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In vivo silencing of Reptin blocks the progression of human hepatocellular carcinoma in xenografts and is associated with replicative senescence.

Abstract：

BACKGROUND & AIMS:We previously showed that Reptin is overexpressed in hepatocellular carcinoma (HCC), and that in vitro depletion of Reptin with siRNAs led to HCC cell growth arrest and apoptosis. Here, we asked whether in vivo targeting of Reptin in established tumours had a therapeutic effect. METHODS:We used lentiviral vectors to construct HuH7 and Hep3B cell lines with doxycycline (Dox)-dependent expression of Reptin (R2) or control shRNA (GL2). Cells were injected subcutaneously into immunodeficient mice, and Dox was given when tumours reached a volume of 250 mm(3). RESULTS:In vitro, the growth of GL2-Dox, GL2+Dox, and R2-Dox cells was undistinguishable whereas that of R2+Dox cells stopped 4 days after Dox treatment. The growth decrease was associated with increased apoptosis, and evidence of replicative senescence, as shown by staining for acid beta-galactosidase and the presence of senescence-associated heterochromatin foci. In xenografted mice, R2+Dox tumour growth stagnated or even regressed with prolonged treatment in contrast with the GL2-Dox, GL2+Dox, and R2-Dox tumours that progressed steadily. The blockage of tumour progression was associated with the induction of senescence and reduced cell proliferation. CONCLUSIONS:In vivo Reptin depletion leads to tumour growth arrest. Reptin may prove a valuable target in HCC.

journal_name

J Hepatol

journal_title

Journal of hepatology

authors

Ménard L,Taras D,Grigoletto A,Haurie V,Nicou A,Dugot-Senant N,Costet P,Rousseau B,Rosenbaum J

doi

10.1016/j.jhep.2009.12.029

subject

Has Abstract

pub_date

2010-05-01 00:00:00

pages

681-9

issue

5

eissn

0168-8278

issn

1600-0641

pii

S0168-8278(10)00092-9

journal_volume

52

pub_type

杂志文章

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