Abstract：
:Influenza H3N2 viruses have recently drifted from A/Wisconsin/67/05-like to A/Brisbane/10/07-like viruses. The effect of key amino acid substitutions in the hemagglutinin (HA) protein on the replication, antigenicity and immunogenicity of cold-adapted, live attenuated vaccine strains was examined. A/Brisbane/10/07 egg isolate contained a unique combination of G186 and P194 which were required for efficient virus growth in Madin-Darby Canine Kidney (MDCK) cells and embryonated chicken eggs, but the virus induced low level of serum antibody response in ferrets. Substitution of the G186 and P194 in the HA of A/Brisbane/10/07 by V186 and L194 that were present in the HA of A/Wisconsin/67/05-like viruses significantly impaired virus replication but greatly improved the immunogenicity of the vaccine virus to a level comparable to that elicited by the A/Wisconsin/67/05 vaccine. The replication of the variants with impaired growth could be improved by amino acid substitutions at the 195 or 226 residues. The viruses with the G186 and P194 residues were antigenically distinct from the viruses with V186 and L194. Sequence analysis of the HA sequences of the H3N2 viruses from the database and sequencing of the HA gene of a cell-derived A/Brisbane/10/07-like virus before and after egg passage indicated that the P194 residue was most likely derived from egg adaptation. Our results demonstrated the importance of careful evaluation of vaccine strains to ensure that the selected vaccines not only replicate well in eggs, but also retain their antigenicity and are immunogenic in the host.
journal_name
Vaccinejournal_title
Vaccineauthors
Chen Z,Zhou H,Jin Hdoi
10.1016/j.vaccine.2010.03.078subject
Has Abstractpub_date
2010-05-28 00:00:00pages
4079-85issue
24eissn
0264-410Xissn
1873-2518pii
S0264-410X(10)00478-0journal_volume
28pub_type
杂志文章相关文献
VACCINE文献大全abstract：:Development of effective new mucosal vaccine adjuvants has become a priority with the increase in emerging viral and bacterial pathogens. We previously reported that cationic liposomes complexed with non-coding plasmid DNA (CLDC) were effective parenteral vaccine adjuvants. However, little is known regarding the abili...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.05.009
更新日期：2011-07-18 00:00:00
abstract：:Many influenza vaccines targeted to hemagglutinin (HA) show efficient immunogenicity for protecting subjects against influenza virus infection. Major antigenic changes to HA molecules can help influenza virus to develop resistance against HA-targeted vaccines. DNA vaccines encoding conserved antigens protect animals a...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2007.02.074
更新日期：2007-05-22 00:00:00
abstract：:In clinical trials recombinant-modified vaccinia virus Ankara expressing the Mycobacterium tuberculosis antigen 85A (MVA85A) induces approximately 10 times more effector T cells than any other recombinant MVA vaccine. We have found that in BCG primed subjects MVA85A vaccination reduces transforming growth factor beta ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.07.040
更新日期：2008-09-26 00:00:00
abstract：BACKGROUND:Vaccination against Haemophilus influenzae type b (Hib) is included in routine pediatric immunization schedule in the United States. Previous vaccine shortages have created the need for additional options for Hib vaccination. METHODS:This phase III, randomized, multi-centered study (NCT01000974) evaluated t...
journal_title：Vaccine
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.vaccine.2017.05.018
更新日期：2017-06-16 00:00:00
abstract：:Although the USA is considered an area of 'low' endemicity for hepatitis B infection, the incidence of new cases, the prevalence of carriers, and the burden of acute and chronic disease place hepatitis B among the most important communicable diseases. It is estimated that 300,000 new cases of hepatitis B infection occ...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(95)80040-k
更新日期：1995-01-01 00:00:00
abstract：:The immunogenicity and protective efficacy of a nucleic acid vaccine encoding the herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) gene under the control of the CMV immediate early gene promoter was examined. Mice immunized three times by intramuscular injection with the vaccine developed an HSV specific IgG bu...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(96)00027-8
更新日期：1996-09-01 00:00:00
abstract：:Salmonella enterica serovar Typhimurium is a versatile host organism for the generation of recombinant live vaccines for mucosal immunization. We investigated the performance of an intracellular-activated promoter for expression of heterologous antigens by Salmonella carrier strains. We observed that intracellular exp...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2004.11.035
更新日期：2005-04-08 00:00:00
abstract：:Vaccination is the principal means used to control and treat porcine reproductive and respiratory syndrome virus (PRRSV) infection. An array of PRRS vaccine products is available in various regions of the world. However, despite extensive efforts, little progress has been made to improve efficacy since the first intro...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2011.09.013
更新日期：2011-10-26 00:00:00
abstract：:Current available influenza vaccines are safe and effective in preventing influenza. Nevertheless, there is a need for influenza vaccines with improved efficacy in the elderly. This need is underscored by both the observation that influenza has a major clinical and economic impact in the elderly and the fact that curr...
