Voltage-gated sodium channels are required for heart development in zebrafish.

Abstract:

RATIONALE:Voltage-gated sodium channels initiate action potentials in excitable tissues. Mice in which Scn5A (the predominant sodium channel gene in heart) has been knocked out die early in development with cardiac malformations by mechanisms which have yet to be determined. OBJECTIVE:Here we addressed this question by investigating the role of cardiac sodium channels in zebrafish heart development. METHODS AND RESULTS:Transcripts of the functionally-conserved Scn5a homologs scn5Laa and scn5Lab were detected in the gastrulating zebrafish embryo and subsequently in the embryonic myocardium. Antisense knockdown of either channel resulted in marked cardiac chamber dysmorphogenesis and perturbed looping. These abnormalities were associated with decreased expression of the myocardial precursor genes nkx2.5, gata4, and hand2 in anterior lateral mesoderm and significant deficits in the production of cardiomyocyte progenitors. These early defects did not appear to result from altered membrane electrophysiology, as prolonged pharmacological blockade of sodium current failed to phenocopy channel knockdown. Moreover, embryos grown in calcium channel blocker-containing medium had hearts that did not beat but developed normally. CONCLUSIONS:These findings identify a novel and possibly nonelectrogenic role for cardiac sodium channels in heart development.

journal_name

Circ Res

journal_title

Circulation research

authors

Chopra SS,Stroud DM,Watanabe H,Bennett JS,Burns CG,Wells KS,Yang T,Zhong TP,Roden DM

doi

10.1161/CIRCRESAHA.109.213132

subject

Has Abstract

pub_date

2010-04-30 00:00:00

pages

1342-50

issue

8

eissn

0009-7330

issn

1524-4571

pii

CIRCRESAHA.109.213132

journal_volume

106

pub_type

杂志文章
  • Microvesicles as cell-cell messengers in cardiovascular diseases.

    abstract::Cell-cell communication has proven to be even more complex than previously thought since the discovery that extracellular vesicles serve as containers of biological information on various pathophysiological settings. Extracellular vesicles are classified into exosomes, microvesicles/microparticles, or apoptotic bodies...

    journal_title:Circulation research

    pub_type: 杂志文章,评审

    doi:10.1161/CIRCRESAHA.113.300858

    authors: Loyer X,Vion AC,Tedgui A,Boulanger CM

    更新日期:2014-01-17 00:00:00

  • Regulation of endothelial nitric oxide synthase expression by albumin-derived advanced glycosylation end products.

    abstract::We examined whether albumin-derived advanced glycosylation end products (AGEs) downregulate the expression of endothelial nitric oxide synthase (NOS). Significant reductions in NOS activity and cGMP levels in bovine aortic endothelial cells were observed when exposed to different concentrations of albumin-derived AGEs...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.86.3.e50

    authors: Rojas A,Romay S,González D,Herrera B,Delgado R,Otero K

    更新日期:2000-02-18 00:00:00

  • Hypertension following denervation of aortic baroreceptors in unanesthetized dogs.

    abstract::After cervical aortic nerve section, mean arterial pressure in the unanesthetized dog increased by an average of 7.4 mm Hg. Following a more extensive denervation of aortic arch receptors by section of intrathoracic vagal branches, arterial pressure increased by 16.7 mm Hg. The above changes were seen in the stable st...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.45.1.26

    authors: Ito CS,Scher AM

    更新日期:1979-07-01 00:00:00

  • Two types of calcium channels in single smooth muscle cells from rabbit ear artery studied with whole-cell and single-channel recordings.

    abstract::Freshly dispersed rabbit ear artery cells were studied using patch-clamp techniques to measure membrane currents in whole cells and single-channel currents in membrane patches. Whole-cell calcium currents recorded at physiologic extracellular calcium concentrations were small (approximately 10 pA). Recordings with 110...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:

    authors: Benham CD,Hess P,Tsien RW

    更新日期:1987-10-01 00:00:00

  • Myocardial function of the interventricular septum. Effects of right and left ventricular pressure loading before and after pericardiotomy in dogs.

    abstract::We examined the function of the interventricular septum in six open-chest dogs by inserting piezoelectric crystals into the interventricular septum. Continuous ultrasonic recordings showed that the changes in myocardial chord length (MCL) in the septum and free walls of the right and left ventricle were similar during...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.49.1.52

    authors: Molaug M,Stokland O,Ilebekk A,Lekven J,Kiil F

    更新日期:1981-07-01 00:00:00

  • Identification of myocardial and vascular precursor cells in human and mouse epicardium.

