Abstract:
:The multi-functional cellulase gene, mfc, from Ampullaria crossean was transformed into Volvariella volvacea by PEG-mediated protoplast transformation to improve the biological efficiency and fruiting body yield. PCR and Southern blotting indicated that mfc was integrated into the genomes of four transformants. In laboratory and large scale cultivation experiments, the average biological efficiency of the transformants was 18.39+/-1.27% and 27.84+/-3.21%, respectively, considerably higher than the corresponding values for untransformed controls of 12.69+/-1.31% and 20.63+/-2.59%. This is the first report of an improvement in biological efficiency and fruiting body yield of V. volvacea through transgenesis.
journal_name
Bioresour Technoljournal_title
Bioresource technologyauthors
Zhao FY,Lin JF,Zeng XL,Guo LQ,Wang YH,You LRdoi
10.1016/j.biortech.2010.03.035subject
Has Abstractpub_date
2010-08-01 00:00:00pages
6482-6issue
16eissn
0960-8524issn
1873-2976pii
S0960-8524(10)00506-7journal_volume
101pub_type
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