journal_title：Vaccine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.vaccine.2005.04.020
更新日期：2005-07-08 00:00:00
abstract：:Leishmania donovani promastigote soluble antigens (sLAg) were encapsulated in non-phosphatidylcholine (non-PC) liposomes (escheriosomes) prepared from E. coli lipids. The escheriosome-based vaccine was investigated for its potential to elicit a protective immune response against experimental visceral leishmaniasis. Th...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.10.025
更新日期：2006-03-10 00:00:00
abstract：:Vaccine safety fears following media reports of adverse events led to low (50.3%) coverage in a supplementary measles-rubella immunization campaign in Georgia in 2008. Review of adverse events associated with the campaign identified 432 reports (<0.1% of ∼ 493,000 vaccinees) including 338 (78.2%) cases of syncope. The...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.07.043
更新日期：2010-09-07 00:00:00
abstract：:Despite the extensive use of efficacious pertussis vaccines, Bordetella pertussis infections are still among the main causes for childhood morbidity and mortality. Severe pertussis occurs mostly in very young children, often too young to be sufficiently protected by current vaccines, which require several administrati...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.08.018
更新日期：2008-10-23 00:00:00
abstract：:The vaccination of wild animals against rabies has been developed most extensively in Europe. Experiments have demonstrated the efficacy of a vaccinia-rabies recombinant virus administered by the oral route in foxes. The innocuity of this vaccine was tested in the target species as well as in several non-target wild a...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/0264-410x(90)90129-a
更新日期：1990-04-01 00:00:00
abstract：:The candidate vaccine virus NIBRG-14 was derived by reverse genetics and comprises the haemagglutinin (HA) and neuraminidase (NA) genes derived from the clade 1 virus A/Viet Nam/1194/2004 on an A/Puerto Rico/8/34 (PR8) backbone. The HA gene was modified to remove the multibasic cleavage site motif associated with high...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2010.09.006
更新日期：2010-11-23 00:00:00
abstract：:Two rotavirus vaccines have been available since 2006. This study evaluates the effectiveness of these vaccines using a test-negative case-control design in Navarre, Spain. We included children 3-59 months of age who sought medical care for gastroenteritis and for whom stool samples were taken between January 2008 and...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.11.071
更新日期：2012-01-11 00:00:00
abstract：:Actinobacillus pleuropneumoniae is a gram negative pleiomorphic rod that is the causative agent of a severe, highly infectious and often fatal pleuropneumonia in swine. We have previously reported the construction of genetically-defined stable riboflavin auxotrophs by replacement of a portion of the APP riboflavin bio...
journal_title：Vaccine
pub_type： 临床试验,杂志文章
doi：10.1016/s0264-410x(00)00076-1
更新日期：2000-06-15 00:00:00
abstract：:Pre- and postexposure prophylaxis against hepatitis A virus (HAV) infection with immune serum globulin (Ig) is only effective for 4-6 months. We compared the safety, tolerability and immunogenicity of a single i.m. injection of Ig with a single and booster dose of an inactivated hepatitis A virus vaccine (iHAV) in adu...
journal_title：Vaccine
pub_type： 临床试验,杂志文章
doi：10.1016/0264-410x(93)90151-m
更新日期：1993-01-01 00:00:00
abstract：:The protective effect of purified human immunoglobulin against human T-cell leukaemia virus type I (HTLV-I), designated ATLIG on HTLV-I infection was examined in cynomolgus monkeys (Macaca fascicularis) as a preclinical study. Passive immunization of ATLIG 24 h before challenging HTLV-I protected the monkeys from HTLV...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(97)00055-8
更新日期：1997-08-01 00:00:00
abstract：:We evaluated the effectiveness of the Jeryl Lynn strain vaccine in a large outbreak of mumps in Navarre, Spain, 2006-2008. Each of the 241 cases of mumps occurring in children over 15 months of age born between 1998 and 2005 was compared with 5 controls individually matched by sex, birth date, district of residence an...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2009.02.001
更新日期：2009-03-26 00:00:00
abstract：:This study investigated predictors of influenza vaccination uptake since the introduction of the policy to target over 65s. Four hundred and forty-four participants completed a cross-sectional structured interview. The predictors of vaccination uptake were: having a doctor or nurse who explained why the vaccination is...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2004.12.031
更新日期：2005-05-20 00:00:00
abstract：:The immunogenicity of the live oral hybrid vaccine organism Salmonella typhi Ty21a/V. cholerae Inaba (EX210) following its growth in media containing variable concentrations of supplemental galactose was examined in human volunteer subjects. The local intestinal IgA-specific antibody responses to both typhoid and chol...