    abstract::During cardiac development, the epicardium is the source of multipotent mesenchymal cells, which give rise to endothelial and smooth muscle cells in coronary vessels and also, possibly, to cardiomyocytes. The aim of the present study was to determine whether stem cells are retained in the adult human and murine epicar...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/CIRCRESAHA.107.150755

    authors: Limana F,Zacheo A,Mocini D,Mangoni A,Borsellino G,Diamantini A,De Mori R,Battistini L,Vigna E,Santini M,Loiaconi V,Pompilio G,Germani A,Capogrossi MC

    更新日期:2007-12-07 00:00:00

  • Strongly binding myosin crossbridges regulate loaded shortening and power output in cardiac myocytes.

    abstract::This study investigated the possible roles of strongly binding myosin crossbridges in determining loaded shortening and power output in cardiac myocytes. Single skinned cardiac myocytes were attached between a force transducer and position motor, and shortening velocities were measured over a range of loads during var...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.87.9.768

    authors: McDonald KS,Moss RL

    更新日期:2000-10-27 00:00:00

  • Muscle ring finger 1, but not muscle ring finger 2, regulates cardiac hypertrophy in vivo.

    abstract::Muscle ring finger (MuRF) proteins have been implicated in transmitting mechanical forces to cell signaling pathways through their interactions with the giant protein titin. Recent evidence has linked mechanically-induced stimuli with the control of serum response factor activity and localization through MuRF2. This o...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.RES.0000259559.48597.32

    authors: Willis MS,Ike C,Li L,Wang DZ,Glass DJ,Patterson C

    更新日期:2007-03-02 00:00:00

  • Cardiovascular Disease in Women: Clinical Perspectives.

    abstract::Cardiovascular disease continues to be the leading cause of death among women in the United States, accounting for ≈1 of every 3 female deaths. Sex-specific data focused on cardiovascular disease have been increasing steadily, yet is not routinely collected nor translated into practice. This comprehensive review focus...

    journal_title:Circulation research

    pub_type: 杂志文章,评审

    doi:10.1161/CIRCRESAHA.116.307547

    authors: Garcia M,Mulvagh SL,Merz CN,Buring JE,Manson JE

    更新日期:2016-04-15 00:00:00

  • Nitric oxide increases cardiac IK1 by nitrosylation of cysteine 76 of Kir2.1 channels.

    abstract:RATIONALE:The cardiac inwardly rectifying K(+) current (I(K1)) plays a critical role in modulating excitability by setting the resting membrane potential and shaping phase 3 of the cardiac action potential. OBJECTIVE:This study aims to analyze the effects of nitric oxide (NO) on human atrial I(K1) and on Kir2.1 channe...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/CIRCRESAHA.109.197558

    authors: Gómez R,Caballero R,Barana A,Amorós I,Calvo E,López JA,Klein H,Vaquero M,Osuna L,Atienza F,Almendral J,Pinto A,Tamargo J,Delpón E

    更新日期:2009-08-14 00:00:00

  • Heart failure gene therapy: the path to clinical practice.

    abstract::Gene therapy, aimed at the correction of key pathologies being out of reach for conventional drugs, bears the potential to alter the treatment of cardiovascular diseases radically and thereby of heart failure. Heart failure gene therapy refers to a therapeutic system of targeted drug delivery to the heart that uses fo...

    journal_title:Circulation research

    pub_type: 杂志文章,评审

    doi:10.1161/CIRCRESAHA.113.300269

    authors: Pleger ST,Brinks H,Ritterhoff J,Raake P,Koch WJ,Katus HA,Most P

    更新日期:2013-08-30 00:00:00

  • Differential effects of digoxin at comparable concentrations in tissues of fetal and adult sheep.

    abstract::Electrocardiogram (ECG) electrodes and carotid arterial and superior vena caval (SVC) catheters were placed in eight nonpregnant ewes and 11 fetuses (109-129 days gestation) to measure heart rate, arterial presssure, P-R interval, left ventricular pre-ejection period (PEP), and left ventricular ejection time (LVET), b...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.41.5.635

    authors: Berman W Jr,Ravenscroft PJ,Sheiner LB,Heymann MA,Melmon KL,Rudolph AM

    更新日期:1977-11-01 00:00:00

  • Shear stress regulates forward and reverse planar cell polarity of vascular endothelium in vivo and in vitro.

    abstract::Cultured vascular endothelium displays profound morphological adaptations to shear stress that include planar cell polarity (PCP) that is directed downstream. Endothelial cells in blood vessels are also polarized; however, the direction of polarity is vessel specific, and shear-independent mechanisms have been inferre...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.RES.0000216595.15868.55

    authors: McCue S,Dajnowiec D,Xu F,Zhang M,Jackson MR,Langille BL

    更新日期:2006-04-14 00:00:00

  • Active oxygen species stimulate vascular smooth muscle cell growth and proto-oncogene expression.

