Abstract：
:The ultrastructural localization of AChE and BuChE was studied in motor endplates of the rat and mouse diaphragms, using the CNS(?) modification of the one-step Cu-thiocholine procedure. AChE activity was observed on pre- and postsynaptic membranes, in the basal lamina and in the Schwann cell-nerve terminal interspace. The localization of BuChE was similar but much less pronounced in the adult muscle, while for the developing muscle, the reverse was true. In developing motor endplates and in endplates of rats after irreversible inhibition of AChE where synthesis de novo is enhanced, AChE activity in the endoplasmic reticulum and perinuclear membrane of the Schwann cell and in the sarcoplasmic reticulum was pronounced, indicating that junctional AChE may be synthesized at these sites. AChE and BuChE have a similar localization, mode of attachment and molecular forms. A possible mechanism underlying these similarities is discussed.
journal_name
Neurochem Intjournal_title
Neurochemistry internationalauthors
Brzin M,Sketelj J,Grubi Z,Kiauta Tdoi
10.1016/0197-0186(80)90021-2subject
Has Abstractpub_date
1980-01-01 00:00:00pages
149-59eissn
0197-0186issn
1872-9754pii
0197-0186(80)90021-2journal_volume
2Cpub_type
杂志文章abstract：:GABAA receptors mediate most of the fast inhibitory transmissions in the central nervous system. These receptors are pentameric complexes that exhibit high structural and pharmacological heterogeneity, as they can be constructed from 19 distinct subunits. GABAA receptors are the targets of numerous clinically relevant...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2018.05.015
更新日期：2018-09-01 00:00:00
abstract：:Catalpol has been shown to rescue neurons from kinds of damage in vitro and in vivo in previous reports. However, the effect of catalpol on the nitric oxide (NO) system via MAPKs signaling pathway of mesencephalic neurons largely remains to be verified. The current study examined that whether catalpol modulated NO and...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2008.12.003
更新日期：2009-03-01 00:00:00
abstract：:Cerebellar granule neurons produce homogenous cultures that provide a unique opportunity for quantifying the apoptosis by propidium iodide- and deoxynucleotidyl transferase-flow cytometry combined analysis and for studying its regulation by neurotrophins. Nerve growth factor (NGF) was found to promote postmitotic surv...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(96)00147-7
更新日期：1997-08-01 00:00:00
abstract：:The effects of ?-amino-n-butyric acid (GABA), (+)bicuculline, isoguvacine and 3-(4-chlorophenyl)-4-aminobutyrate [(+/-)baclofen] on the K-induced release of [(3)H]acetylcholine (ACh) were studied in the superior cervical ganglia of the rat in vitro. GABA and isoguvacine inhibited [(3)H]ACh release and these inhibition...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(86)90193-2
更新日期：1986-01-01 00:00:00
abstract：:The mobilization of inositol triphosphate ip3 by N-methyl D-aspartate (NMDA) and kainate, two excitatory amino acid EAA receptor agonists, was studied in cultured chick retina cells as a function of culture differentiation. Kainate (EC50 = 30 microM) stimulated from 6 to 9-fold the production of [3H]ip3 between E8C3 (...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(94)00143-i
更新日期：1995-04-01 00:00:00
abstract：:The disposition of labelled [3H]GM1lactone, the inner ester of ganglioside GM1, was studied in the rat. After i.v. administration [3H]GM1lactone was quickly converted to its corresponding open form most likely by plasma esterases, and then displayed a pharmacokinetic profile identical to [3H]GM1. Following intramuscul...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(92)90050-2
更新日期：1992-04-01 00:00:00
abstract：:Fluorescence measurements and singlet singlet energy transfer experiments on endothelin-1 provide information on the conformation of this peptide in dilute aqueous solution. The tyrosine fluorescence quantum yield in the absence of transfer (in [Phe(21)]endothelin-1) is relatively large (?(tyr) = 0.39), indicating the...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(91)90144-3
更新日期：1991-01-01 00:00:00
abstract：:Succinyl-Leu-Leu-Val-Tyr-4-methylcoumaryl-7-amide (Suc-LLVY-MCA) hydrolyzing activities of the 20S and 26S proteasomes in the gerbil cortex following transient forebrain ischemia were examined. Using extraction solutions without ATP, only 20S proteasome activity was noted after separation with glycerol gradient centri...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(95)00071-2
更新日期：1996-02-01 00:00:00