journal_title：Vaccine
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/0264-410x(91)90039-9
更新日期：1991-07-01 00:00:00
abstract：:Fish has a soluble form of TLR5 ortholog (TLR5S), which does not exist in mammals. We identified TLR5S from rainbow trout and named rtTLR5S, which was about 38% homologous to the extracellular domains of human (hu) and mouse TLR5. Adjuvancy of rtTLR5S to flagellin response by human TLR5 (huTLR5) was tested in this stu...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.11.003
更新日期：2006-03-15 00:00:00
abstract：:Intramuscular immunization of mice with DNA cocktail vaccines, comprising potential protective antigens P36, P46, NrdF, and P97or P97R1 of Mycoplasma hyopneumoniae, induced strong Th1-polarized immune responses against each antigen, with only P46 eliciting a serum IgG response. Subcutaneous immunization with protein c...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2008.06.005
更新日期：2008-08-12 00:00:00
abstract：:This randomized, double-blind trial conducted in the US and Canada was conducted to compare the safety and immunogenicity of two dosages of live attenuated oral monovalent G1 human rotavirus (HRV) vaccine administered orally at a 2-month interval concomitantly with routine childhood vaccines to healthy infants 5-15 we...
journal_title：Vaccine
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.vaccine.2005.07.030
更新日期：2006-05-01 00:00:00
abstract：:Infection with influenza virus during pregnancy poses a significant risk of complications for both mother and fetus. During the H1N1 2009 pandemic, pregnant women constituted one of the priority groups for vaccination in many countries, creating a need for close monitoring of the safety of the vaccine in pregnant wome...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.04.114
更新日期：2011-08-26 00:00:00
abstract：:Nucleic acid vaccination has many potential advantages over traditional methods, but suffers from the fact that DNA vaccines tend to be relatively poorly immunogenic. Attempts to enhance DNA vaccine immunogenicity have included the addition of cytokine-encoding plasmids into the formulation, as well as the use of hete...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2005.01.160
更新日期：2005-06-10 00:00:00
abstract：:A number of clinical reports of drug interactions with influenza vaccine have been made. We hypothesized that CYP3A4 activity would decrease following influenza immunization. Fifteen healthy subjects had erythromycin breath tests (ERMBT) and influenza antibody titer hemagglutinin inhibition assay (HIA) before and afte...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/s0264-410x(01)00403-0
更新日期：2001-12-12 00:00:00
abstract：:The emergence of lineage 2 strains of WNV in Europe as a cause of clinical disease and mortality in horses raised the question whether the existing WNV vaccines, all based on lineage 1 strains, protect against circulating lineage 2 strains of WNV. In the present paper we have determined the level of cross protection p...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.04.058
更新日期：2011-06-20 00:00:00
abstract：:The smallpox vaccine Vaccinia was successfully used to eradicate smallpox, but although very effective, it was a very reactogenic vaccine and responsible for the deaths of one or two people per million vaccinated. Modified Vaccinia virus Ankara (MVA) is a replication-deficient and attenuated derivative, also used in t...
journal_title：Vaccine
pub_type： 杂志文章,评审
doi：10.1016/j.vaccine.2013.03.020
更新日期：2013-09-06 00:00:00
abstract：:Conflicting findings regarding the level of protection offered by seasonal influenza vaccination against pandemic influenza H1N1 have been reported. We performed a test-negative case control study using sentinel patients from general practices in Victoria to estimate seasonal influenza vaccine effectiveness against la...
journal_title：Vaccine
pub_type： 杂志文章
doi：10.1016/j.vaccine.2011.03.055
更新日期：2011-08-26 00:00:00