    abstract::Vascular smooth muscle cells (VSMCs) proliferate in response to arterial injury. Recent findings suggest that, in addition to platelet-derived growth factors, growth factors from inflammatory cells and endothelial cells at the site of injury may contribute to VSMC proliferation. We hypothesized that a common mechanism...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.70.3.593

    authors: Rao GN,Berk BC

    更新日期:1992-03-01 00:00:00

  • Changes in vascular capacity in awake dogs in response to carotid sinus occlusion and administration of catecholamines.

    abstract::Changes in cardiac filling pressure (central venous pressure) were measured following carotid occlusion and infusions of catecholamines in awake dogs while cardiac output was held constant. After carotid occlusion in dogs with vagi blocked, central venous pressure increased about 0.8 mm Hg (an estimated decrease in va...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.55.4.440

    authors: Bennett TD,Wyss CR,Scher AM

    更新日期:1984-10-01 00:00:00

  • Acute renovascular hypertension in conscious dogs. Interaction of the renin-angiotensin system and sympathetic nervous system in systemic hemodynamics and regional blood flow responses.

    abstract::The effects of acute renovascular hypertension on the sympathetic nervous system, regional blood flow and cardiac function were studied in conscious dogs submitted to renal artery occlusion by inflation of a cuff implanted previously around one renal artery. We then compared the alterations in plasma renin and catecho...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.47.3.356

    authors: Gavras H,Liang C

    更新日期:1980-09-01 00:00:00

  • Progestogens do not affect aortic accumulation of cholesterol in ovariectomized cholesterol-fed rabbits.

    abstract::Cardiovascular disease is a major killer in postmenopausal women in the industrialized societies. To investigate the effect of progestogen and 17 beta-estradiol replacement therapy on atherogenesis, we studied 60 cholesterol-fed ovariectomized rabbits for 13 weeks. They were randomly assigned to four groups of 15 rabb...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.70.6.1198

    authors: Haarbo J,Svendsen OL,Christiansen C

    更新日期:1992-06-01 00:00:00

  • Aortic endothelial cell replication. I. Effects of age and hypertension in the rat.

    abstract::The daily rate of cell replication in the aortic endothelium of the normal rat falls from a maximum of 13% at birth to 0.1-0.3% at age 5-6 months. This residual rate of replication largely represents the turnover rate of the endothelium in normal animals. After renal hypertension of 2-3 weeks duration, however, the ra...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.41.2.248

    authors: Schwartz SM,Benditt EP

    更新日期:1977-08-01 00:00:00

  • Distinct behavior of cardiac myosin heavy chain gene constructs in vivo. Discordance with in vitro results.

    abstract::Transcriptional thyroid hormone responsiveness of the cardiac alpha-myosin heavy chain (alpha-MHC) gene has been demonstrated in transfections into fetal and neonatal cardiomyocytes and in transgenic mice. However, the correspondence between the regulation of MHC expression in dissociated cells with that in the intact...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.72.6.1211

    authors: Buttrick PM,Kaplan ML,Kitsis RN,Leinwand LA

    更新日期:1993-06-01 00:00:00

  • Vasorelaxation by red blood cells and impairment in diabetes: reduced nitric oxide and oxygen delivery by glycated hemoglobin.

    abstract::Vascular dysfunction in diabetes is attributed to lack of bioavailable nitric oxide (NO) and is postulated as a primary cause of small vessel complications as a result of poor glycemic control. Although it has been proposed that NO is bound by red blood cells (RBCs) and can induce relaxation of blood vessels distal to...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.RES.0000122044.21787.01

    authors: James PE,Lang D,Tufnell-Barret T,Milsom AB,Frenneaux MP

    更新日期:2004-04-16 00:00:00

  • Long-chain acylcarnitines mediate the hypoxia-induced increase in alpha 1-adrenergic receptors on adult canine myocytes.

    abstract::To elucidate the mechanisms responsible for the increase in alpha 1-adrenergic receptors during ischemia in vivo, we developed a procedure for measuring alpha 1-adrenergic receptors in isolated, calcium-tolerant adult canine myocytes. Specific [3H]prazosin binding was rapid, saturable, reversible, and demonstrated the...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.61.5.735

    authors: Heathers GP,Yamada KA,Kanter EM,Corr PB

    更新日期:1987-11-01 00:00:00

  • Biphasic restitution of action potential duration and complex dynamics in ventricular myocardium.

    abstract::The purpose of this study was to determine whether biphasic restitution of action potential duration (APD) in ventricular muscle permits the development of complex dynamic behavior. Such behavior is expected because of the steep ascending slope of restitution and the presence of a maximum. Action potentials recorded f...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.76.5.915

    authors: Watanabe M,Otani NF,Gilmour RF Jr

    更新日期:1995-05-01 00:00:00

  • Dyssynchronous Ca(2+) sparks in myocytes from infarcted hearts.