abstract：:Nerve terminals from the head ganglia of Locusta migratoria were isolated by means of a modified microscale flotation technique. Enzymatic, ultrastructural and chemical analysis revealed that the synaptosomal fraction was highly enriched in well-preserved nerve endings containing almost no free mitochondria. Cholinerg...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(81)90035-8
更新日期：1981-01-01 00:00:00
abstract：:The purpose of this study was to investigate the effect of (3S)-7-chloro-3-[2-((1R)-1-carboxyethoxy)-4-aminomethylphenyl]aminocarbonylmethyl-1,3,4,5-tetrahydrobenz[c,d]indole-2-carboxylic acid hydrochloride (SM-31900), an antagonist with high selectivity and affinity for the NMDA receptor glycine-binding site, on the ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(02)00137-7
更新日期：2003-04-01 00:00:00
abstract：:Increased brain ammonia concentrations are a hallmark feature of several neurological disorders including congenital urea cycle disorders, Reye's syndrome and hepatic encephalopathy (HE) associated with liver failure. Over the last decade, increasing evidence suggests that hyperammonemia leads to alterations in the gl...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/s0197-0186(03)00043-3
更新日期：2003-09-01 00:00:00
abstract：:Cyclothiazide has been shown to block desensitization of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-preferring receptors and to enhance quisqualate-, AMPA- and kainate-induced neurotoxicity. The pharmacology behind this cyclothiazide-enhanced kainate-induced excitotoxicity was characterized in em...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(97)00098-3
更新日期：1998-03-01 00:00:00
abstract：:The neonatal administration of 5,7-dihydroxytryptamine to rats (100 mg kg(?1) s.c. on the 1st and 2nd day after birth) resulted in marked reductions in serotoninergic presynaptic markers ([(3)H]-5-HT synaptosomal uptake, tryptophan hydroxylase activity and endogenous 5-HT content) in various forebrain areas, particula...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(81)90051-6
更新日期：1981-03-01 00:00:00
abstract：:Ligand-induced changes in the conformation of extracellular loop (EL) 2 in the rat (r) dopamine transporter (DAT) were examined using limited proteolysis with endoproteinase Asp-N and detection of cleavage products by epitope-specific immunoblotting. The principle N-terminal fragment produced by Asp-N was a 19kDa pept...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2013.11.003
更新日期：2014-07-01 00:00:00
abstract：:In order to compare the incorporation of several saturated fatty acids into the brain, radioactive palmitic, stearic and lignoceric acids were injected into mice. The radioactivity was measured in lipids from isolated neurons, astrocytes and myelin. Our data indicate that specific radioactivity of lignoceric acid afte...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(81)90020-6
更新日期：1981-01-01 00:00:00
abstract：:Neurosteroids are modulators of several receptors and ion channels and are implicated in the pathophysiology of several neuropsychiatric diseases including hepatic encephalopathy (HE). The neurosteroid, allopregnanolone, a positive allosteric modulator of GABA(A) receptors, accumulates in the brains of HE patients whe...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2005.12.006
更新日期：2006-06-01 00:00:00
abstract：:NADPH derived from glucose-6-phosphate dehydrogenase (G6PD), the rate-limiting enzyme of the pentose phosphate pathway, has been implicated not only to promote reduced glutathione (GSH) but also enhance oxidative stress in specific cellular conditions. In this study, the effects of G6PD antisense oligodeoxynucleotides...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2012.05.003
更新日期：2012-07-01 00:00:00
abstract：:The aim of this study was to investigate the involvement of calmodulin in phospholipase D activation in SH-SY5Y cells. Cells prelabelled with [3H]-palmitic acid were incubated with calmodulin antagonists and/or other compounds. Phosphatidylethanol, a specific marker for phospholipase D activity, and phosphatidic acid ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(01)00067-5
更新日期：2002-03-01 00:00:00
abstract：:(RS)-2-Amino-3-(3-hydroxy-5-phenyl-4-isoxazolyl)propionic acid (APPA), which is an analogue of (RS)-2-amino-3-(3-hydroxy-5-methyl-4-isoxazolyl)propionic acid (AMPA), shows the characteristics of a partial AMPA receptor agonist. Since (S)-APPA is a full AMPA agonist and (R)-APPA a competitive antagonist, the partial ag...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(95)00151-4
更新日期：1996-09-01 00:00:00
abstract：:In the last few years, several attempts were made for the setting up of specific methodologies aiming to evaluate steroid contents directly in nervous tissues. The main objective of these attempts was to determine accurately changes intervening in endogenous neuroactive steroid levels during pathological situations. T...
