throughput PCR method for the detection of C282Y. METHODS:We combined three previously described prin...

journal_title：Clinical chemistry

authors： Donohoe GG,Laaksonen M,Pulkki K,Rönnemaa T,Kairisto V

更新日期：2000-10-01 00:00:00

abstract：:We describe use of near-infrared reflectance spectroscopy (NIRRS) to estimate the amount of fat in feces, for diagnosis of steatorrhea. After sample homogenization, the spectrum of the fecal homogenate is scanned over the near-infrared region. Assay of 94 samples of feces having a known concentration of fat showed the...

journal_title：Clinical chemistry

authors： Koumantakis G,Radcliff FJ

更新日期：1987-04-01 00:00:00

abstract：BACKGROUND:Cardiac troponin T (cTnT) is the preferred biomarker for the diagnosis of acute myocardial infarction (AMI). It has been suggested that cTnT is present predominantly in fragmented forms in human serum following AMI. In this study, we have used a targeted mass spectrometry assay and epitope mapping using West...

journal_title：Clinical chemistry

authors： Streng AS,de Boer D,van Doorn WP,Bouwman FG,Mariman EC,Bekers O,van Dieijen-Visser MP,Wodzig WK

更新日期：2017-02-01 00:00:00

abstract：:Optimal reaction conditions for assaying human lactate dehydrogenase pyruvate-to-lactate were determined for isoenzymes 1 and 5 at 25, 30, and 37 degrees C. Three of the nine different buffers examined--imidazole, triethanolamine, and N-tris(hydroxymethyl)-methyl-2-aminoethane sulfonic acid--are satisfactory. Beta-NAD...

journal_title：Clinical chemistry

authors： Buhl SN,Jackson KY,Graffunder B

更新日期：1978-02-01 00:00:00

abstract：:Of 98 patients' specimens examined for alkaline phosphatase (EC 3.1.3.1) isoenzymes by electrophoresis on cellulose acetate membrane after incubation with neuraminidase, 50 showed only a single liver or bone isoenzyme staining band; in 15 of these, the tissue origin of the fraction could not be accurately identified f...

journal_title：Clinical chemistry

authors： Rosalki SB,Foo AY

更新日期：1985-07-01 00:00:00

abstract：:We evaluated a new EMIT II monoclonal amphetamine/methamphetamine assay for screening human urine by comparing it with the EMIT d.a.u. monoclonal amphetamine/methamphetamine assay and a fluorescence polarization assay. The EMIT II assay has a cutoff of 1 mg/L d-methamphetamine. The EMIT II and EMIT d.a.u. assays were ...

journal_title：Clinical chemistry

authors： Dasgupta A,Saldana S,Kinnaman G,Smith M,Johansen K

更新日期：1993-01-01 00:00:00

abstract：BACKGROUND:There are 45 known genetic diseases that impair the lysosomal degradation of macromolecules. The loss of a single lysosomal hydrolase leads to the accumulation of its undegraded substrates in tissues and increases of related glycoconjugates in urine, some of which can be detected by screening of free oligosa...

journal_title：Clinical chemistry

authors： Xia B,Asif G,Arthur L,Pervaiz MA,Li X,Liu R,Cummings RD,He M

更新日期：2013-09-01 00:00:00

abstract：BACKGROUND:Rapid detection of biological threat agents is critical for timely therapeutic administration. Fluorogenic PCR provides a rapid, sensitive, and specific tool for molecular identification of these agents. We compared the performance of assays for 7 biological threat agents on the Idaho Technology, Inc. R.A.P....

journal_title：Clinical chemistry

authors： Christensen DR,Hartman LJ,Loveless BM,Frye MS,Shipley MA,Bridge DL,Richards MJ,Kaplan RS,Garrison J,Baldwin CD,Kulesh DA,Norwood DA

更新日期：2006-01-01 00:00:00

abstract：BACKGROUND:Associations between dairy intake and body composition and cardiometabolic traits have been inconsistently observed in epidemiological studies, and the causal relationship remains ill-defined. METHODS:We performed Mendelian randomization analysis using an established genetic variant located upstream of the ...

journal_title：Clinical chemistry

authors： Mendelian Randomization of Dairy Consumption Working Group.,CHARGE consortium.