    abstract::The kinetics of contractions and Ca(2+) transients are slowed in myocytes from failing hearts. The mechanisms accounting for these abnormalities remain unclear. Myocardial infarction (MI) was produced by ligation of the circumflex artery in rabbits. We used confocal microscopy to record spatially resolved Ca(2+) trans...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.87.11.1040

    authors: Litwin SE,Zhang D,Bridge JH

    更新日期:2000-11-24 00:00:00

  • Effects of norepinephrine and angiotensin II on the determinants of glomerular ultrafiltration and proximal tubule fluid reabsorption in the rat.

    abstract::In 26 Wistar rats with surface glomeruli, the determinants of glomerular ultrafiltration and peritubular capillary uptake of proximal reabsorbate were studied before and during intravenous infusions of norepinephrine or angiotensin II. Regardless of whether renal perfusion pressure (AP) was permitted to increase, both...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.37.1.101

    authors: Myers BD,Deen WM,Brenner BM

    更新日期:1975-07-01 00:00:00

  • Length-induced changes in activation during contraction. A study of mechanical oscillations in strontium-mediated contractions of cat and frog heart muscle.

    abstract::The plateau phase of prolonged Sr-mediated contractions of preparations of cat and frog heart muscle was used to study the transient response to abrupt changes in load or length. An oscillatory response (total amplitude equal to or less than 5% Lmax) was obtained. Isotonic oscillations were less damped than their isom...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.38.4.289

    authors: Henderson AH,Cattell MR

    更新日期:1976-04-01 00:00:00

  • An angiotensin II type 1 receptor mutant lacking epidermal growth factor receptor transactivation does not induce angiotensin II-mediated cardiac hypertrophy.

    abstract::We have shown previously that tyrosine 319 in a conserved YIPP motif in the C terminus of angiotensin II (Ang II) type 1 receptors (AT(1)Rs) is essential for transactivation of epidermal growth factor receptor (EGFR) in vitro. We hypothesized that the signaling mechanism mediated through the specific amino acid sequen...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.RES.0000240147.49390.61

    authors: Zhai P,Galeotti J,Liu J,Holle E,Yu X,Wagner T,Sadoshima J

    更新日期:2006-09-01 00:00:00

  • Overview of pyridine nucleotides review series.

    abstract::Pyridine nucleotides are abundant soluble coenzymes and they undergo reversible oxidation and reduction in several biological electron-transfer reactions. They are comprised of two mononucleotides, adenosine monophosphate and nicotinamide mononucleotide, and are present as oxidized and reduced nicotinamide adenine din...

    journal_title:Circulation research

    pub_type: 杂志文章,评审

    doi:10.1161/CIRCRESAHA.111.247924

    authors: Nakamura M,Bhatnagar A,Sadoshima J

    更新日期:2012-08-17 00:00:00

  • Reversibly injured, postischemic canine myocardium retains normal contractile reserve.

    abstract::Transient coronary occlusion (15 minutes) does not result in irreversible myocardial injury but is associated with a depression of contractile function sustained for several hours to days ("stunned myocardium"). The defect in the contractile process responsible for this phenomenon has been suggested to be causally rel...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.61.6.834

    authors: Ito BR,Tate H,Kobayashi M,Schaper W

    更新日期:1987-12-01 00:00:00

  • Cardiovascular consequences when nitric oxide and lipid signaling converge.

    abstract::The identification of nitric oxide ((*)NO) as an endogenously produced free radical mediator of endothelial-dependent relaxation and host defense has fundamentally changed concepts of cell signal transduction. Ligand-receptor oriented paradigms of cell signaling were originally centered on the concept of a high affini...

    journal_title:Circulation research

    pub_type: 杂志文章,评审

    doi:10.1161/CIRCRESAHA.109.202077

    authors: Rudolph V,Freeman BA

    更新日期:2009-09-11 00:00:00

  • Enrichment, solubilization, and partial characterization of digitonin-solubilized muscarinic receptors derived from canine ventricular myocardium.

    abstract::The subcellular distribution of cardiac muscarinic receptors was defined in canine ventricular myocardium, and receptors were solubilized from subcellular fractions enriched in muscarinic receptor content. The subcellular location of muscarinic receptors in cardiac tissue was determined by measurement of the distribut...

    journal_title:Circulation research

    pub_type: 杂志文章

    doi:10.1161/01.res.52.6.664

    authors: Manalan AS,Werth DK,Jones LR,Watanabe AM

    更新日期:1983-06-01 00:00:00