journal_title：Neurochemistry international
pub_type： 杂志文章,评审
doi：10.1016/j.neuint.2007.09.010
更新日期：2008-03-01 00:00:00
abstract：:Monocarboxylate transporter (MCT1) levels in brains of adult Long-Evans rats on a high-fat (ketogenic) diet were investigated using light and electron microscopic immunocytochemical methods. Rats given the ketogenic diet (91% fat and 9% protein) for up to 6 weeks had increased levels of the monocarboxylate transporter...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(00)00102-9
更新日期：2001-05-01 00:00:00
abstract：:Brain cortex synaptoneurosomes actively incorporated [14C]arachidonic acid (AA) into lipids. Serotonin (5-HT), at a concentration range of 10 microM-1 mM, significantly stimulates the incorporation of AA mainly into phosphatidylinositol (PI) of brain cortex synaptoneurosomes. The stimulation rate of AA incorporation b...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(94)90039-6
更新日期：1994-08-01 00:00:00
abstract：:The ventral pallidum (VP) is a critical component of the basal ganglia neurocircuitry regulating learning and decision making; however, its precise role in controlling associative learning of environmental stimuli conditioned to appetitive or aversive outcomes is still unclear. Here, we investigated the expression of ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2019.02.011
更新日期：2019-06-01 00:00:00
abstract：:The consequences of irreversibly-inhibiting D2 dopaminergic receptors on the expression of D1 and D2 dopamine receptor mRNAs and proenkephalin mRNA and on the levels of mu- and delta-opioid receptors in rat striatum were studied following single or repeated administration of the irreversibly-acting D2 dopamine recepto...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(94)90143-0
更新日期：1994-10-01 00:00:00
abstract：:Platelet-activating factor (PAF), a biologically active lipid (1-O-alkyl-2-acetyl-sn-glycero-3-phosphoholine), is identified in different regions of brain, including hippocampus. Specific PAF-activated receptors (PAFRs) are expressed in corresponding brain areas. PAF has been proposed to be a retrograde messenger of l...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(03)00126-8
更新日期：2004-02-01 00:00:00
abstract：:The saturable and specific high-affinity uptake of [(3)H]serotonin ([(3)H]5HT) (5 x 10(?8) M) was studied in slices from the hippocampus, parietal cortex, septum-preoptic area, and hypothalamus of male 2, 6, 12 and 24-32 month old C57BL/6N mice. Hippocampal [(3)H]5-HT uptake showed a significant biphasic relationship ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(83)90006-2
更新日期：1983-01-01 00:00:00
abstract：:Alpha-synuclein (ASN), a 140-amino acid protein, is richly expressed in presynaptic terminals in the central nervous system, where it plays a role in synaptic vesicle function. However, if it is altered and accumulated it is involved in neurodegeneration as Parkinson's disease (PD). ASN contained 35-amino acid domain ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2006.01.025
更新日期：2006-09-01 00:00:00
abstract：:Striatal quinolinic acid lesions have been used as a model for the pathology seen in Huntington's disease. Seven days following a unilateral injection of 100 nmol of quinolinic acid into the rat caudate-putamen (striatum), a large increase in the binding of [3H]WIN 35,428 to the lesioned caudate-putamen was seen when ...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/0197-0186(92)90091-5
更新日期：1992-12-01 00:00:00
abstract：:The aim of the present study was to investigate, whether dopamine D1 and/or D2 receptors are able to interfere with the ATP-induced increase of the intracellular Ca2+ concentration ([Ca2+]i) in cultured striatal neurons identified by their morphological characteristics and their [Ca2+]i transients in response to a hig...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/j.neuint.2007.06.013
更新日期：2008-01-01 00:00:00
abstract：:Potassium bisperoxo(1,10-phenantroline)oxovanadate (V) [bpV(phen)] is a potent protein tyrocine phosphatase inhibitor which mediates a variety of biological effects. The aim of these studies was to examine the role(s) of mitogen activated protein kinase (MAPK) pathways in PC12 cell proliferation and toxicity by bpV(ph...
journal_title：Neurochemistry international
pub_type： 杂志文章
doi：10.1016/s0197-0186(99)00018-2
更新日期：1999-04-01 00:00:00