更新日期：2019-06-01 00:00:00

abstract：BACKGROUND:Testing for troponin has important clinical value for patients who present with typical symptoms of acute coronary syndromes (ACS) such as chest pain (CP). Much less is known about the value of troponin testing for patients who present with other symptoms of ACS (anginal equivalent symptoms). METHODS:The ut...

journal_title：Clinical chemistry

authors： Ritter D,Lee PA,Taylor JF,Hsu L,Cohen JD,Chung HD,Virgo KS

更新日期：2004-01-01 00:00:00

abstract：:Diminished ability to utilize acetate (acetate intolerance) developed in a male patient on chronic hemodialysis after five years of maintenance dialysis. His ability to utilize lactate was also subnormal. We studied acetate metabolism in vitro by isolating lymphocytes from the patient's blood before dialysis and measu...

journal_title：Clinical chemistry

authors： Egan JD,Wells IC

更新日期：1979-03-01 00:00:00

abstract：:We evaluated a new, simple, enzymatic kinetic method from Wako Chemicals GmbH in comparison with a kinetic Jaffé method by using isotope dilution-mass spectrometry (ID-MS) as a reference method. An ID-MS-calibrated serum standard was used. Both the enzymatic and the Jaffé method correlated well with ID-MS, except for ...

journal_title：Clinical chemistry

authors： Lindbäck B,Bergman A

更新日期：1989-05-01 00:00:00

abstract：BACKGROUND:Late enhancement MRI (LE-MRI) and cardiac troponin I (cTnI) are sensitive methods to detect subclinical myocardial injury. We sought to investigate the relation between plasma concentrations of cTnI measured with a high-sensitivity assay (hs-cTnI) and the development of unrecognized myocardial infarctions (U...

journal_title：Clinical chemistry

authors： Barbier CE,Themudo R,Bjerner T,Johansson L,Lindahl B,Venge P,Lind L,Ahlström H

更新日期：2014-10-01 00:00:00

abstract：:We describe the production and characterization of a high-affinity monoclonal antibody, HB-22, for apolipoprotein (apo) A-I, a major protein of human high-density lipoproteins (HDL). Including Tween 20 in the reaction mixture increased the binding capacity of HB-22 to apo A-I. HB-22 showed monospecific reactivity with...

journal_title：Clinical chemistry

authors： Rothwell TC,Kamanna VS,Jin FY,Koren E,Foley T,Kashyap ML

更新日期：1995-08-01 00:00:00

abstract：:Antithrombin III (AT III) activity was determined with two different new chromogenic substances--Chromozym-TH (Tos-Gly-Arg-p-nitroanilide; Boehringer Mannheim) and alpha-N-carbobenzyl-oxy-L-lysine-thiobenzyl ester (Du Pont)--with both a discrete (aca) and a centrifugal analyzer (COBAS BIO). The correlation between the...

journal_title：Clinical chemistry

authors： Prellwitz W,Schmitt KF,Machner M,Schuster CJ,Weilemann L

更新日期：1982-11-01 00:00:00

abstract：BACKGROUND:24,25-Dihydroxyvitamin D [24,25(OH)2D] in serum may be both a nuisance and nutritionally valuable. METHODS:We investigated the impact of 24,25(OH)2D3 on the performance of commercially available immunoassays for serum total 25-hydroxyvitamin D [25(OH)D] using (a) serum from a nationally representative sampl...

journal_title：Clinical chemistry

authors： Cashman KD,Hayes A,Galvin K,Merkel J,Jones G,Kaufmann M,Hoofnagle AN,Carter GD,Durazo-Arvizu RA,Sempos CT

更新日期：2015-04-01 00:00:00

abstract：:A method has been developed for the determination of inorganic arsenic [As(III) and As(V)] and its organic metabolites (monomethylarsenic and dimethylarsenic) in urine by flow-injection hydride generation atomic absorption spectrometry. The nontoxic seafood-derived arsenobetaine and arsenocholine species were first se...

journal_title：Clinical chemistry

authors： Hanna CP,Tyson JF,McIntosh S

更新日期：1993-08-01 00:00:00

abstract：:This method for determining aluminum in human serum involves electrothermal atomic absorption spectrophotometry. A longer drying time allows less pre-dilution of the sample, and the method also includes a flush cycle after atomization. Standard-addition methodology is used to eliminate matrix effects and the need for ...

journal_title：Clinical chemistry

authors： Bertholf RL,Brown S,Renoe BW,Wills MR,Savory J

更新日期：1983-06-01 00:00:00

abstract：BACKGROUND:Biochemical markers of bone turnover can provide prognostic information about the risk of osteoporotic fracture and are useful tools for monitoring efficacy of antiresorptive therapy. A serum-based automated assay may be of better clinical value than urinary markers because of lower imprecision and day-to-da...

journal_title：Clinical chemistry

authors： Garnero P,Borel O,Delmas PD

更新日期：2001-04-01 00:00:00

abstract：:A recent study has shown that an emission-excitation matrix could be used to provide a unique fingerprint for the selective identification and characterization of certain species of Pseudomonas. This paper describes the results of a systematic study of the variables that contribute to the uniqueness of the fingerprint...

journal_title：Clinical chemistry

authors： Shelly DC,Warner IM,Quarles JM

更新日期：1980-09-01 00:00:00