Improvement in fruiting body yield by introduction of the Ampullaria crossean multi-functional cellulase gene into Volvariella volvacea.

Abstract:

:The multi-functional cellulase gene, mfc, from Ampullaria crossean was transformed into Volvariella volvacea by PEG-mediated protoplast transformation to improve the biological efficiency and fruiting body yield. PCR and Southern blotting indicated that mfc was integrated into the genomes of four transformants. In laboratory and large scale cultivation experiments, the average biological efficiency of the transformants was 18.39+/-1.27% and 27.84+/-3.21%, respectively, considerably higher than the corresponding values for untransformed controls of 12.69+/-1.31% and 20.63+/-2.59%. This is the first report of an improvement in biological efficiency and fruiting body yield of V. volvacea through transgenesis.

journal_name

Bioresour Technol

journal_title

Bioresource technology

authors

Zhao FY,Lin JF,Zeng XL,Guo LQ,Wang YH,You LR

doi

10.1016/j.biortech.2010.03.035

subject

Has Abstract

pub_date

2010-08-01 00:00:00

pages

6482-6

issue

16

eissn

0960-8524

issn

1873-2976

pii

S0960-8524(10)00506-7

journal_volume

101

pub_type

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